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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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Offlinekaius711
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Registered: 09/15/16
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Agar substitute method question
    #23682128 - 09/27/16 03:54 AM (7 years, 5 months ago)

Greetings. i have found an agar substitute method: https://www.shroomery.org/8514/Agar-substitute

the problem is however, this method requires tyvek, something that seems crucial to avoid contaminants and something i cannot get a hold of. And no i cannot just go to my post office and get it for free i live in Australia. is anybody able to think of a different way of going about this method without tyvek or tell me if its really necessary for the method to work as i am well and truly stuck.
thank you for your assistance.

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Invisiblefilthyknees
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Re: Agar substitute method question [Re: kaius711]
    #23682284 - 09/27/16 06:38 AM (7 years, 5 months ago)

You can use a number of filter material. If you cannot find tyvek gas exchange on Petri dishes can be achieved using poly fill, sfd or micropore tape.  :thumbup:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
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Offlinekaius711
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Re: Agar substitute method question [Re: filthyknees]
    #23682290 - 09/27/16 06:41 AM (7 years, 5 months ago)

do you suggest i cover the inoculation holes with micropore tape because i struggle to think how you can use the micropore tape in the same way as tyvek as depicted in the tek. and also do you think instead of ground up brown rice, i cna just use brown rice flour?

Edited by kaius711 (09/27/16 06:43 AM)

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Invisiblefilthyknees
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Re: Agar substitute method question [Re: kaius711]
    #23682293 - 09/27/16 06:43 AM (7 years, 5 months ago)

I know any of those filters will work. I use Petri dishes and parafilm, so that's what I would do.

I don't know about any substitutuions. I use mainly premix agar from sponsors.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

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InvisibleFantastic Mr. Fox
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Posts: 3,225
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Re: Agar substitute method question [Re: filthyknees]
    #23682882 - 09/27/16 11:06 AM (7 years, 5 months ago)

Micropore tape works . It's all I've used for the past 5 years


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:

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InvisiblebodhisattaMDiscordReddit
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Re: Agar substitute method question [Re: Fantastic Mr. Fox]
    #23682934 - 09/27/16 11:23 AM (7 years, 5 months ago)

Last link in my signature. Avoid the 8-10 year old info on the main site archives. Search the forums

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Offlinethemadextractor
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Re: Agar substitute method question [Re: filthyknees]
    #23683208 - 09/27/16 01:25 PM (7 years, 5 months ago)

All I use is micro pore tape. Works very well.  You can Double up if you like but I've even reused the tape several times without replacing. Haven't had contam issues so far.

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InvisibleFantastic Mr. Fox
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Re: Agar substitute method question [Re: themadextractor]
    #23683249 - 09/27/16 01:40 PM (7 years, 5 months ago)

Always double.  One on the outside of the lid, and one inside.


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:

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Offlinekaius711
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I'm a teapot


Registered: 09/15/16
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Re: Agar substitute method question [Re: Fantastic Mr. Fox]
    #23684583 - 09/27/16 09:05 PM (7 years, 5 months ago)

thank you for your assistance

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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


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