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Offlineformula_raider
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Registered: 07/17/13
Posts: 9
Last seen: 7 years, 4 months
Having extreme difficulity getting Penis Envy to pinset in Monotub
    #23677790 - 09/25/16 07:51 PM (7 years, 4 months ago)

I was hoping I could get some advice on figuring out why my strand of Penis Envy is not pinsetting in the mono-tub.

Any help is greatly appreciated as I don't want to loose the batches that I have and that are coming. 

This is what I have:
Penis Envy inoculated in (10) half-pint jars of WBS. Was taking over the bird seed quite nicely and completely consumed it after 20 days.
temperature @ 77-79. No contamination in jars, all looked great.

Spawned to bulk in mono-tub 66qt. Coir (1-brick), Verm (1 qt), Horse Manure (1.5qt), water (5 qts), Gypsum Pasturized in bag @140-155 for 1 hour.

Left in darkness (13) more days where it completely covered all the substrate. Everything looked very good, no contamination, nice water beading on the sides and like water droplets on the substrate. 

Birthed on the 13th day with 12-hr on-off lighting; with 1-2 box fans blowing in the far side of the same room but never directly at tub. (4) bottom holes of mono-tub stuffed pretty thick, (2) top holes very loosely. temp @78-80.

It is now 2-weeks after birth and I am getting no pinset where usually on other strains I get them in about 7-10 days.
Just out of comparison, right next to it is a tub of Mexi-Cub which the substrate is exactly the same, birthed at the same time and under all the same conditions and is growing just fine. 

I am getting these weird alien growths that are mostly in the corners?!  They are freaking me out but no pins! I am afraid I am going to loose this whole batch but I am clueless on what this needs as all my other grows have worked under these same conditions. 
What am I doing wrong? 

Thanks for any help. Pictures included.








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OfflineKenetic
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Re: Having extreme difficulity getting Penis Envy to pinset in Monotub [Re: formula_raider]
    #23677809 - 09/25/16 07:55 PM (7 years, 4 months ago)

Those are just pe blobs, which are active and edible.  So your mono is actually fruiting.
Just wait, the "regular" shrooms will show up soon enough.  P.E. takes forever


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Having extreme difficulity getting Penis Envy to pinset in Monotub [Re: Kenetic] * 1
    #23678317 - 09/25/16 10:52 PM (7 years, 4 months ago)

Try casing to cut down the blobs next time. But you have loads of primordia. It actually all looks normal for PE.


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OfflineMush Freak
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Registered: 12/29/11
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Re: Having extreme difficulity getting Penis Envy to pinset in Monotub [Re: Pastywhyte]
    #23678400 - 09/25/16 11:25 PM (7 years, 4 months ago)

I've heard casing your substrate helps cut down on mutant blobs when growing penis envy. Try that next time you put a tub into fruiting.


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:mushroom2::mushroom2:Mono-tubs are LIFE:mushroom2::mushroom2:



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InvisibleInocuole
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Re: Having extreme difficulity getting Penis Envy to pinset in Monotub [Re: Mush Freak]
    #23678693 - 09/26/16 03:58 AM (7 years, 4 months ago)

It can also result in more blobs than before.  I don't know that anything necessarily stops blobs altogether other than getting a culture that doesn't do it.


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Offlineformula_raider
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Registered: 07/17/13
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Re: Having extreme difficulity getting Penis Envy to pinset in Monotub [Re: Inocuole]
    #23681609 - 09/26/16 10:57 PM (7 years, 4 months ago)

Thanks guys, I will at least try a test casing next time to see if it helps any. 
So for now I guess I will just sit and wait and hope for the best.


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Having extreme difficulity getting Penis Envy to pinset in Monotub [Re: formula_raider]
    #23682443 - 09/27/16 08:14 AM (7 years, 4 months ago)

I prefer 1/2 inch casing + lots of fresh air for no blobs. 
PE wants a low nutrient substrate to pin off of(low nutrient casing like coir/verm whatever) and oxygen, otherwise you get blobs.

You get blobs on first flush because there is a ton of nutrition to digest on the first flush, and consequently it
takes more oxygen to digest it, so your starving for air first flush in a mono that is tightly stuffed.  Second flush
calms down and the oxygen level goes way up. 
Medium stuffed poly you can get away with with right genetics like azur does. 
Different cultivars of PE have different tolerances for low oxygen levels. 

PE will produce normal looking fruits with maybe a tiny wrinkle in the neck if the oxygen level is high enough.
It will also produce THICK dark prints (aka normal prints) every time with enough oxygen. 
Fruit them outdoors if you want to see for yourself.

I'm 99.99 percent sure this answer is rock solid.


Edited by invitro (09/27/16 08:22 AM)


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OfflineMush Freak
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Registered: 12/29/11
Posts: 154
Last seen: 5 years, 9 days
Re: Having extreme difficulity getting Penis Envy to pinset in Monotub [Re: invitro]
    #23683206 - 09/27/16 01:25 PM (7 years, 4 months ago)

The tub in my signature is MS, I had no blobs and a decent pinset,  I was totally lucky I suppose.  Once you do get some fruits going,  select a good shroom,  preferably one from a nice cluster,  and start working it on agar.  This is probably your best bet to get a stable,  non blob culture.


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:mushroom2::mushroom2:Mono-tubs are LIFE:mushroom2::mushroom2:



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