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kaius711
Wandering Entity
Registered: 09/15/16
Posts: 63
Last seen: 6 years, 7 months
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doc34 agar tek question
#23682343 - 09/27/16 07:15 AM (8 years, 11 days ago) |
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https://www.shroomery.org/8514/Agar-substitute greetings. this tek says i have to use 5 grams of ground up brown rice however my question is that would 5 grams of brown rice flour do the job equally. thank you for your assistance.
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Shineonyoucrazy
Apprentice fungi
Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
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Re: doc34 agar tek question [Re: kaius711]
#23682751 - 09/27/16 10:17 AM (8 years, 11 days ago) |
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I just used this same tek and substituted brf for rice and it worked fine. Make sure while your bring your pc up to temp to do it slow and steady. This will keep it from going up the side of your plates. Good luck!
-------------------- Keep your feet on the ground and your head in the clouds
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kaius711
Wandering Entity
Registered: 09/15/16
Posts: 63
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Thank you good sir
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Shineonyoucrazy
Apprentice fungi
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Re: doc34 agar tek question [Re: kaius711]
#23684596 - 09/27/16 09:09 PM (8 years, 10 days ago) |
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No problem! Check out pastyplates easy no pour agar. If you've got the pc, like $10- $15 is all the materials cost for it and the plates seem like better quality.
-------------------- Keep your feet on the ground and your head in the clouds
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tombosley8
Full on... Bossley Baggins
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Psilosoulful
Registered: 09/05/14
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Re: doc34 agar tek question [Re: tombosley8]
#23685061 - 09/28/16 12:23 AM (8 years, 10 days ago) |
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Yea I'd definitely just go right to pasty's tek
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morty422
Wuba-luba-dub-dub!
Registered: 07/06/16
Posts: 988
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Quote:
Psilosoulful said: Yea I'd definitely just go right to pasty's tek
Pastywhyte's tek is the way to go if your just getting into agar.
The ability to take a fully colonized plate and "tic-tac-toe" (Tiger drop 2.0) and plop it into your master jar feels too good.
I have a BUNCH of actual agar dishes and I still use PP5's exclusively to drop my masters. It's just too easy...
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Psilosoulful
Registered: 09/05/14
Posts: 7,205
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Re: doc34 agar tek question [Re: morty422]
#23685097 - 09/28/16 12:40 AM (8 years, 10 days ago) |
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,901
Loc: Milky way
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Last link in signature is an amalgamation of the good agar resources that are up to date here
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filthyknees
no coincidence
Registered: 03/08/13
Posts: 6,283
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Re: doc34 agar tek question [Re: morty422]
#23685543 - 09/28/16 06:38 AM (8 years, 10 days ago) |
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Quote:
morty422 said: I have a BUNCH of actual agar dishes and I still use PP5's exclusively to drop my masters. It's just too easy...
When you have a bunch of Petri dishes made for exactly what we need but choose to use pp5 containers.
I have to say I have never been in that boat. Pp5 are functional but there is no comparing to actual petris.....
Idk why no pour is considered easier and 'better' for noobs, maybe cause it's repeated over and over, the two methods are both very, very easy...
What's too easy about pp5s? Fitling with the lid that sticks sometimes? Having like ten take up a lot of space? View your growth through plastic that's not fully clear. I fail to see how it is too easy. And I've done pp5s maybe a hundred times and done thousands of pour pertis..
There are zero commercial operations using pp5 glad rounds as petris
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Shineonyoucrazy
Apprentice fungi
Registered: 09/22/16
Posts: 917
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I don't think the comparison is necessarily which is easier, but rather what is accessible, very cheap ( maybe free depending on what you have laying around the house), and depending on your situation it could be more stealthy. Pastyplates are a springboard into agar. I'm making an assumption based off him (the op) looking at the doc tek, but I'm guess is probably a cheap into to see what agar is all about. I know that's why i started with docs, then to pastyplates.
-------------------- Keep your feet on the ground and your head in the clouds
Edited by Shineonyoucrazy (09/28/16 07:43 AM)
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morty422
Wuba-luba-dub-dub!
Registered: 07/06/16
Posts: 988
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Quote:
filthyknees said: What's too easy about pp5s?
I love the ability to cut them, turn them over, and drop them by lightly pressing the sides in/bottom up. I can do it in a few seconds and it's extremely easy for me.
Quote:
filthyknees said: Fitling with the lid that sticks sometimes?
I've never really had an issue with the lids.
They always open up really easy for me.
Quote:
filthyknees said: Having like ten take up a lot of space?
When I started with agar, they did take up WAY too much space... However, I only use them for that last transfer before agar>grain now - so I never really have 10 at a time in my SAB. I have maybe 5 at the most.
Quote:
filthyknees said: View your growth through plastic that's not fully clear. I fail to see how it is too easy. And I've done pp5s maybe a hundred times and done thousands of pour pertis..
I see your points here - but by the time I am using them, the mycelium is clean 99% of the time. The "too easy" part is the drop into the grain jar. I can create 10/15/20 pieces and drop them all into the jar in one fluid motion. It only takes me a few seconds. I like the idea behind having a malleable vessel in the end-I make far less mistakes with the media and it feels more familiar to me.
It's personal preference for me... I still use petris for new growth/clones/etc... but when it comes to the drop, I love using PP5.
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mrmazdarx9
Pffffttt
Registered: 05/15/16
Posts: 9,796
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Last seen: 2 years, 11 days
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Quote:
filthyknees said:
Quote:
morty422 said: I have a BUNCH of actual agar dishes and I still use PP5's exclusively to drop my masters. It's just too easy...
When you have a bunch of Petri dishes made for exactly what we need but choose to use pp5 containers.
I have to say I have never been in that boat. Pp5 are functional but there is no comparing to actual petris.....
Idk why no pour is considered easier and 'better' for noobs, maybe cause it's repeated over and over, the two methods are both very, very easy...
What's too easy about pp5s? Fitling with the lid that sticks sometimes? Having like ten take up a lot of space? View your growth through plastic that's not fully clear. I fail to see how it is too easy. And I've done pp5s maybe a hundred times and done thousands of pour pertis..
There are zero commercial operations using pp5 glad rounds as petris
I love petris and its not a bad thing to be able to pour dishes early on in when using agar
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Greg
always learning
Registered: 10/28/15
Posts: 1,536
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Re: doc34 agar tek question [Re: mrmazdarx9]
#23686559 - 09/28/16 12:56 PM (8 years, 10 days ago) |
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Before I started pouring I tried no-pour and had a series of problems with them. Not saying they are inherently bad, but I don't like using anything other than disposable petris. I used to be really paranoid that poured agar would contaminate really bad. Not sure why I thought that. Out of the 300+ dishes I've poured, only 1 has contaminated before I used it. I don't store my dishes in the fridge, I just leave them in my SAB until they're all used up.
I don't do the tiger drop though, I just cut a ton of squares out of a petri and use one square per grain jar. I don't even G2G
With no-pours, visibility was a huge issue too. I hate the idea of having a filter on my dishes also, just one more thing that can fail and let nasties in.
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morty422
Wuba-luba-dub-dub!
Registered: 07/06/16
Posts: 988
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Re: doc34 agar tek question [Re: Greg]
#23686751 - 09/28/16 01:41 PM (8 years, 10 days ago) |
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Quote:
Greg said: Before I started pouring I tried no-pour and had a series of problems with them. Not saying they are inherently bad, but I don't like using anything other than disposable petris. I used to be really paranoid that poured agar would contaminate really bad. Not sure why I thought that. Out of the 300+ dishes I've poured, only 1 has contaminated before I used it. I don't store my dishes in the fridge, I just leave them in my SAB until they're all used up.
I don't do the tiger drop though, I just cut a ton of squares out of a petri and use one square per grain jar. I don't even G2G
With no-pours, visibility was a huge issue too. I hate the idea of having a filter on my dishes also, just one more thing that can fail and let nasties in.
Good points - especially when you spoke about the filter being another variable.
I think what works best for you and gives you the best outcome is what you should use.
Maybe in the future I will use nothing but petris - but for now, I can't get rid of my pasty plates yet... hehe
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