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InvisibleAlmostBlue
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Rye jars driving a biologist crazy *DELETED*
    #23682142 - 09/27/16 04:03 AM (7 years, 4 months ago)

Post deleted by AlmostBlue

Reason for deletion: not useful



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Invisibleblindingleaf
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682175 - 09/27/16 04:43 AM (7 years, 4 months ago)

Quote:

i'm a biologist and know lots about mushrooms and microbiology in general.





Quote:

In my opinion someone of my family found the jars, got curious and opened them, i can see no other explaination for these contams!




as someone who has a biology background,  this is your first instinct…?

what are they teaching you in school?!?!?

those grains, even before shaking, looking a little off.  may have been MS syringe (bacteria) or filter failure (mold)


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MICROBIAL HUSBANDRY!!!!

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InvisibleInocuole
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
    #23682191 - 09/27/16 04:55 AM (7 years, 4 months ago)

If you wanted to show up your friend with a kit, inoculating grain with a a spore syringe was not the way to go about it.


--------------------
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Offlinemrmazdarx9
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682193 - 09/27/16 04:59 AM (7 years, 4 months ago)

Quote:

Inocuole said:
If you wanted to show up your friend with a kit, inoculating grain with a a spore syringe was not the way to go about it.



:whathesaid: as a biologist who's worked with agar you must know spore syringes are full of crap that's why you used agar.


--------------------
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InvisibleAlmostBlue
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
    #23682199 - 09/27/16 05:03 AM (7 years, 4 months ago)

Hey nerdy boy keep calm. Looks like you're not the only mushroom grower out there.

I say this because i'm pretty sure my filter system didn't fail as it was STERILE and SUPER TIGHT.

Also my siringe was ordered from the internet, and flame sterilized the needle every time in and out.

I've been out of town for 4 days and when I left it looked super healty that's why i think someone opened it while i was out.

Still you are a 'trusted cultivator' but didn't reply to my simple questions.

If you are on your period just leave my thread, thanks


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InvisibleAlmostBlue
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682214 - 09/27/16 05:20 AM (7 years, 4 months ago)

Quote:

Inocuole said:
If you wanted to show up your friend with a kit, inoculating grain with a a spore syringe was not the way to go about it.




yeah well i wanted to spawn the grain and colonize a big monotub, what's the right way to go about it?

ONLY RELEVANT REPLIES PLEASE, OTHERWISE JUST GO ON PORNHUB YOU'LL FEEL MORE CONFIDENT THEN


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OfflineTheLongComma
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682215 - 09/27/16 05:21 AM (7 years, 4 months ago)

Wouldn't matter if your needle was sterile if the spores inside were dirty to begin with.

Also, you said you micropored the hole after inoculation? Does that mean you took the polyfill out to do that?


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Invisibleblindingleaf
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682217 - 09/27/16 05:22 AM (7 years, 4 months ago)

will I feel as confident at porn hub as you did with your "internet syringes" and"sterile tight" filter?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleInocuole
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Re: Rye jars driving a biologist crazy [Re: TheLongComma]
    #23682218 - 09/27/16 05:24 AM (7 years, 4 months ago)

I guess this guy doesn't want help with his bacterial jar.. not that there's any helping it.  You either use agar to ensure you have a clean culture or you can keep guessing between inoculate or filter fail until something gives...  In case BLs sarcasm wasn't laid on thick enough "internet syringes" are just as likely to harbor bacteria as anything else.


--------------------
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InvisibleAlmostBlue
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
    #23682238 - 09/27/16 05:59 AM (7 years, 4 months ago)

Well, I felt like doing agar was not necessary as the syringe provider (inner visions) said the spore syringe was contam free, and i had no interest in selecting genetics, and little time to do the whole thing.

Opening a sterile jar to insert an agar slice isn't safer than inoculating from a hole if you assume your syringes are contam-free!

Never took out the polyfill, taped the outside after inoculation just to be safer.

Your sarcasm is totally pointless and just shows me how twisted and sick you guys are. Just give your opinion, not stupid pointless critiques just because you feel 'experts'.

I previously realized a 3d carboard puzzle model of an ancient building, sterilized, filled it with substrate and spawn, let it colonize and managed to fruit beautiful mushrooms from the 'windows' of the building. and i was 18yo at the time.

As I said, you are not the only mushroom growers out there. It's pretty easy actually! So stop acting like pussies and tell me if it's worth fruiting the unshaken jars with a casing layer or just as cakes rolled in verm, which was the ONLY question i've asked so far.


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Offlinenuentoter
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682249 - 09/27/16 06:06 AM (7 years, 4 months ago)

Lesson learned, toss the junk, take a sliver of your white mycelium from your best looking grain and isolate on agar. Then go back to some clean grains with new filters.


--------------------

The geometry of us is no chance. We are antennae, we are tuning forks, we are receiver and transmitters of all energy. We are more than we know.  - @entheolove

"I found I could say things with color and shapes that I couldn't say any other way - things I had no words for"  - Georgia O'Keefe

I think the word is vagina


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Offlinemrmazdarx9
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Re: Rye jars driving a biologist crazy [Re: nuentoter]
    #23682260 - 09/27/16 06:19 AM (7 years, 4 months ago)

Oh dear this is going well, this all smells of b.s. any biologist knows the basics and internet syringes are not clean

Why don't you stop being a pussy and utfse and figure it out yourself


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Edited by mrmazdarx9 (09/27/16 06:24 AM)


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Invisibleblindingleaf
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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
    #23682270 - 09/27/16 06:28 AM (7 years, 4 months ago)

just throw some coir on top, keep it in the jar, and see if it works for crying out loud.

jesus christ, if u made fucking 3D oyster puzzles (when u were 18 y/o no less:rolleyes: ), i'd think u'd have at least SOME idea of how to proceed with those jars.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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InvisibleAlmostBlue
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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
    #23682273 - 09/27/16 06:30 AM (7 years, 4 months ago)

Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great? :crazy2:

Now everybody saying that internet spores are junk, while lots of guides from trusted cultivators make use of spore syringes not mentioning this problem.

This forum's a real bitch!


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Offlinemrmazdarx9
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682276 - 09/27/16 06:32 AM (7 years, 4 months ago)

Bye then:highfive:


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Invisiblefilthyknees
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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
    #23682277 - 09/27/16 06:32 AM (7 years, 4 months ago)

"Your not the only mushroom grower, it's not that hard!!!! Now please help me!!!' :lol:

They already did. Start over: use agar, make a few transfers and innoculations your jar with clean spawn. First pull the stick from yer bunghole

You haven't gotten a single fruit so how can you say the other stuff is going great


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
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Invisibleblindingleaf
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Re: Rye jars driving a biologist crazy [Re: filthyknees]
    #23682281 - 09/27/16 06:34 AM (7 years, 4 months ago)

yes, we're the worst. have a nice day:cool:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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InvisibleInocuole
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Re: Rye jars driving a biologist crazy [Re: nuentoter]
    #23682298 - 09/27/16 06:44 AM (7 years, 4 months ago)

Quote:

nuentoter said:
Lesson learned, toss the junk, take a sliver of your white mycelium from your best looking grain and isolate on agar. Then go back to some clean grains with new filters.




I wouldn't bother trying to save that mycelium in particular when he could just start from spores again and maybe have a better chance of isolating something clean straight away.  If mold gets a chance to interlace with a culture, then it's difficult/near impossible to separate them.  Bacteria's a little easier but still a pain.

Quote:

AlmostBlue said:
Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great? :crazy2:





Made LC with spores, thinks it's doing great.  :rofl:

I figured you could take it bluntly since you were a biologist but I guess you're one of those experts... the ones that already know everything and dismiss good advice while flaunting their mistakes as if they're marks of success.  I didn't anticipate you getting your feelings hurt though.  Quite unfortunate.

If you want to know why people were short with you to begin with, it's because you said this
Quote:

Well now I wanted to challenge a friend of mine, all proud with his cambodian mycelium kit, wanted to show him how to do a proper grow as i'm a biologist and know lots about mushrooms and microbiology in general.



And then proceeded to list off one noob thing after another, like you may as well have never heard of mushrooms at all prior to this.  A proper grow goes a lot smoother with a proper mindset, and I don't think anybody here is seeing that you have that..


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Edited by Inocuole (09/27/16 06:49 AM)


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Invisibleblindingleaf
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682308 - 09/27/16 06:51 AM (7 years, 4 months ago)

everything:whathesaid: 100%


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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InvisibleAlmostBlue
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
    #23682310 - 09/27/16 06:52 AM (7 years, 4 months ago)

Of course i have some ideas (...) actually i'm asking what to do cause here i can find people with more experience with these techniques and if some real expert says i'm just gonna lose my time i'd toss everything away and go fuck myself.

My major experience is in molecular biology, things you can't really do at home and probably the most of you don't understand but i'm not bitching around cause i have genetic engineering skills, which i'm more likely to share with a open community than use to make fun of others!

I'll try anyway, some cased with coir and some rolled in verm and set in fruiting chamber

What a bunch of psychos i was just asking a REALLY SIMPLE question, and still the most of you just wrote something else to make fun of me (how poor you are), not really reading my first post about trying to save unshaken jars rather than starting the whole process with new grains due to no time. Read-->Understand-->Reply is a hard protocol nowdays


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Invisibleblindingleaf
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682315 - 09/27/16 06:56 AM (7 years, 4 months ago)

a little humility and a sense of humor go a long way.

ur post could have just been the pictures and a question.  that would have been a "really simple" way to get a "read>understand>reply" response.

don't take us as seriously as u take urself dude.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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InvisibleAlmostBlue
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682354 - 09/27/16 07:22 AM (7 years, 4 months ago)

Quote:

Inocuole said:
Quote:

nuentoter said:
Lesson learned, toss the junk, take a sliver of your white mycelium from your best looking grain and isolate on agar. Then go back to some clean grains with new filters.




I wouldn't bother trying to save that mycelium in particular when he could just start from spores again and maybe have a better chance of isolating something clean straight away.  If mold gets a chance to interlace with a culture, then it's difficult/near impossible to separate them.  Bacteria's a little easier but still a pain.

Quote:

AlmostBlue said:
Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great? :crazy2:





Made LC with spores, thinks it's doing great.  :rofl:

I figured you could take it bluntly since you were a biologist but I guess you're one of those experts... the ones that already know everything and dismiss good advice while flaunting their mistakes as if they're marks of success.  I didn't anticipate you getting your feelings hurt though.  Quite unfortunate.

If you want to know why people were short with you to begin with, it's because you said this
Quote:

Well now I wanted to challenge a friend of mine, all proud with his cambodian mycelium kit, wanted to show him how to do a proper grow as i'm a biologist and know lots about mushrooms and microbiology in general.



And then proceeded to list off one noob thing after another, like you may as well have never heard of mushrooms at all prior to this.  A proper grow goes a lot smoother with a proper mindset, and I don't think anybody here is seeing that you have that..





lol what's weird about an LC from spores? Using mycelium tissue is of course faster but do you know spores can properly germinate and have sex in a LC?

But all this is related to the fact I said I'm a (molecular)biologist so you guys growing mushrooms at your place since who knows when felt like you had to give me a lesson. Well there's no lesson to give my friends, i just asked 2 very simple questions in the first post. Almost none of you replied with something useful, just bitching about how experienced in growing mushrooms you are. Well it's not that hard seriously, did it with great success in the past, but of course something can go wrong for tons of reasons. Someone ever designed and cloned a synthetic organism? Way harder than growing shrooms i'd say! But if someone tries to do that and asks me something i just try to help, damn!

I don't think you can say i've lacked of humilty just cause i said i'm a biologist :confused: everybody sometimes needs advices from more experienced people and that's all i asked.

Really sad to find this kind of people here, you don't really want to help, just demostrate you are better growers! well i'll never ask again :wink: i'll just post my results next time

p.s. I asked my brother and found out my cousin slept in my room while I was out. Guess what, he opened all the jars to understand what was inside :facepalm3:  :facepalm3: just for u to know....


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Invisibleblindingleaf
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682362 - 09/27/16 07:24 AM (7 years, 4 months ago)

Quote:

i'll just post my results next time




thats all we ever wanted. we're simple folk


--------------------
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MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Invisiblefilthyknees
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682364 - 09/27/16 07:24 AM (7 years, 4 months ago)

Please do post your results


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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InvisibleInocuole
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Re: Rye jars driving a biologist crazy [Re: filthyknees]
    #23682371 - 09/27/16 07:27 AM (7 years, 4 months ago)

I wouldn't advise growing in a house with morons who will open your grain spawn to "understand what's inside".  That's pretty juvenile and reckless..  If it was in fact this then I don't understand why you're so fussy, that's not our faults, and it doesn't mean that the other things mentioned weren't potential vectors before that happened.


--------------------
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InvisibleAlmostBlue
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682397 - 09/27/16 07:41 AM (7 years, 4 months ago)

Quote:

Inocuole said:
I wouldn't advise growing in a house with morons who will open your grain spawn to "understand what's inside".  That's pretty juvenile and reckless..  If it was in fact this then I don't understand why you're so fussy, that's not our faults, and it doesn't mean that the other things mentioned weren't potential vectors before that happened.




Ever lived in a shared house? Ever had a junkie cousin? Is this juvenile and reckless?

By the way, my sterile technique was perfect I have to say, not the first time working sterile as i've isolated lots of weird bacteria and grown interesting biomaterials like bacterial cellulose and other stuff just for fun in the past. Plus i've years of lab experience.

But about dirty syringes, i want to understand this: is it really true? why do they sell contaminated syringes? it's not productive for a company to sell such a tricky product, and it's not hard to have a sterile procedure and a quality control system


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Invisibleblindingleaf
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682404 - 09/27/16 07:45 AM (7 years, 4 months ago)

its true that many are slightly contaminated, some are VERY contaminated, and few are 100% clean.

most vendors are purchasing spores in bulk, and making the syringes themselves.

bulk spores come from bulk grows which happen in unfiltered rooms.

edit: I'm not saying you can't do syringe>grain.  I'm not saying vendors are dishonest.  but its good to keep any potential vector in mind for the best chance of continued success


Edited by blindingleaf (09/27/16 07:48 AM)


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OfflineRhizomorpheus
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682418 - 09/27/16 08:02 AM (7 years, 4 months ago)

Quote:

nuentoter said:
Lesson learned, toss the junk, take a sliver of your white mycelium from your best looking grain and isolate on agar. Then go back to some clean grains with new filters.





https://www.shroomery.org/forums/showflat.php/Number/19208976
Check this out and do some agar dude it will be all good. Thanks for making this tek Pasty you da man... wherever u are =)

https://www.shroomery.org/forums/showflat.php/Number/20111637
After that check this tek video out.... Simple and done...  Thank you as well Tiger for a wonderful video on swabbing and dropping it like it hot <3

OP here is a suggestion my friend next time you make a post come with the respect even if you feel offended it's just the internet... kill em with kindness. Also I'm sure a lot of the older vets and peeps who have been here for a minute are tired of answering the same old questions over and over. Especially when we have a wonderful search engine available. I know how it is to be a noob... I still am one even with prior experience before the joining of this fine community. If you can't find the answer... then post. I have found plenty of problems that I have had or just things that I had wondered about by hitting it up. Low and behold I'm not the first person to have the issue or ask the question. No matter how much background or knowledge you may have in any field of this thing we call life... we always have time and things to learn. So get up shake it off move forward and get growing old school.
Peace Love Light and Blessings
:sporedrop:


--------------------
=) All smiles, all equaL (= Anything I post to this forum came from a book. All of it is entirely based in fiction and is for amusement purposes only. The dude hopes you enjoy it as much as he has. It's all just a dreaM.
:pipesmoke:


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InvisibleInocuole
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Re: Rye jars driving a biologist crazy [Re: Rhizomorpheus]
    #23682436 - 09/27/16 08:10 AM (7 years, 4 months ago)

:whathesaid: except don't grow old school, because some of those methods are pretty bunk.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineTheLongComma
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682472 - 09/27/16 08:28 AM (7 years, 4 months ago)

This guy has to be trolling...


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Offlinenuentoter
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682487 - 09/27/16 08:32 AM (7 years, 4 months ago)

I don't think anyone in her was outright negative towards you. Simply reacting to the attitude put forth by yourself. You did have a slightly cocky demeanor about being a molecular biologist therefore knowing a lot.

I'll take someone with good method growing mushrooms in their bedroom for half a decade (experience) over a biologist with plenty of knowledge but little experience. Not saying lack, just stating.

The idea that you don't know all, is a hard pushed ideology here on shroomery, a little humbleness goes a long way here socially.

And yes from spore would be optimal in this situation but I was under the impression the LC was gone by now, along with syringe. If I'm wrong, start with syringe > agar > grain or LC.

Out of curiosity did you use sab?


--------------------

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Re: Rye jars driving a biologist crazy [Re: Inocuole]
    #23682489 - 09/27/16 08:33 AM (7 years, 4 months ago)

Quote:

Inocuole said:
:whathesaid: except don't grow old school, because some of those methods are pretty bunk.




I was saying with the actions and things that had been said. I figured he may have been in his late 30s or 40s. In my late 20s and hang out with a lot of old school cats lol. Please in creations name don't grow old school or from some book with a forward wrote 30 years ago by Terence Mckenna...something like that is way way ancient shit. These books have great stuff to look at but new methods and information have been unearthed from psychonauts from all over. Mad scientist testing things out in the lab and getting shit done. Find what works for you and put it to use always fallow the tek if your doing one otherwise your just experimenting. There is nothing wrong with experiments once your further down the line but for now you research growth patterns as well as how to take the best care of your babies. Also keep your sterilization techniques to a T and produce bountiful fruits of labor. All this goes down while u flood your brain with information from the site to make the next step in your adventure. You have to crawl before you walk and that takes time but everyone has faith in you OP.


Edited by Rhizomorpheus (09/27/16 08:35 AM)


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Re: Rye jars driving a biologist crazy [Re: nuentoter]
    #23682515 - 09/27/16 08:45 AM (7 years, 4 months ago)

Quote:

nuentoter said:
I don't think anyone in her was outright negative towards you. Simply reacting to the attitude put forth by yourself. You did have a slightly cocky demeanor about being a molecular biologist therefore knowing a lot.

I'll take someone with good method growing mushrooms in their bedroom for half a decade (experience) over a biologist with plenty of knowledge but little experience. Not saying lack, just stating.

The idea that you don't know all, is a hard pushed ideology here on shroomery, a little humbleness goes a long way here socially.

And yes from spore would be optimal in this situation but I was under the impression the LC was gone by now, along with syringe. If I'm wrong, start with syringe > agar > grain or LC.

Out of curiosity did you use sab?





That's so funny man! I've just said that i have a biology background BUT i came here asking for help due to my lack of experience in this field, and i asked a specific question.

I'm totally ok with it, i said it myself, the fact is that people started making fun of me instead of helping, like: hey you are a biologist but your jar is shit, hey what are they teaching u in school, hey you don't know shit about growing mushrooms.

So is this shroomery ideals? hahahaha nice place to hang out


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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682527 - 09/27/16 08:51 AM (7 years, 4 months ago)

u also came here with a head full of self-congratulations man.

I'm just busting your balls.  have a sense of humor.  I'm on the tame side, read around, there are way worse things I could have said to you bud:lol:


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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682538 - 09/27/16 08:55 AM (7 years, 4 months ago)

It's only harsh if you take it personally. Being here for years, some of these questions like in your thread, have been asked a hundred times so some people get snappy. Like I said, is only a problem if you take it personally.

I understand, you're trying to get better experience through smarter choices and practices shared on this website. There is a plethora of info here if you dig. Ya dig?

Start over and take your time, put more thought into the weak points (where you put your jars) and you should be golden. For a long time, once things were in jars I put them in the pantry on top shelf in back, inconspicuous and plausible defence of "those are just nasty old canned veggies".


--------------------

The geometry of us is no chance. We are antennae, we are tuning forks, we are receiver and transmitters of all energy. We are more than we know.  - @entheolove

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Re: Rye jars driving a biologist crazy [Re: nuentoter]
    #23682561 - 09/27/16 09:04 AM (7 years, 4 months ago)



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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
    #23682567 - 09/27/16 09:06 AM (7 years, 4 months ago)

Quote:

AlmostBlue said:
Quote:

nuentoter said:
I don't think anyone in her was outright negative towards you. Simply reacting to the attitude put forth by yourself. You did have a slightly cocky demeanor about being a molecular biologist therefore knowing a lot.

I'll take someone with good method growing mushrooms in their bedroom for half a decade (experience) over a biologist with plenty of knowledge but little experience. Not saying lack, just stating.

The idea that you don't know all, is a hard pushed ideology here on shroomery, a little humbleness goes a long way here socially.

And yes from spore would be optimal in this situation but I was under the impression the LC was gone by now, along with syringe. If I'm wrong, start with syringe > agar > grain or LC.

Out of curiosity did you use sab?





That's so funny man! I've just said that i have a biology background BUT i came here asking for help due to my lack of experience in this field, and i asked a specific question.

I'm totally ok with it, i said it myself, the fact is that people started making fun of me instead of helping, like: hey you are a biologist but your jar is shit, hey what are they teaching u in school, hey you don't know shit about growing mushrooms.

So is this shroomery ideals? hahahaha nice place to hang out




The shroomery is an awesome place to chill out away from the bullshit of society. Sure you still deal with people's opinions and drops of the should and shouldn't. You will also have people voicing personal problems etc all kinds of stuff almost infinite. But dude this is the Internet.... complete freedom in some sense. People aren't going to put a filter on just because it makes you comfortable.... it's their right given to them by whatever created the reality we are plugged into. Get over it swallow the pride and move forward no point in getting hung up on words. I keep saying this.... so much is lost in language. Just kill it already and get growing your fueling a fire that just needs to burn out. You have no point to be made in burning the whole forest down brother.
:holyshit:
Quote:

mrmazdarx9 said:





:rofl:


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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682574 - 09/27/16 09:09 AM (7 years, 4 months ago)

Nobody made fun of you.
This is the internet, grow a thicker skin.
Your lack of self awareness is astounding.

Listen to the people who have grown more cubes than you can imagine.

Let me lay out all the incorrect things you did that people already corrected you on and you completely ignored. I'll try to be objective:

Your jars are contaminated, they were likely this way from the start.
People show up on the boards daily with the same exact problem because they went straight from spore syringe to grains or LC.
Spores are always going to contain some amount of bacteria and even mold spores.
This is because the mushrooms these spores come from are grown in open air.

Bacteria will outpace cubensis mycelium nearly always, even moreso in LC.
You can't tell a clean LC just by looking at it, everyone here will tell you this. I say this from experience.

Toss the jars and LC, you have no one to blame but yourself.

Start over and do the necessary research first.
Start with agar, we use it to get a clean culture free of obvious bacteria.
You may have grown oysters in the past but they're quite a bit different and more vigorous than cubes.

Lose the cocky attitude assuming you even stick around.

You also clearly have not read the rules, you listed a spore vendor name in the context of growing/mushcult which is not allowed.


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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682669 - 09/27/16 09:49 AM (7 years, 4 months ago)

Quote:

AlmostBlue said:

My major experience is in molecular biology, things you can't really do at home and probably the most of you don't understand but i'm not bitching around cause i have genetic engineering skills, which i'm more likely to share with a open community than use to make fun of others!







genetic engineering eh? 

https://www.shroomery.org/forums/showflat.php/Number/23669528#23669528
https://www.shroomery.org/forums/showflat.php/Number/23675033#23675033


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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
    #23682689 - 09/27/16 09:58 AM (7 years, 4 months ago)

Quote:

blindingleaf said:
u also came here with a head full of self-congratulations man.

I'm just busting your balls.  have a sense of humor.  I'm on the tame side, read around, there are way worse things I could have said to you bud:lol:




Shit leaf you are one of the nicest people round these parts hands down. Even when your a dick it's like yer pumping peeps tires lol.

This thread

:canthelpbutlaugh:


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Re: Rye jars driving a biologist crazy [Re: ballsalsa]
    #23682702 - 09/27/16 10:02 AM (7 years, 4 months ago)

Syringes are often unreliable. .use a sab not a counter....don't inoculate through the poly crack the lids...spores to lc is a shitty way to go...you rely on the syringe being clean if it's clean just use it and g2g ....one last noten. ..quit acting up and welcome to shroomery


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Re: Rye jars driving a biologist crazy [Re: cronicr]
    #23682707 - 09/27/16 10:03 AM (7 years, 4 months ago)

heh, even mods have reply to: fails


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Re: Rye jars driving a biologist crazy [Re: Pastywhyte]
    #23682710 - 09/27/16 10:03 AM (7 years, 4 months ago)

Quote:

Pastywhyte said:
Quote:

blindingleaf said:
u also came here with a head full of self-congratulations man.

I'm just busting your balls.  have a sense of humor.  I'm on the tame side, read around, there are way worse things I could have said to you bud:lol:




Shit leaf you are one of the nicest people round these parts hands down. Even when your a dick it's like yer pumping peeps tires lol.

This thread

:canthelpbutlaugh:




Hey Pasty!!  Thanks for helping me get into agar by creating your tek. Really gave me the push into something that intimidated me when I started my studies. Made it fun and kept me interested so I did not give up on such an awesome fucking hobby. Just thought I would throw u props.


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Re: Rye jars driving a biologist crazy [Re: ballsalsa]
    #23682716 - 09/27/16 10:06 AM (7 years, 4 months ago)

Quote:

ballsalsa said:
heh, even mods have reply to: fails



I wasn't about to read a whole second page of that shit lol...nice to see ya balls lol


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Re: Rye jars driving a biologist crazy [Re: Pastywhyte]
    #23682719 - 09/27/16 10:06 AM (7 years, 4 months ago)

Yeah seriously it's like coming in, showing your spaceship fruiting chamber, and bragging how you're an engineer. Doesn't matter how good you are at whatever field. You're in mycology territory now, and it's vastly different from any other field. Even botany.

But yeah any biologist realistically should know to start off any culture on agar. Including yeast, molds, bacteria, etc.


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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682793 - 09/27/16 10:29 AM (7 years, 4 months ago)

Quote:

AlmostBlue said:
Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great? :crazy2:

Now everybody saying that internet spores are junk, while lots of guides from trusted cultivators make use of spore syringes not mentioning this problem.

This forum's a real bitch!



Did you plate your syringe or LC on plate count?
You can't visually tell if a LC was clean. Even with a 150$ microscope you could have seen it was teeming with bacteria.


If you know how a syringe is made you know a syringe isn't 100% contaminant free. Injection lf a single CFU into a LC means that it will grow through the whole aqueous media and then you spread the fuck extremely easily

https://www.shroomery.org/forums/showflat.php/Number/21365919/fpart/1/vc/1

For 3000-5000$ anyone can make GMO yeast that expresses exogenous glucoamalayse from a bacterial gene in their own home...

Real world experience always beats still being in school for your bachelors degree


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Re: Rye jars driving a biologist crazy [Re: AlmostBlue] * 1
    #23682816 - 09/27/16 10:36 AM (7 years, 4 months ago)

Quote:



mornin everybody.

First post in this forum, but I've read all main threads by trusted cultivators and all the noob shit, even if i'm not a noob in mushroom cultivation.

Used to grow Oyster mycelium in various shapes, using solid supports or digitally fabricated cardboard puzzles, for design, bio-art, furniture crafting purposes.

Back in the days I was doing malt agar --> wood chips, straw, coffee grounds all sterilized in PC. Had no major contam problems.

Well now I wanted to challenge a friend of mine, all proud with his cambodian mycelium kit, wanted to show him how to do a proper grow as i'm a biologist and know lots about mushrooms and microbiology in general.

So 20 days ago I inoculated about 3 quarts of PC'd organic rye berries with multispore syringe (jars with one small hole in the lid, stuffed with extratight polifill, inoculated with sterile needle then put microtape over the lid, on a sanitized table and everything washed with disinfectant gels etc..) plus i tried different things for fun, a compact rice (grain) cake which is going pretty good, and a liquid culture which is also healthy.

Let's say 5 days ago the jars were all fully colonized, but had to wait for spawning to bulk because i was out of town until yesterday.

Well yesterday i shaked the fluffy white jars to check if they would recover in the night, woke up this morning and found out they are fucked up with green mold. Well this is insane, cause they were super healthy and fully colonised, how could this happen? isn't cubensis able to fight minor contams (popped up from nowhere, really) when fully colonized?

here some pics:







i have more pics if you ask.

In my opinion someone of my family found the jars, got curious and opened them, i can see no other explaination for these contams!

I know i could try to re-do the whole process with my liquid culture, but I don't have the time anymore, lots of shit to do.

Here's my question: the un-shaked jars still look nice and healty even if they are probably fucked up too. Is it worth to try a roll in vermiculite and birth as cakes, or a casing with verm/coir and put into fruiting conditions?

Thank you if you've read until here, thank you if you're gonna reply.






Just because this idiot deleted his post

And in case he deletes more

Quote:

AlmostBlue said:
Hey nerdy boy keep calm. Looks like you're not the only mushroom grower out there.

I say this because i'm pretty sure my filter system didn't fail as it was STERILE and SUPER TIGHT.

Also my siringe was ordered from the internet, and flame sterilized the needle every time in and out.

I've been out of town for 4 days and when I left it looked super healty that's why i think someone opened it while i was out.

Still you are a 'trusted cultivator' but didn't reply to my simple questions.

If you are on your period just leave my thread, thanks



Quote:

AlmostBlue said:
Quote:

Inocuole said:
If you wanted to show up your friend with a kit, inoculating grain with a a spore syringe was not the way to go about it.




yeah well i wanted to spawn the grain and colonize a big monotub, what's the right way to go about it?

ONLY RELEVANT REPLIES PLEASE, OTHERWISE JUST GO ON PORNHUB YOU'LL FEEL MORE CONFIDENT THEN



Quote:

AlmostBlue said:
Well, I felt like doing agar was not necessary as the syringe provider (inner visions) said the spore syringe was contam free, and i had no interest in selecting genetics, and little time to do the whole thing.

Opening a sterile jar to insert an agar slice isn't safer than inoculating from a hole if you assume your syringes are contam-free!

Never took out the polyfill, taped the outside after inoculation just to be safer.

Your sarcasm is totally pointless and just shows me how twisted and sick you guys are. Just give your opinion, not stupid pointless critiques just because you feel 'experts'.

I previously realized a 3d carboard puzzle model of an ancient building, sterilized, filled it with substrate and spawn, let it colonize and managed to fruit beautiful mushrooms from the 'windows' of the building. and i was 18yo at the time.

As I said, you are not the only mushroom growers out there. It's pretty easy actually! So stop acting like pussies and tell me if it's worth fruiting the unshaken jars with a casing layer or just as cakes rolled in verm, which was the ONLY question i've asked so far.



Quote:

AlmostBlue said:
Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great? :crazy2:

Now everybody saying that internet spores are junk, while lots of guides from trusted cultivators make use of spore syringes not mentioning this problem.

This forum's a real bitch!



Quote:

AlmostBlue said:
Of course i have some ideas (...) actually i'm asking what to do cause here i can find people with more experience with these techniques and if some real expert says i'm just gonna lose my time i'd toss everything away and go fuck myself.

My major experience is in molecular biology, things you can't really do at home and probably the most of you don't understand but i'm not bitching around cause i have genetic engineering skills, which i'm more likely to share with a open community than use to make fun of others!

I'll try anyway, some cased with coir and some rolled in verm and set in fruiting chamber

What a bunch of psychos i was just asking a REALLY SIMPLE question, and still the most of you just wrote something else to make fun of me (how poor you are), not really reading my first post about trying to save unshaken jars rather than starting the whole process with new grains due to no time. Read-->Understand-->Reply is a hard protocol nowdays





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Re: Rye jars driving a biologist crazy [Re: bodhisatta]
    #23682890 - 09/27/16 11:08 AM (7 years, 4 months ago)

Damn  I thought I smelled trees burning:facepalm3:
Thanks for keeping his epic fail online forever.

It must be the time limit....


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Edited by Rhizomorpheus (09/27/16 11:10 AM)


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Re: Rye jars driving a biologist crazy [Re: Rhizomorpheus]
    #23683121 - 09/27/16 12:42 PM (7 years, 4 months ago)

Haha nice one bod:highfive:


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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
    #23683235 - 09/27/16 01:37 PM (7 years, 4 months ago)

damn i passed out and missed this shit post. lame.

but ill contribute this!!!!

id think a biologist would also use better grammar and not use
'u' for you.

and also have a grasp on the 'scientific method'
https://en.wikipedia.org/wiki/Scientific_method

rather than blame the 'junkie cousin' who ruined your 'super clean grow'
you sound like donald trump.

ahhh that felt good.


Edited by mushboy (09/27/16 01:48 PM)


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Re: Rye jars driving a biologist crazy [Re: mushboy] * 1
    #23683241 - 09/27/16 01:38 PM (7 years, 4 months ago)

Banned..aww that felt good to now let's knock the shit off.


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Re: Rye jars driving a biologist crazy [Re: cronicr]
    #23684197 - 09/27/16 06:56 PM (7 years, 4 months ago)

You know full well this thread can never be repurposed for anything useful.


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Re: Rye jars driving a biologist crazy [Re: Inocuole] * 1
    #23684258 - 09/27/16 07:18 PM (7 years, 4 months ago)

We should create a collection of threads made by biologists, mycologists, engineers, PhD's, and people who have been doing this for a very long time. This thread could be a link in the sea of threads.


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Re: Rye jars driving a biologist crazy [Re: Pastywhyte]
    #23684265 - 09/27/16 07:19 PM (7 years, 4 months ago)

That's a pretty good and hilarious idea, actually.  Would love to see every "I'm an engineer/biologist" etc.  post in one place.  Maybe then the point would start to hit home.


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