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Invisibleamidogen
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Registered: 05/07/16
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Re: Mycelium growth in two hours [Re: Trocity]
    #23671469 - 09/23/16 03:46 PM (7 years, 4 months ago)

.


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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:27 AM)


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OfflineMadSeasonStudent
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Re: Mycelium growth in two hours [Re: leech911]
    #23671574 - 09/23/16 04:16 PM (7 years, 4 months ago)

Actually you can see new growth in that time frame of 2 hours


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Invisibleamidogen
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Re: Mycelium growth in two hours [Re: MadSeasonStudent]
    #23671591 - 09/23/16 04:23 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:27 AM)


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OfflineTrocity
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Re: Mycelium growth in two hours [Re: amidogen]
    #23671663 - 09/23/16 04:58 PM (7 years, 4 months ago)

Appreciate the input very much. I will keep updated


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Offlinetombosley8
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Re: Mycelium growth in two hours [Re: Trocity]
    #23671684 - 09/23/16 05:11 PM (7 years, 4 months ago)

how long were the jars sitting after sterilization?

The tin foil may have created too much moisture on your "dry" verm layer causing a failure to filter molds spores.

either that or maybe insufficient sterilization?

thought mold would be easily sterilized if you heated them for an hour in even close to boiling water :shrug:

maybe you tilted the jar and disturbed the verm barrier?

Just can't believe something could really germinate and grow that much in two hours.


I was almost thinking somehow you noc'd up a jar that was noc'd up days/weeks before by mistake and didn't notice until after you noc'd it up again.

But then when you said it was mold I was sure there was more to the story


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Edited by tombosley8 (09/23/16 05:12 PM)


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OfflineTrocity
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Re: Mycelium growth in two hours [Re: tombosley8]
    #23671689 - 09/23/16 05:14 PM (7 years, 4 months ago)

Guys. I got my spores the day I innoced em. Lol <3


Edited by Trocity (09/23/16 05:15 PM)


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Offlinetombosley8
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Re: Mycelium growth in two hours [Re: Trocity]
    #23671724 - 09/23/16 05:31 PM (7 years, 4 months ago)

Quote:

Trocity said:
Guys. I got my spores the day I innoced em. Lol <3



:facepalm:
ok edited so you don't get confused(please answer my questions):wink::
Quote:

tombosley8 said:
how long were the jars sitting after sterilization?

The tin foil may have created too much moisture on your "dry" verm layer causing a failure to filter molds spores.

either that or maybe insufficient sterilization?

thought mold would be easily sterilized if you heated them for an hour in even close to boiling water :shrug:

maybe you tilted the jar and disturbed the verm barrier?

Just can't believe something could really germinate and grow that much in two hours.





there nothing about your spores


Edited by tombosley8 (09/23/16 05:33 PM)


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OfflineTrocity
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Re: Mycelium growth in two hours [Re: tombosley8]
    #23672102 - 09/23/16 08:01 PM (7 years, 4 months ago)

Jars sat for a week. Sterilized in boiling water for a little over an hour. And I tried specifically not to tilt why inoculating but it's possible.


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OfflineMushierage
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Re: Mycelium growth in two hours [Re: Trocity]
    #23672148 - 09/23/16 08:19 PM (7 years, 4 months ago)

Ok, right there.  You said your jars were sitting for a week before inoculation. 

This will give contaminants every ample opportunity to get at your substrate.  I'm not saying one did, but a week of just sitting there without inoculation is a week that mold spores have a chance to germinate. 

So while you think you have a couple hour old spot of mycelium, what it more probably is, is a freshly germinated mold, from spores that had a week head start.


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Invisibleamidogen
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Re: Mycelium growth in two hours [Re: Trocity]
    #23672188 - 09/23/16 08:34 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:28 AM)


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InvisibleInocuole
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Re: Mycelium growth in two hours [Re: amidogen]
    #23672225 - 09/23/16 08:48 PM (7 years, 4 months ago)

Just leaving stuff that was properly sterilized for a week shouldn't cause anything to contam.  It's normal for spores to take up to 2-3 weeks to germinate.  It's not normal for contams to come up in that period.  There was a fuck up, and letting them sit a week, while bad, wasn't the vector.  That mold would've shown up anyway even if he inoculated immediately.  It's mold, for fucks sake.

Still, this does make the fact that you thought it was cubensis mycelium all the more ridiculous.


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Offlinetombosley8
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Re: Mycelium growth in two hours [Re: tombosley8]
    #23672233 - 09/23/16 08:48 PM (7 years, 4 months ago)

I've had many many brf cakes and grain jars for that matter sit for way over a week unninoculated many times and never had a problem...

The problem here is filter failure.

one of my biggest problems with the pf tek(besides being a PITA to fruit) is the dry verm barrier for the filter.

I can see how newbs may disturb easily the verm barrier with any accidental tipping say when moving the pc or when placing into and out of sab or constantly looking for growth.
I was thinking you may have accidently tilted this jar before inoculating casing the verm barrier to fail as a filter.

Moreso I don't understand why so many newbs say they read to keep the foil on...

BAD IDEA>>>



What I believe truly happened here is like I said before
Quote:

tombosley8 said:

The tin foil may have created too much moisture on your "dry" verm layer causing a failure to filter molds spores.






So never keep the foil on the jars for too long as I have seen similar happen IME

Also you should get into agar and bulk. Cakes are a huge waste IMO.


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Edited by tombosley8 (09/23/16 08:51 PM)


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OfflineTrocity
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Re: Mycelium growth in two hours [Re: tombosley8]
    #23672511 - 09/23/16 10:36 PM (7 years, 4 months ago)

Okay, I sterilized between each jar. Think I have a good chance with the other jars? And you guys are making fun of me for being a newb and its fine fire away.


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InvisibleMad Season
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Re: Mycelium growth in two hours [Re: Trocity]
    #23672528 - 09/23/16 10:41 PM (7 years, 4 months ago)

Honestly, the fact it germinated in the middle of the jar makes me think this is a syringe issue, hell could have just ridden the syringe tip after you did a hole or 2 prior. I do agree that leaving a jar uninoculated for a week wasn't the issue. I do that all the time lol. If it was a filter issue, it would be closer to the top of the jar IMO/IME. This looks more like an inoc induced contam.


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InvisibleBoogieman47
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Re: Mycelium growth in two hours [Re: Trocity]
    #23672535 - 09/23/16 10:42 PM (7 years, 4 months ago)

Ya dont take offense its all outta love as long as youre not a douche about it .... i think if the other jars havent showed anything yet they have a chance but dont get bummed if they go bad start reading about agar while you wait and give it a shot that way you can guaruntee you have clean myc


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Offlinetombosley8
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Re: Mycelium growth in two hours [Re: Boogieman47]
    #23672556 - 09/23/16 10:51 PM (7 years, 4 months ago)

2 hours for germination and that much growth, huh?

ok I'll take your word for it but definitely something new to me.

I was thinking it had grown from the top and had just peeked through to the side by the time op saw it.

OP, was there any sign of growth on the top under the verm layer when you opened and looked?

or was it all just in that one spot in the middle as it appears in your picture?


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InvisibleMad Season
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Re: Mycelium growth in two hours [Re: tombosley8]
    #23672566 - 09/23/16 10:54 PM (7 years, 4 months ago)

Yeah I haven't seen any molds germinate in 2 hours as well... I have seen yeast and bacteria attack within a few hours though. It keeps happening with my wild hericium I found. Fuckin annoying. Yeast is the hardest shit I've ever had to cleanup.


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OfflineTrocity
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Re: Mycelium growth in two hours [Re: Mad Season]
    #23672822 - 09/24/16 01:22 AM (7 years, 4 months ago)

Tom Bosley just that one spot. And I hope it's not a syringe issue... I have faith the other ones will be fine. Fingers crossed


Edited by Trocity (09/24/16 12:37 PM)


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InvisibleInocuole
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Re: Mycelium growth in two hours [Re: Trocity]
    #23672830 - 09/24/16 01:28 AM (7 years, 4 months ago)

Read the rules and consider editing your post.

http://www.shroomery.org/forums/showflat.php/Number/14960167


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OfflineTrocity
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Re: Mycelium growth in two hours [Re: Trocity]
    #23672833 - 09/24/16 01:29 AM (7 years, 4 months ago)

I don't see how it would be a syringe issue if the mold came up somewhere I didn't have the needle


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