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Shrooms4fun
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pre-poured Y petri dish
#23697115 - 10/01/16 12:36 PM (7 years, 3 months ago) |
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so im looking around for pre-poured Y petri dish's and cant seem to find any pre-poured has anyone had such luck to find a place that sells the Y petri dish pre-poured?
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Greg
always learning




Registered: 10/28/15
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If you can use petri dishes, you can pour them too. I know that's not the answer you wanted.
Nobody sells pre-poured sectioned dishes that I know of anyway. This would probably do better posted in the sponsors forum.
Pouring your own dishes will save you tons of money too. Shipping pre-poured dishes is expensive.
The one time I bought pre-poured dishes before I knew what I was doing, two out of 10 were contaminated by the time they got to me.
Edited by Greg (10/01/16 12:46 PM)
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enlightenment
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Re: pre-poured Y petri dish [Re: Greg]
#23697184 - 10/01/16 01:05 PM (7 years, 3 months ago) |
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 With a still air box you could have great success with petri dishes. I also don't know a vendor that sells pre poured dishes.
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mrmazdarx9
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  Why don't you just ask for someone to do all the work. If you can't pour Petri's there's no way you can use them
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: pre-poured Y petri dish [Re: mrmazdarx9]
#23697225 - 10/01/16 01:23 PM (7 years, 3 months ago) |
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Poring plates is easier and faster than no-pour once you get over the intimidation factor, which is really misplaced for the most part. Nothing hard about sterilizing a bottle of agar, cutting open a sleeve of sterile dishes, and the pouring the agar into the dishes.
I've never seen the point of divided dishes anyway. Even if one section gets mold, once you open the dish spores will become airborne, and transferring from a moldy dish is pretty easy anyway.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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enlightenment
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Re: pre-poured Y petri dish [Re: NumeroEno]
#23697228 - 10/01/16 01:26 PM (7 years, 3 months ago) |
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divided dishes are great for sectoring and other stuff... They save place and plates.. of course if there is mold chances are high that it will contaminate every sector if you open/move them.
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Kenetic
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Re: pre-poured Y petri dish [Re: NumeroEno]
#23697231 - 10/01/16 01:28 PM (7 years, 3 months ago) |
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pouring dishes is super easy. I use a sab and I've only had one petri contaminate out of at least 100 that I've done so far. And even that one only had one bacterial colony on it.
Do it once and you'll get over your fear.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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enlightenment
alchemist


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Re: pre-poured Y petri dish [Re: Kenetic]
#23697239 - 10/01/16 01:30 PM (7 years, 3 months ago) |
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Quote:
kenetic said: pouring dishes is super easy. I use a sab and I've only had one petri contaminate out of at least 100 that I've done so far. And even that one only had one bacterial colony on it.
Do it once and you'll get over your fear.
1/100 is a little optimistic, while i believe your statement.. If he is new to petri dishes 1/10 is realistic IMO.
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Kenetic
Nam Sayin



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Maybe so.
I just started agar about 2 months ago and this is my success rate.
1 out of 10 seems unacceptable to me.
Either way, pouring is way easier than it looks.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Quote:
enlightenment said: divided dishes are great for sectoring and other stuff... They save place and plates.. of course if there is mold chances are high that it will contaminate every sector if you open/move them.
IME they cost considerably more than undivided plates too. I dunno, I've just never been a big fan. I like the 100mm dishes the best, but I also like pouring 60mm dishes a little thicker too.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Kenetic
Nam Sayin



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Re: pre-poured Y petri dish [Re: NumeroEno]
#23697253 - 10/01/16 01:36 PM (7 years, 3 months ago) |
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I started out with 100 mm y dishes and now I use single 60mm. Love them both. The 60 mm tend to tip over easily if you aren't careful though
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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mrmazdarx9
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Registered: 05/15/16
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Quote:
enlightenment said:
Quote:
kenetic said: pouring dishes is super easy. I use a sab and I've only had one petri contaminate out of at least 100 that I've done so far. And even that one only had one bacterial colony on it.
Do it once and you'll get over your fear.
1/100 is a little optimistic, while i believe your statement.. If he is new to petri dishes 1/10 is realistic IMO.
I've yet to pour a bad plate so no as long as your meticulous with the sterile tek I don't see why that's optimistic. Over poured way over 100 and each one had sat in my cupboard till use. I let them all sit for at least a week before use, mainly because I know when I'm gonna have time to do transfers so I pour them a week in advance. Kenetic I completely agree and I think my technique is shit but 100% success pour rate so  And I'm new to agar even my first plates didn't contam till I tried my first inoculation.
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: pre-poured Y petri dish [Re: mrmazdarx9]
#23697259 - 10/01/16 01:39 PM (7 years, 3 months ago) |
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I recently poured 60 60mm plates and one of them got some aspergillus in it, probably because I turned off the flowhood before I wrapped them and a spore snuck up under the edge of the plate.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Greg
always learning




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Re: pre-poured Y petri dish [Re: NumeroEno]
#23697265 - 10/01/16 01:41 PM (7 years, 3 months ago) |
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I've poured over 200 and only one contaminated before I used it (single colony of bacteria). I've left plates in the SAB with no wrap for over a week before using them hah.
After transferring or inoculating is another story, but that's why we use agar, so contaminants show themselves and so we can isolate away from them.
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enlightenment
alchemist


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Re: pre-poured Y petri dish [Re: NumeroEno]
#23697268 - 10/01/16 01:41 PM (7 years, 3 months ago) |
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@NumeroEnoS They are more expensive. That's true. I also don't use them. I use 90mm dishes. Buying them in 25*20(500) packs. They are cheap and nice to use. I only had divided dishes once from a trade. It was a nice experience but i still like to use normal dishes like you. It's getting a little off topic now. The message to the OP is: Pouring dishes is not too complicated (I think we all agree).
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mrmazdarx9
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Re: pre-poured Y petri dish [Re: Greg]
#23697270 - 10/01/16 01:42 PM (7 years, 3 months ago) |
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Quote:
Greg said: I've poured over 200 and only one contaminated before I used it (single colony of bacteria). I've left plates in the SAB with no wrap for over a week before using them hah.
After transferring or inoculating is another story, but that's why we use agar, so contaminants show themselves and so we can isolate away from them.

Quote:
enlightenment said: @NumeroEnoS They are more expensive. That's true. I also don't use them. I use 90mm dishes. Buying them in 25*20(500) packs. They are cheap and nice to use. I only had divided dishes once from a trade. It was a nice experience but i still like to use normal dishes like you. It's getting a little off topic now. The message to the OP is: Pouring dishes is not too complicated (I think we all agree).
Definitely people have a weird fear of pouring plates, if no pour was the best way that's what labs would use
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: pre-poured Y petri dish [Re: mrmazdarx9]
#23697462 - 10/01/16 03:01 PM (7 years, 3 months ago) |
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Yeah, I'd almost call it a phobia. Back to the topic at hand, I wouldn't trust pre poured plates at all. It's so easy to do your own. It's even easy with a SAB, and mixing up your own nutrients is easy too.
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Mad Season
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Re: pre-poured Y petri dish [Re: NumeroEno]
#23697604 - 10/01/16 03:49 PM (7 years, 3 months ago) |
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I do no pours only, but when I started agar I did only pouring Petris. I too never had a blank plate contaminate. This was when I was a brand new noob at agar too lol. Only been from transferring with a dirty handled tool, or my hand goes over the open media by accident.
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Shrooms4fun
Stranger

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Re: pre-poured Y petri dish [Re: Mad Season]
#23704916 - 10/03/16 08:16 PM (7 years, 3 months ago) |
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i wouldnt say it is an actual fear of pouring the plates i simply want to dabble in them a little bit and see how things go possibly clone a fruit or something rather minor with minimal steps for me to screw up in the process to get the basics down a Y plate would give me 3 plates per plate to play with so i could order less plates that was the only thought behind that was all. One of the vendors offers single plates in sets of 4 for pre-pored for about $8 USD a set so maybe ill just get a couple of those to play around with.
as it stands im almost a week and a half into waiting for my BRF cakes to show any signs of colonization ( i dont think i screwed anything up here but who knows) i was just looking for something to do with spare time and another direction to go with this to become more self sufficient.
I see pre-pored MEAY and PDYA petri dishes on one of the vendor sites. What are the differences between them?
i have also found pre-made LC jars at another site that is a vendor that thought has also crossed my mind just to eliminate contamination variables from my end at the very least and simplify matters a little bit for myself.
a PC is soon on the list as well its only about $80 for a decent cheaper model https://www.amazon.com/dp/B0000BYCFU/ref=psdc_289825_t1_B00006ISG4 it seems to be a fair quality from what i have read
Edited by Shrooms4fun (10/03/16 11:06 PM)
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