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OfflineLa Flama Blanca
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Stone producers on agar question
    #23685929 - 09/28/16 09:27 AM (7 years, 4 months ago)

sorry if this has been asked and answered before but i've spent a couple hours over the last couple days looking for a post/thread with this answer and haven't found it. i really don't have the patience to keep looking.

anyways, my questions:

how do i differentiate between contams and desirable myc growth on agar for stone producers (tamps, jalisco, galindoi, ect), considering its normal, nay... favorable for stone producers to have non-white colored growth on agar? in my (extremely limited) experience; if its white, it's alright!

what colored growth should i transfer, and what colors do i not want to transfer?

I have heard that white myc growth is actually NOT a good candidate for growing stones on grains, as opposed to cube growth which we all know is ideally white and rhizomorphic. supposedly, neither of those traits are desirable for stone producers though? if anyone have experience trying to grow white rhizomorphic growing sclerotia producers on grains, please weigh in here.


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InvisibleJacobStorm
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Re: Stone producers on agar question [Re: La Flama Blanca]
    #23685970 - 09/28/16 09:44 AM (7 years, 4 months ago)

:popcorn:


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Offlinetump
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Re: Stone producers on agar question [Re: JacobStorm]
    #23686004 - 09/28/16 09:57 AM (7 years, 4 months ago)

Huh? Its a cube still you should still get white i believe


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Invisibleazur
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Re: Stone producers on agar question [Re: tump]
    #23686024 - 09/28/16 10:03 AM (7 years, 4 months ago)

It's not a cube.
OP, buy The Mushroom Cultivator. It has photographs of what you're looking for


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InvisibleJacobStorm
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Re: Stone producers on agar question [Re: tump]
    #23686030 - 09/28/16 10:05 AM (7 years, 4 months ago)

Quote:

tump said:
Huh? Its a cube still you should still get white i believe




Here we go :facepalm:


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azur said: If you like 6th grade results, then 6th grade projects are great.


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Offlineleech911
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Re: Stone producers on agar question [Re: JacobStorm]
    #23686081 - 09/28/16 10:19 AM (7 years, 4 months ago)

Ooo I'd love to hear an answer. I'm very curious about growing sclerotia producers I'd love to see a successful sclerotia agar plate.
:threadmonitor:


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Offlinetump
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Re: Stone producers on agar question [Re: leech911]
    #23688259 - 09/28/16 08:05 PM (7 years, 4 months ago)



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Invisibleazur
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Re: Stone producers on agar question [Re: tump]
    #23688881 - 09/28/16 11:02 PM (7 years, 3 months ago)

Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea


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A cube is NOT a cube.

FALL IN LOVE WITH LC
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InvisibleJacobStorm
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Re: Stone producers on agar question [Re: azur]
    #23689676 - 09/29/16 08:15 AM (7 years, 3 months ago)

Quote:

azur said:
Here's a tamp plate. It's a little older and has knots on it. Potato agar. Will appear darker on mea





Thats purdy. Do you not use food coloring Azur?


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Ethnobotanical garden forum

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azur said: If you like 6th grade results, then 6th grade projects are great.


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Invisibleazur
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Re: Stone producers on agar question [Re: JacobStorm]
    #23689687 - 09/29/16 08:19 AM (7 years, 3 months ago)

Never have. Still don't understand why people do...


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FALL IN LOVE WITH LC
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InvisibleJacobStorm
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Re: Stone producers on agar question [Re: azur]
    #23689713 - 09/29/16 08:30 AM (7 years, 3 months ago)

I thought it was to help the mycelium stand out and thus identify what isnt mycelium?


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azur said: If you like 6th grade results, then 6th grade projects are great.


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Invisibleazur
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Re: Stone producers on agar question [Re: JacobStorm]
    #23689722 - 09/29/16 08:33 AM (7 years, 3 months ago)

I have no idea. I thought it was just fun thing these kids did, like when you're 7 years old
:shrug:


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A cube is NOT a cube.

FALL IN LOVE WITH LC
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Offlinetump
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Re: Stone producers on agar question [Re: azur]
    #23689733 - 09/29/16 08:41 AM (7 years, 3 months ago)

I use food color only when im doing a mixed agar plate runs like blue for pda and normal color for grain water


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Offlinespacechildo
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Re: Stone producers on agar question [Re: azur]
    #23689776 - 09/29/16 08:59 AM (7 years, 3 months ago)

Quote:

azur said:
I have no idea. I thought it was just fun thing these kids did, like when you're 7 years old
:shrug:




no pours are harder to see through the plastic and you cant put them up against light to see right through the agar/myc either so you can use dark food coloring as contrast.

I dont think I've seen anyone color the agar for proper petris.


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Invisibleazur
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Re: Stone producers on agar question [Re: spacechildo]
    #23689828 - 09/29/16 09:24 AM (7 years, 3 months ago)

Ah. I see.
I tried no pour once. Too bulky and many of the containers tilted and got vacuumed.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
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Offlinespacechildo
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Re: Stone producers on agar question [Re: azur]
    #23689861 - 09/29/16 09:32 AM (7 years, 3 months ago)

tilted agar doesnt bother me much, I use spores and not syringes so they stay where I want them to on the plate.
vacuums shouldn't happen if you have a filtered hole in your plates.


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Invisibleazur
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Re: Stone producers on agar question [Re: spacechildo]
    #23689906 - 09/29/16 09:53 AM (7 years, 3 months ago)

Oh. I don't remember, but I don't think I did. Oh well. I have a big ass hood, so there's no reason for me to ever try it again


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Offlinespacechildo
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Re: Stone producers on agar question [Re: azur]
    #23689934 - 09/29/16 10:03 AM (7 years, 3 months ago)

shipping's what makes me choose no pour over petris. even a 500 carton the shipping costs more than the petris does.
*i wanna be americano, americano...but was born in Italy...*  :celery:


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OfflineBuger
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Re: Stone producers on agar question [Re: spacechildo]
    #23689956 - 09/29/16 10:09 AM (7 years, 3 months ago)

Youll know, if you have used synthetic filters or tyvek, give it a wiff, should smell mushroomy or grainy, sweet and sour, toss out. As for coloration, its pretty obvious.


B


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InvisibleNumeroEno
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Re: Stone producers on agar question [Re: spacechildo]
    #23689967 - 09/29/16 10:13 AM (7 years, 3 months ago)

I did no-pour for a while but it irritated the fuck out of me because I could only do like 10 plates, and the mini rounds weren't locally available so I had to order them, and then they're like impossible to see through. Pouring 50 petris is less of a hassle than doing 8 no pour plates. I was using food coloring for a while to help spot bacteria but now my eyes are trained better and I don't need it.

As for the OP, once you get a good grasp of the macroscopic differences between mold and mushroom mycelium, differentiating stone producer myc and mushroom myc from mold is easy. Mold will always sporulate on the plate.


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