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sovietsyn
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Registered: 03/22/13
Posts: 18
Last seen: 7 years, 3 months
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First Innoc. w/Liquid Culture...Is this mycellium? [Pics]
#23702246 - 10/03/16 01:28 AM (7 years, 3 months ago) |
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I knocked these up on the 26th, and a few on the 28th. Showing the most growth ones. I used a Karo solution to do my LC. I made my LC from a B+ Spore syringe. I am not sure this is mycellium, it is very much a clumpy, non rhizo growth - though this past 24 hours I noticed some "strands" forming at the ends of these clumpy/non rhizo type growths.
What do you guys think?
Being this is my first LC I might be taking it too serious, hoping to see intense rhizo, as I have from some of my MS.
Anyway...is this P. cubensis myc or am I growing something else?
Sorry guys...I tend to over analyze, and am a bit "green"/new.
Thanks!
Test Subjects "SLQ01":

Half Pints:



The 1/4 Pint:
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: sovietsyn]
#23702378 - 10/03/16 03:31 AM (7 years, 3 months ago) |
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Quote:
sovietsyn said: I used a Karo solution to do my LC. I made my LC from a B+ Spore syringe.
Stopped reading here... This is not how you make a clean LC. I wouldn't have any faith in a LC made this way at all. If you aren't starting with agar, and are shooting a syringe of spores into sugar water, you're playing with fire. Bacteria loves sugar water, and spores and bacteria tend to live in the same environment for a while before the spores are collected so... Disaster is more or less imminent.
The growth in the jars already looks a little iffy to me.
Why use LC to inoculate PF jars anyway? LC is for creating huge amounts of inoculate. Not.. the amount you would use for PF tek.
LC for PF tek is like using a bulldozer to pick up dog shit from your yard. PF tek is made for shooting spores into. If you change that up and use infected sugar water instead, you can expect difficulty.
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sovietsyn
Zing


Registered: 03/22/13
Posts: 18
Last seen: 7 years, 3 months
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: Inocuole]
#23702411 - 10/03/16 04:06 AM (7 years, 3 months ago) |
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I plan to in the future move on from PF Tek (I already have bgeun) , but being I had two syringes - I made 1 LC from each syringe - also 11 jars from each, ending up with two LC's/22 jars. This just seems the most economical and also educational way, since I can step up to agar in due time...I read some teks here saying I would be fine with karo tek LC...I have self healing inoculation ports as well as another port for vacuum equalization/GE...sorry as I am new here, I may have read old tek. But I am just wondering if what is seen is myc, as I wasn't sure until I saw the strands recently...anyway...time for bed. Also I am new, so forgive me.
I have posted pictures for review, I know I am new, but just wish to know if I should toss these jars or leave them in isolation. Thank you!
edit: What would be the best way to move on from MS/Spore Syringes into LC, and eventually full on agar teks? I have read various cloning and LC teks citing use of such things as Karo and splitting a fresh fruit in half to "spear it" with a syringe, then injecting into rye or a nutrient rich fluid (Karo or similair) so I am just confused a bit, been out of the loop lately and also just getting mis matched with all the diff teks around :/
Edited by sovietsyn (10/03/16 04:36 AM)
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: sovietsyn]
#23702649 - 10/03/16 07:39 AM (7 years, 3 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:50 AM)
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: sovietsyn]
#23702799 - 10/03/16 08:54 AM (7 years, 3 months ago) |
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Quote:
Inocuole said:
Quote:
sovietsyn said: I used a Karo solution to do my LC. I made my LC from a B+ Spore syringe.
Stopped reading here... This is not how you make a clean LC. I wouldn't have any faith in a LC made this way at all. If you aren't starting with agar, and are shooting a syringe of spores into sugar water, you're playing with fire. Bacteria loves sugar water, and spores and bacteria tend to live in the same environment for a while before the spores are collected so... Disaster is more or less imminent.
The growth in the jars already looks a little iffy to me.
Why use LC to inoculate PF jars anyway? LC is for creating huge amounts of inoculate. Not.. the amount you would use for PF tek.
LC for PF tek is like using a bulldozer to pick up dog shit from your yard. PF tek is made for shooting spores into. If you change that up and use infected sugar water instead, you can expect difficulty.
Lol, this^
Quote:
sovietsyn said: I plan to in the future move on from PF Tek (I already have bgeun) , but being I had two syringes - I made 1 LC from each syringe - also 11 jars from each, ending up with two LC's/22 jars. This just seems the most economical and also educational way, since I can step up to agar in due time...I read some teks here saying I would be fine with karo tek LC...I have self healing inoculation ports as well as another port for vacuum equalization/GE...sorry as I am new here, I may have read old tek. But I am just wondering if what is seen is myc, as I wasn't sure until I saw the strands recently...anyway...time for bed. Also I am new, so forgive me.
I have posted pictures for review, I know I am new, but just wish to know if I should toss these jars or leave them in isolation. Thank you!
edit: What would be the best way to move on from MS/Spore Syringes into LC, and eventually full on agar teks? I have read various cloning and LC teks citing use of such things as Karo and splitting a fresh fruit in half to "spear it" with a syringe, then injecting into rye or a nutrient rich fluid (Karo or similair) so I am just confused a bit, been out of the loop lately and also just getting mis matched with all the diff teks around :/
Why is everyone so afraid of agar? They should be afraid not to use it. I don't get it, it is one of the simplest methods there is and a proper LC is the most advanced method IMO. If you can make jello you can make agar, I guarantee I could teach my 8 year old to make and use agar, helping with the PC of course. It's like "I'm afraid to drop spores onto a 2d surface where I can easily see and transfer away from contams so I'm going to put spores in a liquid where I can't tell if it's contaminated or not" To create a proper LC one would need to clean a culture up on agar, use a wedge to inoculate the LC and then test the LC. And unless you are nocing up several dozens of grain jars at a time I wouldn't even bother with LC, you're not saving any time and just adding another vector.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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sovietsyn
Zing


Registered: 03/22/13
Posts: 18
Last seen: 7 years, 3 months
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: NDStepp84]
#23703310 - 10/03/16 11:42 AM (7 years, 3 months ago) |
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Thank you guys, looks like I have some reading to do and some supplies to get next round.
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: sovietsyn]
#23703643 - 10/03/16 01:30 PM (7 years, 3 months ago) |
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For cakes just use a syringe, but for when you move on to bulk check out pastywhyte's agar tek in the links/teks in my signature, you can get started for around 20$ from stuff sourced locally. Other than the agar bars in the tek many use telephone brand agar powder, about 2$ a pack and enough for about 100 plates
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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sovietsyn
Zing


Registered: 03/22/13
Posts: 18
Last seen: 7 years, 3 months
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: NDStepp84]
#23708623 - 10/05/16 12:15 AM (7 years, 3 months ago) |
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Good news is I have a viable LC it seems, as I don't seem to have any contam jars. I already used all of my syringe and haven't finished my first flush yet so I have no print to make a new syringe. But I did start a bunch of new jars using the LC. Only thing I noticed is it is not very rhizo at first, then it gets strands later. I will be looking into agar soon as I find it will be very fun to isolate different "strains" characteristics.
Thanks again
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: sovietsyn]
#23708912 - 10/05/16 05:10 AM (7 years, 3 months ago) |
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Oh the LC is viable because jars don't seem contam'd? Darn, I wish I had known that was all it took before I went years thinking you had to test your LC on agar..
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: Inocuole]
#23708931 - 10/05/16 05:24 AM (7 years, 3 months ago) |
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Dam inocule. Chill this topic really pisses you off. First op welcome . second that does look like myc but a litte bacteria with the fluffy look. Now i will agree that a clean lc makes the world grow so much better and the fruit better in the end. On the other end if you make clean lc and still like cake making more power to you. Cakes are very forgiving compared to making perfect grain spawn. Once you have clean cultures you probably won't want to go back. Start with agar now making clean plates can take month's.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: tump]
#23708933 - 10/05/16 05:26 AM (7 years, 3 months ago) |
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Oh okay, if only we could all be as patient as government grant boy..
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: Inocuole]
#23708950 - 10/05/16 05:44 AM (7 years, 3 months ago) |
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Yes patient. You know one of those things you need to grow anything including mushrooms. The very best teacher in life is failure. You can couch people and give info all you want. But if your snippy with noobs they will take it personal. Then blow you off until they fail a lot. We have told him about agar and proper ways for lc use. I know this is the third one of these spores to lc threads in a day but ease up on the noobs. They get there info form looking up lc on google. You can't blame them for using old info.
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sovietsyn
Zing


Registered: 03/22/13
Posts: 18
Last seen: 7 years, 3 months
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Re: First Innoc. w/Liquid Culture...Is this mycellium? [Pics] [Re: tump]
#23709941 - 10/05/16 01:10 PM (7 years, 3 months ago) |
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I am just experimenting around...sorry to offend anyone.
I'll start looking into agar over the next two weeks while these flushes finish.
Edited by sovietsyn (10/05/16 01:13 PM)
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