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InvisibleJacobStorm
psychedelic cartel
I'm a teapot


Registered: 07/21/15
Posts: 1,499
Re: Stone producers on agar question [Re: spacechildo]
    #23690001 - 09/29/16 10:22 AM (7 years, 3 months ago)

I just made pasty plates yesterday but couldnt find mini rounds here either. So I used some 1/4th pint jars I had. it was fairly easy, God damn holy fuck a lot of foil tho.

Quote:

spacechildo said: I use spores and not syringes so they stay where I want them to on the plate.





We can just drop spores from a print on agar? I have no reason too, but I thought the syringe had to come first.

Edit: Nevermind thats retarded because we use swabs on agar :facepalm:


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azur said: If you like 6th grade results, then 6th grade projects are great.


Edited by JacobStorm (09/29/16 10:23 AM)


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OfflineLa Flama Blanca
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Registered: 01/15/16
Posts: 487
Last seen: 10 months, 28 days
Re: Stone producers on agar question [Re: Buger]
    #23690375 - 09/29/16 12:19 PM (7 years, 3 months ago)

Quote:

Buger said:
Youll know, if you have used synthetic filters or tyvek, give it a wiff, should smell mushroomy or grainy, sweet and sour, toss out. As for coloration, its pretty obvious.


B




so if the petri dish smells sweet and sour, toss it out. if it smells like mushrooms, there is good sclerotia producer growth to transfer on the plate....
thanks for the hot tip...
:holyshit:


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OfflineLa Flama Blanca
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Re: Stone producers on agar question [Re: azur]
    #23690382 - 09/29/16 12:22 PM (7 years, 3 months ago)

Quote:

azur said:
Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea






hmmm doesn't look like there are any stones on the plate? or any color other than off-white? doesnt match the description of a good transfer candidate based on the reading i've been doing on the subject... :confused: did that plate end up producing good stones and/or fruits?


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Invisibleazur
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Registered: 04/21/12
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Re: Stone producers on agar question [Re: La Flama Blanca]
    #23690389 - 09/29/16 12:24 PM (7 years, 3 months ago)

It's grown out all the way.
Yes, fantastic culture.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Invisiblenobody83
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Registered: 03/15/14
Posts: 1,486
Loc: around town Flag
Re: Stone producers on agar question [Re: azur]
    #23690453 - 09/29/16 12:39 PM (7 years, 3 months ago)

Looks like one


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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: Stone producers on agar question [Re: La Flama Blanca]
    #23690466 - 09/29/16 12:43 PM (7 years, 3 months ago)

Quote:

La Flama Blanca said:
Quote:

azur said:
Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea






hmmm doesn't look like there are any stones on the plate? or any color other than off-white? doesnt match the description of a good transfer candidate based on the reading i've been doing on the subject... :confused: did that plate end up producing good stones and/or fruits?




Not every kickass culture will produce invitro stones or fruits right away, but if you let the plate fully colonize and sit around for a month or two you'll get one or the other and maybe both.


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

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Invisibleazur
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Re: Stone producers on agar question [Re: NumeroEno]
    #23690469 - 09/29/16 12:44 PM (7 years, 3 months ago)

I have some plates with nice long pins on them. But I plan on cloning and didn't want to waste a plate by opening it for op


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineCurious Shroomer
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Registered: 05/19/16
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Re: Stone producers on agar question [Re: azur]
    #23690590 - 09/29/16 01:21 PM (7 years, 3 months ago)

My myc is white and fluffy and smells a little bit like mushrooms. Got the Myc from a Jar thats producing Stones. Currently having 3 Plates i transfered from sitting around. Waiting if it produces Stones or Fruits. Hoping for Fruits so i can get some Prints.


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Offlineblackout
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Re: Stone producers on agar question [Re: spacechildo]
    #23690803 - 09/29/16 02:16 PM (7 years, 3 months ago)

Quote:

spacechildo said:
I dont think I've seen anyone color the agar for proper petris.



Here are some, which just happen to be stone producers, cloned from commercial ssclerotia most probably ATL#7




It does help with the semi transparent dishes, which most of that batch of agar was used for.


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InvisibleNumeroEno
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Registered: 07/24/14
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Re: Stone producers on agar question [Re: azur]
    #23690813 - 09/29/16 02:18 PM (7 years, 3 months ago)

Quote:

azur said:
I have some plates with nice long pins on them. But I plan on cloning and didn't want to waste a plate by opening it for op




That's exactly what my current culture is from. The pins went like noodles around the whole plate. Cloned a chunk of stem and it must have been contaminated because the new plate got moldy as fuck, but I did manage to salvage it and now I have some of that culture on grain.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineLa Flama Blanca
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Registered: 01/15/16
Posts: 487
Last seen: 10 months, 28 days
Re: Stone producers on agar question [Re: NumeroEno]
    #23694325 - 09/30/16 03:21 PM (7 years, 3 months ago)

Quote:

NumeroEno said:
Quote:

La Flama Blanca said:
Quote:

azur said:
Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea






hmmm doesn't look like there are any stones on the plate? or any color other than off-white? doesnt match the description of a good transfer candidate based on the reading i've been doing on the subject... :confused: did that plate end up producing good stones and/or fruits?




Not every kickass culture will produce invitro stones or fruits right away, but if you let the plate fully colonize and sit around for a month or two you'll get one or the other and maybe both.




thanks, i will try that! i've been trying to follow RR's tek/method (forgot where he posted it but i read him say it a few times). he says that after 2-3 transfers, you should already see stones formed long before the transferred culture is even close to fully colonizing its new plate, and then use one of those stone to inoculate the next thing (grains, lc, more agar plates, ect). haven't had any success trying it that way so i'll try yours...i do worry about using a month or two old plate tho??? seems like that would be a far from ideal plate to noc something up with due to senescence and what not, no?


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OfflineRyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 10 months, 2 hours
Re: Stone producers on agar question [Re: La Flama Blanca]
    #23694449 - 09/30/16 03:57 PM (7 years, 3 months ago)

I use food coloring whenever there are more than 3 or 4 species I am working with at the moment for quick identification.  Makes it a lot easier than sharpie marks all over the dish.


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