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Offlinespore-ty
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Re: Agar [Re: Mad Season]
    #23685783 - 09/28/16 08:28 AM (7 years, 4 months ago)

Im sorry everyone if this is something found easily ive been looking for the answer but cant seem to find it


Should i bring 1000 ml to a boil add my agar and ex. Light malt then strain and then pc?


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Invisiblefilthyknees
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Re: Agar [Re: spore-ty]
    #23685884 - 09/28/16 09:14 AM (7 years, 4 months ago)

I just combine the agar mix and water in the bottle, the pc will homogenize it.


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Offlinespore-ty
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Re: Agar [Re: filthyknees]
    #23686282 - 09/28/16 11:35 AM (7 years, 4 months ago)

Ok thanks waiting for pc to cool down now,


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Offlinemrmazdarx9
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Re: Agar [Re: spore-ty]
    #23686371 - 09/28/16 12:06 PM (7 years, 4 months ago)

Good luck mate:highfive:


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Offlinespore-ty
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Re: Agar [Re: mrmazdarx9]
    #23686563 - 09/28/16 12:57 PM (7 years, 4 months ago)



Waiting for the bottle to cool some more but if someone could answer if this is correct

Im going to wait for the bottle to cool a bit more to be able to handle then i will pour dishes and store in bags until cool, inoculate , then wrap with parafilm?


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OfflineGreg
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Re: Agar [Re: spore-ty]
    #23686600 - 09/28/16 01:05 PM (7 years, 4 months ago)

Trying to get freshly poured dishes back into their sleeve would be a nightmare.
Let them cool/solidify in stacks first before putting them back in the sleeve. I usually do stacks of 8.

Truth be told I don't even put them back in the sleeve and I've never had issues (as long as the dishes stay in the SAB).

If you plan to put them in the fridge then you should put em back in the sleeve for sure.

Other than that you are right!


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Offlinespore-ty
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Re: Agar [Re: Greg]
    #23686622 - 09/28/16 01:12 PM (7 years, 4 months ago)

Quote:

Greg said:
Trying to get freshly poured dishes back into their sleeve would be a nightmare.
Let them cool/solidify in stacks first before putting them back in the sleeve. I usually do stacks of 8.

Truth be told I don't even put them back in the sleeve and I've never had issues (as long as the dishes stay in the SAB).

If you plan to put them in the fridge then you should put em back in the sleeve for sure.

Other than that you are right!





Ya im gonna be knocking up today thank you so much for the quick reply ill just leave them in mini stacks in the sab until inoculation later today


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OfflineGreg
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Re: Agar [Re: spore-ty]
    #23686639 - 09/28/16 01:15 PM (7 years, 4 months ago)

No problem.

Here are some I have had in my SAB for over a week without contams:


Out of the hundreds I've poured only one has contaminated before I used it.


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OfflineGreg
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Re: Agar [Re: Greg]
    #23686651 - 09/28/16 01:17 PM (7 years, 4 months ago)

One other thing, make sure to cut the parafilm in half lengthwise, you don't need the full width to cover a petri:


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Offlinespore-ty
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Re: Agar [Re: Greg]
    #23686674 - 09/28/16 01:22 PM (7 years, 4 months ago)

Can i pre cut a long lengthy piece then cut in sab to size?


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OfflineGreg
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Re: Agar [Re: spore-ty]
    #23686687 - 09/28/16 01:26 PM (7 years, 4 months ago)

I don't see why not.
I cut mine like this outside of the SAB.
2 squares (4 in.) length goes around a regular petri once, 4 squares goes around twice and is what I use.


Sometimes I wrap the plates outside the SAB too, but you have to be careful not to lift the lids at all.


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