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OfflineWillHill
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A Newbies trials and error's
    #23672921 - 09/24/16 02:42 AM (7 years, 4 months ago)

So a friend gave me an old liquid culture...

My first growth was kind of exciting..


Until it grew out a bit more.



So I transferred the white section top left to a few fresh dishes


But I'm kind of concerned that Im just caring for some random mould instead of what I was told was Puerto Rican mushroom mycelium!


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InvisibleBoogieman47
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Re: A Newbies trials and error's [Re: WillHill]
    #23672927 - 09/24/16 02:50 AM (7 years, 4 months ago)

Looks like some weird growth to me?? Do a sandwich cut a piece of agar out put the piece inside the hole then cover it up with another piece of agar the myc will grow through the agar


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OfflineWillHill
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Re: A Newbies trials and error's [Re: WillHill]
    #23672928 - 09/24/16 02:53 AM (7 years, 4 months ago)

So I found the smallest amounts of spores left on some tin foil that was wrapped in a plastic baggie and set them out



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OfflineWillHill
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Re: A Newbies trials and error's [Re: Boogieman47]
    #23672929 - 09/24/16 02:56 AM (7 years, 4 months ago)

Quote:

noob47 said:
Looks like some weird growth to me?? Do a sandwich cut a piece of agar out put the piece inside the hole then cover it up with another piece of agar the myc will grow through the agar




Thank you, for this advice I had no idea about this test, ill get to it later tonight!


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Offlinewtfcrazymofo
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Re: A Newbies trials and error's [Re: WillHill]
    #23672934 - 09/24/16 03:02 AM (7 years, 4 months ago)

Quote:

WillHill said:




It was the thought that counts.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: wtfcrazymofo]
    #23672956 - 09/24/16 03:52 AM (7 years, 4 months ago)

You should use a loop or swab not just scrape/sprinkle your spores on your dish. Looks like far to many increasing your contams chances


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OfflineTira
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Re: A Newbies trials and error's [Re: WillHill]
    #23673027 - 09/24/16 04:59 AM (7 years, 4 months ago)

Quote:

WillHill said:







That does not look like cubensis mycelium.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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Offlinewtfcrazymofo
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Re: A Newbies trials and error's [Re: Tira]
    #23673030 - 09/24/16 05:01 AM (7 years, 4 months ago)

Quote:

Tira said:
Quote:

WillHill said:







That does not look like cubensis mycelium.



is that written dirty?


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineMushierage
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Re: A Newbies trials and error's [Re: wtfcrazymofo]
    #23673098 - 09/24/16 06:36 AM (7 years, 4 months ago)

Agreed.  If you haven't already, look up Mad Seasons guide to agar-ing with a smile.

https://www.shroomery.org/forums/showflat.php/Number/21889950

Read this and adhere to it, it'll improve your agar technique immensely dood.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineWillHill
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Re: A Newbies trials and error's [Re: wtfcrazymofo]
    #23674518 - 09/24/16 04:36 PM (7 years, 4 months ago)

Quote:

wtfcrazymofo said:
Quote:

Tira said:
That does not look like cubensis mycelium.



is that written dirty?




OK Im going to try the sandwich just for practice but I guess ill have to accept that was a trial run!

And Yes it came from a dirty dish so i marked it as "drty"


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OfflineWillHill
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Re: A Newbies trials and error's [Re: WillHill]
    #23674558 - 09/24/16 04:49 PM (7 years, 4 months ago)

My sprinkled spores from the foil marked Puerto Rican have started to go white



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OfflineWillHill
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23674697 - 09/24/16 05:33 PM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
You should use a loop or swab not just scrape/sprinkle your spores on your dish. Looks like far to many increasing your contams chances





I totally agree, what size loop should I get, the shop here has from 1.5mm all the way up to 6mm.

They also stock plastic disposable ones but I guess thats not good for the environment


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OfflineKenetic
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Re: A Newbies trials and error's [Re: WillHill]
    #23675395 - 09/24/16 10:13 PM (7 years, 4 months ago)

It doesn't matter what size.


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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: Kenetic]
    #23675770 - 09/25/16 03:09 AM (7 years, 4 months ago)

:whathesaid: your basically only using a speck so size isn't really an issue I suppose a thicker wire will pick up more per dab, but I doubt it would really make that much of a problem


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OfflineWillHill
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23675846 - 09/25/16 04:48 AM (7 years, 4 months ago)

Well the sandwich as suggested is underway but i dont expect it to grow out



and the dish that i thought just had the spores on has started growing allot more than just the spores, the spores have gone bright white but then theres other dots of light grey all over the place, that inoculating loop as suggested I bet would have stopped this!



Edited by WillHill (09/25/16 04:51 AM)


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OfflineTira
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Re: A Newbies trials and error's [Re: WillHill]
    #23675851 - 09/25/16 04:54 AM (7 years, 4 months ago)

Quote:

WillHill said:
and the dish that i thought just had the spores on has started growing allot more than just the spores, the spores have gone bright white but then theres other dots of light grey all over the place, that inoculating loop as suggested I bet would have stopped this!





That's the idea .)


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: Tira]
    #23675858 - 09/25/16 05:03 AM (7 years, 4 months ago)

The idea with the first plate is to transfer the myc growth before the contams can grow out. So if you do get some confirmed myc growth cut it out and transfer it straight away don't let it grow out.


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OfflineWillHill
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23675920 - 09/25/16 06:18 AM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
The idea with the first plate is to transfer the myc growth before the contams can grow out. So if you do get some confirmed myc growth cut it out and transfer it straight away don't let it grow out.




I can still see some of the bigger bits of the dark colored spores, they have a ring of pure white around them, other smaller spots of spores are now the same pure white, i guess i should transfer them now even though they are only like 1-2mm in diameter??


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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: WillHill]
    #23675925 - 09/25/16 06:25 AM (7 years, 4 months ago)

Pics first if your not sure but I can't see how you'd have much in a day


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OfflineWillHill
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23676248 - 09/25/16 10:07 AM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
Pics first if your not sure but I can't see how you'd have much in a day




Yeah i really dont have much at all but i can see these white spots are from the visible spores i dropped, the other not so white areas did not have visible spores that they appeared from



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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: WillHill]
    #23676295 - 09/25/16 10:28 AM (7 years, 4 months ago)

Spores take longer than a day usually. They'll need to grow out more so you can actually separate growth from the original spores germination point
This is the peu I took a transfer from at this size which was a little later than I needed to leave it

This would have been the better time to take it but didn't have time to do it


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Edited by mrmazdarx9 (09/25/16 10:36 AM)


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InvisibleMycolorado
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Re: A Newbies trials and error's [Re: WillHill]
    #23676306 - 09/25/16 10:33 AM (7 years, 4 months ago)

Quote:

WillHill said:
Quote:

mrmazdarx9 said:
Pics first if your not sure but I can't see how you'd have much in a day




Yeah i really dont have much at all but i can see these white spots are from the visible spores i dropped, the other not so white areas did not have visible spores that they appeared from






Hey WillHill,

There is NO WAY those are cubensis spores germinating on that plate.  When you tapped out the "spores" on the plate you were inoculating it with everything else that was on the foil, in the bag, etc.  You should chuck all that shit and start over with known clean spores or tissue.  It will save you a lot of time, resources and frustration.


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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


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OfflineWillHill
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Re: A Newbies trials and error's [Re: Mycolorado]
    #23676574 - 09/25/16 12:09 PM (7 years, 4 months ago)

Thank you for your incredibly direct comments, it's what I really needed to hear, I really am just wasting my time here.

I have ordered up a fresh spore print and a loop, is there anything else I should get in preparation for a fresh start?

When spores do arrive, any advice for getting them from the foil to the agar?


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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: WillHill]
    #23676594 - 09/25/16 12:15 PM (7 years, 4 months ago)

Do all work in SAB follow sterile techniques iso, gloves, flame loop, spray SAB soapy water etc. Literally touch the print with the loop then swipe/dab it on plate. Be careful but quick, I'm not saying move fast I'm saying have your movements planned and everything arranged in SAB where it needs to be its a good idea to do a dry run just so you know where things need to be so your not scrabbling around with the petri lid off trying to grab something you put behind your arm or something. That's what I do anyway


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OfflineWillHill
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23676613 - 09/25/16 12:22 PM (7 years, 4 months ago)

It will be at least a week maybe 2 before they get here, I'm going to use this time to build a low cost HEPA filter and fan setup.

I made a quick solid works drawing..



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Re: A Newbies trials and error's [Re: WillHill]
    #23676626 - 09/25/16 12:26 PM (7 years, 4 months ago)

You bet!  Flame your loop till glowing red.  You shouldn't need to cool it in the agar as loops cool off pretty quickly...then gently drag the loop across a small section of the print and then gently drag your loop back across the agar.  All this should be done in a still air box or in front of a hepa filter of course.  Definitely follow up on sterile technique and so on as mentioned above.  You'll be very happy with your results if you do some real research on agar, technique, etc. and apply what you've studied.  Your spores will germinate around 5-7 days, typically.  Good luck!


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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: WillHill]
    #23676639 - 09/25/16 12:31 PM (7 years, 4 months ago)

Quote:

WillHill said:
It will be at least a week maybe 2 before they get here, I'm going to use this time to build a low cost HEPA filter and fan setup.

I made a quick solid works drawing..





If it works well and is affordable it would be very interesting to see the main thing that's put me off is the cost of a flow hood but I'd love to have one


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OfflineWillHill
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23676666 - 09/25/16 12:41 PM (7 years, 4 months ago)

This I've designed at 50cm wide x 40cm deep for the footprint on the desk, i dont have much space to give it more and including the 99.9% filter, fan, germicidal bulb, regular strip light and plexi glass housing and a few buttons I'll give it a budget of 200 dollars.

Should be a decent challenge, the hardest bit will be mounting the fan and impeller on a bearing. I'll order up the plexi now.


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OfflineWillHill
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Re: A Newbies trials and error's [Re: WillHill]
    #23677733 - 09/25/16 07:30 PM (7 years, 4 months ago)

Check out this, mess, this is from tapping spores onto a dish



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OfflineKenetic
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Re: A Newbies trials and error's [Re: WillHill]
    #23677754 - 09/25/16 07:39 PM (7 years, 4 months ago)

Why?  Looks like the milky way


--------------------
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OfflineWillHill
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Re: A Newbies trials and error's [Re: Kenetic]
    #23677767 - 09/25/16 07:44 PM (7 years, 4 months ago)

Quote:

kenetic said:
Why?  Looks like the milky way




I think its important for others newbies like me to know what happens if they dont do things the right way


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OfflineTira
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Re: A Newbies trials and error's [Re: WillHill]
    #23678704 - 09/26/16 04:12 AM (7 years, 4 months ago)

Quote:

WillHill said:
I think its important for others newbies like me to know what happens if they dont do things the right way




That's right .) Good thinking


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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OfflineWillHill
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Re: A Newbies trials and error's [Re: Tira]
    #23678875 - 09/26/16 06:26 AM (7 years, 4 months ago)

I got the case laser cut today and then drilled and tapped the screw holes.

12mm thick acrylic, lets see how easily it scratches over time, I may swap the table out for stainless steel one

I'm confident that the 316 stainless M5x30 screws will never rust.





Just waiting on the other parts now


Edited by WillHill (09/26/16 06:32 AM)


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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: WillHill]
    #23678879 - 09/26/16 06:33 AM (7 years, 4 months ago)

Yeah man that looks cool


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OfflineWillHill
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23680650 - 09/26/16 06:06 PM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
Spores take longer than a day usually. They'll need to grow out more so you can actually separate growth from the original spores germination point
This is the peu I took a transfer from at this size which was a little later than I needed to leave it




Why do we need to separate growth from the spore? What happens if we dont?


Edited by WillHill (09/26/16 06:08 PM)


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OfflineWillHill
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Re: A Newbies trials and error's [Re: WillHill]
    #23681114 - 09/26/16 08:06 PM (7 years, 4 months ago)

The filter arrived this morning, the sticker says its rated at 99.995%



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Re: A Newbies trials and error's [Re: WillHill]
    #23681159 - 09/26/16 08:21 PM (7 years, 4 months ago)



--------------------
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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


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OfflineWillHill
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Re: A Newbies trials and error's [Re: Mycolorado]
    #23685444 - 09/28/16 05:15 AM (7 years, 4 months ago)

Can anyone let me know if there is any benefit to adding a UV Germicidal / Antibacterial Sterilizer light inside my home made flow hood?


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OfflineKenetic
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Re: A Newbies trials and error's [Re: WillHill]
    #23685447 - 09/28/16 05:20 AM (7 years, 4 months ago)

Seems redundant.


--------------------
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OfflineWillHill
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Re: A Newbies trials and error's [Re: Kenetic]
    #23685467 - 09/28/16 05:34 AM (7 years, 4 months ago)

Quote:

kenetic said:
Seems redundant.




Thanks for that, I just saw them in allot of commercial flow hoods, I guess the idea is to turn it on before use for a while to kill anything on the inside of the hood. But as its redundant ill not bother adding one.


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Re: A Newbies trials and error's [Re: WillHill]
    #23685599 - 09/28/16 07:08 AM (7 years, 4 months ago)

Then your just removing the same spores? I dont get what you mean spore on agar transfer from the clean growth onto new agar plate.


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OfflineWillHill
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23685628 - 09/28/16 07:28 AM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
Then your just removing the same spores? I dont get what you mean spore on agar transfer from the clean growth onto new agar plate.




Maybe I miss understood what you were trying to get across to me when you said..

Quote:

mrmazdarx9 said:
They'll need to grow out more so you can actually separate growth from the original spores germination point




I thought you meant that that once a spore germinates you have to separate the growth from the point where the spore was on the dish??? An I was wondering what happens if youdont does that point where the spore was get contaminated or???


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InvisibleMycolorado
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Re: A Newbies trials and error's [Re: WillHill]
    #23686952 - 09/28/16 02:27 PM (7 years, 4 months ago)

Typically, once the spores have germinated, people will transfer to a new plate.  From there you can continue to transfer the stronger growths to new plates, isolating that strong growth, usually the rhizomorphic (ropey looking)sectors. Then, when you have a few different areas isolated down on different plates, you can expand and fruit each of them to pick the best performer......read up.


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Re: A Newbies trials and error's [Re: Mycolorado]
    #23686969 - 09/28/16 02:30 PM (7 years, 4 months ago)

this is why I worry about agar, it gets you amazing spawn but its just waaaay to complicated for beginners


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Re: A Newbies trials and error's [Re: acidninja]
    #23687196 - 09/28/16 03:26 PM (7 years, 4 months ago)

Agar work IS NOT COMPLICATED.  I began working with fungi on agar as a beginner.  Agar work opens up a whole new area of cultivation and experimentation.  It enables you to get away from multispore grows and to begin working with clones and to clean up contaminated specimens.  Once you do a bit of reading and actually start to play with agar, you'll find it to be very easy and you'll wonder why you didn't jump into it sooner.


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Offlinemrmazdarx9
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Re: A Newbies trials and error's [Re: acidninja]
    #23689403 - 09/29/16 06:35 AM (7 years, 3 months ago)

Nothing hard about agar, make plate add spores, cut out best bit add to new plate .


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OfflineGreenskybluegrass7
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Re: A Newbies trials and error's [Re: mrmazdarx9]
    #23689565 - 09/29/16 07:44 AM (7 years, 3 months ago)

I've been messing with agar for a couple weeks now and it is very simple.  Don't be intimidated, you can clearly see if theres a contamination.  Just follow the teks


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