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OfflineShineonyoucrazy
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Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Best way to go forward from my mw kit buying mistake.
    #23670301 - 09/23/16 07:44 AM (7 years, 4 months ago)

I bought a mw growkit mega kit a few weeks ago, along with a few different spore syringes from two site sponsors (at least i got that right, I'm learning). I now know from reading and researching that the kit was a bust, with a bunch of unnecessary crap. The jars were my main concern on this attempt being the first ever on my part, and not having necessary time and equipment to make my own right now. That's next on my list of things to do, along with just jumping to some grain jars and substrate from outgrow and going for the monotub/tray bulk methods.  I've read positive quite ups from members on here about their pf 1/2 pint brf jars.  What would be the best way to go on from here? I inoculated the jars on 9/19 and they are in the tub at a constant 76F. Would it be best to say screw the kit stuff and just make a sgfc for any cakes that make it to birth?  Any guidance would be appreciated. Thank you for your time!


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Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23670338 - 09/23/16 08:03 AM (7 years, 4 months ago)

.


Edited by amidogen (04/19/18 10:23 AM)


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OfflineShineonyoucrazy
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Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23670625 - 09/23/16 10:21 AM (7 years, 4 months ago)

Thank you! I ordered it on a whim after looking over the forms here and there, but a soon as i did i started reading and realized about the only thing that may be of use is the jars, gloves, and tub lol.  The damage was done though, but the more i read the more i was sucked in and wanted to do this right. 76F is the tops they have been kept at. From what I've understood it seemed to be what was recommended, but it wasn't really explained if that was internal or ambient air.  I know anything over 80 is asking to grow contaminants. Sound i drop the temp and shoot for 75 ish internal temp or ambient air temp, and if so that would ve around 70 to 72 correct? I'm going to go out tonight or this weekend for another tub to turn into the sgfc. Just wanna get things in order so I'm not caught off guard if the jars all colonize without contamination.


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Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23671416 - 09/23/16 03:26 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:23 AM)


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OfflineMadSeasonStudent
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23671539 - 09/23/16 04:05 PM (7 years, 4 months ago)

Why is it a bust because you bought a kit?
Just because someone doesnt think it was wise?
Id use it with confidence, not using it would be unwise.

I bought all the wrong stuff too, mainly on jars.
I always say embrace this time as a grower, because it goes by super fast.


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: MadSeasonStudent]
    #23671561 - 09/23/16 04:13 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:23 AM)


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OfflineShineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23673422 - 09/24/16 09:55 AM (7 years, 4 months ago)

I plan on salvaging what i can for sure. Like i said the damage is done, so it would be crazy to just set it all aside and give up.  Mainly is just that after really diving into further reading, the kit is crap. There are so many extra pieces like air pump/hepa filter, the wrong spectrum lighting, a humidifier, heating pad, etc. All unnecessary,but before actually reading and taking this a bit more seriously, seemed like an ok idea. Another reason now i question it is i inoculated jars 9/19 and half appear contaminated. So i dont know the quality of the process they used in making said jars. The ms spore syringe came from a sponsor, i built a sab, and followed sterile procedures to the t. I just rather learn the right way to do it without the extra cost,or having to rely totally on others work practices.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineShineonyoucrazy
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Registered: 09/22/16
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23673436 - 09/24/16 10:02 AM (7 years, 4 months ago)

Amidogen i also dropped the ambient air temp to 71, and took all the suspect jars and isolated them from the others in a small bin. Im waititng to give them time because it was only inoculated 9/19 and its hard to tell if its the way the light is hitting or if its the start of a contam.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


Edited by Shineonyoucrazy (09/24/16 10:03 AM)


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23673714 - 09/24/16 11:41 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:23 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. *DELETED* [Re: amidogen]
    #23673759 - 09/24/16 11:55 AM (7 years, 4 months ago)

Post deleted by Shineonyoucrazy

Reason for deletion: Double post links not working



--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23673787 - 09/24/16 12:01 PM (7 years, 4 months ago)

Here are some suspect jars


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineShineonyoucrazy
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Registered: 09/22/16
Posts: 917
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23673882 - 09/24/16 12:44 PM (7 years, 4 months ago)

Any guidance on how course the vermiculite should be.  My search on here gets mixed results.  I'm thinking medium so it doesn't pack to tight, and so i can use extra for dunk and roll.  Anyone else have input.  Teks are never explicit on this.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23674206 - 09/24/16 02:42 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:23 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23674391 - 09/24/16 03:50 PM (7 years, 4 months ago)

I apologise about the double post.  I'm brand new to the online thing as well. I didn't know if the pics on the first post worked, so i took the next. 1/2 pint wide mouth, just up close to try and show what was going on.  I'm very uncertain,hence isolating them and just keeping an eye on what's going on. I'd hate to toss a good jar.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23674398 - 09/24/16 03:53 PM (7 years, 4 months ago)

I did just go out shopping.  Got a 16 quart pressure cooker, jars, vermiculite, organic brown rice flour, and more perlite since the kit only came with a large freezer bag full.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23674408 - 09/24/16 03:57 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:24 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23674432 - 09/24/16 04:09 PM (7 years, 4 months ago)

I went back and hit edit post and deleted the post. It just asked i give a reason which i stated double post links not working.  And the shopping list was an after thought if that's what your taking about.  I'm Internet retarded


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineShineonyoucrazy
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Posts: 917
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23674461 - 09/24/16 04:19 PM (7 years, 4 months ago)

Question on the half pint jars. Will those be OK? I looked everywhere around for short little fat ones, but the only short 1/2 pint jars test that were wide mouth rounded at the top of the jar like a regular size jar. I figured birthing the cake would be next to impossible to do in one piece. That's why i went with the tapered ones like this.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23674486 - 09/24/16 04:26 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:24 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23674540 - 09/24/16 04:45 PM (7 years, 4 months ago)

I get what your saying, keeps bumping mine to the top. I don't wanna screw anyone else out from getting their questions answered.  I appreciate you being patient with me and the advice.  I have 4 10ml syringes left. Some are going to noc a all in one bag that was purchased before, but that came from a trusted vendor on this site (purchased same time as the kit which was not a vendor from here). But i think i may give a go with these jars, and order some shorties from the net. All the let's grow mushroom vids i watched before look liked the same jars, but if i can find short, wide,and tapered i can see that being benificial. I didn't like how the ones i found that were short looked like they would give you hell birthing. I also will be taking a look at the agar process you recommended, but that may have to wait a bit. The pressure cooker took most of the rest of the cultivation fund,but seemed like a solid investment.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23674723 - 09/24/16 05:44 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:24 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23675249 - 09/24/16 09:00 PM (7 years, 4 months ago)

Just put together my first 5 jars! They're in the pc right now. Man i really wish i would have done this from the start. I could make jars for years for how much i dropped on the bs kit. They look really nice,fluffy,and a nice 1/2 inch dry vermiculite layer on top. Can't wait till tomorrow to give them a go.  I took your advice, and started small so i have plenty ms to go around for the coming tries and also some agar work in the near future. I popped a premade jar open to see what it was like, seemed like a rock.  I don't have high hopes for the kit jars, but making my own gives me some hope. Could that be why I'm not seeing growth on the other non contam jars.  Too tightly packed?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineKenetic
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23675313 - 09/24/16 09:36 PM (7 years, 4 months ago)

Quote:

Shineonyoucrazy said:
Here are some suspect jars




Normally innocent until proven guilty, but those jars are well beyond suspect.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineShineonyoucrazy
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Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
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Re: Best way to go forward from my mw kit buying mistake. [Re: Kenetic]
    #23675336 - 09/24/16 09:44 PM (7 years, 4 months ago)

The suspect jars were from a mw growkit. I went out and got a pressure cooker today and other supplies to make my own following the pf tek 2:1:1 ratio for making brf cakes.  The ones from the kit had at least an inch dry verm layer and super condensed.  Could that be inhibiting the ones that aren't showing growth?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineKenetic
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23675359 - 09/24/16 09:59 PM (7 years, 4 months ago)

I started with pf tek and learned that you shouldn't pack the sub too dense because it doesn't allow for air pockets to penetrate the sub.  During shipping your jars probably got messed up and became too dense or something. 
One inch of verm on top isn't going to cause a problem, but a "super condensed" sub will.  After you mix the ingredients, loosely put them in the jar and apply the verm layer on top.  inoculate and put on a shelf until they're done.  Don't mess with them too much

I'd just follow the pf tek as it is, but keep in mind you don't want to compact a pf cake.

edit:  changed their to they're


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Edited by Kenetic (09/24/16 10:06 PM)


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OfflineShineonyoucrazy
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Registered: 09/22/16
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Re: Best way to go forward from my mw kit buying mistake. [Re: Kenetic]
    #23675393 - 09/24/16 10:12 PM (7 years, 4 months ago)

Awesome! That makes me feel good about the jars i just made myself then. The radio seemed nice (squeezed the vermiculite and water mixture and just a few drops came out like the tek said, scooped the brf mixture into the jars making sure not to pack them, and gave it a nice 1/2 dry layer, then they went into the pc. They are cooling now in the pc after a little more than 60 mins in @15psi, and I'll try inoculating them tomorrow morning when they cool. All in all i have better hopes for my homemade jars.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineKenetic
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23675398 - 09/24/16 10:15 PM (7 years, 4 months ago)

You are on your way to success.  If you fail, try again.  and again if you must.  You'll get it right soon with that attitude.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
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Re: Best way to go forward from my mw kit buying mistake. [Re: Kenetic]
    #23675417 - 09/24/16 10:25 PM (7 years, 4 months ago)

Thank you! Definatly bound and determined to get it right, so it's only a matter of patients,time,practice, and of course continued reading and research.  I'm definatly ok with all of that though. I needed a fun rewarding hobby, and even though it's very early this seems like this is on its way to becoming just that. This is a wonderful thing,and hopefully someday I'll be knowledgeable enough to pass the torch like so many of you guys are. Before this i worked on mimosa and acacia extractions, so this is a fun jump.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineKenetic
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23675464 - 09/24/16 10:57 PM (7 years, 4 months ago)

Nice!  Hobbies are cool.  I have so many I can't master any of them.  Or afford them, or make time for them.  Luckily mushies don't take up a lot of my time (unless I'm on a forum) so it it definitely rewarding.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23676053 - 09/25/16 08:17 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:24 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23676166 - 09/25/16 09:31 AM (7 years, 4 months ago)

Thanks amidogen! I'm lucky to have a pretty full schedule outside of this that keeps me busy in the time between.  I picked up the pc yesterday and supplies and made my first run of jars.  I figure is would be a great investment that could open a lot of avenues here to branch out.  I like the idea you have to spreading your time around/not putting your eggs all in one basket.  Next check i want to get the supplies for the no pour agar tek and try working with the ms in that to clean and isolate.  I have a question. Out of the 18 jars i inoculated 9 are definatly contaminated at the point of inoculation.  Those were inoculated with one spore stringe and all the non contaminated jars with another. They both came from site vendors, but the contaminated jar syringe came from a vendor i didn't tell i was a member from here.  Do you think it was the syringe? All jars were inoculated in a sab using sterile procedures, flaming the needle between jars, etc. I just find it odd only those jars (all) have contamination using the exact procedure at the same time. Thoughts?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23676178 - 09/25/16 09:41 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:24 AM)


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OfflineShineonyoucrazy
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Posts: 917
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23676283 - 09/25/16 10:21 AM (7 years, 4 months ago)

*Yes two different syringes from two different vendors

*Yes they are both shroomery vendors from the sponsor list

*The contaminated jars came from a syringe i did not tell the vendor i was a member

*The non contaminated jars came from a syringe i told the vendor i was a member (also got a freebie for being a member)

In total i have 2 left from the non contaminated jar vendor (PE and PSEA) and 1 from the contaminated jar vendor left (golden teacher)

I'm just up in the air about using the second syringe from the possibly bad set. In all fairness the jars weren't mine and i kept them in a tub in the closet at 76F (no heater just the tub kept that temp in the closet) with the foil lightly over the top until i learned that was wrong. Do you think the lack of proper ge/temps did it? I just find it odd that it's only those jars, but the ms from one could of been cleaner than the other.  I've read no ms is 100% clean which is why people usually go agar to clean it up first for teks other than brf.

Also the foil and temp combo could be why the ones not showing contamination, but also no growth could be slow right? When we talked a couple days ago and i said i fixed the temp, i took them all out and foil off the top. I hope that kicks the growth into gear.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23676298 - 09/25/16 10:29 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23676322 - 09/25/16 10:39 AM (7 years, 4 months ago)

I think i may save the suspect one for when i start agar or the next run of jars and try the PSEA on this first set of homemade jars.  I dumped out one of the jars (outside) before to see what it was like. Wasnt really damp,but it did feel like a damn hockey puck it was so condensed. Do you or can you recommend any sponsor vendors you've had good luck with?


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Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23676350 - 09/25/16 10:48 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23676724 - 09/25/16 01:02 PM (7 years, 4 months ago)

Just got done inoculating 5 homemade brf jars with <5ml ms syringe total.  The jars were made last night following the pf tek ratio of 2:1:1 and pressure cooked at 15psi for 75 mins. They were left in the pc overnight to cool and inoculated today using my sab. How do they look?


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Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23677014 - 09/25/16 02:56 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23677207 - 09/25/16 04:09 PM (7 years, 4 months ago)

Yeah buddy,to the shelf they went. No foil to block the ge and right around 72F. I also was looking at a brf agar tek and have a ton of brf so i made 4 little 4oz jars with a tyvek and poly filter like the tek called for.  I'm throwing those in the pot to get some practice with agar during the wait :smile:


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23677272 - 09/25/16 04:33 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23677358 - 09/25/16 05:02 PM (7 years, 4 months ago)

On spores and agar

Doing this dramatically helps your chances as well as softer agar

Quote:

Trusted Cultivator said:
Quote:

Trusted Cultivator said:
Quote:

bodhisatta said:
Quote:

bodhisatta said:


take your spores to an inoculation loop. if you have a print then flame the loop dip in agar, pick spores off print and then swab the dish

if you have a syringe flame the loop and drop a few drops onto the loop to cool it down and then one more drop to load it with spores and then swab that








Quote:

bodhisatta said:
Try using an inoculation loop. Flame the syringe needle and the loop then squirt onto the loop to cool the needle and the loop. Squirt another drop to prime the loop. Then swab the dish.



Then you can see growth immediately, see contamination easier, grab healthy mycelium easier, and never worry about spore drops rolling all over a dish giving you germination everywhere at the same time.










also the agar link in my signature







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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: bodhisatta]
    #23677440 - 09/25/16 05:34 PM (7 years, 4 months ago)

Thank you! How do you feel about the agar substitute by doc34 https://www.shroomery.org/8514/Agar
I made 4 of the jars up because of the easy access to material and no extra cost. I have been reading over stro's clean up and isolation, and also your material. The agar substitute seemed pretty noob friendly and a good way to introduce myself to working with agar. This wouldn't be a long term answer, but for the short term until my funds for this replenish would it be a suitable substitute?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


Edited by Shineonyoucrazy (09/25/16 06:01 PM)


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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23677541 - 09/25/16 06:10 PM (7 years, 4 months ago)

Yes I would search for brf paste on the forum too and see what's been done


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: bodhisatta]
    #23677563 - 09/25/16 06:21 PM (7 years, 4 months ago)

Wonderful! Thank you for pointing me in the right direction. I'm excited to see how my homemade brf jars go, along with trying some agar work.  This place and you guys are awesome! Time to hit up more reading, but I'll update on both my projects.


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Keep your feet on the ground and your head in the clouds :mushroom2:


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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23677594 - 09/25/16 06:33 PM (7 years, 4 months ago)



Edited by bodhisatta (09/25/16 06:35 PM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: bodhisatta]
    #23680181 - 09/26/16 03:12 PM (7 years, 4 months ago)

Had one quick question in regards to the brf agar plates i made or just agar work in general.  I shot one of the jars last night with one drop because the bfr no pour agar tek i followed included making jars with a tyvek and poly fill filter otherwise i would have tried the smear method you suggested. i can already see all the little white spore droplets formed overnight, but i was wondering should i wait for 100% colonization to use a loop to transfer to another plate, or just when a good amount has occured? I read somewhere that since it was a ms syringe that i should transfer a sample before 100% to avoid alot of the other contamination that is present with a ms syringe, and then colonize another jar, and if i want start to isolate the best growth from there.  Any help you can lend would be appreciated! Just trying to figure the next best step while i have a couple days between.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23680229 - 09/26/16 03:23 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23680297 - 09/26/16 03:45 PM (7 years, 4 months ago)

Sweet! Just wanted to make sure i was on the right track with what i was reading. Since this will be a first transfer, I'll be at it sooner than later. Good luck with yours! What you working on? Isolation or just expanding your cultures?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23680404 - 09/26/16 04:33 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:26 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23680482 - 09/26/16 05:06 PM (7 years, 4 months ago)

Once i get a few more bucks I'd like to masked a bunch of pastyplates to play around with and use what i can isolate to inoculate some grains rather than using ms syringes.  I can see how this can exponentially explode like that quote says quite easily!


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23680550 - 09/26/16 05:25 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:26 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23680624 - 09/26/16 05:56 PM (7 years, 4 months ago)

Have you read how long you can keep an active culture on a plate and it still be viable? I think from what i understand a few months as long as you refrigerate to inhibit growth? What are you going to use for your grain? I'm hoping all goes well with the brf jars of mine.  Interestingly enough the non contaminated jars might actually work.  I see a few spots in some of the jars that are smaller than a pea, but bright white right where it was inoculated.  I'm just going to let them go and hope for the best. It's way early so I'll let them go for another week or two before they get the ax or not.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23680697 - 09/26/16 06:19 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:26 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23680872 - 09/26/16 07:02 PM (7 years, 4 months ago)

I'm also researching using a culture from agar to make a lc after i can obtain a clean isolate. Maybe that would be better for longer term storage than just on the plate.  I don't think I'll have to worry about it anytime soon


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Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23681049 - 09/26/16 07:46 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:26 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23681145 - 09/26/16 08:17 PM (7 years, 4 months ago)

Definatly the way to go, but i got take some baby steps lol. Sometimes i get way into big picture thinking, but right now i have to focus on what's in front of my face :grin: are you using the easy no pour pastyplates for your agar medium?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23681153 - 09/26/16 08:20 PM (7 years, 4 months ago)

Last link in signature has all the good info on here compiled


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23681195 - 09/26/16 08:30 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:26 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23681228 - 09/26/16 08:40 PM (7 years, 4 months ago)

Bodhisatta just put that in my favorites folder! Awesome to have it all right there thank you very much.  I just checked my account and i think i might have to go shopping for pastyplate materials and order half pint wide mouth jars.  I looked at every grocery store,craft store,hardware store,pretty much any store in the joining 3 counties and either they didn't carry them or they were out. Must be a lot of salsa making going on around my area:crazy2:


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23681253 - 09/26/16 08:46 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:27 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23681264 - 09/26/16 08:49 PM (7 years, 4 months ago)

Half pint wide mouth are hard to find. The regular mouth are far more common. Maybe skip the pf tek altogether


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Invisibleamidogen
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Re: Best way to go forward from my mw kit buying mistake. [Re: bodhisatta]
    #23681283 - 09/26/16 08:55 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:27 AM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: bodhisatta]
    #23681294 - 09/26/16 08:59 PM (7 years, 4 months ago)

I just got 24 for $25 shipped. I can live with that.  I definatly wanna work with some pf jars for a bit to dial things in,and also small scale projects until i get some good agar work under my belt to use with grains. That's more than enough for small pf ventures.  I really want to learn to be versatile and try different teks.
Large scale production isn't a huge concern as much as the learning experience and hobby of it. Although I won't get mad if i get some of the bulk yields i see some of you guys cranking out lol


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


Edited by Shineonyoucrazy (09/26/16 09:07 PM)


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OfflineShineonyoucrazy
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23688465 - 09/28/16 09:06 PM (7 years, 4 months ago)

*Update on the shitty jars, new homemade jars and first set of pastyplates...

I'm actually starting to see small (under the size of a pea) nice white spots right at the inoculation points.  I'm hoping they are mycelium cause there are no indications of any other growth (crazy contaminated mold or bacterial growth like in the other jars). This leads me to believe the other jars that were inoculated in the sab at the same time, with the same technique, just a different syringe may have been due to the syringe. My new homemade cakes are already showing  spots of bright white at the inoculation point a well, but after only a few days.  Fingers crossed they colonize with no contams. Then there is the pastyplates.... they came out awesome! Thank you guys for the guidence and reading. I just made a few plates with PSEA, so hopefully my agar project goes well.  I'd like to isolate some strong growth and make some plates to get a grain project going.  Thank you guys! Kits fucking blow!!!! This is way more interesting  :trippinbawelz:


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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