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OfflineRyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: dankington]
    #23697925 - 10/01/16 06:08 PM (7 years, 3 months ago)

Quote:

dankington said:
:shrug: my presto 16 or whatever is pretty cool…

Though using my buddy's 941 is pretty awesome :awesomenod:



I was rocking that presto inbetween the periods I lost my first autoclave and got my new one. It worked nice.  Didn't have too many complaints but I never did any technical tissue work with it either. 

I was always put off with the fact that cookers sucks in dirty air as they cool.  Causes you to take filtering precautions with everything you put into it.


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Invisibledankington
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
    #23697934 - 10/01/16 06:11 PM (7 years, 3 months ago)

yeah, but you work with what you can. Without it, I'd still be failing miserably with cakes. Now I just fail moderately :lol:

But seriously, the PC is fine too. Sterilizers are preferred, but we can work well with a PC.


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Invisiblefreespeech
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Re: Identify contam Plz, if trich maybe still make tea? [Re: dankington]
    #23698008 - 10/01/16 06:37 PM (7 years, 3 months ago)

If you want a better chance of success, go with a mainstream strain over PE. It's more difficult to grow PE.


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OfflineChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: freespeech]
    #23698061 - 10/01/16 06:52 PM (7 years, 3 months ago)

Everything has gone through my 16qt presto PC, couldn't justify 3x more $ on the AA, I'd love one, I'm a  Chem E, and autoclave medical instruments for cadaver use in my lab.  So agreement with wife is if and when presto fails, I get to buy any AA I want....kinda a prove ur going use it senerio.
They do the same thing, arguably just as well, 15psi is 15 psi...but the AA looks like a piece of lab equipment and last 3 lifetimes!!!


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OfflineRyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
    #23698077 - 10/01/16 06:57 PM (7 years, 3 months ago)

Quote:

ChicagoMan said:
Everything has gone through my 16qt presto PC, couldn't justify 3x more $ on the AA, I'd love one, I'm a  Chem E, and autoclave medical instruments for cadaver use in my lab.  So agreement with wife is if and when presto fails, I get to buy any AA I want....kinda a prove ur going use it senerio.
They do the same thing, arguably just as well, 15psi is 15 psi...but the AA looks like a piece of lab equipment and last 3 lifetimes!!!




I just googled shopped 15qt aa pressure cooker.  They start at 210.  They are selling a 25 qt aa autoclave on ebay brand new for 250.  Where is this 2x and 3x the price coming from?  Again, with patience I found my 25 qt for 150.

Edit: Just realized you meant 3x the price of the presto.  But the difference in price between an aa cooker and autoclave is negligible under the right conditions.


Edited by RyeJar (10/01/16 07:01 PM)


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OfflineChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
    #23698100 - 10/01/16 07:05 PM (7 years, 3 months ago)

I have had great success startingall this just back in March with 2 syringes, one Treasure Coast, the other PE.  Many less fruits with PE but total dry weights were almost the same.  Given the higher potential potency, I'm sticking with it.  I do still have multiple spore prints from the TC that I can utilize at some point.  Looking at getting grain prep done correctly and no contams  in any jars before spawning. Testing small then doing probably 5 66qt PE Mono's and maybe 5 of the TC.  Just need to get my Agar clean and isolated, and I have learned a lot about getting my moisture content perfect for substrate and for the grain jars.  Experience is obviously  the key! 
Best cleanest route?....spore/tissue to Agar, Agar to grain master,  grain jars spawned to substrate...many opinions I know, but let me know your favored methods, I've read litterly thousands of ways so again let me know if you have a personal favorite.
Also Agar to grain master, just use a wedge, or make Agar into Liquid innoculating?
Thanks for all replies!


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OfflineChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
    #23698110 - 10/01/16 07:09 PM (7 years, 3 months ago)

I was given a 6qt electric PC from mother-in-law, it was worth like $85 on a return for store credit, picked up the presto for $79. Once I have 10 perfect Mono's, I'll be able to justify any piece of equipment I would like, possibly a real autoclave used from a lab....


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OfflineChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
    #23698116 - 10/01/16 07:11 PM (7 years, 3 months ago)

One more time on the important pressing issue, would you feel safe making tea from those fruits?


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OfflineRyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
    #23698117 - 10/01/16 07:11 PM (7 years, 3 months ago)

KISS. Always use KISS.

There is one way that has worked for me countless times.  NO ADDITIVES NO PRESERVATIVES NO ARTIFICIAL COLORS.

Agar plate xfered to 10 jars of rye w/ gypsum.
Jars of colonized CLEAN spawn mixed with substrate at a rate of 1:3
    Substrate recipe: 45%hpoo, 45%verm, 10%gypsum brought to field capacity and pasteurized @ 150 for 90 minutes.

No need to case unless you live in really low humidity. Remember KISS.

Harvest many fruits and enjoy.


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OfflineRyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
    #23698120 - 10/01/16 07:12 PM (7 years, 3 months ago)

Quote:

ChicagoMan said:
One more time on the important pressing issue, would you feel safe making tea from those fruits?




Personally no.  Will they kill you?  Probably not.
If you are jonesing for some fungus pretty bad then this will teach you to have contingency measures. :wink:


Edited by RyeJar (10/01/16 07:13 PM)


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OfflineChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
    #23698144 - 10/01/16 07:18 PM (7 years, 3 months ago)

Field capacity has been my Achilles heel, since my coir was in a giant block not a brick, I've been added way too much water each time knowing I would have to bring to field capacity with pillow case overnight (seemed very contamination prone) or squeeze each and every hand full as much as possible, basically until no drops came out of each scoop.  Worked great for everything so far.  The darn ghetto spore syringe was the culprit in this case.


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OfflineChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
    #23698153 - 10/01/16 07:21 PM (7 years, 3 months ago)

Yes for sure contingency measures, like always having a plethora of more than I need always, but I've only been in it 6 months and have had many great results.  Just can't stop taking and sharing!!!


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OfflineRyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
    #23698163 - 10/01/16 07:24 PM (7 years, 3 months ago)

This is how my substrate looks when hydrated to field capacity.  You wont see the description on here but when i squeeze, i squeeze hard.  I don't like the gentle squeeze test because gentle is so subjective.  If you squeeze as hard as you can and a "stream" comes out then its too much.  Note: I do err on the dry side.



Edited by RyeJar (10/01/16 07:25 PM)


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InvisibleInocuole
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
    #23698310 - 10/01/16 08:22 PM (7 years, 3 months ago)

Quote:

RyeJar said:
KISS. Always use KISS.

There is one way that has worked for me countless times.  NO ADDITIVES NO PRESERVATIVES NO ARTIFICIAL COLORS.




Dafuq... I love artificial colors.  Some boring unadventurous old people talk goin on in here..



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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Invisibledankington
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Inocuole]
    #23698320 - 10/01/16 08:26 PM (7 years, 3 months ago)

Represent

       

I use color to identify the media used.


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OfflineRyeJar
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Re: Identify contam Plz, if trich maybe still make tea? [Re: dankington]
    #23698322 - 10/01/16 08:28 PM (7 years, 3 months ago)

It was just a saying.  I dont advocate new users to try "boosting their yields" by adding this and that.  Personally I use food coloring in my agar when working with multiple species.


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InvisibleMad Season
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Re: Identify contam Plz, if trich maybe still make tea? [Re: RyeJar]
    #23698570 - 10/01/16 10:14 PM (7 years, 3 months ago)

I have no food coloring cause what up?

Op all those fruits are edible. The tub on the other hand should be taken outside.

I guess you can scrub your room o.0 I totally wouldn't/haven't, and I've had much worse contaminations than that.


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OfflineChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Mad Season]
    #23698592 - 10/01/16 10:24 PM (7 years, 3 months ago)

Thanks for the vote of confidence in those being alright.  I know what looks good and bad on a shroom in general,but if I do break out a scope it would be easy, I havent made a slide in almost 5 years, miss it!  I know that the whole point of microscopy/mycology  anyway,but it's been years, lol.


Edited by ChicagoMan (10/01/16 10:27 PM)


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InvisibleMad Season
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Re: Identify contam Plz, if trich maybe still make tea? [Re: ChicagoMan]
    #23698599 - 10/01/16 10:28 PM (7 years, 3 months ago)

Psh even if there was mold on the shrooms, they're still edible. Trich is edible. Aspergillus is edible. Penicillium is edible. Almost every common green mold we see is edible. The thing to watch out for is airborne particles (mycotoxins) that you can inhale, and that's only if you have a compromised immune system. Ingesting them doesn't do shit.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineChicagoMan
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Re: Identify contam Plz, if trich maybe still make tea? [Re: Mad Season]
    #23698615 - 10/01/16 10:35 PM (7 years, 3 months ago)

Perfect thanks, being half blobish and since one has been fruitjng for 8 days the other for 7, it's damn hard to tell when to pick and how much, those little balls,knots stuck to substrate, they come too and are also gtg for eating, in a tea...
This is all at my own collective reasoning.  Thanks for any of your thoughts and ideas.


Edited by ChicagoMan (10/02/16 05:07 AM)


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