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Offlinemusiclover420
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Re: An average footlong at subway is unhealthy as fuck [Re: Prisoner#1]
    #23686957 - 09/28/16 02:28 PM (7 years, 4 months ago)

Quote:

Prisoner#1 said:
hope your head doesnt explode when you learn that
aspartame is non GMO and gluten free ya hipster





Yes and it is also made from genetically modified E Coli :lol:

Aspartame patent

Quote:

The artificial sweetener aspartame, a dipeptide with the formula Asp-Phe-me, is produced using a cloned microorganism.




Quote:

A microorganism according to claim 14 wherein said microorganism is a strain of E.coli K12.




--------------------
Don't worry about me, I've got all that I need. And I'm singing my song to the sky

You know how it feels, With the breeze of the sun in your eyes. Not minding that time's passing by

I've got all and more, My smile, just as before. Is all that I carry with me

I talk to myself, I need nobody else. I'm lost and I'm mine, yes I'm free



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Invisibleshadyy
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Re: An average footlong at subway is unhealthy as fuck [Re: musiclover420] * 1
    #23686972 - 09/28/16 02:31 PM (7 years, 4 months ago)

I usually hit up this food truck owned by legitimate Mexicans.

I think it's like 7 bucks a burrito, but these things are monstrous.


--------------------

ga ga ga eets eets how you gone be mad on vacation?
MONICA COULDN'T TELL TIME UNTIL SHE WAS 13


Edited by shadyy (09/28/16 02:59 PM)


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OfflineStarstepper
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Re: An average footlong at subway is unhealthy as fuck [Re: Crystal G]
    #23687000 - 09/28/16 02:36 PM (7 years, 4 months ago)

Quote:

Crystal G said:
When I was living in the Mexican neighborhood  I lived right next door to this taco shop. They were literally my next door neighbors that renovated their home to turn it into a walk in business.

I always ordered the tripe, tongue, and beef cheek tacos from them. 75 cents a taco, and unbelievably on taco Tuesdays the price gets even cheaper,it drops down to like 62 cents or something.

Then there's another places where tacos are a dollar but they slice meat fresh off the adobada rotating meat revolver like this






When you see the meat revolver it is game on!


--------------------
:darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside::darkside:


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OnlineLogicaL ChaosM
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Re: An average footlong at subway is unhealthy as fuck [Re: Repertoire89]
    #23687293 - 09/28/16 03:54 PM (7 years, 4 months ago)

Quote:

Repertoire89 said:
In San Diego I lived next to a decent Mexican restaurant, it was fucking great for hangovers.
They were literally 30ft from my bed. Good, cheap food.

Lived on a dock, could wake up, grab some good food and be hanging out on the beach with a spliff in 10min flat.





Luxiurous as fuck! :datass:


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InvisibleRepertoire89
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Re: An average footlong at subway is unhealthy as fuck [Re: LogicaL Chaos] * 1
    #23687327 - 09/28/16 03:59 PM (7 years, 4 months ago)

It was great man, I genuinely regret leaving that place

Had a good fucking woman out there, a job with all kinds of opportunities
Living on the beach, working on the beach, commuting on the beach

$950 2bdrm near Point Loma, fucking great deal for the location

:blowmybrainsout:


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Invisiblepirate-blues
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Re: An average footlong at subway is unhealthy as fuck [Re: Repertoire89]
    #23687463 - 09/28/16 04:30 PM (7 years, 4 months ago)

Dude, that price for a 2 bedroom on the beach in San Diego?!?!

that's awesome dude...I actually had no idea that was even possible(I've got family out in SD, lovely, lovely place) , but hey, sometimes life calls, other deals will be had and if you wanted to be back at that place(not that place specifically, but in SD) badly enough, you could be. But hey, the future holds many things if you play your cards right and in them are a place in your life you'll love even more :cheers:


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InvisibleBigfeely123
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Re: An average footlong at subway is unhealthy as fuck [Re: Repertoire89]
    #23687470 - 09/28/16 04:32 PM (7 years, 4 months ago)

Were you in the ghetto


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Invisiblepirate-blues
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Re: An average footlong at subway is unhealthy as fuck [Re: Bigfeely123]
    #23687497 - 09/28/16 04:41 PM (7 years, 4 months ago)

He said it was on the beach, so probably not but I would live in the ghetto in san diego for that price if it was on the beach.

I feel like the definition of ghetto in San Diego differs from that of Philly's definition.


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InvisiblePrisoner#1
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Re: An average footlong at subway is unhealthy as fuck [Re: Crystal G] * 1
    #23687710 - 09/28/16 05:47 PM (7 years, 4 months ago)

Quote:

Crystal G said:
When I was living in the Mexican neighborhood  I lived right next door to this taco shop. They were literally my next door neighbors that renovated their home to turn it into a walk in business.

I always ordered the tripe, tongue, and beef cheek tacos from them. 75 cents a taco, and unbelievably on taco Tuesdays the price gets even cheaper,it drops down to like 62 cents or something.

Then there's another places where tacos are a dollar but they slice meat fresh off the adobada rotating meat revolver like this







my son stepped out of his comfort zone and tried the tongue, he's not brave enough for tripe yet and he doesnt even know what barbacoa is, I just tell him to give it a shot


I want tacos but I'm roasting some beast


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InvisiblePrisoner#1
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Re: An average footlong at subway is unhealthy as fuck [Re: musiclover420] * 2
    #23687714 - 09/28/16 05:48 PM (7 years, 4 months ago)

Quote:

musiclover420 said:
Quote:

Prisoner#1 said:
hope your head doesnt explode when you learn that
aspartame is non GMO and gluten free ya hipster





Yes and it is also made from genetically modified E Coli :lol:

Aspartame patent

Quote:

The artificial sweetener aspartame, a dipeptide with the formula Asp-Phe-me, is produced using a cloned microorganism.




Quote:

A microorganism according to claim 14 wherein said microorganism is a strain of E.coli K12.








cloning isnt genetic modification, it's considered a traditional breeding method


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Offlinemusiclover420
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Re: An average footlong at subway is unhealthy as fuck [Re: Prisoner#1]
    #23687769 - 09/28/16 06:06 PM (7 years, 4 months ago)

Quote:





Strain Information

E. coli K-12 MG1655

Description

Genotype: F- lambda- ilvG- rfb-50 rph-1

Serotype: OR:H48:K-

This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation




Just becouse it has had "minimal genetic manipulation" doesn't meant they haven't modified the genetics which is AKA being genetically modified :lol:


--------------------
Don't worry about me, I've got all that I need. And I'm singing my song to the sky

You know how it feels, With the breeze of the sun in your eyes. Not minding that time's passing by

I've got all and more, My smile, just as before. Is all that I carry with me

I talk to myself, I need nobody else. I'm lost and I'm mine, yes I'm free



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InvisiblePrisoner#1
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Re: An average footlong at subway is unhealthy as fuck [Re: musiclover420]
    #23687777 - 09/28/16 06:08 PM (7 years, 4 months ago)

just because you want to read it as geneticallt modified doesnt mean it is, claiming
something is non-GMO is illegal if it is genetically modified


which strain of e.coli was this non-gmo aspartame made from?


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Invisiblepsi
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Re: An average footlong at subway is unhealthy as fuck [Re: Prisoner#1]
    #23687801 - 09/28/16 06:14 PM (7 years, 4 months ago)

Quote:

Prisoner#1 said:
cloning isnt genetic modification, it's considered a traditional breeding method



Try reading some of the patent page he linked, what they describe there is in fact genetic modification.

Quote:

DNA segments suitable for cloning are obtained from other organisms or must be synthesized. As there is no known natural source for a DNA strand which codes for the repeating protein sequence Asp-Phe, an appropriate DNA chain must be synthesized.




Quote:

A method for producing aspartame, comprising, synthesizing double stranded DNA in which a coding strand has alternating codons for Asp and Phe, said codons being of a number sufficient to produce a polypeptide which is stable in a predetermined host microorganism, insertinq said DNA strand into a cloning vehicle so that resulting chimera directs the synthesis of said protein, introducing said chimera into said predetermined host microorganism and cultivating said microorganism to produce said stable polypeptide, harvestinq said stable polypeptide from said host microorganism esterifying the free carboxyl group of aspartic acid by benzylation, fragmenting said polypeptide to produce benzyl-Asp-Phe dipeptides, methylating the carboxyl group of the Phe moiety, and debenzylating the aspartic acid carboxyl group by hydrogenolysis.




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Offlinemusiclover420
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Re: An average footlong at subway is unhealthy as fuck [Re: Prisoner#1]
    #23687802 - 09/28/16 06:14 PM (7 years, 4 months ago)

Quote:

Prisoner#1 said:
which strain of e.coli was this non-gmo aspartame made from?




It wasn't made from one it was isolated and then modified in the lab... That doesn't make it a new strain that just means they took an existing natural strain and then modified it... Even if they modified it in the slightest way that still makes the genetics of it modifies :lol: I never said it was extremely modified into something new...

Quote:

claiming
something is non-GMO is illegal if it is genetically modified




Where does anyone claim it was not modified?

I posted a link to the patent where you can read the specifics, you are essentially refuting fact until you post some sources...


--------------------
Don't worry about me, I've got all that I need. And I'm singing my song to the sky

You know how it feels, With the breeze of the sun in your eyes. Not minding that time's passing by

I've got all and more, My smile, just as before. Is all that I carry with me

I talk to myself, I need nobody else. I'm lost and I'm mine, yes I'm free



Extras: Filter Print Post Top
Invisiblepsi
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Re: An average footlong at subway is unhealthy as fuck [Re: musiclover420]
    #23687905 - 09/28/16 06:40 PM (7 years, 4 months ago)

The method outlined in that patent unquestionably involves genetic modification, but it may still not be the case that much or any aspartame commercially produced today is made in that way. The WP article on aspartame mentions two main commercial methods, one which sounds like a pretty traditional chemical synthesis, and another that uses an enzyme extracted from Bacillus thermoproteolyticus.


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Offlinemusiclover420
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Re: An average footlong at subway is unhealthy as fuck [Re: psi]
    #23688060 - 09/28/16 07:23 PM (7 years, 4 months ago)

Quote:

psi said:
but it may still not be the case that much or any aspartame commercially produced today is made in that way. The WP article on aspartame mentions two main commercial methods, one which sounds like a pretty traditional chemical synthesis, and another that uses an enzyme extracted from Bacillus thermoproteolyticus.




Yeah I saw that, I mainly just think its crazy that aspartame came about that way in general.

It makes sense they would find a more efficient way to synth it though :shrug:


--------------------
Don't worry about me, I've got all that I need. And I'm singing my song to the sky

You know how it feels, With the breeze of the sun in your eyes. Not minding that time's passing by

I've got all and more, My smile, just as before. Is all that I carry with me

I talk to myself, I need nobody else. I'm lost and I'm mine, yes I'm free



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InvisiblePrisoner#1
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Re: An average footlong at subway is unhealthy as fuck [Re: psi]
    #23688107 - 09/28/16 07:33 PM (7 years, 4 months ago)

Quote:

psi said:
The method outlined in that patent unquestionably involves genetic modification






explain how it was genetically modified?


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InvisiblePrisoner#1
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Re: An average footlong at subway is unhealthy as fuck [Re: musiclover420]
    #23688121 - 09/28/16 07:35 PM (7 years, 4 months ago)

Quote:

musiclover420 said:
Quote:

Prisoner#1 said:
which strain of e.coli was this non-gmo aspartame made from?




It wasn't made from one it was isolated and then modified in the lab... That doesn't make it a new strain that just means they took an existing natural strain and then modified it... Even if they modified it in the slightest way that still makes the genetics of it modifies :lol: I never said it was extremely modified into something new...




and exactly how was it modified?

Quote:

Quote:

claiming
something is non-GMO is illegal if it is genetically modified




Where does anyone claim it was not modified?







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Offlinemusiclover420
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Re: An average footlong at subway is unhealthy as fuck [Re: Prisoner#1]
    #23688177 - 09/28/16 07:47 PM (7 years, 4 months ago)

Quote:

Prisoner#1 said:

and exactly how was it modified?





How about you read the sources I provided :lol: or provide you own...


--------------------
Don't worry about me, I've got all that I need. And I'm singing my song to the sky

You know how it feels, With the breeze of the sun in your eyes. Not minding that time's passing by

I've got all and more, My smile, just as before. Is all that I carry with me

I talk to myself, I need nobody else. I'm lost and I'm mine, yes I'm free



Extras: Filter Print Post Top
Invisiblepsi
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Re: An average footlong at subway is unhealthy as fuck [Re: Prisoner#1]
    #23688205 - 09/28/16 07:51 PM (7 years, 4 months ago)

Quote:

Prisoner#1 said:
Quote:

psi said:
The method outlined in that patent unquestionably involves genetic modification






explain how it was genetically modified?



I underlined the parts in the quotes where they explain it. A DNA sequence that codes for those two amino acids over and over again had to be made made synthetically, then introduced into the target organism via a "cloning vehicle" that I don't think they specified.


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