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Offlineshroombasa
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Anyone grow KO1 on straw?
    #23709491 - 10/05/16 10:30 AM (7 years, 3 months ago)

I'm trying to fruit a straw log of KO1 and it's just not having it.  Fully colonized, but won't thicken or pin, just kind of sitting there.  Has anyone else noticed this with this strain?


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InvisibleMarty Mycfly
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Re: Anyone grow KO1 on straw? [Re: shroombasa]
    #23710278 - 10/05/16 03:14 PM (7 years, 3 months ago)

I grow K01, but on supplemented sawdust, it's awesome, but can be tricky while getting used to it.
I have heard that kings don't do well at all on straw, I have seen mushfarmer on youtube growing them on straw logs but he would supplement the straw with alfalfa, which in the end was getting too high of a contam rate and low BE and switched his operation over to sterilized supplemented sawdust eventually.

With bag culture the bags need to be incubated for 2-3 weeks, and then to induce pinning the bags are moved to 50-60 degree temps for 5-7 days.
That is the big thing, they will only pin in those temps. Then to fruit, cut open the bag when the pins are growing good.

What temps are you running right now, that may be the issue?


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InvisibleRogerSmith

Registered: 01/29/15
Posts: 365
Re: Anyone grow KO1 on straw? [Re: Marty Mycfly]
    #23710366 - 10/05/16 03:56 PM (7 years, 3 months ago)

KO1 culture circling around is shit. Probably degenerated. I've done side by side comparison with it a few times


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Offlineshroombasa
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Re: Anyone grow KO1 on straw? [Re: Marty Mycfly]
    #23711839 - 10/05/16 11:46 PM (7 years, 3 months ago)

Quote:

Marty Mycfly said:
I grow K01, but on supplemented sawdust, it's awesome, but can be tricky while getting used to it.
I have heard that kings don't do well at all on straw, I have seen mushfarmer on youtube growing them on straw logs but he would supplement the straw with alfalfa, which in the end was getting too high of a contam rate and low BE and switched his operation over to sterilized supplemented sawdust eventually.

With bag culture the bags need to be incubated for 2-3 weeks, and then to induce pinning the bags are moved to 50-60 degree temps for 5-7 days.
That is the big thing, they will only pin in those temps. Then to fruit, cut open the bag when the pins are growing good.

What temps are you running right now, that may be the issue?



Thanks Marty.  They're fruiting between 58 and 64F.  Maybe I just need to switch to bags instead.  I also thing I might have expanded my spawn too far, so that could be the problem too.


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OfflineTravelAgency
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Re: Anyone grow KO1 on straw? [Re: shroombasa]
    #23713025 - 10/06/16 12:18 PM (7 years, 3 months ago)

I'm about to start K01 -

:threadmonitor:


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OfflineJanuaryWolf
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Re: Anyone grow KO1 on straw? [Re: TravelAgency]
    #23713089 - 10/06/16 12:42 PM (7 years, 3 months ago)

I'm trying KO1 on hanging straw logs (supplemented with alfalfa). It's pinning!:zomgzomgzomg:


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Offlineshroombasa
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Re: Anyone grow KO1 on straw? [Re: JanuaryWolf]
    #23713178 - 10/06/16 01:08 PM (7 years, 3 months ago)

Quote:

JanuaryWolf said:
I'm trying KO1 on hanging straw logs (supplemented with alfalfa). It's pinning!:zomgzomgzomg:



Nice!  How long from inoculation?


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Invisiblesolarity
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Re: Anyone grow KO1 on straw? [Re: shroombasa]
    #23713264 - 10/06/16 01:38 PM (7 years, 3 months ago)

I compared 4 strains of KO on sterile sub and KO1 was the worst performer - full colonisation - no fruits. This seems to correlate with others experience.


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InvisibleMarty Mycfly
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Re: Anyone grow KO1 on straw? [Re: solarity]
    #23713337 - 10/06/16 02:14 PM (7 years, 3 months ago)

Interesting, for those of you having no luck with K01, how did you get the culture?
Mine which has been excellent came from spawn from Aloha, I saved some grains to dishes an then to slants.


--------------------
Mycfly's King Oysters                                       

Mycfly's Reishi    

                                                 


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OfflineJanuaryWolf
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Re: Anyone grow KO1 on straw? [Re: Marty Mycfly]
    #23713471 - 10/06/16 03:00 PM (7 years, 3 months ago)

My culture came directly from Aloha 3 or 4 years ago, I've made my own plates and slants too.

These were inoculated 8/31, so 7 weeks 6 weeks ago. I put them straight into the fruiting chamber because I don't have a space to incubate straw logs. They all go straight from inoculation into the fruiting chamber. Temps were 68-75F but for the last 3 weeks they've been at 64-68F




These pictures are on their sides, so the bags are actually vertical.

edits:more info added


Edited by JanuaryWolf (10/06/16 03:38 PM)


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OfflineJanuaryWolf
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Re: Anyone grow KO1 on straw? [Re: shroombasa]
    #23713549 - 10/06/16 03:25 PM (7 years, 3 months ago)

How long ago did you inoculate the bag you're waiting on?


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Offlineshroombasa
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Re: Anyone grow KO1 on straw? [Re: Marty Mycfly]
    #23713973 - 10/06/16 06:07 PM (7 years, 3 months ago)

Quote:

solarity said:
I compared 4 strains of KO on sterile sub and KO1 was the worst performer - full colonisation - no fruits. This seems to correlate with others experience.



That's interesting and good to know.  Is there a culture you'd recommend for kings?
Quote:

Marty Mycfly said:
Interesting, for those of you having no luck with K01, how did you get the culture?
Mine which has been excellent came from spawn from Aloha, I saved some grains to dishes an then to slants.



I did the same thing, cloned spawn straight from Aloha.  Backed up with slants and petris.

I actually just noticed the logs are starting to pin, but I've got these strange mutant looking blobs going on too.  I've fruited two other species of kings before and the blobs definitely aren't normal.


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Offlineshroombasa
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Re: Anyone grow KO1 on straw? [Re: JanuaryWolf]
    #23713989 - 10/06/16 06:12 PM (7 years, 3 months ago)

I inoculated on 09/07.  It's probably a little bit of my impatience showing too, but they're taking longer to colonize and pin than clones I've gotten from grocery store fruits.  It's just kind of weird to me that the culture is expensive and apparently people are getting better results from $20 syringes.


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InvisibleMarty Mycfly
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Re: Anyone grow KO1 on straw? [Re: shroombasa]
    #23714329 - 10/06/16 08:26 PM (7 years, 3 months ago)

I don't know?
I grow them in bags and induce pinning after 3-4 weeks of incubation by placing them in a cold environment for 1 week, then open the bags, works every time.
I have tried to fruit blocks that I opened up before pinning and they never would fruit. Maybe it's just the way k01 likes to fruit, to me it seems the conjunction of the "cold shock" and the invitro pinning get's them going, but I'm sure some bad k01 cultures could also be going around as well.

Actually correction, I have had good success with K01 casing bags and fruiting straight from incubation into the grow room, but never uncased, uncased they will only pin in the bag.


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Mycfly's King Oysters                                       

Mycfly's Reishi    

                                                 


Edited by Marty Mycfly (10/06/16 08:29 PM)


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InvisibleMarty Mycfly
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Re: Anyone grow KO1 on straw? [Re: Marty Mycfly]
    #23714370 - 10/06/16 08:41 PM (7 years, 3 months ago)

Here are some bags, the cased ones were put directly into fruiting after casing and the uncased bags were taken out of incubation at the the same time but the bags were cut open after a week of pinning in low temps.


Here is some more K01












--------------------
Mycfly's King Oysters                                       

Mycfly's Reishi    

                                                 


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OfflineJanuaryWolf
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Re: Anyone grow KO1 on straw? [Re: shroombasa]
    #23715240 - 10/07/16 06:20 AM (7 years, 3 months ago)

Quote:

shroombasa said:
I inoculated on 09/07.  It's probably a little bit of my impatience showing too, but they're taking longer to colonize and pin than clones I've gotten from grocery store fruits.  It's just kind of weird to me that the culture is expensive and apparently people are getting better results from $20 syringes.



I have sawdust bags with K01 that were inoculated on 9/7 and not pinning, and seem close. That's only 5 weeks, and I think it just takes 6 weeks.


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Invisiblesolarity
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Re: Anyone grow KO1 on straw? [Re: Marty Mycfly]
    #23716439 - 10/07/16 01:40 PM (7 years, 3 months ago)

Quote:

Marty Mycfly said:
Here are some bags, the cased ones were put directly into fruiting after casing and the uncased bags were taken out of incubation at the the same time but the bags were cut open after a week of pinning in low temps.




:bow2:


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InvisibleMarty Mycfly
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Re: Anyone grow KO1 on straw? [Re: JanuaryWolf]
    #23716461 - 10/07/16 01:52 PM (7 years, 3 months ago)

With sawdust bags at least in my experience it's all about inducing the pinning, I would do a large batch of bags, 50 or so, and after 3 weeks of incubation I would put x amount of bags into cold conditions to pin, and they do within 5-7 days, the rest of the bags sit in incubation for another week until I pull more out to pin and ect... Kind of like shiitake they will only pin when put in pinning conditions but can sit and incubate for however much time until you decide to do so.


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Mycfly's King Oysters                                       

Mycfly's Reishi    

                                                 


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OfflineJanuaryWolf
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Re: Anyone grow KO1 on straw? [Re: Marty Mycfly]
    #23730579 - 10/12/16 09:06 AM (7 years, 3 months ago)

Not babies anymore...




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OfflineTravelAgency
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Re: Anyone grow KO1 on straw? [Re: JanuaryWolf]
    #23741237 - 10/16/16 12:43 AM (7 years, 3 months ago)

What did you use to case with Marty?

Nice looking fruits JW!


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