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ChicagoMan
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Identify contam Plz, if trich maybe still make tea? ***Update consensus fruits should be gtg
#23697648 - 10/01/16 04:20 PM (7 years, 3 months ago) |
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So close but not quite... Did cakes, trays, and a few Mono's. This is first mono from grains. Never had a contam before but got my ass handed to me this round. This is the first flush, it colonized the coir/verm/coffee/gypsum Very fast, 5 to 6 days but had spots from day 3 on. Should have dumped them?...yes I'm sure...is that still the best and possibly only idea, probably? Please let me know what you think contamination is and if safe to eat. Thanks much!
Edited by ChicagoMan (10/02/16 05:04 AM)
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morty422
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23697675 - 10/01/16 04:28 PM (7 years, 3 months ago) |
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The fruits are fine to consume as long as there is no visible mold growth found on them.
As far as identification of contaminate, you will have to wait for someone else who is more educated.
I would make sure this is in an area other than your current grow room-as you don't want to spread the nasties around, possibly contaminating future grows.
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dankington
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Re: Identify contam Plz, if trich maybe still make tea? [Re: morty422]
#23697686 - 10/01/16 04:32 PM (7 years, 3 months ago) |
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What in the fuck is going on in the first pic? It looks like you cut out a big chunk and poured milk into it. Then the sides started getting moldy anyways.

But the fruits should be okay.
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Greg
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Re: Identify contam Plz, if trich maybe still make tea? [Re: dankington]
#23697701 - 10/01/16 04:37 PM (7 years, 3 months ago) |
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 What the hell is that all about?
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: morty422]
#23697705 - 10/01/16 04:39 PM (7 years, 3 months ago) |
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Quote:
morty422 said: The fruits are fine to consume as long as there is no visible mold growth found on them.
As far as identification of contaminate, you will have to wait for someone else who is more educated.
I would make sure this is in an area other than your current grow room-as you don't want to spread the nasties around, possibly contaminating future grows.

There is a visable growth on them if you looked at the pics. Maybe its just odd mycelium. I havent seen anything quite like that before. The first pick is gross. I would just toss that anyways and clean the shit out of your lab before trying a new batch. What is that goop?!?! Its hard to see from the pics but is that a thick layer of mold on your substrate where you cut it out?
Edited by RyeJar (10/01/16 04:41 PM)
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Greg]
#23697711 - 10/01/16 04:42 PM (7 years, 3 months ago) |
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I did cut a big chunk out of the 1 tub and salted the shit out of the surrounding area to stop green growth, worked for a while...then??? All the PE opened their caps as soon as they popped up, very mutated mutants!!
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23697716 - 10/01/16 04:44 PM (7 years, 3 months ago) |
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I would just be sure to double check your sterile technique and pasteurization methods and make sure your grains are contam free before using them to spawn. Again, I would scrub your area down, get the contaminated substrate out of the area without disturbing the spores too much.
Edited by RyeJar (10/01/16 04:45 PM)
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23697728 - 10/01/16 04:51 PM (7 years, 3 months ago) |
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No doubt about some scrubbing entire room, hell maybe I'll take the wife and cat out and bleach bomb it. And any picking will be done outside and then inhailated... Jars sat after going through PC for about a week before innoculating, made my own dirty spore syringe from injecting water into upside down giant (85g) PE cap, massaging cap then extracting water with syringe. Got a pretty decently dark spore syringe...but who cares because it was contaminated. I lost 4 out of 12 jars before spawning, I knew I could very well be in trouble, but at least I know where it happened
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23697737 - 10/01/16 04:55 PM (7 years, 3 months ago) |
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Went from this
 To this

Damn
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dankington
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23697750 - 10/01/16 05:01 PM (7 years, 3 months ago) |
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I think that's just the unstable genetics of PE, that doesn't look like contam to me, necessarily. PE puts out some funky fruits.
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23697752 - 10/01/16 05:02 PM (7 years, 3 months ago) |
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Quote:
ChicagoMan said: No doubt about some scrubbing entire room, hell maybe I'll take the wife and cat out and bleach bomb it. And any picking will be done outside and then inhailated... Jars sat after going through PC for about a week before innoculating, made my own dirty spore syringe from injecting water into upside down giant (85g) PE cap, massaging cap then extracting water with syringe. Got a pretty decently dark spore syringe...but who cares because it was contaminated. I lost 4 out of 12 jars before spawning, I knew I could very well be in trouble, but at least I know where it happened
Yeah that syringe method is a big no IMO. For it to have worked well, you needed to put those spores on agar to clean them up. If I start with spores I will inject them onto grain and when colonized I will take single grains and pop em on a few plates of agar and then I will use the rest to spawn to a bulk sub just to get a few babies out of em. The real work is cleaning them up on the agar or if you are serious clean them up and isolate a few strains from them.
Edited by RyeJar (10/01/16 05:04 PM)
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23697838 - 10/01/16 05:38 PM (7 years, 3 months ago) |
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Yes sir, doing some Agar work now, and have 8 jars colonizing from clean Agar wedge just dropped in, in a SAB...flow hood to come soon.
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23697867 - 10/01/16 05:47 PM (7 years, 3 months ago) |
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Quote:
ChicagoMan said: Yes sir, doing some Agar work now, and have 8 jars colonizing from clean Agar wedge just dropped in, in a SAB...flow hood to come soon.
Dont worry about flowhoods. They are nice for some things but there are a few things you would do more harm than good by using one like cleaning up dirty plates. I think a better use for the money is an AA Autoclave. Best money I have ever spent. Got my first one for 50 bux at a "goodwill" 10 years ago and just got brand new but open box for 150 dollars on ebay.
Edited by RyeJar (10/01/16 05:49 PM)
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Greg
always learning




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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23697869 - 10/01/16 05:49 PM (7 years, 3 months ago) |
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Unless you're handling a very large volume of spawn or something, a SAB is your best bet.
If you've got money burning a hole in your pocket definitely get an AA pressure cooker. It's a true game changer.
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Greg]
#23697873 - 10/01/16 05:50 PM (7 years, 3 months ago) |
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Quote:
Greg said:

Unless you're handling a very large volume of spawn or something, a SAB is your best bet.
If you've got money burning a hole in your pocket definitely get an AA pressure cooker. It's a true game changer.
Keep the pressure cookers for food use. Get the autoclave for tissue culture/lab work (my personal opinion of course.)
Edited by RyeJar (10/01/16 05:52 PM)
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Greg
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23697879 - 10/01/16 05:53 PM (7 years, 3 months ago) |
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The biggest AA canner is about 2x less expensive than the smallest AA autoclave though, and for our purposes works just as well...
Really the only important things are that it gets to at least 15 PSI and holds a lot of jars/bags. Which either the autoclave or canner does.
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Greg]
#23697885 - 10/01/16 05:55 PM (7 years, 3 months ago) |
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It does work just as well with certain precautions but I got a 25qt for 150 never used. If that wasn't worth the money then I dont know what is.
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Greg
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23697888 - 10/01/16 05:56 PM (7 years, 3 months ago) |
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Damn that's a good price. Nice find.
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Greg]
#23697889 - 10/01/16 05:57 PM (7 years, 3 months ago) |
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Quote:
Greg said: Damn that's a good price. Nice find.
I was giddy.
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dankington
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23697907 - 10/01/16 06:03 PM (7 years, 3 months ago) |
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my presto 16 or whatever is pretty cool…
Though using my buddy's 941 is pretty awesome
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: dankington]
#23697925 - 10/01/16 06:08 PM (7 years, 3 months ago) |
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Quote:
dankington said:
my presto 16 or whatever is pretty cool…
Though using my buddy's 941 is pretty awesome 
I was rocking that presto inbetween the periods I lost my first autoclave and got my new one. It worked nice. Didn't have too many complaints but I never did any technical tissue work with it either.
I was always put off with the fact that cookers sucks in dirty air as they cool. Causes you to take filtering precautions with everything you put into it.
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dankington
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23697934 - 10/01/16 06:11 PM (7 years, 3 months ago) |
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yeah, but you work with what you can. Without it, I'd still be failing miserably with cakes. Now I just fail moderately 
But seriously, the PC is fine too. Sterilizers are preferred, but we can work well with a PC.
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freespeech
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Re: Identify contam Plz, if trich maybe still make tea? [Re: dankington]
#23698008 - 10/01/16 06:37 PM (7 years, 3 months ago) |
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If you want a better chance of success, go with a mainstream strain over PE. It's more difficult to grow PE.
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: freespeech]
#23698061 - 10/01/16 06:52 PM (7 years, 3 months ago) |
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Everything has gone through my 16qt presto PC, couldn't justify 3x more $ on the AA, I'd love one, I'm a Chem E, and autoclave medical instruments for cadaver use in my lab. So agreement with wife is if and when presto fails, I get to buy any AA I want....kinda a prove ur going use it senerio. They do the same thing, arguably just as well, 15psi is 15 psi...but the AA looks like a piece of lab equipment and last 3 lifetimes!!!
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23698077 - 10/01/16 06:57 PM (7 years, 3 months ago) |
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Quote:
ChicagoMan said: Everything has gone through my 16qt presto PC, couldn't justify 3x more $ on the AA, I'd love one, I'm a Chem E, and autoclave medical instruments for cadaver use in my lab. So agreement with wife is if and when presto fails, I get to buy any AA I want....kinda a prove ur going use it senerio. They do the same thing, arguably just as well, 15psi is 15 psi...but the AA looks like a piece of lab equipment and last 3 lifetimes!!!
I just googled shopped 15qt aa pressure cooker. They start at 210. They are selling a 25 qt aa autoclave on ebay brand new for 250. Where is this 2x and 3x the price coming from? Again, with patience I found my 25 qt for 150.
Edit: Just realized you meant 3x the price of the presto. But the difference in price between an aa cooker and autoclave is negligible under the right conditions.
Edited by RyeJar (10/01/16 07:01 PM)
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23698100 - 10/01/16 07:05 PM (7 years, 3 months ago) |
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I have had great success startingall this just back in March with 2 syringes, one Treasure Coast, the other PE. Many less fruits with PE but total dry weights were almost the same. Given the higher potential potency, I'm sticking with it. I do still have multiple spore prints from the TC that I can utilize at some point. Looking at getting grain prep done correctly and no contams in any jars before spawning. Testing small then doing probably 5 66qt PE Mono's and maybe 5 of the TC. Just need to get my Agar clean and isolated, and I have learned a lot about getting my moisture content perfect for substrate and for the grain jars. Experience is obviously the key! Best cleanest route?....spore/tissue to Agar, Agar to grain master, grain jars spawned to substrate...many opinions I know, but let me know your favored methods, I've read litterly thousands of ways so again let me know if you have a personal favorite. Also Agar to grain master, just use a wedge, or make Agar into Liquid innoculating? Thanks for all replies!
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23698110 - 10/01/16 07:09 PM (7 years, 3 months ago) |
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I was given a 6qt electric PC from mother-in-law, it was worth like $85 on a return for store credit, picked up the presto for $79. Once I have 10 perfect Mono's, I'll be able to justify any piece of equipment I would like, possibly a real autoclave used from a lab....
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23698116 - 10/01/16 07:11 PM (7 years, 3 months ago) |
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One more time on the important pressing issue, would you feel safe making tea from those fruits?
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23698117 - 10/01/16 07:11 PM (7 years, 3 months ago) |
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KISS. Always use KISS.
There is one way that has worked for me countless times. NO ADDITIVES NO PRESERVATIVES NO ARTIFICIAL COLORS.
Agar plate xfered to 10 jars of rye w/ gypsum. Jars of colonized CLEAN spawn mixed with substrate at a rate of 1:3 Substrate recipe: 45%hpoo, 45%verm, 10%gypsum brought to field capacity and pasteurized @ 150 for 90 minutes.
No need to case unless you live in really low humidity. Remember KISS.
Harvest many fruits and enjoy.
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23698120 - 10/01/16 07:12 PM (7 years, 3 months ago) |
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Quote:
ChicagoMan said: One more time on the important pressing issue, would you feel safe making tea from those fruits?
Personally no. Will they kill you? Probably not. If you are jonesing for some fungus pretty bad then this will teach you to have contingency measures.
Edited by RyeJar (10/01/16 07:13 PM)
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23698144 - 10/01/16 07:18 PM (7 years, 3 months ago) |
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Field capacity has been my Achilles heel, since my coir was in a giant block not a brick, I've been added way too much water each time knowing I would have to bring to field capacity with pillow case overnight (seemed very contamination prone) or squeeze each and every hand full as much as possible, basically until no drops came out of each scoop. Worked great for everything so far. The darn ghetto spore syringe was the culprit in this case.
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23698153 - 10/01/16 07:21 PM (7 years, 3 months ago) |
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Yes for sure contingency measures, like always having a plethora of more than I need always, but I've only been in it 6 months and have had many great results. Just can't stop taking and sharing!!!
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23698163 - 10/01/16 07:24 PM (7 years, 3 months ago) |
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This is how my substrate looks when hydrated to field capacity. You wont see the description on here but when i squeeze, i squeeze hard. I don't like the gentle squeeze test because gentle is so subjective. If you squeeze as hard as you can and a "stream" comes out then its too much. Note: I do err on the dry side.
Edited by RyeJar (10/01/16 07:25 PM)
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Inocuole
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23698310 - 10/01/16 08:22 PM (7 years, 3 months ago) |
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Quote:
RyeJar said: KISS. Always use KISS.
There is one way that has worked for me countless times. NO ADDITIVES NO PRESERVATIVES NO ARTIFICIAL COLORS.
Dafuq... I love artificial colors. Some boring unadventurous old people talk goin on in here..
 
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dankington
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Inocuole]
#23698320 - 10/01/16 08:26 PM (7 years, 3 months ago) |
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Represent

I use color to identify the media used.
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RyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: dankington]
#23698322 - 10/01/16 08:28 PM (7 years, 3 months ago) |
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It was just a saying. I dont advocate new users to try "boosting their yields" by adding this and that. Personally I use food coloring in my agar when working with multiple species.
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Mad Season
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
#23698570 - 10/01/16 10:14 PM (7 years, 3 months ago) |
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I have no food coloring cause what up?
Op all those fruits are edible. The tub on the other hand should be taken outside.
I guess you can scrub your room o.0 I totally wouldn't/haven't, and I've had much worse contaminations than that.
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Mad Season]
#23698592 - 10/01/16 10:24 PM (7 years, 3 months ago) |
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Thanks for the vote of confidence in those being alright. I know what looks good and bad on a shroom in general,but if I do break out a scope it would be easy, I havent made a slide in almost 5 years, miss it! I know that the whole point of microscopy/mycology anyway,but it's been years, lol.
Edited by ChicagoMan (10/01/16 10:27 PM)
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Mad Season
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23698599 - 10/01/16 10:28 PM (7 years, 3 months ago) |
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Psh even if there was mold on the shrooms, they're still edible. Trich is edible. Aspergillus is edible. Penicillium is edible. Almost every common green mold we see is edible. The thing to watch out for is airborne particles (mycotoxins) that you can inhale, and that's only if you have a compromised immune system. Ingesting them doesn't do shit.
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Mad Season]
#23698615 - 10/01/16 10:35 PM (7 years, 3 months ago) |
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Perfect thanks, being half blobish and since one has been fruitjng for 8 days the other for 7, it's damn hard to tell when to pick and how much, those little balls,knots stuck to substrate, they come too and are also gtg for eating, in a tea... This is all at my own collective reasoning. Thanks for any of your thoughts and ideas.
Edited by ChicagoMan (10/02/16 05:07 AM)
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23700127 - 10/02/16 12:53 PM (7 years, 3 months ago) |
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Do the white spots on the caps in first tub look like mold or mutation, if anyone can tell by the pic...
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Mad Season
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23700960 - 10/02/16 05:06 PM (7 years, 3 months ago) |
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Could be either or really. Could also be regular mycelium too. It's entirely up to you tho to see if you wanna try it. Regardless you really need to dry them out before ingesting.
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morty422
Wuba-luba-dub-dub!



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Re: Identify contam Plz, if trich maybe still make tea? [Re: Mad Season]
#23702307 - 10/03/16 02:21 AM (7 years, 3 months ago) |
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Quote:
Mad Season said:Regardless you really need to dry them out before ingesting.

Cracker Dry......
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: morty422]
#23703415 - 10/03/16 12:15 PM (7 years, 3 months ago) |
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As inhalation of the spores is the truly only real threat, is it dangerous to use a dehydrator, or should I cover dehydrator with something, or dehydrate in garage? Dehydrator in garage can't be bad idea. And yes, I assume all responsibility without question, thanks for just not Irving me blanket No answer just to be safe. I'm an educated adult, I can handle the truth and develop my own bad decisions if I I'm so inclined.
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ChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23704364 - 10/03/16 05:09 PM (7 years, 3 months ago) |
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So about to harvest flush, if u can call it that... White spots that feel like mushrooms and are not slimy should be relatively safe, if any green spots on caps throw those away, or just cut off bad part?
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Mad Season
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
#23704371 - 10/03/16 05:13 PM (7 years, 3 months ago) |
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Up to you lol. Personally when I had fruits with green mold on it, I just cut the contaminated parts off.
White myc I've never worried about
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ChicagoMan
Stranger



Registered: 03/22/16
Posts: 77
Last seen: 3 years, 3 months
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Mad Season]
#23706470 - 10/04/16 11:18 AM (7 years, 3 months ago) |
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Ugly tub 850g wet, better one 2075g wet...and they actually all look good, nothing had an odd smell...hoping for 8oz dry....of PE!!!
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