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OfflinecronicrFacebook
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Re: Rye jars driving a biologist crazy [Re: ballsalsa]
    #23682702 - 09/27/16 10:02 AM (7 years, 4 months ago)

Syringes are often unreliable. .use a sab not a counter....don't inoculate through the poly crack the lids...spores to lc is a shitty way to go...you rely on the syringe being clean if it's clean just use it and g2g ....one last noten. ..quit acting up and welcome to shroomery


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InvisibleballsalsaMDiscord
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Re: Rye jars driving a biologist crazy [Re: cronicr]
    #23682707 - 09/27/16 10:03 AM (7 years, 4 months ago)

heh, even mods have reply to: fails


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OfflineRhizomorpheus
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Re: Rye jars driving a biologist crazy [Re: Pastywhyte]
    #23682710 - 09/27/16 10:03 AM (7 years, 4 months ago)

Quote:

Pastywhyte said:
Quote:

blindingleaf said:
u also came here with a head full of self-congratulations man.

I'm just busting your balls.  have a sense of humor.  I'm on the tame side, read around, there are way worse things I could have said to you bud:lol:




Shit leaf you are one of the nicest people round these parts hands down. Even when your a dick it's like yer pumping peeps tires lol.

This thread

:canthelpbutlaugh:




Hey Pasty!!  Thanks for helping me get into agar by creating your tek. Really gave me the push into something that intimidated me when I started my studies. Made it fun and kept me interested so I did not give up on such an awesome fucking hobby. Just thought I would throw u props.


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OfflinecronicrFacebook
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Re: Rye jars driving a biologist crazy [Re: ballsalsa]
    #23682716 - 09/27/16 10:06 AM (7 years, 4 months ago)

Quote:

ballsalsa said:
heh, even mods have reply to: fails



I wasn't about to read a whole second page of that shit lol...nice to see ya balls lol


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InvisibleMad Season
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Re: Rye jars driving a biologist crazy [Re: Pastywhyte]
    #23682719 - 09/27/16 10:06 AM (7 years, 4 months ago)

Yeah seriously it's like coming in, showing your spaceship fruiting chamber, and bragging how you're an engineer. Doesn't matter how good you are at whatever field. You're in mycology territory now, and it's vastly different from any other field. Even botany.

But yeah any biologist realistically should know to start off any culture on agar. Including yeast, molds, bacteria, etc.


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InvisiblebodhisattaMDiscordReddit
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
    #23682793 - 09/27/16 10:29 AM (7 years, 4 months ago)

Quote:

AlmostBlue said:
Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great? :crazy2:

Now everybody saying that internet spores are junk, while lots of guides from trusted cultivators make use of spore syringes not mentioning this problem.

This forum's a real bitch!



Did you plate your syringe or LC on plate count?
You can't visually tell if a LC was clean. Even with a 150$ microscope you could have seen it was teeming with bacteria.


If you know how a syringe is made you know a syringe isn't 100% contaminant free. Injection lf a single CFU into a LC means that it will grow through the whole aqueous media and then you spread the fuck extremely easily

https://www.shroomery.org/forums/showflat.php/Number/21365919/fpart/1/vc/1

For 3000-5000$ anyone can make GMO yeast that expresses exogenous glucoamalayse from a bacterial gene in their own home...

Real world experience always beats still being in school for your bachelors degree


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InvisiblebodhisattaMDiscordReddit
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue] * 1
    #23682816 - 09/27/16 10:36 AM (7 years, 4 months ago)

Quote:



mornin everybody.

First post in this forum, but I've read all main threads by trusted cultivators and all the noob shit, even if i'm not a noob in mushroom cultivation.

Used to grow Oyster mycelium in various shapes, using solid supports or digitally fabricated cardboard puzzles, for design, bio-art, furniture crafting purposes.

Back in the days I was doing malt agar --> wood chips, straw, coffee grounds all sterilized in PC. Had no major contam problems.

Well now I wanted to challenge a friend of mine, all proud with his cambodian mycelium kit, wanted to show him how to do a proper grow as i'm a biologist and know lots about mushrooms and microbiology in general.

So 20 days ago I inoculated about 3 quarts of PC'd organic rye berries with multispore syringe (jars with one small hole in the lid, stuffed with extratight polifill, inoculated with sterile needle then put microtape over the lid, on a sanitized table and everything washed with disinfectant gels etc..) plus i tried different things for fun, a compact rice (grain) cake which is going pretty good, and a liquid culture which is also healthy.

Let's say 5 days ago the jars were all fully colonized, but had to wait for spawning to bulk because i was out of town until yesterday.

Well yesterday i shaked the fluffy white jars to check if they would recover in the night, woke up this morning and found out they are fucked up with green mold. Well this is insane, cause they were super healthy and fully colonised, how could this happen? isn't cubensis able to fight minor contams (popped up from nowhere, really) when fully colonized?

here some pics:







i have more pics if you ask.

In my opinion someone of my family found the jars, got curious and opened them, i can see no other explaination for these contams!

I know i could try to re-do the whole process with my liquid culture, but I don't have the time anymore, lots of shit to do.

Here's my question: the un-shaked jars still look nice and healty even if they are probably fucked up too. Is it worth to try a roll in vermiculite and birth as cakes, or a casing with verm/coir and put into fruiting conditions?

Thank you if you've read until here, thank you if you're gonna reply.






Just because this idiot deleted his post

And in case he deletes more

Quote:

AlmostBlue said:
Hey nerdy boy keep calm. Looks like you're not the only mushroom grower out there.

I say this because i'm pretty sure my filter system didn't fail as it was STERILE and SUPER TIGHT.

Also my siringe was ordered from the internet, and flame sterilized the needle every time in and out.

I've been out of town for 4 days and when I left it looked super healty that's why i think someone opened it while i was out.

Still you are a 'trusted cultivator' but didn't reply to my simple questions.

If you are on your period just leave my thread, thanks



Quote:

AlmostBlue said:
Quote:

Inocuole said:
If you wanted to show up your friend with a kit, inoculating grain with a a spore syringe was not the way to go about it.




yeah well i wanted to spawn the grain and colonize a big monotub, what's the right way to go about it?

ONLY RELEVANT REPLIES PLEASE, OTHERWISE JUST GO ON PORNHUB YOU'LL FEEL MORE CONFIDENT THEN



Quote:

AlmostBlue said:
Well, I felt like doing agar was not necessary as the syringe provider (inner visions) said the spore syringe was contam free, and i had no interest in selecting genetics, and little time to do the whole thing.

Opening a sterile jar to insert an agar slice isn't safer than inoculating from a hole if you assume your syringes are contam-free!

Never took out the polyfill, taped the outside after inoculation just to be safer.

Your sarcasm is totally pointless and just shows me how twisted and sick you guys are. Just give your opinion, not stupid pointless critiques just because you feel 'experts'.

I previously realized a 3d carboard puzzle model of an ancient building, sterilized, filled it with substrate and spawn, let it colonize and managed to fruit beautiful mushrooms from the 'windows' of the building. and i was 18yo at the time.

As I said, you are not the only mushroom growers out there. It's pretty easy actually! So stop acting like pussies and tell me if it's worth fruiting the unshaken jars with a casing layer or just as cakes rolled in verm, which was the ONLY question i've asked so far.



Quote:

AlmostBlue said:
Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great? :crazy2:

Now everybody saying that internet spores are junk, while lots of guides from trusted cultivators make use of spore syringes not mentioning this problem.

This forum's a real bitch!



Quote:

AlmostBlue said:
Of course i have some ideas (...) actually i'm asking what to do cause here i can find people with more experience with these techniques and if some real expert says i'm just gonna lose my time i'd toss everything away and go fuck myself.

My major experience is in molecular biology, things you can't really do at home and probably the most of you don't understand but i'm not bitching around cause i have genetic engineering skills, which i'm more likely to share with a open community than use to make fun of others!

I'll try anyway, some cased with coir and some rolled in verm and set in fruiting chamber

What a bunch of psychos i was just asking a REALLY SIMPLE question, and still the most of you just wrote something else to make fun of me (how poor you are), not really reading my first post about trying to save unshaken jars rather than starting the whole process with new grains due to no time. Read-->Understand-->Reply is a hard protocol nowdays





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OfflineRhizomorpheus
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Re: Rye jars driving a biologist crazy [Re: bodhisatta]
    #23682890 - 09/27/16 11:08 AM (7 years, 4 months ago)

Damn  I thought I smelled trees burning:facepalm3:
Thanks for keeping his epic fail online forever.

It must be the time limit....


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=) All smiles, all equaL (= Anything I post to this forum came from a book. All of it is entirely based in fiction and is for amusement purposes only. The dude hopes you enjoy it as much as he has. It's all just a dreaM.
:pipesmoke:


Edited by Rhizomorpheus (09/27/16 11:10 AM)


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Offlinemrmazdarx9
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Re: Rye jars driving a biologist crazy [Re: Rhizomorpheus]
    #23683121 - 09/27/16 12:42 PM (7 years, 4 months ago)

Haha nice one bod:highfive:


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InvisiblemushboyMDiscord
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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
    #23683235 - 09/27/16 01:37 PM (7 years, 4 months ago)

damn i passed out and missed this shit post. lame.

but ill contribute this!!!!

id think a biologist would also use better grammar and not use
'u' for you.

and also have a grasp on the 'scientific method'
https://en.wikipedia.org/wiki/Scientific_method

rather than blame the 'junkie cousin' who ruined your 'super clean grow'
you sound like donald trump.

ahhh that felt good.


Edited by mushboy (09/27/16 01:48 PM)


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OfflinecronicrFacebook
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Re: Rye jars driving a biologist crazy [Re: mushboy] * 1
    #23683241 - 09/27/16 01:38 PM (7 years, 4 months ago)

Banned..aww that felt good to now let's knock the shit off.


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InvisibleInocuole
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Re: Rye jars driving a biologist crazy [Re: cronicr]
    #23684197 - 09/27/16 06:56 PM (7 years, 4 months ago)

You know full well this thread can never be repurposed for anything useful.


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Re: Rye jars driving a biologist crazy [Re: Inocuole] * 1
    #23684258 - 09/27/16 07:18 PM (7 years, 4 months ago)

We should create a collection of threads made by biologists, mycologists, engineers, PhD's, and people who have been doing this for a very long time. This thread could be a link in the sea of threads.


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Re: Rye jars driving a biologist crazy [Re: Pastywhyte]
    #23684265 - 09/27/16 07:19 PM (7 years, 4 months ago)

That's a pretty good and hilarious idea, actually.  Would love to see every "I'm an engineer/biologist" etc.  post in one place.  Maybe then the point would start to hit home.


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