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Rxuser0439


Registered: 05/31/16
Posts: 7
Loc: Nebraska
Last seen: 7 years, 2 months
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Few different Teks with Many Questions
#23697197 - 10/01/16 01:08 PM (7 years, 3 months ago) |
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Ok sorry ahead of time for any dumb questions I've been searching forums and doing research for weeks now.So I bought 1 syringe from an unreliable vendor about 2 months ago and tried using Wbs tek. except without a pressure cooker.It did nothing no mold no contams but also no Mycliuem. they sa in an incubator for around 30 days with no signs so I threw them out and started again except with only half the syringe left about 5 ccs so I inoculated another 5 jars (1-1/4 pint) (1pint) (3halfpints) using the pf tek BRF. the 1/4 pint colonized 100 percent and I gave it another 7 days after that.The other half pints did nothing and the pint jars contaminated so I hit the forum again to see what may have happened.Basically Its been a trial and error process. I put my 1/4 pint cake dunked and broken up and mixed with paturized coir less then 1/4 brick and let sit in incubator for 6 days with tinfoil. I have some white patches in the coir but not much. I'm assuming not enough spawn to colonize the bulk substrate?
I Also bought all new jars again and got spores through a different vendor and decided to go with the Brf Tek with gypsum added to jars before sterilized for 120 mins in covered pot steam sterilzed. it has been 10 days now and all 5 jars I did look like this. around 55 to 65 percent colonized. No contamination I can see.This Grow seems on track. I'm thinking of doing 2 cakes and the other 3 jars as bulk with coir? any suggestions? Do they look right? 
I also bought spores from another vendor which cost me almost 50 bucks for 2 syringes I did the same process as the above except without the gypsum added.Also put sterilize machine bags over the jars and rubber banded them sealed. Now this patch has been sitting in the incubator for around 7 days now with only very slight mycelium growth in 2 jars. At first I thought maybe bad syringe. So I made a Liquid Culture and inoculated it with the same syringe. 2 ccs. to test the spores? It's only been a day so no growth yet. Then I thought maybe FAE which I didn't think about but during coloration it does need some FAE am I correct? so I removed the bags and rubber bands and replaced with tape to hopefully get some air. Now my LC I covered with several layers of tape and I'm thinking now that maybe I should just put one layer of for some air exchange but I'm not sure any recommendations. or help or anything would be greatly appreciated I'm a tattoo artist so the need to be sterile is always a constant factor.
Edited by Rxuser0439 (10/01/16 03:03 PM)
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: Few different Teks with Many Questions [Re: Rxuser0439]
#23697203 - 10/01/16 01:11 PM (7 years, 3 months ago) |
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Quoted directly from the rules. Pinned post at the top of the forum.
Quote:
3. Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself. Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning. Do not plug non sponsors.
You broke this rule 3 times in one post.
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Few different Teks with Many Questions [Re: Rxuser0439]
#23697204 - 10/01/16 01:12 PM (7 years, 3 months ago) |
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First suggestion edit those names out your post. Second edit out non sponsored vendors name
-------------------- Keep your feet on the ground and your head in the clouds
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Rxuser0439


Registered: 05/31/16
Posts: 7
Loc: Nebraska
Last seen: 7 years, 2 months
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first post sorry.
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Few different Teks with Many Questions [Re: Rxuser0439]
#23697223 - 10/01/16 01:22 PM (7 years, 3 months ago) |
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Fae isn't a factor til you birth them, but ge is. Take any bags covering off and set the jars out so they can have proper ge. Read up on the pf tek and the rules, don't wait your experiment to end before it ever really got to start. Good luck the micropore tape over the lc hole is fine, but if you do your pf jars correctly you don't need it on those. The dry verm layer acts as your filter.
-------------------- Keep your feet on the ground and your head in the clouds
Edited by Shineonyoucrazy (10/01/16 01:24 PM)
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: Few different Teks with Many Questions [Re: Rxuser0439]
#23697227 - 10/01/16 01:25 PM (7 years, 3 months ago) |
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Quote:
Registered: 05/31/16 Posts: 6
It isn't your first post and there isn't really an excuse for not reading the rules. Whatever though, you edited the post.
Quote:
Docs Wbs tek. except without a pressure cooker
You need to use a pressure cooker for grains, always. Follow the tek.
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incubator
Incubators are useless. Room temperature is perfect. Higher than 70-78 and you encourage bacteria.
Quote:
So I made a Liquid Culture and inoculated it with the same syringe
Liquid cultures from spores are doomed to fail every time. Spores are almost always slightly contaminated with mold or bacteria. This is because spores are collected from mushrooms grown in open air. Bacteria grows much faster than cubes in LC. Contaminants hide in LC very easily. You wont know until it fails.
Quote:
microspore tape
What the hell is microspore tape? It's micropore... I wouldn't have said anything but it obviously wasn't a typo because you wrote it that way twice.
Aside from that, micropore is fine to use as a filter as far as I know. Someone correct me if I'm wrong...
Basically, you can't just willy nilly all this stuff. Find a tek that is heavily vetted and recent (published within 3 or 4 years), and be successful by following it to a T. No modifications or substitutions.
If those cakes colonize fully, you can still fruit them in a SGFC: https://www.shroomery.org/forums/showflat.php/Number/20195542#20195542
Edited by Greg (10/01/16 01:38 PM)
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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Re: Few different Teks with Many Questions [Re: Greg]
#23697302 - 10/01/16 01:54 PM (7 years, 3 months ago) |
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My guess would be that you didn't shake up your syringe prior to using and the spores were all clumped together.
-------------------- Bug
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Rxuser0439


Registered: 05/31/16
Posts: 7
Loc: Nebraska
Last seen: 7 years, 2 months
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Re: Few different Teks with Many Questions [Re: Greg]
#23697439 - 10/01/16 02:54 PM (7 years, 3 months ago) |
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Thanks I appreciate it. and the link. I'm going to stick to just the Pf Tek like you said. Should the LC be thrown out?
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: Few different Teks with Many Questions [Re: Rxuser0439]
#23697628 - 10/01/16 04:11 PM (7 years, 3 months ago) |
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I would toss it if I were you.
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morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
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Re: Few different Teks with Many Questions [Re: Greg]
#23697698 - 10/01/16 04:37 PM (7 years, 3 months ago) |
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Quote:
Greg said: I would toss it if I were you.
LC from spores is fail city...
I would go back to the basics with correct jars and correct techniques following a tek that is successful.
Like Greg said... No substitutions - No deviations.
Do that and you will find yourself in a great spot. We've all seen failure at the beginning. This hobby is definitely not merciful if you don't follow the standardized techniques when you lack general/advanced knowledge.
Do it again-you'll see success if you follow the tek to a T.
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