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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23674723 - 09/24/16 05:44 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:24 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
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Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23675249 - 09/24/16 09:00 PM (7 years, 4 months ago)

Just put together my first 5 jars! They're in the pc right now. Man i really wish i would have done this from the start. I could make jars for years for how much i dropped on the bs kit. They look really nice,fluffy,and a nice 1/2 inch dry vermiculite layer on top. Can't wait till tomorrow to give them a go.  I took your advice, and started small so i have plenty ms to go around for the coming tries and also some agar work in the near future. I popped a premade jar open to see what it was like, seemed like a rock.  I don't have high hopes for the kit jars, but making my own gives me some hope. Could that be why I'm not seeing growth on the other non contam jars.  Too tightly packed?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineKenetic
Nam Sayin
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23675313 - 09/24/16 09:36 PM (7 years, 4 months ago)

Quote:

Shineonyoucrazy said:
Here are some suspect jars




Normally innocent until proven guilty, but those jars are well beyond suspect.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
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Re: Best way to go forward from my mw kit buying mistake. [Re: Kenetic]
    #23675336 - 09/24/16 09:44 PM (7 years, 4 months ago)

The suspect jars were from a mw growkit. I went out and got a pressure cooker today and other supplies to make my own following the pf tek 2:1:1 ratio for making brf cakes.  The ones from the kit had at least an inch dry verm layer and super condensed.  Could that be inhibiting the ones that aren't showing growth?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineKenetic
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23675359 - 09/24/16 09:59 PM (7 years, 4 months ago)

I started with pf tek and learned that you shouldn't pack the sub too dense because it doesn't allow for air pockets to penetrate the sub.  During shipping your jars probably got messed up and became too dense or something. 
One inch of verm on top isn't going to cause a problem, but a "super condensed" sub will.  After you mix the ingredients, loosely put them in the jar and apply the verm layer on top.  inoculate and put on a shelf until they're done.  Don't mess with them too much

I'd just follow the pf tek as it is, but keep in mind you don't want to compact a pf cake.

edit:  changed their to they're


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Edited by Kenetic (09/24/16 10:06 PM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: Kenetic]
    #23675393 - 09/24/16 10:12 PM (7 years, 4 months ago)

Awesome! That makes me feel good about the jars i just made myself then. The radio seemed nice (squeezed the vermiculite and water mixture and just a few drops came out like the tek said, scooped the brf mixture into the jars making sure not to pack them, and gave it a nice 1/2 dry layer, then they went into the pc. They are cooling now in the pc after a little more than 60 mins in @15psi, and I'll try inoculating them tomorrow morning when they cool. All in all i have better hopes for my homemade jars.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineKenetic
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23675398 - 09/24/16 10:15 PM (7 years, 4 months ago)

You are on your way to success.  If you fail, try again.  and again if you must.  You'll get it right soon with that attitude.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
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Re: Best way to go forward from my mw kit buying mistake. [Re: Kenetic]
    #23675417 - 09/24/16 10:25 PM (7 years, 4 months ago)

Thank you! Definatly bound and determined to get it right, so it's only a matter of patients,time,practice, and of course continued reading and research.  I'm definatly ok with all of that though. I needed a fun rewarding hobby, and even though it's very early this seems like this is on its way to becoming just that. This is a wonderful thing,and hopefully someday I'll be knowledgeable enough to pass the torch like so many of you guys are. Before this i worked on mimosa and acacia extractions, so this is a fun jump.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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OfflineKenetic
Nam Sayin
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Registered: 08/24/14
Posts: 4,389
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Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23675464 - 09/24/16 10:57 PM (7 years, 4 months ago)

Nice!  Hobbies are cool.  I have so many I can't master any of them.  Or afford them, or make time for them.  Luckily mushies don't take up a lot of my time (unless I'm on a forum) so it it definitely rewarding.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23676053 - 09/25/16 08:17 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:24 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23676166 - 09/25/16 09:31 AM (7 years, 4 months ago)

Thanks amidogen! I'm lucky to have a pretty full schedule outside of this that keeps me busy in the time between.  I picked up the pc yesterday and supplies and made my first run of jars.  I figure is would be a great investment that could open a lot of avenues here to branch out.  I like the idea you have to spreading your time around/not putting your eggs all in one basket.  Next check i want to get the supplies for the no pour agar tek and try working with the ms in that to clean and isolate.  I have a question. Out of the 18 jars i inoculated 9 are definatly contaminated at the point of inoculation.  Those were inoculated with one spore stringe and all the non contaminated jars with another. They both came from site vendors, but the contaminated jar syringe came from a vendor i didn't tell i was a member from here.  Do you think it was the syringe? All jars were inoculated in a sab using sterile procedures, flaming the needle between jars, etc. I just find it odd only those jars (all) have contamination using the exact procedure at the same time. Thoughts?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23676178 - 09/25/16 09:41 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:24 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23676283 - 09/25/16 10:21 AM (7 years, 4 months ago)

*Yes two different syringes from two different vendors

*Yes they are both shroomery vendors from the sponsor list

*The contaminated jars came from a syringe i did not tell the vendor i was a member

*The non contaminated jars came from a syringe i told the vendor i was a member (also got a freebie for being a member)

In total i have 2 left from the non contaminated jar vendor (PE and PSEA) and 1 from the contaminated jar vendor left (golden teacher)

I'm just up in the air about using the second syringe from the possibly bad set. In all fairness the jars weren't mine and i kept them in a tub in the closet at 76F (no heater just the tub kept that temp in the closet) with the foil lightly over the top until i learned that was wrong. Do you think the lack of proper ge/temps did it? I just find it odd that it's only those jars, but the ms from one could of been cleaner than the other.  I've read no ms is 100% clean which is why people usually go agar to clean it up first for teks other than brf.

Also the foil and temp combo could be why the ones not showing contamination, but also no growth could be slow right? When we talked a couple days ago and i said i fixed the temp, i took them all out and foil off the top. I hope that kicks the growth into gear.


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23676298 - 09/25/16 10:29 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23676322 - 09/25/16 10:39 AM (7 years, 4 months ago)

I think i may save the suspect one for when i start agar or the next run of jars and try the PSEA on this first set of homemade jars.  I dumped out one of the jars (outside) before to see what it was like. Wasnt really damp,but it did feel like a damn hockey puck it was so condensed. Do you or can you recommend any sponsor vendors you've had good luck with?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23676350 - 09/25/16 10:48 AM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23676724 - 09/25/16 01:02 PM (7 years, 4 months ago)

Just got done inoculating 5 homemade brf jars with <5ml ms syringe total.  The jars were made last night following the pf tek ratio of 2:1:1 and pressure cooked at 15psi for 75 mins. They were left in the pc overnight to cool and inoculated today using my sab. How do they look?


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23677014 - 09/25/16 02:56 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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OfflineShineonyoucrazy
Apprentice fungi

Registered: 09/22/16
Posts: 917
Loc: Somewhere over the rainbo...
Last seen: 4 years, 5 months
Re: Best way to go forward from my mw kit buying mistake. [Re: amidogen]
    #23677207 - 09/25/16 04:09 PM (7 years, 4 months ago)

Yeah buddy,to the shelf they went. No foil to block the ge and right around 72F. I also was looking at a brf agar tek and have a ton of brf so i made 4 little 4oz jars with a tyvek and poly filter like the tek called for.  I'm throwing those in the pot to get some practice with agar during the wait :smile:


--------------------
Keep your feet on the ground and your head in the clouds :mushroom2:


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Best way to go forward from my mw kit buying mistake. [Re: Shineonyoucrazy]
    #23677272 - 09/25/16 04:33 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:25 AM)


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