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InvisibleMycolorado
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Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA * 1
    #23681144 - 09/26/16 08:17 PM (7 years, 4 months ago)

I thought it would be interesting to look at the differences in growth rates and morphologies of p. cubensis mycelium on agar with different nutrient concentrations...2% & 4% MEA.  I'll post up pics as the plates progress.  Here are a couple taken today; already, the 2% is showing more of a rhizomorphic pattern.

2%

4%


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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23681178 - 09/26/16 08:25 PM (7 years, 4 months ago)

I've always read that the less nutrient makeup of the agar, the more rhizo the growth. Nice to see some backup for it :rockon:


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OfflineChristaphonZ
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Psilosoulful]
    #23681308 - 09/26/16 09:06 PM (7 years, 4 months ago)

Definitely watching this thread thank you for posting. Did you only try two plates?


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InvisibleMycolorado
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: ChristaphonZ]
    #23681522 - 09/26/16 10:23 PM (7 years, 4 months ago)

Yeah, it's not a great experiment as far as the method goes...needed a control, and more plates....however, I'm running the same comparison on a few edible and medicinal species but just sharing the cube progress here.  And yep, the lower nute agar should run faster and form more of a rhizo pattern where the 4% should take on more of a radially linear or tomentose form and run slower.  These are like a P7 transfer.


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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23681925 - 09/27/16 01:08 AM (7 years, 4 months ago)

Yays


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InvisibleMycolorado
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: tump]
    #23691784 - 09/29/16 07:11 PM (7 years, 3 months ago)

Here they are at day 5.  My basement hovers right around 70F so everything tends to move a bit slow down there.  So far, the results are the exact opposite of what I was expecting.  The mycelium on the 4% is clearly forming a much thicker set of ropey sectors.


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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23698167 - 10/01/16 07:24 PM (7 years, 3 months ago)

Wow, have the other plates continued this pattern? (The other species)


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InvisibleMycolorado
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: ChristaphonZ]
    #23698566 - 10/01/16 10:13 PM (7 years, 3 months ago)

Well, all the 4% plates are showing stronger growth with the exception of the king oyster which looks about the same as its 2% counterpart.  However the reishi is definitely showing the expected pattern (but also looks much stronger).  I'll prolly start using 4% as my standard with MEA.




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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23704570 - 10/03/16 06:18 PM (7 years, 3 months ago)



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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado] * 1
    #23714330 - 10/06/16 08:27 PM (7 years, 3 months ago)

Update here.  The 4% plate is showing an almost linear growth pattern at this point and the 2% is becoming rhizomorphic over a greater portion of the culture. 


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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23714377 - 10/06/16 08:44 PM (7 years, 3 months ago)

Very nice.
Really illustrates the difference well.

Edit:
I do have one question though.
What was the source of the myc?
Clone, isolate, MS, etc?


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OfflineChristaphonZ
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Greg]
    #23714517 - 10/06/16 09:39 PM (7 years, 3 months ago)

Thank you for the continued updates. Have the other plates continued this pattern or any anomalies? (Besides different species)


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InvisibleMycolorado
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: ChristaphonZ]
    #23714645 - 10/06/16 10:41 PM (7 years, 3 months ago)

Thanks, Greg! So, these were spores to agar and transferred a couple times(plate in my sig is one).  Then grew it on some grain that had a really crazy red bacteria growing in it.  They were left to sit and fully colonized with pockets of dark red.  Anyway it took a couple transfers from there to clean. I guess they're around The 7th transfer or so. Both came from this:


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InvisibleFriedEggS
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23715141 - 10/07/16 04:57 AM (7 years, 3 months ago)

your experiment is not valid unless you are using a true isolate

but i agree using less nutes will make mycelium spread out more in search of food


Edited by FriedEgg (10/07/16 06:22 AM)


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InvisibleMycolorado
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: FriedEgg]
    #23715372 - 10/07/16 07:14 AM (7 years, 3 months ago)

Quote:

FriedEgg said:
your experiment is not valid unless you are using a true isolate

but i agree using less nutes will make mycelium spread out more in search of food




Huh?  I'm simply looking at the difference in mycelial growth characteristics depending on nutrient content of the media. This has nothing to do with working with a true isolate.  The reason the experiment isn't valid is because there is no control plate.


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InvisibleFriedEggS
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23715408 - 10/07/16 07:24 AM (7 years, 3 months ago)

because you aren't working with the same exact genetics on each plate. different genetics grow at different rates. how do you know one of those plates doesn't have slow genetics and that's why it's growing slow? you don't.

i don't mean to be mean but your experiment is like choosing two random humans of different running abilities run with different types of running shoes to see which shoe makes them run faster. how do you know person #1 doesn't naturally run faster than person #2? you don't. it would be better to have 1 person try out both sets of running shoes.


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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: FriedEgg]
    #23715453 - 10/07/16 07:43 AM (7 years, 3 months ago)

You are correct.  However, this culture has been isolated down a bit and always grows the same on the standard 2% recipe I use...this is the first time I've ever had a growth pattern like that out of this culture.  I also ran the comparison on a variety of commercial edibles that should be isolates and got the same results.  So, I would say those results were fairly conclusive.  But if you want to be concerned with my scientific method and try to poke holes, so be it.  What have you contributed lately?  Jerking off and ringing in the ears?

Here's one with reishi:


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InvisibleFriedEggS
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23715518 - 10/07/16 08:01 AM (7 years, 3 months ago)

don't get your panties in a twist dude. i already said the conclusion you came to was correct, just not the way you went about it.


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OfflineGreg
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: FriedEgg]
    #23715858 - 10/07/16 10:18 AM (7 years, 3 months ago)

That's actually why I asked what the source of the myc was.
7th transfer is probably not far enough to ensure this isn't just genetics.

Ideally you'd have more than one set of cube plates too.


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InvisibleMycolorado
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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Greg]
    #23715952 - 10/07/16 10:49 AM (7 years, 3 months ago)

Quote:

Greg said:
That's actually why I asked what the source of the myc was.
7th transfer is probably not far enough to ensure this isn't just genetics.

Ideally you'd have more than one set of cube plates too.




I'm guessing the results are more associated with the nutrient concentration than the genetics as the results were the same on all the other species I used in the comparison.  Also, did you not read the part where I said this wasn't a good experiment due to the lack of a control and more plates?


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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23728583 - 10/11/16 03:11 PM (7 years, 3 months ago)

Couple pics of the 2%. It's starting to form primordia and significantly so in the crazy rhizo sector.  Definitely appears to be a good fruiter.  I'll get some transfers from that area.


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Re: Comparing growth rate and morphologies of P. Cubensis mycelium on 2% and 4% MEA [Re: Mycolorado]
    #23742521 - 10/16/16 01:14 PM (7 years, 3 months ago)

Here are two more sets of plates taken from the 4% plate in the original comparison. So far, growth patterns are identical to the first round. 
Donor plate:

Transfers:

Original comparison initial growth:


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