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Mushroom-Hut Shop: Mono Tub Substrate, Substrate Bags, Substrate Mix

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InvisiblebodhisattaMDiscordReddit
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Re: A question about selective media (moved) [Re: ballsalsa]
    #23692016 - 09/29/16 08:15 PM (7 years, 3 months ago)

I wouldn't be surprised if cubensis tolerated higher alcohol than yeast anyway. I've seen mold growing in 40% 80proof liquor bottles. Not badly but its grown


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InvisibleballsalsaMDiscord
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Re: A question about selective media (moved) [Re: bodhisatta]
    #23692110 - 09/29/16 08:35 PM (7 years, 3 months ago)

really?  eating residual sugars maybe.  or using the alcohol as a carbon source even?
thats disheartening though in a sense because that means one could accidentally select for resistant contams. :facepalm: i didn't think about resistant molds.


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Re: A question about selective media (moved) [Re: ballsalsa]
    #23692132 - 09/29/16 08:39 PM (7 years, 3 months ago)

Shit. . . Who here hasn't misted their sub with alcohol at least once. . .

:guiltyascharged:


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InvisiblebodhisattaMDiscordReddit
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Re: A question about selective media (moved) [Re: ballsalsa]
    #23692240 - 09/29/16 09:08 PM (7 years, 3 months ago)

Quote:

ballsalsa said:
really?  eating residual sugars maybe.  or using the alcohol as a carbon source even?
thats disheartening though in a sense because that means one could accidentally select for resistant contams. :facepalm: i didn't think about resistant molds.



Only in brandy have I seen it. Also with fruit flies in the bottle


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Re: A question about selective media (moved) [Re: bodhisatta]
    #23692314 - 09/29/16 09:31 PM (7 years, 3 months ago)

oh, ok, then the mold might have been eating dead flies or castoff pupae casings or some such.  Drosophila melanogaster (a common lab species of fruit fly) select strongly for alcohol tolerance and are commonly found in wine cellars, much more so than other Drosophila spp.


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Re: A question about selective media (moved) [Re: Pastywhyte]
    #23692674 - 09/29/16 11:38 PM (7 years, 3 months ago)

Quote:

Pastywhyte said:
Shit. . . Who here hasn't misted their sub with alcohol at least once. . .

:guiltyascharged:




You have gained 1,000 Morty points for your comment.

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Re: A question about selective media (moved) [Re: bodhisatta]
    #23692819 - 09/30/16 01:13 AM (7 years, 3 months ago)

was the mold growing on the surface of the brandy, or up above in or around the neck of the bottle?


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Re: A question about selective media (moved) [Re: ballsalsa]
    #23694633 - 09/30/16 05:04 PM (7 years, 3 months ago)

Quote:

ballsalsa said:
oh, ok, then the mold might have been eating dead flies or castoff pupae casings or some such.  Drosophila melanogaster (a common lab species of fruit fly) select strongly for alcohol tolerance and are commonly found in wine cellars, much more so than other Drosophila spp.



it doesn't matter what it was eating.. what matters that it was growing in 40% ethanol

it was growing IN the brandy, not on the cap. pour spouts (hence how flies got in)

small sphere colonies probably a good 10-20 of them all about 0.5-1cm in diameter. floating in the liquid not on the surface


Edited by bodhisatta (09/30/16 05:10 PM)


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Re: A question about selective media (moved) [Re: bodhisatta]
    #23695232 - 09/30/16 08:00 PM (7 years, 3 months ago)

damn


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