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Offlinefernando
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Ok to fruit monotub too early?
    #23689050 - 09/29/16 12:49 AM (7 years, 4 months ago)

I've had several failed monotub grows. Mold starts to take over just as it's done colonizing the substrate. What would happen if I introduce fruiting conditions earlier? I.e. instead of waiting for 100% colonization I settle for 70% or something and then fruit. Will it just result in lower yields or perhaps not fruit at all?

I'd be ok with lower yields instead of having to throw it all out due to mold. The best thing to do would be to address the mold problem and rest assured I'll think long and hard about that, but in the mean time I'd like to grow at least something.

Oh, and for reference the bulk substrate is coir + verm + cube grain spawn. I've got great success with the grain spawn but the bulk substrate seems to get overrun by mold every time just before it's completely colonized. I probably have some mold spores in the grow room (just a normal living room) from earlier failed grows and getting rid of that seems impossible with all the fabric on furniture etc that can hold the spores.


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InvisibleBoogieman47
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Re: Ok to fruit monotub too early? [Re: fernando]
    #23689055 - 09/29/16 12:51 AM (7 years, 4 months ago)

Do you noc with syringe or use agar?


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Invisibleazur
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Re: Ok to fruit monotub too early? [Re: Boogieman47]
    #23689058 - 09/29/16 12:53 AM (7 years, 4 months ago)

"I've got great success with the grain spawn but the bulk substrate seems to get overrun by mold every time just before it's completely colonized."

That means you didn't have success with the spawn...


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InvisibleBoogieman47
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Re: Ok to fruit monotub too early? [Re: azur]
    #23689070 - 09/29/16 01:00 AM (7 years, 4 months ago)

Ya tubs wont go bad unless its bad spawn since youre not using hpoo it cant be improper pasteurization unless you have shitty coir and use the bucket but more then likely its your spawn


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Invisiblenobody83
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Re: Ok to fruit monotub too early? [Re: Boogieman47]
    #23689194 - 09/29/16 02:37 AM (7 years, 3 months ago)

Even with all that failure rate would be slim if spawn was clean


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InvisibleInocuole
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Re: Ok to fruit monotub too early? [Re: azur]
    #23689202 - 09/29/16 02:41 AM (7 years, 3 months ago)

Quote:

azur said:
"I've got great success with the grain spawn but the bulk substrate seems to get overrun by mold every time just before it's completely colonized."

That means you didn't have success with the spawn...




:whathesaid:


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OfflineMadSeasonStudent
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Re: Ok to fruit monotub too early? [Re: fernando]
    #23689698 - 09/29/16 08:24 AM (7 years, 3 months ago)

Are you using any lime in your monotubs? 

What recipe are you using when you make your sub?


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Offlinefernando
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Re: Ok to fruit monotub too early? [Re: MadSeasonStudent]
    #23689735 - 09/29/16 08:43 AM (7 years, 3 months ago)

Quote:

noob47 said:
Do you noc with syringe or use agar?




Agar.

Quote:

noob47 said:
Ya tubs wont go bad unless its bad spawn since youre not using hpoo it cant be improper pasteurization unless you have shitty coir and use the bucket but more then likely its your spawn




The spawn have looked healthy as far as I could tell. :undecided:

I once left some spawn 2 months in the jar, just to see what happened, and surely they eventually dried out but also got some nasty mold going. I have assumed that it came from outside through the filter though. The filter is just a vacuum dust bag that I've cut to fit and siliconed on. It has no problem maintaining contam-free for 1 month. That's what I have observed anyway. Of course I could have drawn the wrong conclusions.

Quote:

noob47 said:
Ya tubs wont go bad unless its bad spawn since youre not using hpoo it cant be improper pasteurization unless you have shitty coir and use the bucket but more then likely its your spawn




It's the coir blocks IKEA sells. Probably not the gold standard for mycological experiments but they come packaged in air tight plastic so after packaging they shouldn't be exposed much to outside contams. I also pasteurize in the oven. Put the oven on 75 degrees celcius and wait until the core temperature of the substrate reaches 60 - 71 degrees celcius (140-160F) and then keep it in that range for 1h. It usually takes several hours for the core temp to reach the lower pasteurization range.

Quote:

MadSeasonStudent said:
Are you using any lime in your monotubs? 

What recipe are you using when you make your sub?




No lime. I use some gypsum though.

Here's the recipe I follow:
1 coir brick from IKEA.
1L verm.
2.5L water.
a little gypsum.

Any input is valuable. I'm surprised to see many suspicions of contam'd grain spawn. Wouldn't the contams show fairly quickly? If it shows any trace of contams I don't use it. But if it (at least visibly) has been contam free for 3 weeks I have assumed that it was good to use.


Edited by fernando (09/29/16 08:45 AM)


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InvisibleBoogieman47
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Re: Ok to fruit monotub too early? [Re: fernando]
    #23689751 - 09/29/16 08:47 AM (7 years, 3 months ago)

Well if you are properly pasteurizing the sub then its the spawn unless youre sub is not cool enough when you mix it period there would be no other explaination take a pic of your agar and and spawn ...


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OfflineMadSeasonStudent
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Re: Ok to fruit monotub too early? [Re: Boogieman47]
    #23689762 - 09/29/16 08:52 AM (7 years, 3 months ago)

Hmmm, I did a mono about 2 weeks ago with what i thought was clean spawn and it turned green in the inside of the sub. A few days later my other mono turned green and im like wtf here. But i changed my recipe and used lime and not a problem since that.


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OfflineMadSeasonStudent
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Re: Ok to fruit monotub too early? [Re: MadSeasonStudent]
    #23689777 - 09/29/16 08:59 AM (7 years, 3 months ago)

You would know if your spawn is dirty. I mean, it doesnt take much to see it or smell it; and im sure you probably are familiar with those signs.  Instead of pasteurizing the sub, maybe just pressure cook it and see what happens?
That is where I would start. Lime and pc the sub and see if it makes a difference.

Coir is coir when you break it down but maybe there is something inside of it that is causing the issues.


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Invisibleazur
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Re: Ok to fruit monotub too early? [Re: MadSeasonStudent]
    #23689835 - 09/29/16 09:25 AM (7 years, 3 months ago)

Shit can hide very easily in spawn...


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A cube is NOT a cube.

FALL IN LOVE WITH LC
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Invisiblenobody83
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Re: Ok to fruit monotub too early? [Re: azur]
    #23690551 - 09/29/16 01:11 PM (7 years, 3 months ago)

To the point to where you barely have a clue if one at all


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Offlinefernando
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Re: Ok to fruit monotub too early? [Re: nobody83]
    #23690621 - 09/29/16 01:28 PM (7 years, 3 months ago)

Quote:

azur said:
Shit can hide very easily in spawn...




Quote:

nobody83 said:
To the point to where you barely have a clue if one at all




Perhaps you're both right that it's the grain spawn that is the contam source. But I'm using the same method to produce those that I have successfully used for shiitake and oysters. I'm not trying to argue, just thinking out "loud".

One way to narrow it down would be to ditch the coir in the next grow and add the grain spawn to straw pellets instead. That has worked well for oysters. If that turns out successful then it's likely the coir that's carrying the contams.

Thanks for your input guys, all of you.


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