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OfflineDanktopus
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Everything failed! Contaminations!
    #23705736 - 10/04/16 04:45 AM (7 years, 3 months ago)

I have recently started an LC operation and I can't seem to get it to work for me. I made 3 different LC jars PC'ed at 15psi for 20 min all with different lid styles trying to figure out which one I like the best. Knocked up with spores and have decent growth in all 3 jars 2 weeks later. I used three different syringes and needles (PC'ed at 15psi for 15 min)  flame sterilized before and after aspirating LC, into 9 different rye berry jars (3 of each LC). My rye berries are added a pinch of gypsum, soaked 24 hours then PC'ed at 15psi for 90 min. Now 3 days after inoculation every single jar has what appears to be the same contamination. My grain lids are self healing ports with synthetic filter discs BTW, and all products have been placed in a brand new incubator set to 77 degrees. Nothing is ever exposed to open air, the injection port I hit heavy with isopropyl befor and after injecting. What am I doing wrong? Please help and any advice is appreciated!
-Danktopus


--------------------
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Offlinethelivingfreekshow
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Re: Everything failed! Contaminations! [Re: Danktopus]
    #23705757 - 10/04/16 05:03 AM (7 years, 3 months ago)

Pics would help.  Also incubators are bad. 65 to 75f is fine for the entire life cycle for cubes. Do you have a lot of experience? Could this contam just be mycelium? Describe it if you cant upload pics.


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: thelivingfreekshow]
    #23705761 - 10/04/16 05:09 AM (7 years, 3 months ago)

I believe the problem is that you inoculated LC with spore syringes. Use agar then transfer clean growth to the LC.


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Offlinetump
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Re: Everything failed! Contaminations! [Re: PortabellaFella 1]
    #23705767 - 10/04/16 05:22 AM (7 years, 3 months ago)

Bad luck sometimes


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InvisibleTuhdoww
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Re: Everything failed! Contaminations! [Re: tump]
    #23705779 - 10/04/16 05:41 AM (7 years, 3 months ago)

Lc from spores


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Offlineshrumzen
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Re: Everything failed! Contaminations! [Re: PortabellaFella 1]
    #23705796 - 10/04/16 05:59 AM (7 years, 3 months ago)

Quote:

PortabellaFella 1 said:
I believe the problem is that you inoculated LC with spore syringes. Use agar then transfer clean growth to the LC.




I made LC from syringes as well and it worked fine for me. Also it should not be the reason for contamination.

Pictures of the problem would help.


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OfflineGreenskybluegrass7
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Re: Everything failed! Contaminations! [Re: shrumzen]
    #23705808 - 10/04/16 06:06 AM (7 years, 3 months ago)

Quote:

shrumzen said:
Quote:

PortabellaFella 1 said:
I believe the problem is that you inoculated LC with spore syringes. Use agar then transfer clean growth to the LC.




I made LC from syringes as well and it worked fine for me. Also it should not be the reason for contamination.

Pictures of the problem would help.




You just finished your first grow homie


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: shrumzen]
    #23705823 - 10/04/16 06:22 AM (7 years, 3 months ago)

Quote:

shrumzen said:
Quote:

PortabellaFella 1 said:
I believe the problem is that you inoculated LC with spore syringes. Use agar then transfer clean growth to the LC.




I made LC from syringes as well and it worked fine for me. Also it should not be the reason for contamination.

Pictures of the problem would help.




I'm pretty sure you got lucky. The failure rate from MS to LC is huge. All 3 show the same contam, the vector is inside the syringe as he used decent sterile techniques. But that's just from the 1000 posts I've read on the subject. But yes, a pic or two would help as he didn't describe what the contam looks like.


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Edited by PortabellaFella 1 (10/04/16 06:30 AM)


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Re: Everything failed! Contaminations! [Re: PortabellaFella 1]
    #23705842 - 10/04/16 06:41 AM (7 years, 3 months ago)

spores to LC is a bad bad way of germinating and expanding mycelium...


--------------------

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Offlinethelivingfreekshow
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Re: Everything failed! Contaminations! [Re: pablokabute]
    #23705890 - 10/04/16 07:13 AM (7 years, 3 months ago)

Quote:

pablokabute said:
spores to LC is a bad bad way of germinating and expanding mycelium...




Very true. That being said, I go print>syringe>Lc>Wbs>cvg 66qt mono. I used to use agar, but that got old. Ive done over 100  jars this year so far sans agar, with maybe 5 or 6 lost to contams . If that's luck, I'll take it.  I try to get the cleanest prints possible, and have honed my sterile technique over several years. Everyone should work with agar at least a few times tho, and most find it very helpful, Im just not one of them. Long story short, its probably your LC, and if youre short on experience, your sterile technique may need work. Pics will most times get you a quicker, more accurate answer.


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Re: Everything failed! Contaminations! [Re: thelivingfreekshow]
    #23705900 - 10/04/16 07:22 AM (7 years, 3 months ago)

Alright... I'm an asshole but I'm not usually this mean... But I've had enough.  Nobody fucking cares how many of you have gotten away with it.  That is so far removed from the entire point.

Think about it for 5 goddamn seconds.  How are spores obtained?  When a fruit is near producing spores, what kind of shit is floating around in the air at the same time?  What's to stop those shits having a field day in your sterilized sugar water?

If you haven't even started fucking with agar you need to put the sugar water away and start understanding what's going on with your cultures.  You probably think everything went fine because you can't even spot bacteria yet.  Seriously, if you've done less than 5 grows, and you think your sample set is indicative of anything, just shut up and save the advice-giving for people who've been around the block enough to fail and figure out why before getting back on the road to success.

I can count on one hand the number of times anybody has benefited from a noob's perspective on LC.  In fact, I could probably still do it if I caught a live hand grenade.


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InvisibleTuhdoww
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705904 - 10/04/16 07:26 AM (7 years, 3 months ago)

Quote:

Inocuole said:
Alright... I'm an asshole but I'm not usually this mean... But I've had enough.  Nobody fucking cares how many of you have gotten away with it.  That is so far removed from the entire point.

Think about it for 5 goddamn seconds.  How are spores obtained?  When a fruit is near producing spores, what kind of shit is floating around in the air at the same time?  What's to stop those shits having a field day in your sterilized sugar water?

If you haven't even started fucking with agar you need to put the sugar water away and start understanding what's going on with your cultures.  You probably think everything went fine because you can't even spot bacteria yet.  Seriously, if you've done less than 5 grows, and you think your sample set is indicative of anything, just shut up and save the advice-giving for people who've been around the block enough to fail and figure out why before getting back on the road to success.

I can count on one hand the number of times anybody has benefited from a noob's perspective on LC.  In fact, I could probably still do it if I caught a live hand grenade.




I been waiting for you noc:rofl:


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: thelivingfreekshow]
    #23705909 - 10/04/16 07:29 AM (7 years, 3 months ago)

Is it luck? Probably. If it worked for you guys that's awesome. I suppose you just need to get lucky once, then you will have enough LC to do 200 jars but.... the OP had no such luck.

I personally would rather be lucky 90% of the time than 30%. Clean agar with a slight chance to get a contam during transfer as opposed to let me just shoot this syringe in here and hope it doesn't waste my time.:shrug:

<------- less than 5 grows. Drops mic and walks away.:grin:  (sorry, I got cocky lol)


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Edited by PortabellaFella 1 (10/04/16 07:31 AM)


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InvisibleInocuole
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705912 - 10/04/16 07:31 AM (7 years, 3 months ago)

Quote:

PortabellaFella 1 said:
<------- less than 5 grows. Drops mic and walks away.:grin:



Note the bold text..
Quote:

Inocuole said:
Seriously, if you've done less than 5 grows, and you think your sample set is indicative of anything, just shut up




So.. like... this, for instance.

Quote:

shrumzen said:
I made LC from syringes as well and it worked fine for me. Also it should not be the reason for contamination.






:facepalm:  :facepalm3: :judyfacepalm: :doublefacepalm: :epicfacepalm:

https://www.shroomery.org/forums/showflat.php/Number/22721954


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705915 - 10/04/16 07:33 AM (7 years, 3 months ago)

Oh no! I got the smh. I thought I was right.:confused:


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Re: Everything failed! Contaminations! [Re: PortabellaFella 1]
    #23705918 - 10/04/16 07:34 AM (7 years, 3 months ago)

I have no idea what you're talking about... the sentiment was clearly directed toward people who don't understand risk reduction.


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705920 - 10/04/16 07:36 AM (7 years, 3 months ago)

I didn't see the bold text, thanks man :thumbup:


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Re: Everything failed! Contaminations! [Re: thelivingfreekshow]
    #23705950 - 10/04/16 07:52 AM (7 years, 3 months ago)

Quote:

thelivingfreekshow said:
Quote:

pablokabute said:
spores to LC is a bad bad way of germinating and expanding mycelium...




Very true. That being said, I go print>syringe>Lc>Wbs>cvg 66qt mono. I used to use agar, but that got old.




Huh?  I don't think opting for ms because agar got old is logical.  Also, why Would you agree that the above statement is a bad idea(which it is as LC sucks) and then state that is exactly what you do?


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Re: Everything failed! Contaminations! [Re: Mycolorado]
    #23705964 - 10/04/16 08:01 AM (7 years, 3 months ago)

Quote:

Mycolorado said:
Quote:

thelivingfreekshow said:
Quote:

pablokabute said:
spores to LC is a bad bad way of germinating and expanding mycelium...




Very true. That being said, I go print>syringe>Lc>Wbs>cvg 66qt mono. I used to use agar, but that got old.




Huh?  I don't think opting for ms because agar got old is logical.  Also, why Would you agree that the above statement is a bad idea(which it is as LC sucks) and then state that is exactly what you do?




These questions and others are the kind of logical clusterfucks that give rise to my occasional outburst.

:allsodisappointed:

Like, I think if it had made more sense it might've pissed me off less to read it.


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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705999 - 10/04/16 08:33 AM (7 years, 3 months ago)

I honestly don't know how agar gets old. Even if I hated it, make 10 plates, get a culture and clean it up with 3 plates. Then do a small grow. Take 3 clones and transfer once, then a test grow. You should have one plate left at that point. Transfer the best of the 3 clones to that plate, grow it out, inoculate a bunch of LC (50 quarts or so) slant the clone and use the LC for the next 2 years to grow with. Go back to the slant after two years to revive and start more LC. Should take just a few transfers.

20 plates could keep you rolling in vigorous consistent LC that is guaranteed clean for 5-10 years. 20 plates. That's too much :shrug:

Besides anyone who isn't a moron knows that you should be testing your LC periodically and the best thing to test it on is agar. Too much shit can hide in a grain jar and brf is too forgiving of bacteria.

:twocents:


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Re: Everything failed! Contaminations! [Re: Pastywhyte] * 1
    #23706022 - 10/04/16 08:46 AM (7 years, 3 months ago)

the only way agar gets old is if you havent used it correctly...or you havent
used at all. not trying to flame the poster for that but that sounds like

'yah my girl gives great head but meh it gets old' - 30yr old virgin living in parents basement.

agar getting old??????????????? no no no no.


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Re: Everything failed! Contaminations! [Re: mushboy]
    #23706033 - 10/04/16 08:52 AM (7 years, 3 months ago)

Nice analogy. Rated 5 :mushroom:


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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23706383 - 10/04/16 10:52 AM (7 years, 3 months ago)

Quote:

Inocuole said:
Quote:

Mycolorado said:
Quote:

thelivingfreekshow said:
Quote:

pablokabute said:
spores to LC is a bad bad way of germinating and expanding mycelium...




Very true. That being said, I go print>syringe>Lc>Wbs>cvg 66qt mono. I used to use agar, but that got old.




Huh?  I don't think opting for ms because agar got old is logical.  Also, why Would you agree that the above statement is a bad idea(which it is as LC sucks) and then state that is exactly what you do?




These questions and others are the kind of logical clusterfucks that give rise to my occasional outburst.

:allsodisappointed:

Like, I think if it had made more sense it might've pissed me off less to read it.




Just curious, are you agreeing with my question or dissing it?  I thought it made sense.  Maybe I shouldn't have made the parenthesized statement about LC?


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Re: Everything failed! Contaminations! [Re: Mycolorado]
    #23706433 - 10/04/16 11:06 AM (7 years, 3 months ago)

Agar is like an m16
Spores to LC like your uncle's hand in your pants.


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Re: Everything failed! Contaminations! [Re: Mycolorado]
    #23706460 - 10/04/16 11:15 AM (7 years, 3 months ago)

Quote:

Mycolorado said:
Just curious, are you agreeing with my question or dissing it?  I thought it made sense.  Maybe I shouldn't have made the parenthesized statement about LC?




Agreeing that shit is absurd and don't make no sense, yes.


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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23706511 - 10/04/16 11:32 AM (7 years, 3 months ago)

Quote:

Inocuole said:
Quote:

Mycolorado said:
Just curious, are you agreeing with my question or dissing it?  I thought it made sense.  Maybe I shouldn't have made the parenthesized statement about LC?




Agreeing that shit is absurd and don't make no sense, yes.




Right on; some shit on here just makes you scratch your head.  I had to rewrite my original comment a few times as I didn't want to come off like a total prick, but I guess sometimes it's needed warranted.  Thanks, for keeping people in check around here, Inocuole!


Edited by Mycolorado (10/04/16 03:26 PM)


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Re: Everything failed! Contaminations! [Re: Mycolorado]
    #23707134 - 10/04/16 02:50 PM (7 years, 3 months ago)

No its not needed. Op asked why he failed theses things happen. And yes agar plates get old. Injecting anything through a silicone port gets old. Making lc form spores gets old but is still pretty when they go bad color wise i put little chirstmas lights on them. Off topic. Point being is lc is always a pain and is risky, but agar sucks and no offense pasty but only 10 plates at a time no way. The main reason to make plates is to test everything. Making li is just as risky as grain to grain because you can pour contam in. And injecting li is like trying to get blood meal form a fresh bone with your needle. Nothing is ever 100 clean. So let the noobs fail or bloom with there luck. And it is luck not skill if inoculate is bad then all jars will fail with lc. If lc is good even with agar work you still might fail 5 or 6 jars because human error.  Spores to lc isn't the most unsafe way to grow just second most. Fire away. Inucole


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Re: Everything failed! Contaminations! [Re: tump]
    #23707154 - 10/04/16 02:56 PM (7 years, 3 months ago)

lol tump

that poster ment doing agar work got old. like going to work gets old. not the
culture itself gets old.


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Re: Everything failed! Contaminations! [Re: tump]
    #23707158 - 10/04/16 02:57 PM (7 years, 3 months ago)

.


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Edited by amidogen (04/19/18 10:55 AM)


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Re: Everything failed! Contaminations! [Re: amidogen]
    #23707172 - 10/04/16 02:59 PM (7 years, 3 months ago)

growing with a candle next to a waterfall.. duh


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Re: Everything failed! Contaminations! [Re: mushboy]
    #23707191 - 10/04/16 03:03 PM (7 years, 3 months ago)

.


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Edited by amidogen (04/19/18 10:55 AM)


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Re: Everything failed! Contaminations! [Re: tump]
    #23707207 - 10/04/16 03:10 PM (7 years, 3 months ago)

Quote:

tump said:
No its not needed. Op asked why he failed theses things happen. And yes agar plates get old. Injecting anything through a silicone port gets old. Making lc form spores gets old but is still pretty when they go bad color wise i put little chirstmas lights on them. Off topic. Point being is lc is always a pain and is risky, but agar sucks and no offense pasty but only 10 plates at a time no way. The main reason to make plates is to test everything. Making li is just as risky as grain to grain because you can pour contam in. And injecting li is like trying to get blood meal form a fresh bone with your needle. Nothing is ever 100 clean. So let the noobs fail or bloom with there luck. And it is luck not skill if inoculate is bad then all jars will fail with lc. If lc is good even with agar work you still might fail 5 or 6 jars because human error.  Spores to lc isn't the most unsafe way to grow just second most. Fire away. Inucole




God that's incoherent.



I wasn't saying people should be stuck making 10 plates at a time. I was saying that you could make ten plates to do a grow, find a good clone, and expand on a massive scale. You know how people who have government grants grow. Of course most people don't hate agar and want to do loads of it. So for them 10 plates would last one session. But if agar was getting old, 10 might be all you could stand to do.


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Re: Everything failed! Contaminations! [Re: Pastywhyte]
    #23707307 - 10/04/16 03:50 PM (7 years, 3 months ago)

Quote:

Pastywhyte said:
You know how people who have government grants grow.



:burn:


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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


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Offlinetombosley8
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Registered: 10/14/13
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Re: Everything failed! Contaminations! [Re: amidogen]
    #23707356 - 10/04/16 04:11 PM (7 years, 3 months ago)

Quote:

Pastywhyte said:
I honestly don't know how agar gets old. Even if I hated it, make 10 plates, get a culture and clean it up with 3 plates. Then do a small grow. Take 3 clones and transfer once, then a test grow. You should have one plate left at that point. Transfer the best of the 3 clones to that plate, grow it out, inoculate a bunch of LC (50 quarts or so) slant the clone and use the LC for the next 2 years to grow with. Go back to the slant after two years to revive and start more LC. Should take just a few transfers.

20 plates could keep you rolling in vigorous consistent LC that is guaranteed clean for 5-10 years. 20 plates. That's too much :shrug:

:twocents:





I'm sorry but I had to quote for truth.

really simplifies a seemingly overly complicated thing. Love IT!!!!


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OfflineDanktopus
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I'm a teapot

Registered: 10/04/16
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Re: Everything failed! Contaminations! [Re: tombosley8]
    #23718377 - 10/08/16 03:05 AM (7 years, 3 months ago)

Hey, OP here. Thank you all for the outpouring of advice! I am blown away I got this much attention, and I appreciate everything. It got a little heated there but alternative views, discourse, and peer review are all part of the scientific method right? I am just a noob with some grain to grain work in the past, so the LC frontier is new to me. I will definitely be trying out some agar to isolate possible issues because apparently I have them. Sorry I took so long to report back but I wasn't expecting this much response. Here is  a picture of the contamination, its the same with all the jars.



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Everything written here is purely satirical with no basis in reality. All posts are for entertainment purposes only and not meant to be taken seriously. All images are found on the deep web, and all story's and questions are from friends or someone who Isn't me.


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Offlinethelivingfreekshow
Fuck You
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23718458 - 10/08/16 04:52 AM (7 years, 3 months ago)

Quote:

Inocuole said:
Alright... I'm an asshole but I'm not usually this mean... But I've had enough.  Nobody fucking cares how many of you have gotten away with it.  That is so far removed from the entire point.

Think about it for 5 goddamn seconds.  How are spores obtained?  When a fruit is near producing spores, what kind of shit is floating around in the air at the same time?  What's to stop those shits having a field day in your sterilized sugar water?

If you haven't even started fucking with agar you need to put the sugar water away and start understanding what's going on with your cultures.  You probably think everything went fine because you can't even spot bacteria yet.  Seriously, if you've done less than 5 grows, and you think your sample set is indicative of anything, just shut up and save the advice-giving for people who've been around the block enough to fail and figure out why before getting back on the road to success.

I can count on one hand the number of times anybody has benefited from a noob's perspective on LC.  In fact, I could probably still do it if I caught a live hand grenade.




Ive been growing for almost 8 years so don't lecture me, Ive heard it before. I HAVE worked with agar, I prefer not to. How many I get away with  is the point, to me at least. I'm not concerned with your opinion, but you're welcome to it, even if your assumptions on me are baseless. This is what works for me, I never advised op to follow my method, or anyone else, ever, so what are you even talking about? Does it just piss you off because I dont do things how you think they should be done? Too fucking bad.  I am interested in seeing your grenade trick tho, link the video.


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Everything failed! Contaminations! [Re: thelivingfreekshow] * 1
    #23718655 - 10/08/16 08:01 AM (7 years, 3 months ago)

Oh you're one of those people eh? :facepalm:

So if you don't recommend it to people, why the fuck did you bring up your shitty ass nooby af techniques you've been using for 8 years apparently


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OfflineMushierage
SWIM Sinker
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Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
Re: Everything failed! Contaminations! [Re: amidogen]
    #23718737 - 10/08/16 08:51 AM (7 years, 3 months ago)

Quote:

amidogen said:
Quote:

mushboy said:
growing with a candle next to a waterfall.. duh



:laugh2:

Remember that guy that was here a month or two ago who was growing a BRF cake next to a creek at an illegal dumping site for the "ionized FAE source"?




Oh...Fairyflowers...  :lmafo:


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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