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SN71i
Stranger


Registered: 10/01/15
Posts: 4
Last seen: 7 years, 3 months
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Odd growth on oyster mushroom agar?
#23682477 - 09/27/16 08:29 AM (7 years, 4 months ago) |
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Hey, so three days ago I inoculated 8 agar jars with 1 drop of MS solution (in a SAB), using the agar recipe from Pastywhyte's tek.
Now one out of the eight jars has what looks to be some mycelial growth and the other 7 have this wired growth I've never seen.
This is my first time working with agar and with oyster mushrooms so any input would be greatly appreciated.
These are what has happened to the 7 of 8 jars.



This (although hard to see) is the 1 of 8 jars with what looks to be mycelium.
<--- gotta take notes 
Have any of you seen this before?
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adadada
Stranger


Registered: 01/18/16
Posts: 101
Last seen: 5 years, 9 months
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Re: Odd growth on oyster mushroom agar? [Re: SN71i]
#23682539 - 09/27/16 08:55 AM (7 years, 4 months ago) |
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I've seen it before and assumed it was contamination and threw it out.
I think this should be moved to the contamination forum.
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JanuaryWolf
JW



Registered: 01/04/15
Posts: 63
Loc: North Texas
Last seen: 5 years, 7 months
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Re: Odd growth on oyster mushroom agar? [Re: adadada]
#23684076 - 09/27/16 06:24 PM (7 years, 4 months ago) |
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Quote:
adadada said: I've seen it before and assumed it was contamination and threw it out.
Same.
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anthiawe
friendly stranger


Registered: 05/18/16
Posts: 652
Last seen: 2 years, 7 months
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Re: Odd growth on oyster mushroom agar? [Re: JanuaryWolf]
#23684154 - 09/27/16 06:43 PM (7 years, 4 months ago) |
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its bacterial contamination. what is MS solution? and do you use 70% isoproyl?
-------------------- TEK compendium
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SN71i
Stranger


Registered: 10/01/15
Posts: 4
Last seen: 7 years, 3 months
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Re: Odd growth on oyster mushroom agar? [Re: anthiawe]
#23684432 - 09/27/16 08:13 PM (7 years, 4 months ago) |
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Quote:
anthiawe said: its bacterial contamination. what is MS solution? and do you use 70% isoproyl?
MS solution = multi spore syringe  I used 99% isopropyl alcohol. With that said instead of a synthetic disk filter or micropore tape, my jars were covered with 2 layers of paper towel (<--definitely what caused it). And I did not flame sterilize the needle before each drop of solution was dropped.
Im thinking about using thick felt to cover the holes on the top of the agar jars next time.
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
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Re: Odd growth on oyster mushroom agar? [Re: JanuaryWolf]
#23684874 - 09/27/16 10:48 PM (7 years, 4 months ago) |
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Quote:
JanuaryWolf said:
Quote:
adadada said: I've seen it before and assumed it was contamination and threw it out.
Same.
Ditto
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
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Re: Odd growth on oyster mushroom agar? [Re: SN71i]
#23684884 - 09/27/16 10:50 PM (7 years, 4 months ago) |
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Quote:
SN71i said:
Quote:
anthiawe said: its bacterial contamination. what is MS solution? and do you use 70% isoproyl?
MS solution = multi spore syringe  I used 99% isopropyl alcohol. With that said instead of a synthetic disk filter or micropore tape, my jars were covered with 2 layers of paper towel (<--definitely what caused it). And I did not flame sterilize the needle before each drop of solution was dropped.
Im thinking about using thick felt to cover the holes on the top of the agar jars next time. 
70% isopropyl is actually better for sterilizing
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Re: Odd growth on oyster mushroom agar? [Re: TravelAgency]
#23684939 - 09/27/16 11:10 PM (7 years, 4 months ago) |
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Sanitizing not sterilizing
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
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Re: Odd growth on oyster mushroom agar? [Re: Raven44]
#23684971 - 09/27/16 11:31 PM (7 years, 4 months ago) |
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You are correct, splitting hairs nontheless- when you create a sterile work environment- you use 70% isopropyl- think SAB use.
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Re: Odd growth on oyster mushroom agar? [Re: TravelAgency]
#23684998 - 09/27/16 11:45 PM (7 years, 4 months ago) |
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There's a big difference actually in my book
For instance, bleach and alchohol have the power to sanitize to certain degrees. On the contrary ozone (O3) has the power to sanitize. Most things do not have the power to sterilize by far.
70% alch is commonly used in clean work. SAB ,flow hood ect. I do believe u r correct as well on this
I wouldn't say either of these creates a "sterile" work environment quite. That's once again misuse of the word sterile and sterilizes
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