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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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g2g in mycosacs
#23704962 - 10/03/16 08:37 PM (7 years, 3 months ago) |
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hello friends, i am getting ready to do a bunch of g2g transfers, and it seems very similar to the process for making lions mane blocks (tyvek sleeve inside)
my lions mane blocks were made using bags from fungi perfecti. Recently, i got some mycosacs from aloha to try out, and they are a lot different than the bags ive used in the past

i already emailed aloha but got no reply: is the g2g process any different for these bags? do i still need to use tyvek sleeves in each bag? also is there any alternative to the tyvek sleeves?
thanks guys!
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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No you doing the same. But if its loin mane you probaby want to ask in medical and gormen forum
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 10 months, 42 minutes
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DELETED. SEE POST BELOW.
Edited by RyeJar (10/04/16 12:22 AM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Quote:
c10h12n2o said: hello friends, i am getting ready to do a bunch of g2g transfers, and it seems very similar to the process for making lions mane blocks (tyvek sleeve inside)
my lions mane blocks were made using bags from fungi perfecti. Recently, i got some mycosacs from aloha to try out, and they are a lot different than the bags ive used in the past

i already emailed aloha but got no reply: is the g2g process any different for these bags? do i still need to use tyvek sleeves in each bag? also is there any alternative to the tyvek sleeves?
thanks guys!
Don't it however you did the other bags. I personally don't use sleeves. Sweet bags btw. I've been wanting to try em out for a while now. Let me know if they're worth double the cost of the other type.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: g2g in mycosacs [Re: azur]
#23705074 - 10/03/16 09:21 PM (7 years, 3 months ago) |
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much obliged my friends 
i meant that i had done lions mane in the past this way, and now have grain masters of lots of stuff ready to go, and wanted to check if the weird filter patches made any difference as to what needs to be done
Quote:
RyeJar said: Yeah it is called an autoclave. I personally recommend them but everyone else will disagree.
thanks for the advice!
do you mind clarifying a bit what you mean though? i am probably missing something, but how does the autoclave serve as an alternative to tyvek sleeves? also, why exactly do other people disagree, and why do you recommend them vs an AA PC?
Quote:
azur said: Don't it however you did the other bags. I personally don't use sleeves. Sweet bags btw. I've been wanting to try em out for a while now. Let me know if they're worth double the cost of the other type.
will do buddy! i burned right through them on my first time with my impulse sealer, definitely a lot different than the FP bags (more clear, thinner, but seem to PC well, id expect fewer micro holes in the folds)
this is what i wanted to hear ... how exactly do you do em without sleeves? just fold accordion style and rubber band them over, then open them in the SAB/flowhood?
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 10 months, 42 minutes
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DELETED. SEE POST BELOW.
Edited by RyeJar (10/04/16 12:22 AM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: g2g in mycosacs [Re: RyeJar]
#23705094 - 10/03/16 09:30 PM (7 years, 3 months ago) |
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seriously?? the only reason people advise against autoclaves is price? if thats the case i will pick one up pronto.
ive seen some pretty sweet ones, even AA makes some. is there any other fundamental reason not to use an autoclave vs a PC? or any particular task that a an autoclave might be less called for than a PC (besides cooking)?
thanks again!
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 10 months, 42 minutes
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DELETED. SEE POST BELOW.
Edited by RyeJar (10/04/16 12:22 AM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I fold the top down from the grain up, then fold the very top back under. No sleeve, taken or rubber band. Use other bags to hold them folded
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: g2g in mycosacs [Re: azur]
#23705138 - 10/03/16 09:45 PM (7 years, 3 months ago) |
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lol thats awesome, thanks buddy, i will be picking one up this week 
btw i have been doing something lately to try to reduce risk of contaminated air being sucked into my agar, does this sound like it would work? I have been making a point to vent the pc at slightly above 0 , since the positive pressure should prevent it from sucking anything in. before, i had been wrapping an alcohol-soaked rag around the vent, but seems like positive pressure might be more effective
Quote:
azur said: I fold the top down from the grain up, then fold the very top back under. No sleeve, taken or rubber band. Use other bags to hold them folded
excellent, thanks for clarifying, i will be trying that tomorrow for sure
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 10 months, 42 minutes
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Deleted all posts in this thread due to misinformation. Sorry. I fell off the alcohol wagon last night. Just forget I posted in this thread at all.
Edited by RyeJar (10/04/16 12:17 AM)
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: g2g in mycosacs [Re: RyeJar]
#23705472 - 10/03/16 11:37 PM (7 years, 3 months ago) |
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Cool bags! I've been using tyvek sleeves because I thought they helped prevent blowout. Also with .2 micron bags they protect the filter, but maybe the tyvek isn't necessary? I'll have to try some runs without it. I fold my bags shut the same way azur described and then close them with painter's tape, but my autoclave isn't big enough to stack bags. I was always under the impression that the tyvek held the bags partially open so steam could escape without blowing the bag open.
And yeah, when I do bags I let the autoclave cool completely, which creates a vacuum inside, and I open the valve in front of the flowhood with a clorox wipe wrapped around it.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 10 months, 42 minutes
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Re: g2g in mycosacs [Re: RyeJar]
#23705542 - 10/04/16 12:24 AM (7 years, 3 months ago) |
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Quote:
c10h12n2o said: lol thats awesome, thanks buddy, i will be picking one up this week 
btw i have been doing something lately to try to reduce risk of contaminated air being sucked into my agar, does this sound like it would work? I have been making a point to vent the pc at slightly above 0 , since the positive pressure should prevent it from sucking anything in. before, i had been wrapping an alcohol-soaked rag around the vent, but seems like positive pressure might be more effective
Quote:
azur said: I fold the top down from the grain up, then fold the very top back under. No sleeve, taken or rubber band. Use other bags to hold them folded
excellent, thanks for clarifying, i will be trying that tomorrow for sure
Quote:
RyeJar said: Deleted all posts in this thread due to misinformation. Sorry. I fell off the alcohol wagon last night. Just forget I posted in this thread at all.
Just get a PC and listen to azur. Ughh, that makes me feel dirty.
Oh, love you azur .
Edited by RyeJar (10/04/16 12:40 AM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: g2g in mycosacs [Re: RyeJar]
#23708180 - 10/04/16 09:10 PM (7 years, 3 months ago) |
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