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OfflineSenorpengwin
Stranger
Registered: 09/14/16
Posts: 3
Last seen: 7 years, 4 months
Is this contam? What am I doing wrong?
    #23681416 - 09/26/16 09:41 PM (7 years, 4 months ago)

Hey guys, need some help, this is my first time (well second) but have not been successful in keep out mold.  First I just want to confirm from the pictures, it is starting to have a greenish tint, should I just toss these now?

Basically I have followed the PF Tek very closely, I used 1/2 pint jars with holes drilled in the top and covered with tape and foil.  I filled with BRF mixture and covered the top 1/2 with dry verm.  I used pressure cooker at 12psi for 100 minutes, left over night to cool. 
For inoculation I used a glove box and used bleach to clean it, inside and out.  I wiped everything down with rubbing alcohol that I would touch.  I used rubber gloves that go up to my elbow and also a face mask.  I quickly transferred the jars from pressure cooker to glove box.  Then I used torch on needle tip and lifted foil to puncture tape and inoculate.  Followed same procedure on second jar.  I transferred to a small box that I cleaned with bleach and put inside a cabinet. 


I tried this before with 5 jars and had the same result, what am I doing wrong?!?!  This is 3 days out.





Edited by Senorpengwin (09/27/16 05:36 PM)


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Offlinerider420
Ghost in the machine
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Registered: 02/11/16
Posts: 659
Last seen: 4 months, 7 days
Re: Is this contam? What am I doing wrong? [Re: Senorpengwin]
    #23681545 - 09/26/16 10:33 PM (7 years, 4 months ago)

Its in the air dude. Take a clear plastic bag that is garbage bag size. Fill it half full with air then add cakes needles and a spray bottle of alcohol. Tie air tight then spray everything with the alcohol using the coroners of the bag as gloves including the inside of the bag. Wait twenty minutes then inoculate the cakes using the corners of the bag as gloves. DO NOT FLAME inside the bag! Remove and place in warm area. Went from 20 to 60% contamination to zero. Best of luck.


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Is this contam? What am I doing wrong? [Re: rider420]
    #23682898 - 09/27/16 11:12 AM (7 years, 4 months ago)

:dafuq: not only was that incomprehensible, it sounded like garbage information. How could it be the air if every jar is getting it, and he's doing it in a glove box? This is obviously a syringe fault if every jar is getting it, and this is done in a GB. This is pf tek here. People did it in open air for the longest time..

OP you need to get clean inoculant. Test it out on agar and try to transfer clean growth, or complain a shit ton to the vendor saying all you see is contaminations under the scope, and get a new syringe..


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
Re: Is this contam? What am I doing wrong? [Re: Mad Season]
    #23683861 - 09/27/16 05:14 PM (7 years, 4 months ago)

Sounds like you did everything right, it must be the syringe then.


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OfflineSenorpengwin
Stranger
Registered: 09/14/16
Posts: 3
Last seen: 7 years, 4 months
Re: Is this contam? What am I doing wrong? [Re: Tira]
    #23683937 - 09/27/16 05:45 PM (7 years, 4 months ago)

:/ thanks for the info guys, at least it helps to pinpoint the weak point.

Could it have been something I did when I first got the syringe?  What about when I unscrewed the cap and actually screwed on the needle, could something have gotten in there?  or is it more likely a defective syringe?  The supplier I got it from had nothing but outstanding reviews.


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
Re: Is this contam? What am I doing wrong? [Re: Mad Season]
    #23683981 - 09/27/16 05:58 PM (7 years, 4 months ago)

I really don't think it was you, especially if you unscrewed the cap inside the glovebox.

It just happens, even the best supplier will get one or two contaminated syringes considering they make hundreds of them.

Quote:

Mad Season said:
complain a shit ton to the vendor saying all you see is contaminations under the scope, and get a new syringe..




I'd follow this path


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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