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OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
My culture fridge was a virtual graveyard
    #23689067 - 09/29/16 12:58 AM (7 years, 3 months ago)

Tossed (after trying) 25 plates- with more that don't look great. Still have some good plates but my library is practically wiped. Trying some crazy things with shrunken, dried cultures in a death gasp of a chance to preserve.

:justkillmenow:

Don't think my fridge was cold enough. Hopefully corrected. Need to start making slants. Ugg, very depressing. Also noticed those plates with thicker agar were still ok- will have to consider for masters.

Live. Learn. And then get Loads.

Still have yellow and red reishi, enoki, perhaps bsoy2 and 012a (crossing my fingers, but I took clones today from fruits of each so it will be preserved) Pheonix, my 2 slants (still need help with how to properly use them) and a couple LC's to trade to help get my culture library going again. Also just dropped a bunch of cultures- once they grow out I'll have some more to trade.


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Indoor Greenhouse Build 
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OfflineGreg
always learning
I'm a teapot

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Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
Re: My culture fridge was a virtual graveyard [Re: TravelAgency]
    #23689111 - 09/29/16 01:35 AM (7 years, 3 months ago)

I'll send you a wedge of everything I've got if you want.


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OfflineQuadman
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Registered: 04/23/16
Posts: 2,529
Loc: IL Flag
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Re: My culture fridge was a virtual graveyard [Re: Greg]
    #23689814 - 09/29/16 09:18 AM (7 years, 3 months ago)

That sucks. Don't give up on some of the dried ones. I have put them on agar then let them sit a few days.Then using sterilized water put a couple drops on the mycelium. They may come around, give them time.


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Edited by Quadman (09/29/16 09:19 AM)


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OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: My culture fridge was a virtual graveyard [Re: Quadman]
    #23690794 - 09/29/16 02:13 PM (7 years, 3 months ago)

Quote:

Greg said:
I'll send you a wedge of everything I've got if you want.





Dude that would be amazing

Quote:

Quadman said:
That sucks. Don't give up on some of the dried ones. I have put them on agar then let them sit a few days.Then using sterilized water put a couple drops on the mycelium. They may come around, give them time.




Yeah I made a few transfers- sterilized water sounds like a good idea might have to try that out.


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OfflineGreg
always learning
I'm a teapot

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Registered: 10/28/15
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Last seen: 3 months, 3 days
Re: My culture fridge was a virtual graveyard [Re: TravelAgency]
    #23691241 - 09/29/16 05:06 PM (7 years, 3 months ago)

Should be there in 3-5 days. :thumbup:



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Offlineanthiawe
friendly stranger
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Registered: 05/18/16
Posts: 652
Last seen: 2 years, 7 months
Re: My culture fridge was a virtual graveyard [Re: Greg]
    #23691275 - 09/29/16 05:12 PM (7 years, 3 months ago)

what can i do for a pack like that?


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TEK compendium


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OfflineGreg
always learning
I'm a teapot

Folding@home Statistics
Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
Re: My culture fridge was a virtual graveyard [Re: anthiawe]
    #23691281 - 09/29/16 05:14 PM (7 years, 3 months ago)

You're in Canada right? All I'd ask is that you pay shipping. Should be somewhere around 10 USD.


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OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: My culture fridge was a virtual graveyard [Re: Greg]
    #23691342 - 09/29/16 05:39 PM (7 years, 3 months ago)

Quote:

Greg said:
Should be there in 3-5 days. :thumbup:







That's amazing mate- thank you so much!


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OfflineMorePies
Stranger
Registered: 02/09/16
Posts: 808
Loc: TX Flag
Last seen: 1 month, 3 days
Re: My culture fridge was a virtual graveyard [Re: TravelAgency]
    #23691674 - 09/29/16 06:49 PM (7 years, 3 months ago)

Wow, super generous greg!


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Offlinekatbusa
TC Enthusiast
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Registered: 02/19/13
Posts: 172
Last seen: 6 years, 5 months
Re: My culture fridge was a virtual graveyard [Re: MorePies]
    #23691693 - 09/29/16 06:53 PM (7 years, 3 months ago)

What are you looking for as replacements? I have enoki, yellow oyst and an unknown strain of shiitaki i can spare.


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OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: My culture fridge was a virtual graveyard [Re: katbusa]
    #23691837 - 09/29/16 07:23 PM (7 years, 3 months ago)

Quote:

katbusa said:
What are you looking for as replacements? I have enoki, yellow oyst and an unknown strain of shiitaki i can spare.





I appreciate it but those actually survived for me! Thanks though!


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