Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineSN71i
Stranger


Registered: 10/01/15
Posts: 4
Last seen: 7 years, 3 months
Odd growth on oyster mushroom agar?
    #23682477 - 09/27/16 08:29 AM (7 years, 4 months ago)

Hey, so three days ago I inoculated 8 agar jars with 1 drop of MS solution (in a SAB), using the agar recipe from Pastywhyte's tek.

Now one out of the eight jars has what looks to be some mycelial growth and the other 7 have this wired growth I've never seen.

This is my first time working with agar and with oyster mushrooms so any input would be greatly appreciated. 

These are what has happened to the 7 of 8 jars.






This (although hard to see) is the 1 of 8 jars with what looks to be mycelium.
<--- gotta take notes :wink:

Have any of you seen this before?


Extras: Filter Print Post Top
Offlineadadada
Stranger
 User Gallery

Registered: 01/18/16
Posts: 101
Last seen: 5 years, 9 months
Re: Odd growth on oyster mushroom agar? [Re: SN71i]
    #23682539 - 09/27/16 08:55 AM (7 years, 4 months ago)

I've seen it before and assumed it was contamination and threw it out.

I think this should be moved to the contamination forum.


Extras: Filter Print Post Top
OfflineJanuaryWolf
JW
Female User Gallery


Registered: 01/04/15
Posts: 63
Loc: North Texas
Last seen: 5 years, 7 months
Re: Odd growth on oyster mushroom agar? [Re: adadada]
    #23684076 - 09/27/16 06:24 PM (7 years, 4 months ago)

Quote:

adadada said:
I've seen it before and assumed it was contamination and threw it out.





Same.


Extras: Filter Print Post Top
Offlineanthiawe
friendly stranger
 User Gallery

Registered: 05/18/16
Posts: 652
Last seen: 2 years, 7 months
Re: Odd growth on oyster mushroom agar? [Re: JanuaryWolf]
    #23684154 - 09/27/16 06:43 PM (7 years, 4 months ago)

its bacterial contamination. what is MS solution? and do you use 70% isoproyl?


--------------------
TEK compendium


Extras: Filter Print Post Top
OfflineSN71i
Stranger


Registered: 10/01/15
Posts: 4
Last seen: 7 years, 3 months
Re: Odd growth on oyster mushroom agar? [Re: anthiawe]
    #23684432 - 09/27/16 08:13 PM (7 years, 4 months ago)

Quote:

anthiawe said:
its bacterial contamination. what is MS solution? and do you use 70% isoproyl?



MS solution = multi spore syringe :syringe:
I used 99% isopropyl alcohol.  With that said instead of a synthetic disk filter or micropore tape, my jars were covered with 2 layers of  paper towel (<--definitely what caused it). And I did not flame sterilize the needle before each drop of solution was dropped.

Im thinking about using thick felt to cover the holes on the top of the agar jars next time.  :shrug:


Extras: Filter Print Post Top
OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: Odd growth on oyster mushroom agar? [Re: JanuaryWolf]
    #23684874 - 09/27/16 10:48 PM (7 years, 4 months ago)

Quote:

JanuaryWolf said:
Quote:

adadada said:
I've seen it before and assumed it was contamination and threw it out.





Same.




Ditto


--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


Extras: Filter Print Post Top
OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: Odd growth on oyster mushroom agar? [Re: SN71i]
    #23684884 - 09/27/16 10:50 PM (7 years, 4 months ago)

Quote:

SN71i said:
Quote:

anthiawe said:
its bacterial contamination. what is MS solution? and do you use 70% isoproyl?



MS solution = multi spore syringe :syringe:
I used 99% isopropyl alcohol.  With that said instead of a synthetic disk filter or micropore tape, my jars were covered with 2 layers of  paper towel (<--definitely what caused it). And I did not flame sterilize the needle before each drop of solution was dropped.

Im thinking about using thick felt to cover the holes on the top of the agar jars next time.  :shrug:




70% isopropyl is actually better for sterilizing


--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


Extras: Filter Print Post Top
InvisibleRaven44
Entry not permitted to muggles
Male


Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
Re: Odd growth on oyster mushroom agar? [Re: TravelAgency]
    #23684939 - 09/27/16 11:10 PM (7 years, 4 months ago)

Sanitizing not sterilizing


Extras: Filter Print Post Top
OfflineTravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
Re: Odd growth on oyster mushroom agar? [Re: Raven44]
    #23684971 - 09/27/16 11:31 PM (7 years, 4 months ago)

You are correct, splitting hairs nontheless- when you create a sterile work environment- you use 70% isopropyl- think SAB use.


--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


Extras: Filter Print Post Top
InvisibleRaven44
Entry not permitted to muggles
Male


Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
Re: Odd growth on oyster mushroom agar? [Re: TravelAgency]
    #23684998 - 09/27/16 11:45 PM (7 years, 4 months ago)

There's a big difference actually in my book

For instance, bleach and alchohol have the power to sanitize to certain degrees. On the contrary ozone  (O3) has the power to sanitize. Most things do not have the power to sterilize by far.

70% alch  is commonly used in clean work. SAB ,flow hood ect. I do believe u r correct as well on this

I wouldn't say either of these creates a "sterile" work environment quite. That's once again misuse of the word sterile and sterilizes :wink:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* oyster mushrooms are easy to grow Mitchnast 6,588 7 05/30/01 09:54 PM
by Mitchnast
* Oyster Mushrooms felix 3,163 2 03/09/02 10:36 PM
by rungi
* Oyster Mushroom Substrates??? TrippinRhino 7,502 8 11/01/01 07:05 AM
by NDK
* Is it possible to cultivate oyster mushrooms using cakes? Wheedhead! 5,245 8 04/24/14 08:40 AM
by tiltajoel
* oyster mushroom and ??HELP QUICK dimitri211 2,397 3 08/04/01 04:12 PM
by 40oz
* Growing oyster mushrooms on straw AnnoA 6,696 15 05/13/17 02:08 PM
by flyontoast
* Post deleted by Administrator Anonymous 1,730 7 09/09/03 04:41 PM
by THATS iT!
* Oyster Mushrooms MattyB 2,469 4 07/18/01 08:54 AM
by MattyB

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
374 topic views. 0 members, 8 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 14 queries.