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OfflineDanktopus
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Registered: 10/04/16
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Everything failed! Contaminations!
    #23705736 - 10/04/16 04:45 AM (7 years, 3 months ago)

I have recently started an LC operation and I can't seem to get it to work for me. I made 3 different LC jars PC'ed at 15psi for 20 min all with different lid styles trying to figure out which one I like the best. Knocked up with spores and have decent growth in all 3 jars 2 weeks later. I used three different syringes and needles (PC'ed at 15psi for 15 min)  flame sterilized before and after aspirating LC, into 9 different rye berry jars (3 of each LC). My rye berries are added a pinch of gypsum, soaked 24 hours then PC'ed at 15psi for 90 min. Now 3 days after inoculation every single jar has what appears to be the same contamination. My grain lids are self healing ports with synthetic filter discs BTW, and all products have been placed in a brand new incubator set to 77 degrees. Nothing is ever exposed to open air, the injection port I hit heavy with isopropyl befor and after injecting. What am I doing wrong? Please help and any advice is appreciated!
-Danktopus


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Everything written here is purely satirical with no basis in reality. All posts are for entertainment purposes only and not meant to be taken seriously. All images are found on the deep web, and all story's and questions are from friends or someone who Isn't me.


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Offlinethelivingfreekshow
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Re: Everything failed! Contaminations! [Re: Danktopus]
    #23705757 - 10/04/16 05:03 AM (7 years, 3 months ago)

Pics would help.  Also incubators are bad. 65 to 75f is fine for the entire life cycle for cubes. Do you have a lot of experience? Could this contam just be mycelium? Describe it if you cant upload pics.


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: thelivingfreekshow]
    #23705761 - 10/04/16 05:09 AM (7 years, 3 months ago)

I believe the problem is that you inoculated LC with spore syringes. Use agar then transfer clean growth to the LC.


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I would like to acquire anything I don’t have


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Offlinetump
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Re: Everything failed! Contaminations! [Re: PortabellaFella 1]
    #23705767 - 10/04/16 05:22 AM (7 years, 3 months ago)

Bad luck sometimes


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InvisibleTuhdoww
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Registered: 08/23/16
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Re: Everything failed! Contaminations! [Re: tump]
    #23705779 - 10/04/16 05:41 AM (7 years, 3 months ago)

Lc from spores


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Offlineshrumzen
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Registered: 09/07/16
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Re: Everything failed! Contaminations! [Re: PortabellaFella 1]
    #23705796 - 10/04/16 05:59 AM (7 years, 3 months ago)

Quote:

PortabellaFella 1 said:
I believe the problem is that you inoculated LC with spore syringes. Use agar then transfer clean growth to the LC.




I made LC from syringes as well and it worked fine for me. Also it should not be the reason for contamination.

Pictures of the problem would help.


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OfflineGreenskybluegrass7
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Re: Everything failed! Contaminations! [Re: shrumzen]
    #23705808 - 10/04/16 06:06 AM (7 years, 3 months ago)

Quote:

shrumzen said:
Quote:

PortabellaFella 1 said:
I believe the problem is that you inoculated LC with spore syringes. Use agar then transfer clean growth to the LC.




I made LC from syringes as well and it worked fine for me. Also it should not be the reason for contamination.

Pictures of the problem would help.




You just finished your first grow homie


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Only dead fish go with the flow
Fluff not duff


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: shrumzen]
    #23705823 - 10/04/16 06:22 AM (7 years, 3 months ago)

Quote:

shrumzen said:
Quote:

PortabellaFella 1 said:
I believe the problem is that you inoculated LC with spore syringes. Use agar then transfer clean growth to the LC.




I made LC from syringes as well and it worked fine for me. Also it should not be the reason for contamination.

Pictures of the problem would help.




I'm pretty sure you got lucky. The failure rate from MS to LC is huge. All 3 show the same contam, the vector is inside the syringe as he used decent sterile techniques. But that's just from the 1000 posts I've read on the subject. But yes, a pic or two would help as he didn't describe what the contam looks like.


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I would like to acquire anything I don’t have


Edited by PortabellaFella 1 (10/04/16 06:30 AM)


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Invisiblepablokabute
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Re: Everything failed! Contaminations! [Re: PortabellaFella 1]
    #23705842 - 10/04/16 06:41 AM (7 years, 3 months ago)

spores to LC is a bad bad way of germinating and expanding mycelium...


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Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



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--Anonymous Guerilla, or is he..


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Offlinethelivingfreekshow
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Re: Everything failed! Contaminations! [Re: pablokabute]
    #23705890 - 10/04/16 07:13 AM (7 years, 3 months ago)

Quote:

pablokabute said:
spores to LC is a bad bad way of germinating and expanding mycelium...




Very true. That being said, I go print>syringe>Lc>Wbs>cvg 66qt mono. I used to use agar, but that got old. Ive done over 100  jars this year so far sans agar, with maybe 5 or 6 lost to contams . If that's luck, I'll take it.  I try to get the cleanest prints possible, and have honed my sterile technique over several years. Everyone should work with agar at least a few times tho, and most find it very helpful, Im just not one of them. Long story short, its probably your LC, and if youre short on experience, your sterile technique may need work. Pics will most times get you a quicker, more accurate answer.


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InvisibleInocuole
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Re: Everything failed! Contaminations! [Re: thelivingfreekshow]
    #23705900 - 10/04/16 07:22 AM (7 years, 3 months ago)

Alright... I'm an asshole but I'm not usually this mean... But I've had enough.  Nobody fucking cares how many of you have gotten away with it.  That is so far removed from the entire point.

Think about it for 5 goddamn seconds.  How are spores obtained?  When a fruit is near producing spores, what kind of shit is floating around in the air at the same time?  What's to stop those shits having a field day in your sterilized sugar water?

If you haven't even started fucking with agar you need to put the sugar water away and start understanding what's going on with your cultures.  You probably think everything went fine because you can't even spot bacteria yet.  Seriously, if you've done less than 5 grows, and you think your sample set is indicative of anything, just shut up and save the advice-giving for people who've been around the block enough to fail and figure out why before getting back on the road to success.

I can count on one hand the number of times anybody has benefited from a noob's perspective on LC.  In fact, I could probably still do it if I caught a live hand grenade.


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Coir-ca-cola
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InvisibleTuhdoww
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Registered: 08/23/16
Posts: 300
Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705904 - 10/04/16 07:26 AM (7 years, 3 months ago)

Quote:

Inocuole said:
Alright... I'm an asshole but I'm not usually this mean... But I've had enough.  Nobody fucking cares how many of you have gotten away with it.  That is so far removed from the entire point.

Think about it for 5 goddamn seconds.  How are spores obtained?  When a fruit is near producing spores, what kind of shit is floating around in the air at the same time?  What's to stop those shits having a field day in your sterilized sugar water?

If you haven't even started fucking with agar you need to put the sugar water away and start understanding what's going on with your cultures.  You probably think everything went fine because you can't even spot bacteria yet.  Seriously, if you've done less than 5 grows, and you think your sample set is indicative of anything, just shut up and save the advice-giving for people who've been around the block enough to fail and figure out why before getting back on the road to success.

I can count on one hand the number of times anybody has benefited from a noob's perspective on LC.  In fact, I could probably still do it if I caught a live hand grenade.




I been waiting for you noc:rofl:


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: thelivingfreekshow]
    #23705909 - 10/04/16 07:29 AM (7 years, 3 months ago)

Is it luck? Probably. If it worked for you guys that's awesome. I suppose you just need to get lucky once, then you will have enough LC to do 200 jars but.... the OP had no such luck.

I personally would rather be lucky 90% of the time than 30%. Clean agar with a slight chance to get a contam during transfer as opposed to let me just shoot this syringe in here and hope it doesn't waste my time.:shrug:

<------- less than 5 grows. Drops mic and walks away.:grin:  (sorry, I got cocky lol)


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I would like to acquire anything I don’t have


Edited by PortabellaFella 1 (10/04/16 07:31 AM)


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InvisibleInocuole
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705912 - 10/04/16 07:31 AM (7 years, 3 months ago)

Quote:

PortabellaFella 1 said:
<------- less than 5 grows. Drops mic and walks away.:grin:



Note the bold text..
Quote:

Inocuole said:
Seriously, if you've done less than 5 grows, and you think your sample set is indicative of anything, just shut up




So.. like... this, for instance.

Quote:

shrumzen said:
I made LC from syringes as well and it worked fine for me. Also it should not be the reason for contamination.






:facepalm:  :facepalm3: :judyfacepalm: :doublefacepalm: :epicfacepalm:

https://www.shroomery.org/forums/showflat.php/Number/22721954


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705915 - 10/04/16 07:33 AM (7 years, 3 months ago)

Oh no! I got the smh. I thought I was right.:confused:


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I would like to acquire anything I don’t have


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InvisibleInocuole
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Re: Everything failed! Contaminations! [Re: PortabellaFella 1]
    #23705918 - 10/04/16 07:34 AM (7 years, 3 months ago)

I have no idea what you're talking about... the sentiment was clearly directed toward people who don't understand risk reduction.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflinePortabellaFella 1
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705920 - 10/04/16 07:36 AM (7 years, 3 months ago)

I didn't see the bold text, thanks man :thumbup:


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I would like to acquire anything I don’t have


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InvisibleMycolorado
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Re: Everything failed! Contaminations! [Re: thelivingfreekshow]
    #23705950 - 10/04/16 07:52 AM (7 years, 3 months ago)

Quote:

thelivingfreekshow said:
Quote:

pablokabute said:
spores to LC is a bad bad way of germinating and expanding mycelium...




Very true. That being said, I go print>syringe>Lc>Wbs>cvg 66qt mono. I used to use agar, but that got old.




Huh?  I don't think opting for ms because agar got old is logical.  Also, why Would you agree that the above statement is a bad idea(which it is as LC sucks) and then state that is exactly what you do?


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InvisibleInocuole
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Re: Everything failed! Contaminations! [Re: Mycolorado]
    #23705964 - 10/04/16 08:01 AM (7 years, 3 months ago)

Quote:

Mycolorado said:
Quote:

thelivingfreekshow said:
Quote:

pablokabute said:
spores to LC is a bad bad way of germinating and expanding mycelium...




Very true. That being said, I go print>syringe>Lc>Wbs>cvg 66qt mono. I used to use agar, but that got old.




Huh?  I don't think opting for ms because agar got old is logical.  Also, why Would you agree that the above statement is a bad idea(which it is as LC sucks) and then state that is exactly what you do?




These questions and others are the kind of logical clusterfucks that give rise to my occasional outburst.

:allsodisappointed:

Like, I think if it had made more sense it might've pissed me off less to read it.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisiblePastywhyteMDiscord
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Re: Everything failed! Contaminations! [Re: Inocuole]
    #23705999 - 10/04/16 08:33 AM (7 years, 3 months ago)

I honestly don't know how agar gets old. Even if I hated it, make 10 plates, get a culture and clean it up with 3 plates. Then do a small grow. Take 3 clones and transfer once, then a test grow. You should have one plate left at that point. Transfer the best of the 3 clones to that plate, grow it out, inoculate a bunch of LC (50 quarts or so) slant the clone and use the LC for the next 2 years to grow with. Go back to the slant after two years to revive and start more LC. Should take just a few transfers.

20 plates could keep you rolling in vigorous consistent LC that is guaranteed clean for 5-10 years. 20 plates. That's too much :shrug:

Besides anyone who isn't a moron knows that you should be testing your LC periodically and the best thing to test it on is agar. Too much shit can hide in a grain jar and brf is too forgiving of bacteria.

:twocents:


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