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Offlinespore-ty
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Agar
    #23679124 - 09/26/16 08:50 AM (7 years, 4 months ago)

So with my mono giving me some fruits i will take some to agar and i also have a print ill be doing as well, I do have a few questions if anybody doesnt mind answering

Is potato flakes, malt extract and agar ok for a recipe?

I have a pyrex media bottle do i need to loosen the top and put foil on top to p.c?

And finally about how much should i p.c. to pour 80 dishes?

Thanks guys




Edited by spore-ty (09/26/16 08:51 AM)


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Offlinemrmazdarx9
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Re: Agar [Re: spore-ty]
    #23679198 - 09/26/16 09:20 AM (7 years, 4 months ago)

I think its potato or malt with dextrose and agar. My media bottle has a cap rated to 140°C so foil ksnt necessary if yours has the same


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InvisibleMad Season
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Re: Agar [Re: mrmazdarx9]
    #23679204 - 09/26/16 09:24 AM (7 years, 4 months ago)

PDA = potato dextrose agar. MEA = malt extract agar, no dextrose added at all.

They're ingredients for 2 different recipes.

25 ml per plate. 25*80=2000 mL (2 litres)


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Offlinemrmazdarx9
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Re: Agar [Re: Mad Season]
    #23679233 - 09/26/16 09:37 AM (7 years, 4 months ago)

Sorry my bad I only use pda so didnt know, but still its either potato or malt.


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OfflineRhizomorpheus
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Re: Agar [Re: mrmazdarx9]
    #23679241 - 09/26/16 09:41 AM (7 years, 4 months ago)

https://www.shroomery.org/forums/showflat.php/Number/19208976

I like this tek it's one of my favorites.


--------------------
=) All smiles, all equaL (= Anything I post to this forum came from a book. All of it is entirely based in fiction and is for amusement purposes only. The dude hopes you enjoy it as much as he has. It's all just a dreaM.
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Invisiblefilthyknees
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Re: Agar [Re: Rhizomorpheus]
    #23679329 - 09/26/16 10:12 AM (7 years, 4 months ago)

He already has 80 petris, I don't think he needs to make no pour agar too.


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OfflineRhizomorpheus
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Re: Agar [Re: filthyknees]
    #23679382 - 09/26/16 10:27 AM (7 years, 4 months ago)

Oh shit for reals I did not know he had the dishes. I miss understood the post it was just my two cents and one I found helpful when I strarted agar. My bad_
Peace Love Light and Blessings
Edit: I see the pic now I'm on my phone must be blind as a bat:facepalm3:


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=) All smiles, all equaL (= Anything I post to this forum came from a book. All of it is entirely based in fiction and is for amusement purposes only. The dude hopes you enjoy it as much as he has. It's all just a dreaM.
:pipesmoke:


Edited by Rhizomorpheus (09/26/16 10:29 AM)


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InvisibleNumeroEno
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Re: Agar [Re: Rhizomorpheus]
    #23679433 - 09/26/16 10:48 AM (7 years, 4 months ago)

Personally I don't like PDA. I may try making it with reduced sweet potato broth, but I don't like the growth I get on traditional PDA. Dog food and malt extract are my favorite agar nutes.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

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Offlinespore-ty
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Re: Agar [Re: Rhizomorpheus]
    #23679465 - 09/26/16 10:57 AM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
I think its potato or malt with dextrose and agar. My media bottle has a cap rated to 140°C so foil ksnt necessary if yours has the same





Mine is the same so do i just loosen the lid?
Quote:

Mad Season said:
PDA = potato dextrose agar. MEA = malt extract agar, no dextrose added at all.

They're ingredients for 2 different recipes.

25 ml per plate. 25*80=2000 mL (2 litres)





Thanks for the clarification so is dextrose needed in the pda? Or can i do just potato flakes and agar?
Quote:

Rhizomorpheus said:
https://www.shroomery.org/forums/showflat.php/Number/19208976

I like this tek it's one of my favorites.




Very good link thanks ill save for when i do pastys


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Offlinespore-ty
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Re: Agar [Re: NumeroEno]
    #23679472 - 09/26/16 10:59 AM (7 years, 4 months ago)

Quote:

NumeroEno said:
Personally I don't like PDA. I may try making it with reduced sweet potato broth, but I don't like the growth I get on traditional PDA. Dog food and malt extract are my favorite agar nutes.





Lol your the man i was just gonna ask about this, what brand do you use?


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InvisibleMad Season
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Re: Agar [Re: spore-ty]
    #23679474 - 09/26/16 10:59 AM (7 years, 4 months ago)

Dextrose is very much needed for PDA. But yeah I too prefer just malt + agar. So easy. 2% malt and 2% agar. Done.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Offlinespore-ty
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Re: Agar [Re: Mad Season]
    #23679521 - 09/26/16 11:10 AM (7 years, 4 months ago)

So 2000 ml
2% agar - 40 ml
2% malt - 40 ml?


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Offlinemrmazdarx9
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Re: Agar [Re: spore-ty]
    #23679535 - 09/26/16 11:17 AM (7 years, 4 months ago)

Is yours like mine

Why would you losen the lid? I did about 600ml didnt fill to 1000ml but it is designed for this purpose id imagine so I put lid on as tight as it would go and chucked it through a pc cycle works brilliant


--------------------
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InvisibleNumeroEno
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Re: Agar [Re: spore-ty]
    #23679572 - 09/26/16 11:32 AM (7 years, 4 months ago)

Quote:

spore-ty said:
Quote:

NumeroEno said:
Personally I don't like PDA. I may try making it with reduced sweet potato broth, but I don't like the growth I get on traditional PDA. Dog food and malt extract are my favorite agar nutes.





Lol your the man i was just gonna ask about this, what brand do you use?




Same stuff I feed my dog.



--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleMad Season
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Re: Agar [Re: spore-ty]
    #23679578 - 09/26/16 11:34 AM (7 years, 4 months ago)

Quote:

spore-ty said:
So 2000 ml
2% agar - 40 ml
2% malt - 40 ml?



Actually 40 grams of agar and 40 grams of malt. This is just assuming it has a density similar to water, which is highly unlikely. Idk the exact weight 2% is because I don't know the density's, but I always just remember it as 2g of each per 100 ml. Which is 2% lol


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Offlinelovelaughlibs
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Re: Agar [Re: Mad Season]
    #23679585 - 09/26/16 11:37 AM (7 years, 4 months ago)

Quote:

Mad Season said:
Dextrose is very much needed for PDA. But yeah I too prefer just malt + agar. So easy. 2% malt and 2% agar. Done.



I was getting issues with literally no growth for 3 batches of pasty plates before I realised I hadn't added dextrose...I felt stupid when I realised


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Offlinespore-ty
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Re: Agar [Re: mrmazdarx9]
    #23679595 - 09/26/16 11:41 AM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
Is yours like mine

Why would you losen the lid? I did about 600ml didnt fill to 1000ml but it is designed for this purpose id imagine so I put lid on as tight as it would go and chucked it through a pc cycle works brilliant





Only difference is mine is pyrex with orange cap and ok sweet ive never used before thats why


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Offlinemrmazdarx9
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Re: Agar [Re: spore-ty]
    #23679614 - 09/26/16 11:47 AM (7 years, 4 months ago)

It was the first time I used mine held up fine, you should be ok


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COCA GROWERS come here and share your knowledge
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Offlinespore-ty
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Re: Agar [Re: Mad Season]
    #23679804 - 09/26/16 12:53 PM (7 years, 4 months ago)

Quote:

Mad Season said:
Quote:

spore-ty said:
So 2000 ml
2% agar - 40 ml
2% malt - 40 ml?



Actually 40 grams of agar and 40 grams of malt. This is just assuming it has a density similar to water, which is highly unlikely. Idk the exact weight 2% is because I don't know the density's, but I always just remember it as 2g of each per 100 ml. Which is 2% lol





Lol so every 100ml of water 2g's of each?


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InvisibleMad Season
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Re: Agar [Re: spore-ty]
    #23679875 - 09/26/16 01:17 PM (7 years, 4 months ago)

Yes


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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