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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682315 - 09/27/16 06:56 AM (7 years, 4 months ago) |
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a little humility and a sense of humor go a long way.
ur post could have just been the pictures and a question. that would have been a "really simple" way to get a "read>understand>reply" response.
don't take us as seriously as u take urself dude.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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AlmostBlue
Stranger

Registered: 09/20/16
Posts: 9
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682354 - 09/27/16 07:22 AM (7 years, 4 months ago) |
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Quote:
Inocuole said:
Quote:
nuentoter said: Lesson learned, toss the junk, take a sliver of your white mycelium from your best looking grain and isolate on agar. Then go back to some clean grains with new filters.
I wouldn't bother trying to save that mycelium in particular when he could just start from spores again and maybe have a better chance of isolating something clean straight away. If mold gets a chance to interlace with a culture, then it's difficult/near impossible to separate them. Bacteria's a little easier but still a pain.
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AlmostBlue said: Then another one for you experts, if the syringe was dirty, how do you explain the rice cake and the liquid culture doing great? 
Made LC with spores, thinks it's doing great. 
I figured you could take it bluntly since you were a biologist but I guess you're one of those experts... the ones that already know everything and dismiss good advice while flaunting their mistakes as if they're marks of success. I didn't anticipate you getting your feelings hurt though. Quite unfortunate.
If you want to know why people were short with you to begin with, it's because you said this
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Well now I wanted to challenge a friend of mine, all proud with his cambodian mycelium kit, wanted to show him how to do a proper grow as i'm a biologist and know lots about mushrooms and microbiology in general.
And then proceeded to list off one noob thing after another, like you may as well have never heard of mushrooms at all prior to this. A proper grow goes a lot smoother with a proper mindset, and I don't think anybody here is seeing that you have that..
lol what's weird about an LC from spores? Using mycelium tissue is of course faster but do you know spores can properly germinate and have sex in a LC?
But all this is related to the fact I said I'm a (molecular)biologist so you guys growing mushrooms at your place since who knows when felt like you had to give me a lesson. Well there's no lesson to give my friends, i just asked 2 very simple questions in the first post. Almost none of you replied with something useful, just bitching about how experienced in growing mushrooms you are. Well it's not that hard seriously, did it with great success in the past, but of course something can go wrong for tons of reasons. Someone ever designed and cloned a synthetic organism? Way harder than growing shrooms i'd say! But if someone tries to do that and asks me something i just try to help, damn!
I don't think you can say i've lacked of humilty just cause i said i'm a biologist everybody sometimes needs advices from more experienced people and that's all i asked.
Really sad to find this kind of people here, you don't really want to help, just demostrate you are better growers! well i'll never ask again i'll just post my results next time
p.s. I asked my brother and found out my cousin slept in my room while I was out. Guess what, he opened all the jars to understand what was inside just for u to know....
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682362 - 09/27/16 07:24 AM (7 years, 4 months ago) |
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Quote:
i'll just post my results next time
thats all we ever wanted. we're simple folk
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682364 - 09/27/16 07:24 AM (7 years, 4 months ago) |
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Please do post your results
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Rye jars driving a biologist crazy [Re: filthyknees]
#23682371 - 09/27/16 07:27 AM (7 years, 4 months ago) |
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I wouldn't advise growing in a house with morons who will open your grain spawn to "understand what's inside". That's pretty juvenile and reckless.. If it was in fact this then I don't understand why you're so fussy, that's not our faults, and it doesn't mean that the other things mentioned weren't potential vectors before that happened.
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AlmostBlue
Stranger

Registered: 09/20/16
Posts: 9
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682397 - 09/27/16 07:41 AM (7 years, 4 months ago) |
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Quote:
Inocuole said: I wouldn't advise growing in a house with morons who will open your grain spawn to "understand what's inside". That's pretty juvenile and reckless.. If it was in fact this then I don't understand why you're so fussy, that's not our faults, and it doesn't mean that the other things mentioned weren't potential vectors before that happened.
Ever lived in a shared house? Ever had a junkie cousin? Is this juvenile and reckless?
By the way, my sterile technique was perfect I have to say, not the first time working sterile as i've isolated lots of weird bacteria and grown interesting biomaterials like bacterial cellulose and other stuff just for fun in the past. Plus i've years of lab experience.
But about dirty syringes, i want to understand this: is it really true? why do they sell contaminated syringes? it's not productive for a company to sell such a tricky product, and it's not hard to have a sterile procedure and a quality control system
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682404 - 09/27/16 07:45 AM (7 years, 4 months ago) |
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its true that many are slightly contaminated, some are VERY contaminated, and few are 100% clean.
most vendors are purchasing spores in bulk, and making the syringes themselves.
bulk spores come from bulk grows which happen in unfiltered rooms.
edit: I'm not saying you can't do syringe>grain. I'm not saying vendors are dishonest. but its good to keep any potential vector in mind for the best chance of continued success
Edited by blindingleaf (09/27/16 07:48 AM)
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Rhizomorpheus
PrototypE


Registered: 08/07/16
Posts: 135
Loc: Deep SpacE
Last seen: 4 years, 2 months
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682418 - 09/27/16 08:02 AM (7 years, 4 months ago) |
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Quote:
nuentoter said: Lesson learned, toss the junk, take a sliver of your white mycelium from your best looking grain and isolate on agar. Then go back to some clean grains with new filters.
https://www.shroomery.org/forums/showflat.php/Number/19208976 Check this out and do some agar dude it will be all good. Thanks for making this tek Pasty you da man... wherever u are =)
https://www.shroomery.org/forums/showflat.php/Number/20111637 After that check this tek video out.... Simple and done... Thank you as well Tiger for a wonderful video on swabbing and dropping it like it hot <3
OP here is a suggestion my friend next time you make a post come with the respect even if you feel offended it's just the internet... kill em with kindness. Also I'm sure a lot of the older vets and peeps who have been here for a minute are tired of answering the same old questions over and over. Especially when we have a wonderful search engine available. I know how it is to be a noob... I still am one even with prior experience before the joining of this fine community. If you can't find the answer... then post. I have found plenty of problems that I have had or just things that I had wondered about by hitting it up. Low and behold I'm not the first person to have the issue or ask the question. No matter how much background or knowledge you may have in any field of this thing we call life... we always have time and things to learn. So get up shake it off move forward and get growing old school. Peace Love Light and Blessings
-------------------- =) All smiles, all equaL (= Anything I post to this forum came from a book. All of it is entirely based in fiction and is for amusement purposes only. The dude hopes you enjoy it as much as he has. It's all just a dreaM.
 
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Rye jars driving a biologist crazy [Re: Rhizomorpheus]
#23682436 - 09/27/16 08:10 AM (7 years, 4 months ago) |
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except don't grow old school, because some of those methods are pretty bunk.
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TheLongComma
Sky Raisin


Registered: 08/07/16
Posts: 50
Last seen: 2 years, 9 months
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682472 - 09/27/16 08:28 AM (7 years, 4 months ago) |
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This guy has to be trolling...
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nuentoter
conduit



Registered: 09/17/08
Posts: 2,721
Last seen: 7 years, 21 days
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682487 - 09/27/16 08:32 AM (7 years, 4 months ago) |
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I don't think anyone in her was outright negative towards you. Simply reacting to the attitude put forth by yourself. You did have a slightly cocky demeanor about being a molecular biologist therefore knowing a lot.
I'll take someone with good method growing mushrooms in their bedroom for half a decade (experience) over a biologist with plenty of knowledge but little experience. Not saying lack, just stating.
The idea that you don't know all, is a hard pushed ideology here on shroomery, a little humbleness goes a long way here socially.
And yes from spore would be optimal in this situation but I was under the impression the LC was gone by now, along with syringe. If I'm wrong, start with syringe > agar > grain or LC.
Out of curiosity did you use sab?
--------------------
The geometry of us is no chance. We are antennae, we are tuning forks, we are receiver and transmitters of all energy. We are more than we know. - @entheolove "I found I could say things with color and shapes that I couldn't say any other way - things I had no words for" - Georgia O'Keefe I think the word is vagina
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Rhizomorpheus
PrototypE


Registered: 08/07/16
Posts: 135
Loc: Deep SpacE
Last seen: 4 years, 2 months
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Re: Rye jars driving a biologist crazy [Re: Inocuole]
#23682489 - 09/27/16 08:33 AM (7 years, 4 months ago) |
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Quote:
Inocuole said:
except don't grow old school, because some of those methods are pretty bunk.
I was saying with the actions and things that had been said. I figured he may have been in his late 30s or 40s. In my late 20s and hang out with a lot of old school cats lol. Please in creations name don't grow old school or from some book with a forward wrote 30 years ago by Terence Mckenna...something like that is way way ancient shit. These books have great stuff to look at but new methods and information have been unearthed from psychonauts from all over. Mad scientist testing things out in the lab and getting shit done. Find what works for you and put it to use always fallow the tek if your doing one otherwise your just experimenting. There is nothing wrong with experiments once your further down the line but for now you research growth patterns as well as how to take the best care of your babies. Also keep your sterilization techniques to a T and produce bountiful fruits of labor. All this goes down while u flood your brain with information from the site to make the next step in your adventure. You have to crawl before you walk and that takes time but everyone has faith in you OP.
Edited by Rhizomorpheus (09/27/16 08:35 AM)
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AlmostBlue
Stranger

Registered: 09/20/16
Posts: 9
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Re: Rye jars driving a biologist crazy [Re: nuentoter]
#23682515 - 09/27/16 08:45 AM (7 years, 4 months ago) |
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Quote:
nuentoter said: I don't think anyone in her was outright negative towards you. Simply reacting to the attitude put forth by yourself. You did have a slightly cocky demeanor about being a molecular biologist therefore knowing a lot.
I'll take someone with good method growing mushrooms in their bedroom for half a decade (experience) over a biologist with plenty of knowledge but little experience. Not saying lack, just stating.
The idea that you don't know all, is a hard pushed ideology here on shroomery, a little humbleness goes a long way here socially.
And yes from spore would be optimal in this situation but I was under the impression the LC was gone by now, along with syringe. If I'm wrong, start with syringe > agar > grain or LC.
Out of curiosity did you use sab?
That's so funny man! I've just said that i have a biology background BUT i came here asking for help due to my lack of experience in this field, and i asked a specific question.
I'm totally ok with it, i said it myself, the fact is that people started making fun of me instead of helping, like: hey you are a biologist but your jar is shit, hey what are they teaching u in school, hey you don't know shit about growing mushrooms.
So is this shroomery ideals? hahahaha nice place to hang out
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682527 - 09/27/16 08:51 AM (7 years, 4 months ago) |
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u also came here with a head full of self-congratulations man.
I'm just busting your balls. have a sense of humor. I'm on the tame side, read around, there are way worse things I could have said to you bud
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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nuentoter
conduit



Registered: 09/17/08
Posts: 2,721
Last seen: 7 years, 21 days
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682538 - 09/27/16 08:55 AM (7 years, 4 months ago) |
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It's only harsh if you take it personally. Being here for years, some of these questions like in your thread, have been asked a hundred times so some people get snappy. Like I said, is only a problem if you take it personally.
I understand, you're trying to get better experience through smarter choices and practices shared on this website. There is a plethora of info here if you dig. Ya dig?
Start over and take your time, put more thought into the weak points (where you put your jars) and you should be golden. For a long time, once things were in jars I put them in the pantry on top shelf in back, inconspicuous and plausible defence of "those are just nasty old canned veggies".
--------------------
The geometry of us is no chance. We are antennae, we are tuning forks, we are receiver and transmitters of all energy. We are more than we know. - @entheolove "I found I could say things with color and shapes that I couldn't say any other way - things I had no words for" - Georgia O'Keefe I think the word is vagina
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: Rye jars driving a biologist crazy [Re: nuentoter]
#23682561 - 09/27/16 09:04 AM (7 years, 4 months ago) |
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-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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Rhizomorpheus
PrototypE


Registered: 08/07/16
Posts: 135
Loc: Deep SpacE
Last seen: 4 years, 2 months
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Re: Rye jars driving a biologist crazy [Re: mrmazdarx9]
#23682567 - 09/27/16 09:06 AM (7 years, 4 months ago) |
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Quote:
AlmostBlue said:
Quote:
nuentoter said: I don't think anyone in her was outright negative towards you. Simply reacting to the attitude put forth by yourself. You did have a slightly cocky demeanor about being a molecular biologist therefore knowing a lot.
I'll take someone with good method growing mushrooms in their bedroom for half a decade (experience) over a biologist with plenty of knowledge but little experience. Not saying lack, just stating.
The idea that you don't know all, is a hard pushed ideology here on shroomery, a little humbleness goes a long way here socially.
And yes from spore would be optimal in this situation but I was under the impression the LC was gone by now, along with syringe. If I'm wrong, start with syringe > agar > grain or LC.
Out of curiosity did you use sab?
That's so funny man! I've just said that i have a biology background BUT i came here asking for help due to my lack of experience in this field, and i asked a specific question.
I'm totally ok with it, i said it myself, the fact is that people started making fun of me instead of helping, like: hey you are a biologist but your jar is shit, hey what are they teaching u in school, hey you don't know shit about growing mushrooms.
So is this shroomery ideals? hahahaha nice place to hang out
The shroomery is an awesome place to chill out away from the bullshit of society. Sure you still deal with people's opinions and drops of the should and shouldn't. You will also have people voicing personal problems etc all kinds of stuff almost infinite. But dude this is the Internet.... complete freedom in some sense. People aren't going to put a filter on just because it makes you comfortable.... it's their right given to them by whatever created the reality we are plugged into. Get over it swallow the pride and move forward no point in getting hung up on words. I keep saying this.... so much is lost in language. Just kill it already and get growing your fueling a fire that just needs to burn out. You have no point to be made in burning the whole forest down brother.

Quote:
mrmazdarx9 said:

-------------------- =) All smiles, all equaL (= Anything I post to this forum came from a book. All of it is entirely based in fiction and is for amusement purposes only. The dude hopes you enjoy it as much as he has. It's all just a dreaM.
 
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682574 - 09/27/16 09:09 AM (7 years, 4 months ago) |
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Nobody made fun of you. This is the internet, grow a thicker skin. Your lack of self awareness is astounding.
Listen to the people who have grown more cubes than you can imagine.
Let me lay out all the incorrect things you did that people already corrected you on and you completely ignored. I'll try to be objective:
Your jars are contaminated, they were likely this way from the start. People show up on the boards daily with the same exact problem because they went straight from spore syringe to grains or LC. Spores are always going to contain some amount of bacteria and even mold spores. This is because the mushrooms these spores come from are grown in open air.
Bacteria will outpace cubensis mycelium nearly always, even moreso in LC. You can't tell a clean LC just by looking at it, everyone here will tell you this. I say this from experience.
Toss the jars and LC, you have no one to blame but yourself.
Start over and do the necessary research first. Start with agar, we use it to get a clean culture free of obvious bacteria. You may have grown oysters in the past but they're quite a bit different and more vigorous than cubes.
Lose the cocky attitude assuming you even stick around.
You also clearly have not read the rules, you listed a spore vendor name in the context of growing/mushcult which is not allowed.
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ballsalsa
Universally Loathed and Reviled



Registered: 03/11/15
Posts: 20,876
Loc: Foreign Lands
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Re: Rye jars driving a biologist crazy [Re: AlmostBlue]
#23682669 - 09/27/16 09:49 AM (7 years, 4 months ago) |
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Quote:
AlmostBlue said:
My major experience is in molecular biology, things you can't really do at home and probably the most of you don't understand but i'm not bitching around cause i have genetic engineering skills, which i'm more likely to share with a open community than use to make fun of others!
genetic engineering eh?
https://www.shroomery.org/forums/showflat.php/Number/23669528#23669528 https://www.shroomery.org/forums/showflat.php/Number/23675033#23675033
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Like cannabis topics? Read my cannabis blog here
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Rye jars driving a biologist crazy [Re: blindingleaf]
#23682689 - 09/27/16 09:58 AM (7 years, 4 months ago) |
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Quote:
blindingleaf said: u also came here with a head full of self-congratulations man.
I'm just busting your balls. have a sense of humor. I'm on the tame side, read around, there are way worse things I could have said to you bud
Shit leaf you are one of the nicest people round these parts hands down. Even when your a dick it's like yer pumping peeps tires lol.
This thread
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