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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
#23677358 - 09/25/16 05:02 PM (7 years, 4 months ago) |
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On spores and agar
Doing this dramatically helps your chances as well as softer agar
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Trusted Cultivator said:
Quote:
Trusted Cultivator said:
Quote:
bodhisatta said:
Quote:
bodhisatta said:
take your spores to an inoculation loop. if you have a print then flame the loop dip in agar, pick spores off print and then swab the dish
if you have a syringe flame the loop and drop a few drops onto the loop to cool it down and then one more drop to load it with spores and then swab that


Quote:
bodhisatta said: Try using an inoculation loop. Flame the syringe needle and the loop then squirt onto the loop to cool the needle and the loop. Squirt another drop to prime the loop. Then swab the dish.

 
Then you can see growth immediately, see contamination easier, grab healthy mycelium easier, and never worry about spore drops rolling all over a dish giving you germination everywhere at the same time.
also the agar link in my signature
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Shineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: bodhisatta]
#23677440 - 09/25/16 05:34 PM (7 years, 4 months ago) |
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Thank you! How do you feel about the agar substitute by doc34 https://www.shroomery.org/8514/Agar I made 4 of the jars up because of the easy access to material and no extra cost. I have been reading over stro's clean up and isolation, and also your material. The agar substitute seemed pretty noob friendly and a good way to introduce myself to working with agar. This wouldn't be a long term answer, but for the short term until my funds for this replenish would it be a suitable substitute?
-------------------- Keep your feet on the ground and your head in the clouds
Edited by Shineonyoucrazy (09/25/16 06:01 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23677541 - 09/25/16 06:10 PM (7 years, 4 months ago) |
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Yes I would search for brf paste on the forum too and see what's been done
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Shineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: bodhisatta]
#23677563 - 09/25/16 06:21 PM (7 years, 4 months ago) |
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Wonderful! Thank you for pointing me in the right direction. I'm excited to see how my homemade brf jars go, along with trying some agar work. This place and you guys are awesome! Time to hit up more reading, but I'll update on both my projects.
-------------------- Keep your feet on the ground and your head in the clouds
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23677594 - 09/25/16 06:33 PM (7 years, 4 months ago) |
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Edited by bodhisatta (09/25/16 06:35 PM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: bodhisatta]
#23680181 - 09/26/16 03:12 PM (7 years, 4 months ago) |
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Had one quick question in regards to the brf agar plates i made or just agar work in general. I shot one of the jars last night with one drop because the bfr no pour agar tek i followed included making jars with a tyvek and poly fill filter otherwise i would have tried the smear method you suggested. i can already see all the little white spore droplets formed overnight, but i was wondering should i wait for 100% colonization to use a loop to transfer to another plate, or just when a good amount has occured? I read somewhere that since it was a ms syringe that i should transfer a sample before 100% to avoid alot of the other contamination that is present with a ms syringe, and then colonize another jar, and if i want start to isolate the best growth from there. Any help you can lend would be appreciated! Just trying to figure the next best step while i have a couple days between.
-------------------- Keep your feet on the ground and your head in the clouds
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23680229 - 09/26/16 03:23 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:25 AM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
#23680297 - 09/26/16 03:45 PM (7 years, 4 months ago) |
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Sweet! Just wanted to make sure i was on the right track with what i was reading. Since this will be a first transfer, I'll be at it sooner than later. Good luck with yours! What you working on? Isolation or just expanding your cultures?
-------------------- Keep your feet on the ground and your head in the clouds
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23680404 - 09/26/16 04:33 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:26 AM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
#23680482 - 09/26/16 05:06 PM (7 years, 4 months ago) |
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Once i get a few more bucks I'd like to masked a bunch of pastyplates to play around with and use what i can isolate to inoculate some grains rather than using ms syringes. I can see how this can exponentially explode like that quote says quite easily!
-------------------- Keep your feet on the ground and your head in the clouds
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23680550 - 09/26/16 05:25 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:26 AM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
#23680624 - 09/26/16 05:56 PM (7 years, 4 months ago) |
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Have you read how long you can keep an active culture on a plate and it still be viable? I think from what i understand a few months as long as you refrigerate to inhibit growth? What are you going to use for your grain? I'm hoping all goes well with the brf jars of mine. Interestingly enough the non contaminated jars might actually work. I see a few spots in some of the jars that are smaller than a pea, but bright white right where it was inoculated. I'm just going to let them go and hope for the best. It's way early so I'll let them go for another week or two before they get the ax or not.
-------------------- Keep your feet on the ground and your head in the clouds
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23680697 - 09/26/16 06:19 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:26 AM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
#23680872 - 09/26/16 07:02 PM (7 years, 4 months ago) |
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I'm also researching using a culture from agar to make a lc after i can obtain a clean isolate. Maybe that would be better for longer term storage than just on the plate. I don't think I'll have to worry about it anytime soon
-------------------- Keep your feet on the ground and your head in the clouds
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23681049 - 09/26/16 07:46 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:26 AM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
#23681145 - 09/26/16 08:17 PM (7 years, 4 months ago) |
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Definatly the way to go, but i got take some baby steps lol. Sometimes i get way into big picture thinking, but right now i have to focus on what's in front of my face are you using the easy no pour pastyplates for your agar medium?
-------------------- Keep your feet on the ground and your head in the clouds
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23681153 - 09/26/16 08:20 PM (7 years, 4 months ago) |
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Last link in signature has all the good info on here compiled
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23681195 - 09/26/16 08:30 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:26 AM)
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Shineonyoucrazy
Apprentice fungi


Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
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Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
#23681228 - 09/26/16 08:40 PM (7 years, 4 months ago) |
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Bodhisatta just put that in my favorites folder! Awesome to have it all right there thank you very much. I just checked my account and i think i might have to go shopping for pastyplate materials and order half pint wide mouth jars. I looked at every grocery store,craft store,hardware store,pretty much any store in the joining 3 counties and either they didn't carry them or they were out. Must be a lot of salsa making going on around my area
-------------------- Keep your feet on the ground and your head in the clouds
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
#23681253 - 09/26/16 08:46 PM (7 years, 4 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:27 AM)
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