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La Flama Blanca
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Registered: 01/15/16
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Stone producers on agar question
#23685929 - 09/28/16 09:27 AM (7 years, 4 months ago) |
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sorry if this has been asked and answered before but i've spent a couple hours over the last couple days looking for a post/thread with this answer and haven't found it. i really don't have the patience to keep looking.
anyways, my questions:
how do i differentiate between contams and desirable myc growth on agar for stone producers (tamps, jalisco, galindoi, ect), considering its normal, nay... favorable for stone producers to have non-white colored growth on agar? in my (extremely limited) experience; if its white, it's alright!
what colored growth should i transfer, and what colors do i not want to transfer?
I have heard that white myc growth is actually NOT a good candidate for growing stones on grains, as opposed to cube growth which we all know is ideally white and rhizomorphic. supposedly, neither of those traits are desirable for stone producers though? if anyone have experience trying to grow white rhizomorphic growing sclerotia producers on grains, please weigh in here.
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JacobStorm
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Registered: 07/21/15
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-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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tump
ban the undead



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Re: Stone producers on agar question [Re: JacobStorm]
#23686004 - 09/28/16 09:57 AM (7 years, 4 months ago) |
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Huh? Its a cube still you should still get white i believe
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azur
God of Fuck



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Re: Stone producers on agar question [Re: tump]
#23686024 - 09/28/16 10:03 AM (7 years, 4 months ago) |
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It's not a cube. OP, buy The Mushroom Cultivator. It has photographs of what you're looking for
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JacobStorm
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Re: Stone producers on agar question [Re: tump]
#23686030 - 09/28/16 10:05 AM (7 years, 4 months ago) |
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Quote:
tump said: Huh? Its a cube still you should still get white i believe
Here we go
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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leech911
Man with a plan



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Re: Stone producers on agar question [Re: JacobStorm]
#23686081 - 09/28/16 10:19 AM (7 years, 4 months ago) |
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Ooo I'd love to hear an answer. I'm very curious about growing sclerotia producers I'd love to see a successful sclerotia agar plate.
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tump
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Re: Stone producers on agar question [Re: leech911]
#23688259 - 09/28/16 08:05 PM (7 years, 4 months ago) |
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azur
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Re: Stone producers on agar question [Re: tump]
#23688881 - 09/28/16 11:02 PM (7 years, 3 months ago) |
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Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea
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JacobStorm
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Registered: 07/21/15
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Re: Stone producers on agar question [Re: azur]
#23689676 - 09/29/16 08:15 AM (7 years, 3 months ago) |
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Quote:
azur said: Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea

Thats purdy. Do you not use food coloring Azur?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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azur
God of Fuck



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Re: Stone producers on agar question [Re: JacobStorm]
#23689687 - 09/29/16 08:19 AM (7 years, 3 months ago) |
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Never have. Still don't understand why people do...
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JacobStorm
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Re: Stone producers on agar question [Re: azur]
#23689713 - 09/29/16 08:30 AM (7 years, 3 months ago) |
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I thought it was to help the mycelium stand out and thus identify what isnt mycelium?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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azur
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Re: Stone producers on agar question [Re: JacobStorm]
#23689722 - 09/29/16 08:33 AM (7 years, 3 months ago) |
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I have no idea. I thought it was just fun thing these kids did, like when you're 7 years old
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tump
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Re: Stone producers on agar question [Re: azur]
#23689733 - 09/29/16 08:41 AM (7 years, 3 months ago) |
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I use food color only when im doing a mixed agar plate runs like blue for pda and normal color for grain water
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spacechildo
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Re: Stone producers on agar question [Re: azur]
#23689776 - 09/29/16 08:59 AM (7 years, 3 months ago) |
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Quote:
azur said: I have no idea. I thought it was just fun thing these kids did, like when you're 7 years old

no pours are harder to see through the plastic and you cant put them up against light to see right through the agar/myc either so you can use dark food coloring as contrast.
I dont think I've seen anyone color the agar for proper petris.
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azur
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Re: Stone producers on agar question [Re: spacechildo]
#23689828 - 09/29/16 09:24 AM (7 years, 3 months ago) |
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Ah. I see. I tried no pour once. Too bulky and many of the containers tilted and got vacuumed.
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spacechildo
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Re: Stone producers on agar question [Re: azur]
#23689861 - 09/29/16 09:32 AM (7 years, 3 months ago) |
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tilted agar doesnt bother me much, I use spores and not syringes so they stay where I want them to on the plate. vacuums shouldn't happen if you have a filtered hole in your plates.
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azur
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Re: Stone producers on agar question [Re: spacechildo]
#23689906 - 09/29/16 09:53 AM (7 years, 3 months ago) |
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Oh. I don't remember, but I don't think I did. Oh well. I have a big ass hood, so there's no reason for me to ever try it again
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spacechildo
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Re: Stone producers on agar question [Re: azur]
#23689934 - 09/29/16 10:03 AM (7 years, 3 months ago) |
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shipping's what makes me choose no pour over petris. even a 500 carton the shipping costs more than the petris does. *i wanna be americano, americano...but was born in Italy...*
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Buger
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Registered: 09/04/16
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Re: Stone producers on agar question [Re: spacechildo]
#23689956 - 09/29/16 10:09 AM (7 years, 3 months ago) |
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Youll know, if you have used synthetic filters or tyvek, give it a wiff, should smell mushroomy or grainy, sweet and sour, toss out. As for coloration, its pretty obvious.
B
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
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Re: Stone producers on agar question [Re: spacechildo]
#23689967 - 09/29/16 10:13 AM (7 years, 3 months ago) |
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I did no-pour for a while but it irritated the fuck out of me because I could only do like 10 plates, and the mini rounds weren't locally available so I had to order them, and then they're like impossible to see through. Pouring 50 petris is less of a hassle than doing 8 no pour plates. I was using food coloring for a while to help spot bacteria but now my eyes are trained better and I don't need it.
As for the OP, once you get a good grasp of the macroscopic differences between mold and mushroom mycelium, differentiating stone producer myc and mushroom myc from mold is easy. Mold will always sporulate on the plate.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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JacobStorm
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Re: Stone producers on agar question [Re: spacechildo]
#23690001 - 09/29/16 10:22 AM (7 years, 3 months ago) |
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I just made pasty plates yesterday but couldnt find mini rounds here either. So I used some 1/4th pint jars I had. it was fairly easy, God damn holy fuck a lot of foil tho.
Quote:
spacechildo said: I use spores and not syringes so they stay where I want them to on the plate.
We can just drop spores from a print on agar? I have no reason too, but I thought the syringe had to come first.
Edit: Nevermind thats retarded because we use swabs on agar
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
Edited by JacobStorm (09/29/16 10:23 AM)
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La Flama Blanca
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Re: Stone producers on agar question [Re: Buger]
#23690375 - 09/29/16 12:19 PM (7 years, 3 months ago) |
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Quote:
Buger said: Youll know, if you have used synthetic filters or tyvek, give it a wiff, should smell mushroomy or grainy, sweet and sour, toss out. As for coloration, its pretty obvious.
B
so if the petri dish smells sweet and sour, toss it out. if it smells like mushrooms, there is good sclerotia producer growth to transfer on the plate.... thanks for the hot tip...
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La Flama Blanca
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Re: Stone producers on agar question [Re: azur]
#23690382 - 09/29/16 12:22 PM (7 years, 3 months ago) |
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Quote:
azur said: Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea

hmmm doesn't look like there are any stones on the plate? or any color other than off-white? doesnt match the description of a good transfer candidate based on the reading i've been doing on the subject... did that plate end up producing good stones and/or fruits?
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azur
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It's grown out all the way. Yes, fantastic culture.
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nobody83
stranger danger


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Re: Stone producers on agar question [Re: azur]
#23690453 - 09/29/16 12:39 PM (7 years, 3 months ago) |
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Looks like one
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
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Quote:
La Flama Blanca said:
Quote:
azur said: Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea

hmmm doesn't look like there are any stones on the plate? or any color other than off-white? doesnt match the description of a good transfer candidate based on the reading i've been doing on the subject... did that plate end up producing good stones and/or fruits?
Not every kickass culture will produce invitro stones or fruits right away, but if you let the plate fully colonize and sit around for a month or two you'll get one or the other and maybe both.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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azur
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Re: Stone producers on agar question [Re: NumeroEno]
#23690469 - 09/29/16 12:44 PM (7 years, 3 months ago) |
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I have some plates with nice long pins on them. But I plan on cloning and didn't want to waste a plate by opening it for op
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Curious Shroomer
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Re: Stone producers on agar question [Re: azur]
#23690590 - 09/29/16 01:21 PM (7 years, 3 months ago) |
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My myc is white and fluffy and smells a little bit like mushrooms. Got the Myc from a Jar thats producing Stones. Currently having 3 Plates i transfered from sitting around. Waiting if it produces Stones or Fruits. Hoping for Fruits so i can get some Prints.
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blackout


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Re: Stone producers on agar question [Re: spacechildo]
#23690803 - 09/29/16 02:16 PM (7 years, 3 months ago) |
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Quote:
spacechildo said: I dont think I've seen anyone color the agar for proper petris.
Here are some, which just happen to be stone producers, cloned from commercial ssclerotia most probably ATL#7


It does help with the semi transparent dishes, which most of that batch of agar was used for.
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NumeroEno
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Re: Stone producers on agar question [Re: azur]
#23690813 - 09/29/16 02:18 PM (7 years, 3 months ago) |
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Quote:
azur said: I have some plates with nice long pins on them. But I plan on cloning and didn't want to waste a plate by opening it for op
That's exactly what my current culture is from. The pins went like noodles around the whole plate. Cloned a chunk of stem and it must have been contaminated because the new plate got moldy as fuck, but I did manage to salvage it and now I have some of that culture on grain.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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La Flama Blanca
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Re: Stone producers on agar question [Re: NumeroEno]
#23694325 - 09/30/16 03:21 PM (7 years, 3 months ago) |
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Quote:
NumeroEno said:
Quote:
La Flama Blanca said:
Quote:
azur said: Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea

hmmm doesn't look like there are any stones on the plate? or any color other than off-white? doesnt match the description of a good transfer candidate based on the reading i've been doing on the subject... did that plate end up producing good stones and/or fruits?
Not every kickass culture will produce invitro stones or fruits right away, but if you let the plate fully colonize and sit around for a month or two you'll get one or the other and maybe both.
thanks, i will try that! i've been trying to follow RR's tek/method (forgot where he posted it but i read him say it a few times). he says that after 2-3 transfers, you should already see stones formed long before the transferred culture is even close to fully colonizing its new plate, and then use one of those stone to inoculate the next thing (grains, lc, more agar plates, ect). haven't had any success trying it that way so i'll try yours...i do worry about using a month or two old plate tho??? seems like that would be a far from ideal plate to noc something up with due to senescence and what not, no?
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RyeJar
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I use food coloring whenever there are more than 3 or 4 species I am working with at the moment for quick identification. Makes it a lot easier than sharpie marks all over the dish.
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