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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Agar
#23679124 - 09/26/16 08:50 AM (7 years, 4 months ago) |
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So with my mono giving me some fruits i will take some to agar and i also have a print ill be doing as well, I do have a few questions if anybody doesnt mind answering
Is potato flakes, malt extract and agar ok for a recipe?
I have a pyrex media bottle do i need to loosen the top and put foil on top to p.c?
And finally about how much should i p.c. to pour 80 dishes?
Thanks guys
Edited by spore-ty (09/26/16 08:51 AM)
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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I think its potato or malt with dextrose and agar. My media bottle has a cap rated to 140°C so foil ksnt necessary if yours has the same
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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PDA = potato dextrose agar. MEA = malt extract agar, no dextrose added at all.
They're ingredients for 2 different recipes.
25 ml per plate. 25*80=2000 mL (2 litres)
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
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Last seen: 1 year, 4 months
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Sorry my bad I only use pda so didnt know, but still its either potato or malt.
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Rhizomorpheus
PrototypE


Registered: 08/07/16
Posts: 135
Loc: Deep SpacE
Last seen: 4 years, 2 months
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-------------------- =) All smiles, all equaL (= Anything I post to this forum came from a book. All of it is entirely based in fiction and is for amusement purposes only. The dude hopes you enjoy it as much as he has. It's all just a dreaM.
 
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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He already has 80 petris, I don't think he needs to make no pour agar too.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Rhizomorpheus
PrototypE


Registered: 08/07/16
Posts: 135
Loc: Deep SpacE
Last seen: 4 years, 2 months
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Oh shit for reals I did not know he had the dishes. I miss understood the post it was just my two cents and one I found helpful when I strarted agar. My bad_ Peace Love Light and Blessings Edit: I see the pic now I'm on my phone must be blind as a bat
-------------------- =) All smiles, all equaL (= Anything I post to this forum came from a book. All of it is entirely based in fiction and is for amusement purposes only. The dude hopes you enjoy it as much as he has. It's all just a dreaM.
 
Edited by Rhizomorpheus (09/26/16 10:29 AM)
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Personally I don't like PDA. I may try making it with reduced sweet potato broth, but I don't like the growth I get on traditional PDA. Dog food and malt extract are my favorite agar nutes.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
mrmazdarx9 said: I think its potato or malt with dextrose and agar. My media bottle has a cap rated to 140°C so foil ksnt necessary if yours has the same
Mine is the same so do i just loosen the lid? Quote:
Mad Season said: PDA = potato dextrose agar. MEA = malt extract agar, no dextrose added at all.
They're ingredients for 2 different recipes.
25 ml per plate. 25*80=2000 mL (2 litres)
Thanks for the clarification so is dextrose needed in the pda? Or can i do just potato flakes and agar?Quote:
Rhizomorpheus said: https://www.shroomery.org/forums/showflat.php/Number/19208976
I like this tek it's one of my favorites.
Very good link thanks ill save for when i do pastys
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
NumeroEno said: Personally I don't like PDA. I may try making it with reduced sweet potato broth, but I don't like the growth I get on traditional PDA. Dog food and malt extract are my favorite agar nutes.
Lol your the man i was just gonna ask about this, what brand do you use?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Dextrose is very much needed for PDA. But yeah I too prefer just malt + agar. So easy. 2% malt and 2% agar. Done.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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So 2000 ml 2% agar - 40 ml 2% malt - 40 ml?
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Is yours like mine
 Why would you losen the lid? I did about 600ml didnt fill to 1000ml but it is designed for this purpose id imagine so I put lid on as tight as it would go and chucked it through a pc cycle works brilliant
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Quote:
spore-ty said:
Quote:
NumeroEno said: Personally I don't like PDA. I may try making it with reduced sweet potato broth, but I don't like the growth I get on traditional PDA. Dog food and malt extract are my favorite agar nutes.
Lol your the man i was just gonna ask about this, what brand do you use?
Same stuff I feed my dog.
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
spore-ty said: So 2000 ml 2% agar - 40 ml 2% malt - 40 ml?
Actually 40 grams of agar and 40 grams of malt. This is just assuming it has a density similar to water, which is highly unlikely. Idk the exact weight 2% is because I don't know the density's, but I always just remember it as 2g of each per 100 ml. Which is 2% lol
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
Last seen: 5 years, 11 months
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Quote:
Mad Season said: Dextrose is very much needed for PDA. But yeah I too prefer just malt + agar. So easy. 2% malt and 2% agar. Done.
I was getting issues with literally no growth for 3 batches of pasty plates before I realised I hadn't added dextrose...I felt stupid when I realised
-------------------- Ask and ye shall receive; Seek and ye shall find.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
mrmazdarx9 said: Is yours like mine
 Why would you losen the lid? I did about 600ml didnt fill to 1000ml but it is designed for this purpose id imagine so I put lid on as tight as it would go and chucked it through a pc cycle works brilliant
Only difference is mine is pyrex with orange cap and ok sweet ive never used before thats why
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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It was the first time I used mine held up fine, you should be ok
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
Mad Season said:
Quote:
spore-ty said: So 2000 ml 2% agar - 40 ml 2% malt - 40 ml?
Actually 40 grams of agar and 40 grams of malt. This is just assuming it has a density similar to water, which is highly unlikely. Idk the exact weight 2% is because I don't know the density's, but I always just remember it as 2g of each per 100 ml. Which is 2% lol
Lol so every 100ml of water 2g's of each?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Yes
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