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Offlinefunguy14
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Unknown death of shitake spores in LC
    #23697107 - 10/01/16 12:34 PM (7 years, 4 months ago)

I recently brought some shitake cultures down to Texas during the heat of the summer and didn't think about the threshold for survival of the spores. I followed an experimental research method using bird seed with the BRF in my PF Tek and knocked up 5 half pint jars with zero mycellial growth. I am rather experienced at research and have had much success with other experiments in the past,, however, I was surprised to see that this particular experiment didn't take off. Has anyone out there had similar experiences? I also noticed that the spores had germinated in the LC when i got them in the mail and I put them in the fridge right away. I have seen other posts of similar concern and learned from reading on here that sometimes this does help slow them down. I'm discouraged to say the least.


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Offlinekatbusa
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Re: Unknown death of shitake spores in LC [Re: funguy14]
    #23697244 - 10/01/16 01:32 PM (7 years, 4 months ago)

What temperatures were they exposed to? How long did you wait before you called it quits?

Edited by katbusa (10/01/16 01:37 PM)

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OfflineGreg
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Re: Unknown death of shitake spores in LC [Re: katbusa]
    #23697856 - 10/01/16 05:44 PM (7 years, 4 months ago)

You mention cultures and then you mention spores?
Which is it? One or the other.

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Offlinekatbusa
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Re: Unknown death of shitake spores in LC [Re: Greg]
    #23698366 - 10/01/16 08:47 PM (7 years, 4 months ago)

I have a feeling it was a culture. I might be wrong but I haven't heard of spores germinating in a spore syringe. If I remember correctly sterilized water is used to suck up spores from a print. No nutrient solution.

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Offlinefunguy14
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Re: Unknown death of shitake spores in LC [Re: katbusa]
    #23700935 - 10/02/16 04:59 PM (7 years, 4 months ago)

It was a LC syringe, and they were exposed to 110 degree heat or more during the mailing process. And in spite of other comments, I have done alot of research on the germination of spores inside a syringe and they can take on germination if they get to hot. I knocked up 5 half pint jars and let them go for two weeks before I called it quits. One took on green tric and I threw it away as soon as I caught it. I have a black out sterilite tote for incubation and I keep therms inside and outside the tote to monitor the temps. I have been doing this for two years now and the shitake is the first one to ever give me problems.


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Offlinefunguy14
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Re: Unknown death of shitake spores in LC [Re: Greg]
    #23700947 - 10/02/16 05:02 PM (7 years, 4 months ago)

I said LC meaning LIQUID CULTURE. Which contain SPORES there smart one. Know what your talking about before you get on here and flip someone guff. Farm on bro!!!


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OfflineGreg
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Re: Unknown death of shitake spores in LC [Re: funguy14]
    #23700969 - 10/02/16 05:09 PM (7 years, 4 months ago)

Liquid culture is living tissue in liquid nutrient medium.
Liquid culture typically does not contain spores.
If your liquid culture has spores in it, you're doing something wrong.

Don't take offense to a valid question.

What's with your sporadic capitalization anyway?

Edited by Greg (10/02/16 05:17 PM)

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Offlinefunguy14
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Re: Unknown death of shitake spores in LC [Re: Greg]
    #23700991 - 10/02/16 05:16 PM (7 years, 4 months ago)

and the butt hurt just keeps on coming. You're flaming there smart one. And I capitalize so good more educated ones like your self can see my emotion. It's an age old grammar tool. Some of us still use it. And YES without SPORES, mushrooms can not grow. so you are wrong again!!!


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OfflineGreg
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Re: Unknown death of shitake spores in LC [Re: funguy14]
    #23700994 - 10/02/16 05:17 PM (7 years, 4 months ago)

:archiebunker:

It's not worth arguing with you.
Have fun with your mystery shiitake spore LC.

Edited by Greg (10/02/16 05:27 PM)

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Offlinefunguy14
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Re: Unknown death of shitake spores in LC [Re: Greg]
    #23701034 - 10/02/16 05:28 PM (7 years, 4 months ago)

Furthermore and for your own closure to the subject. I don't PRINT. I order my LC from a sponsor. So thank you for the education and if you have something that is going to help me move forward in my adventure then I would be glad to hear from you and take any useful advice that you may have. I'm not much into competing with people over who is smarter or has better grammar. I'm pretty well read into the subject myself and though I may hold different opinions into what a LC may or may not be, without the sperm, the baby is not possible. so your scientific tissue argument is invalid anyway, because nothing is made possible without the spore first, that is the step that starts it. Go buy yourself a few books from some published guys that have masters degrees in this area. They are very helpful. I have a few myself.


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Offlinekatbusa
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Re: Unknown death of shitake spores in LC [Re: funguy14]
    #23701039 - 10/02/16 05:29 PM (7 years, 4 months ago)

Calm down man. LC and spore syringe are two different things. I persoanlly have never seen spores germinate inside of a syringe because mine do not stick around long at all. Aparently its possible.

Good luck with the Trich.

Edited by katbusa (10/02/16 05:30 PM)

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Offlinefunguy14
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Re: Unknown death of shitake spores in LC [Re: katbusa]
    #23701066 - 10/02/16 05:40 PM (7 years, 4 months ago)

I have heard that temps above 106 can kill shitake because they typically like cooler weather. I was just wondering if anyone else had experienced the same problem in the past. I work a full time job so I put the syringes in the fridge for 4 days before use but they had already shown activity when I inspected them. I have in the past had cubes try to do it on me, because I left them at room temp, but that was while I was up in Alaska and it didn't get as hot. I was just surprised to see that after innoculating my jars that they were pretty much sterile at that point. They didn't give me any babies.


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OfflineQuadman
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Re: Unknown death of shitake spores in LC [Re: funguy14]
    #23701644 - 10/02/16 09:02 PM (7 years, 4 months ago)

A very old book has this to say: Don't answer the foolish arguments of fools, or you will become as foolish as they are.

Now some advice, if you ask for help be sincere and humble it will go much further than being self righteous.
I suggest funguy14 look up tissue culture and read about it. You will then understand the conversation. Tissue culture applies to both plants and fungi.


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