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OfflineAreos420
Stranger

Registered: 09/18/16
Posts: 16
Last seen: 7 years, 3 months
Water Culture (Honey Organic)
    #23697420 - 10/01/16 02:47 PM (7 years, 3 months ago)

Ok I have some serious questions This is my first time doing liquid cultures. so i Decided to to try out two different types of honey with two different strains very common strains B+ and GT. I used organic honey the most expensive i could find lol. then some cheap stater brothers natural honey which i figured would be fine. well after i pc'd them both i got sediments in the more expensive one which i figured the mycelium would eat anyways and the other no sediments. is picture with sediments after pc and mixed and settled again. i didnt take a pic of the other one just seemed pointless.
now this is after 5 days of growth on both   As you see it looks like the Mycelium looks to be eating the sediment fast i think at least if this is not whats happening please someone tell me and this is my other one with b+ injected into it as you see no growth at all and i used more spore solution maybe its the honey or maybe its that i waited 6hours after i injected them both? ever bit of information that would be said would be highly appreciated thanks


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OfflineAreos420
Stranger

Registered: 09/18/16
Posts: 16
Last seen: 7 years, 3 months
Re: Water Culture (Honey Organic) [Re: Areos420]
    #23697424 - 10/01/16 02:48 PM (7 years, 3 months ago)

And this is also after the next day which is today on the other one with sediments an it looks like its growing fast


Edited by Areos420 (10/01/16 02:49 PM)


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OfflineShineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Water Culture (Honey Organic) [Re: Areos420]
    #23697464 - 10/01/16 03:01 PM (7 years, 3 months ago)

If you were doing a side by side of which material would work better for a lc (expensive vs bargain) you should of use the same spore syringe to inoculate both.  Even that would be flawed because a vendor syringe is a ms solution with wildly different genetics in it.  Look up pastyplates and just work agar.  Lc's aren't reliable because until you put it on something you don't know if it harbors contaminants. At least if you had them on agar you could transfer good growth to a new plate.


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Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineAreos420
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Registered: 09/18/16
Posts: 16
Last seen: 7 years, 3 months
Re: Water Culture (Honey Organic) [Re: Shineonyoucrazy]
    #23697474 - 10/01/16 03:04 PM (7 years, 3 months ago)

see ive never grown mushrooms before this is my first time growing....i wouldn't even know were to start with agar trays i just finally got spores to california.


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OfflineShineonyoucrazy
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Registered: 09/22/16
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Re: Water Culture (Honey Organic) [Re: Areos420]
    #23697482 - 10/01/16 03:08 PM (7 years, 3 months ago)

Like i said look up pastyplates.  Ask the ingredients can be bought from the grocery store or elsewhere around town.  If you wanna do a pf style grow you'd be better off just inoculating with the ms and to hell with the lc made from ms. That's only giving the contaminants a head start if your going to shoot it into brf cakes anyways.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineAreos420
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Registered: 09/18/16
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Last seen: 7 years, 3 months
Re: Water Culture (Honey Organic) [Re: Shineonyoucrazy]
    #23697535 - 10/01/16 03:28 PM (7 years, 3 months ago)

well im starting off doing monotubs with substrates


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OfflineShineonyoucrazy
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Registered: 09/22/16
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Re: Water Culture (Honey Organic) [Re: Areos420]
    #23697554 - 10/01/16 03:34 PM (7 years, 3 months ago)

Then you'd definatly benifit from putting your syringe to agar to clean it up, then using the wedges to inoculate grains or to make a LI. Syringes are bound to have contaminants due to how they are produced.  Putting them on agar and cleaning them up is a way to avoid that along with proper sterile technique.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


Edited by Shineonyoucrazy (10/01/16 03:36 PM)


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OfflineAreos420
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Registered: 09/18/16
Posts: 16
Last seen: 7 years, 3 months
Re: Water Culture (Honey Organic) [Re: Shineonyoucrazy]
    #23700357 - 10/02/16 01:53 PM (7 years, 3 months ago)

guess i need to get study agar then and make a flow hood


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OfflineGreg
always learning
I'm a teapot


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Re: Water Culture (Honey Organic) [Re: Areos420]
    #23700360 - 10/02/16 01:55 PM (7 years, 3 months ago)

Make a still air box (SAB):
https://www.shroomery.org/forums/showflat.php/Number/20048771

Costs much less and unless you plan to do grain bags or massive volumes of jars, it will work just as well.


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Water Culture (Honey Organic) [Re: Areos420]
    #23700374 - 10/02/16 01:59 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:48 AM)


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OfflineShineonyoucrazy
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Registered: 09/22/16
Posts: 917
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Re: Water Culture (Honey Organic) [Re: Greg]
    #23700385 - 10/02/16 02:02 PM (7 years, 3 months ago)

:whathesaid:
Get a nice big ( i like my 110 qt)clear tub, cut your arm holes), sanitize it before use, and get to practicing! Best $10 i could of invested! Lol and you beat me to that just noticed after i posted!


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


Edited by Shineonyoucrazy (10/02/16 02:03 PM)


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OfflineAreos420
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Registered: 09/18/16
Posts: 16
Last seen: 7 years, 3 months
Re: Water Culture (Honey Organic) [Re: Shineonyoucrazy]
    #23701494 - 10/02/16 08:14 PM (7 years, 3 months ago)

thanks guys Alot ill post upcoming pics of my grows etc (: super excited for all this guys


Edited by Areos420 (10/02/16 08:16 PM)


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OfflineShineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Water Culture (Honey Organic) [Re: Areos420]
    #23701553 - 10/02/16 08:34 PM (7 years, 3 months ago)

:goodluck: you got this! Just keep reading and dont get discouraged


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineAreos420
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Registered: 09/18/16
Posts: 16
Last seen: 7 years, 3 months
Re: Water Culture (Honey Organic) [Re: Shineonyoucrazy]
    #23701852 - 10/02/16 10:17 PM (7 years, 3 months ago)

yes sir always will be into this hobby rather ti goes bad at first or not


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot


Registered: 07/06/16
Posts: 988
Re: Water Culture (Honey Organic) [Re: Areos420]
    #23701866 - 10/02/16 10:25 PM (7 years, 3 months ago)

Check out the links in my signature.

Use the Pastyplate's link and the Agar Portal.

These are game-changers for the beginner.


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OfflineMush Freak
Myco Psycho
I'm a teapot User Gallery


Registered: 12/29/11
Posts: 154
Last seen: 5 years, 9 days
Re: Water Culture (Honey Organic) [Re: morty422]
    #23702335 - 10/03/16 02:42 AM (7 years, 3 months ago)

Lookin good so far, bud. However the LC with no growth that is cloudy is definitely contaminated, sorry bout that.

However i'd definitely urge you to continue your plans and grow your LC out to a monotub, especially since you have gotten this far!

I do agree with the other guys that agar will help, as you can clean up any contaminates before you inoculate your spawn. But as of now, most of your LC looks good, and there is no reason not to try out a grow. I havent yet done any agar(though i'm planning on doing it with my next grow), and have had great success with doing spore syringe>LC>WBS>monotub/substrate. Of course success will vary from person to person because of many different variables, such as sterile technique, working environment(molds in home), etc.

Goodluck with your grow:D


--------------------

:mushroom2::mushroom2:Mono-tubs are LIFE:mushroom2::mushroom2:



Edited by Mush Freak (10/03/16 02:48 AM)


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OfflineAreos420
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Last seen: 7 years, 3 months
Re: Water Culture (Honey Organic) [Re: Mush Freak]
    #23703528 - 10/03/16 12:58 PM (7 years, 3 months ago)

yea man thanks imma post a picture of the LC now 3 days later the whole jar is practically full of Mycelium


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OfflineAreos420
Stranger

Registered: 09/18/16
Posts: 16
Last seen: 7 years, 3 months
Re: Water Culture (Honey Organic) [Re: Areos420]
    #23703604 - 10/03/16 01:22 PM (7 years, 3 months ago)

this is the  LC after the next 2 days showing alot of growth should i wait to use it till it stops growing


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OfflineMush Freak
Myco Psycho
I'm a teapot User Gallery


Registered: 12/29/11
Posts: 154
Last seen: 5 years, 9 days
Re: Water Culture (Honey Organic) [Re: Areos420]
    #23703674 - 10/03/16 01:37 PM (7 years, 3 months ago)

Quote:

Areos420 said:
this is the  LC after the next 2 days showing alot of growth should i wait to use it till it stops growing





Use it now!  :smile:


--------------------

:mushroom2::mushroom2:Mono-tubs are LIFE:mushroom2::mushroom2:



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InvisiblemushboyMDiscord
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Registered: 04/24/05
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OG Cultivator
Re: Water Culture (Honey Organic) [Re: Mush Freak]
    #23703832 - 10/03/16 02:13 PM (7 years, 3 months ago)

that looks like stale jizz. LC without agar cleanup is like ATM without douching first.its gonna be dirty.

but post the results!


Edited by mushboy (10/03/16 02:14 PM)


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