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InvisibleBancaapi

Registered: 03/24/16
Posts: 66
Cloning in LC questions
    #23689410 - 09/29/16 06:38 AM (7 years, 3 months ago)

Thanks first for reading... And thanks for any input...

Okay...  There are APE in fruit and I am attempting to clone in LC... I think I have the process down sense this is my first attempt at cloning...  When I received the APE I really didn't do much research on them (I was just thinking they where PE) and years ago I use to just make spore syringes... I can see cultivation teks have changed over the years.. Okay to the question that I cannot seem to get a straight forward answer from all the research I have done over the last few months...

So when I clone the APE and create my own master culture when my master culture is done do I just do another clone and master culture or does this start to degrade?

Again thanks and sorry if it's a dumb question...


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"ummmm... I think we might have ate too many"


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
Trusted Cultivator
Re: Cloning in LC questions [Re: Bancaapi]
    #23689467 - 09/29/16 07:09 AM (7 years, 3 months ago)

Cloning in LC is not really a good idea. Not going to say it's not possible but it's full of potential vectors. You would be better served to clone to agar and then inoculate the LC with agar wedges.


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InvisibleBancaapi

Registered: 03/24/16
Posts: 66
Re: Cloning in LC questions [Re: Pastywhyte]
    #23689532 - 09/29/16 07:33 AM (7 years, 3 months ago)

Thanks for the reply... And I get what your saying. My issue is I am stuck without transportation or funds in the middle of nowhere (like 45 mins to the closest little tiny town nowhere) and without Funds I am unable to order online and I am getting close to deadlines of either a wait till I get funds and get new spores or b what I am attempting to do by using stuff and equipment I already have cause I am loving these APEs (strong little doods remind me of cyans I use to pick in Oregon when I was a teen) anyways I'm limited on what I can and can't do at the moment...

But the real question was not what I am or am not doing correctly is if I get a successful MC from a clone using LC when I run out of the original MC can I just do another clone? Or does this start to degrade over time? Somewhere I have a thought in my head at some point down the road if I keep cloning the same material it will start to degrade or am I completely wrong...  I am not an expert.. I just am able to follow instructions

Again thanks for any and all input!


--------------------
"ummmm... I think we might have ate too many"


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InvisibleJacobStorm
psychedelic cartel
I'm a teapot


Registered: 07/21/15
Posts: 1,499
Re: Cloning in LC questions [Re: Bancaapi]
    #23689559 - 09/29/16 07:42 AM (7 years, 3 months ago)

Your able to follow instructions? You got me beat. :popcorn:


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azur said: If you like 6th grade results, then 6th grade projects are great.


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InvisibleBancaapi

Registered: 03/24/16
Posts: 66
Re: Cloning in LC questions [Re: JacobStorm]
    #23689563 - 09/29/16 07:44 AM (7 years, 3 months ago)

Blah nevermind...


--------------------
"ummmm... I think we might have ate too many"


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
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Re: Cloning in LC questions [Re: Bancaapi]
    #23689564 - 09/29/16 07:44 AM (7 years, 3 months ago)

Yes cloning clones will lead to fast degredation. You are also not going to be able to expand forever, clone culture can be stored for a very long time and kept viable through careful expansion but, LC is about the worst way to store master culture.


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InvisibleBancaapi

Registered: 03/24/16
Posts: 66
Re: Cloning in LC questions [Re: Pastywhyte]
    #23689572 - 09/29/16 07:46 AM (7 years, 3 months ago)

Awesome... Thanks that's the part I have been missing...  Idk why I just had it in the back of my head that you cannot keep cloning clones... But I was unable for whatever reason be able to type in multiple ways in the search to get a direct answer is all.... Sorry for a stupid question...  And thanks for replying!


--------------------
"ummmm... I think we might have ate too many"


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InvisibleBancaapi

Registered: 03/24/16
Posts: 66
Re: Cloning in LC questions [Re: Bancaapi]
    #23689611 - 09/29/16 07:57 AM (7 years, 3 months ago)

Okay maybe saying master culture is my mistake here but whatever...  That's besides the point...  I am attempting to keep a non spore producing mushroom going further with easy to obtain materials...  Okay I get it I need more experience but I can only do what I can do...  Thanks all peace


--------------------
"ummmm... I think we might have ate too many"


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InvisibleJacobStorm
psychedelic cartel
I'm a teapot


Registered: 07/21/15
Posts: 1,499
Re: Cloning in LC questions [Re: Bancaapi]
    #23689623 - 09/29/16 07:59 AM (7 years, 3 months ago)

Just be patient dude, whats the hurry?


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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InvisibleBancaapi

Registered: 03/24/16
Posts: 66
Re: Cloning in LC questions [Re: JacobStorm]
    #23689642 - 09/29/16 08:04 AM (7 years, 3 months ago)

No hurry...  It was just a question


--------------------
"ummmm... I think we might have ate too many"


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