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OfflineMotherload
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Registered: 12/31/15
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Last seen: 5 years, 17 days
3 B+ WBS multispore croir/verm monotubs - opinions?
    #23708163 - 10/04/16 09:07 PM (7 years, 3 months ago)

I used a b+ syringe straight to wbs jars. Then I took the two best jars and made 7 Quarts from one and 12 Quart jars from the other.
Spawned the first 7 Quarts to one tub and 6 each to the other two.

I was planning on introducing the first tub to FAE in a couple more days.
Take a look and let me know what you think!
I will continue posting photos.

These photos are of the first 7 Quart tub the other two still have a while to go, so I will post photos of them later.





Ps
The tubs are at around 80 to 83 degrees should I go ahead and lower it to the mid 70s?


Edited by Motherload (10/04/16 09:51 PM)


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OfflineMotherload
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Registered: 12/31/15
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? *DELETED* [Re: Motherload]
    #23708213 - 10/04/16 09:21 PM (7 years, 3 months ago)

Post deleted by Motherload

Reason for deletion: Double post



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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Motherload]
    #23708273 - 10/04/16 09:35 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:59 AM)


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Offlinespore-ty
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Motherload]
    #23708488 - 10/04/16 10:47 PM (7 years, 3 months ago)

Quote:

amidogen said:
Yes. You also shouldn't double post. Read the rules. You can only bump your thread if no one has replied after 24 hours. Otherwise use the edit button.

PS - Don't put MS->grains.





Hes right about everything but



Ps
The tubs are at around 80 to 83 degrees should I go ahead and lower it to the mid 70s?






Yes go ahead and lower room temp is fine and light is beneficial to all stages of mycelium


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OfflineChristaphonZ
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Registered: 09/10/16
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: spore-ty]
    #23708548 - 10/04/16 11:25 PM (7 years, 3 months ago)

What is wrong with ms->wbs?? How else should a newer person start?


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OfflineGreg
always learning
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: ChristaphonZ] * 1
    #23708566 - 10/04/16 11:35 PM (7 years, 3 months ago)

MS -> agar -> whatever grain/seed is the preferred method.
(Followed by G2G, or whatever, as long as the culture is cleaned on agar first)

The problem with ms -> grain/seed is that spores are collected (and grown) in open air.
Open air has all sorts of nasties like mold and bacteria.
Inevitably some amount of those nasties end up in the spores and then in the spore syringe.

With agar you can spot them before they overtake the cubensis, then you can isolate the cube myc from the contams.
If you go straight from spores to grain, this is not the case.
You're likely to get bacteria, if not mold.

Bod's excellent guide to agar:
https://www.shroomery.org/forums/showflat.php/Number/21922023


Edited by Greg (10/05/16 12:43 AM)


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OfflineMotherload
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Greg]
    #23744160 - 10/16/16 10:56 PM (7 years, 3 months ago)



:rockon:

the other two still arent ready yet, but soon updates on that..

will update in a week  :bigblunt:


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Offlinewtfcrazymofo
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Motherload]
    #23744428 - 10/17/16 03:48 AM (7 years, 3 months ago)

Nice shroomies clone a pin from a pin cluster on agar!


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineMotherload
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: wtfcrazymofo]
    #23745158 - 10/17/16 10:40 AM (7 years, 3 months ago)

Yeah I was going to just order some pre-made plates in a week or two to start with, less to fuck up and costs basically the same it seems...

No monies atm or I would :frown:


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OfflinePortabellaFella 1
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Registered: 08/08/16
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Motherload]
    #23745187 - 10/17/16 10:48 AM (7 years, 3 months ago)

Careful with those preordered plates. I did the same thing and they were waaaaay too nutritious. My myc was fluff balls that grew nowhere.

Definitely not worth.


--------------------
I would like to acquire anything I don’t have


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OfflineMotherload
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: PortabellaFella 1]
    #23748516 - 10/18/16 10:30 AM (7 years, 3 months ago)

Which petri dishes do you recommend ordering? The glass are expensive for now..
Deep dish? I assume not Vented?


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OfflineGreg
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Motherload]
    #23748527 - 10/18/16 10:35 AM (7 years, 3 months ago)

I've been using these ones:
https://www.amazon.com/gp/product/B00UB01AQ2
I think they're vented, but they've been working great for me.

I've seen cheaper packs of 500 that other members posted, so hopefully someone can chime in with a link to those.
You can buy packs of 20 from many of our sponsors, but it's more expensive to buy those small packs in the long run.

There are ones with dividers, but I hate them. More effort to pour and the dividers don't really do much.

Make sure to pick up some parafilm also (2" wide roll). You can get rolls on Amazon or from one of Shroomery's sponsors.


Edited by Greg (10/18/16 10:46 AM)


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OfflineMotherload
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Greg]
    #23749030 - 10/18/16 01:53 PM (7 years, 3 months ago)

What do you use the parafilm for? Taping around the dishes to seal them?


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Offlineenlightenment
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Motherload]
    #23749035 - 10/18/16 01:55 PM (7 years, 3 months ago)

Yes it is for sealing the dishes. I would never do agar plates without parafilm. I love it and I recommend to use it. It helps a lot.


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Invisiblec10h12n2o
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Motherload]
    #23749205 - 10/18/16 03:01 PM (7 years, 3 months ago)

cheapest i have found is lcmlab.com, they have em in several sizes, the ones i get are around 50 for 500 vented, and they have really great deals on "teaching kits" that are little per-student sets of measuring beakers, etc, that are a steal. lots of good stuff, great prices

there are several science stores that sell less than 60$ for 500 vented, and the cheapest ones often sell out (lake charles lab restocks quite often). but yeah pretty much any supplier, as long as you are buying by the 500 case, it is waaaaaaay cheaper than buying a sleeve at a time, even if you paid $100 for it. i suggest getting a case + some good cheap glass and plastic labware till you hit the free shipping threshold at one of the science stores (or just go amazon/ebay). it saves so much time and money in the end it is crazy to buy less than 500 at a time, i wasted so much time and money lol

and parafilm is gods gift to lab techs. it is used for all kinds of stuff, but we chiefly use it for sealing the petri edges. lol i wasted a BUNCH that came with a yeast culture kit i had laying around, i read that it needs to be stretched so i stretched the whole sheet lol... i used to do tiny ziplocs exactly same size as petris, inside bigger ziplocs. that was soooo time consuming, now i just do parafilm and put inside a ziploc for extra protection

here is a great video that cleared up my misunderstanding of how to use parafilm:



--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineGreg
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Re: 3 B+ WBS multispore croir/verm monotubs - opinions? [Re: Motherload]
    #23749226 - 10/18/16 03:08 PM (7 years, 3 months ago)

Quote:

Motherload said:
What do you use the parafilm for? Taping around the dishes to seal them?




Click on the link in my signature titled "Proper parafilm usage" and watch the video linked above this comment.


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