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Offlineglobejs
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Registered: 08/18/07
Posts: 414
Last seen: 5 months, 19 days
Isolated?
    #23674483 - 09/24/16 04:25 PM (7 years, 6 months ago)

So here's the story of these 2 plates.

Ms to rye berry quart jar.

After growth grew out about an inch I took a rye berry from the leading edge of the best growth from 2 spots and placed those rye berries in the center of 2 dishes.

After those grew out about an inch (like 6 or so days) I took very small pieces from the best growth and transferred to new plates

These are those plates.



My question is if someone can show me any sectoring as I read it would be impossible for me to have an isolate yet but they look pretty uniform.


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OfflineMushierage
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Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 3 months
Re: Isolated? [Re: globejs]
    #23674510 - 09/24/16 04:34 PM (7 years, 6 months ago)

It's hard to see any sectoring with those pics.  It could be isolated or very few strains, but it's hard to judge with the light reflecting off the plate.


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Offlineenlightenment
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Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 10 months, 19 days
Re: Isolated? [Re: globejs]
    #23674511 - 09/24/16 04:35 PM (7 years, 6 months ago)

Looks like an isolate. There are no visible sectors anymore IMO.
Would be big luck to get an isolate by just picking a grain from an MS jar but it is possible.

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InvisibleBoogieman47
Let's boogie
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Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
Re: Isolated? [Re: enlightenment]
    #23674606 - 09/24/16 05:01 PM (7 years, 6 months ago)

If you make another transfer it will prove an isolate or not looks like one to me beautiful plates


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 5 months
Re: Isolated? [Re: Boogieman47]
    #23674737 - 09/24/16 05:48 PM (7 years, 6 months ago)

Those look very good! Congratz. Hope you succeeded.


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InvisibleMycolorado
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Registered: 07/23/16
Posts: 8,567
Loc: Interdimensional Bootcamp
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Re: Isolated? [Re: Tira]
    #23674899 - 09/24/16 07:02 PM (7 years, 6 months ago)

Sure looks that way, nice cultures globejs!  What's the nutrient content of those plates?


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