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aghabheegy
Stranger
Registered: 09/22/16
Posts: 14
Last seen: 2 months, 2 hours
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Bulk substrate colonization - seems to be taking way too long
#23669224 - 09/22/16 07:45 PM (7 years, 4 months ago) |
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Started out with 5lbs of rye berries, fully colonized.
Spawned to two different tubs of hpoo, 10lbs each - so about 1:4 ration.
Hpoo was aged, no smell... in face, very aged... most of it was almost soil-like.
1 tub was not pasteurized, simply added a bit of water and mixed in 2.5 lbs of grain, drilled 5 tiny holes (1/16") into lid.
1 tub was pasteurized, a bit more moist as a result, mixed in 2.5lbs, drilled 5 tiny holes (1/16") into lid.
Placed both in closet and let sit... closet is temp controlled to 75 degrees.
It has been 11 days, and while the mycelium has grown around the grain, it doesn't appear to be colonizing the substrate...
1st pic is pasteurized, 2nd is not. You can see the mycelium is yellowing (waste products?)... so it doesn't appear that it's done growing... just concerned with how long it's taking...
Should I just be patient?

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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Bulk substrate colonization - seems to be taking way too long [Re: aghabheegy]
#23669244 - 09/22/16 07:51 PM (7 years, 4 months ago) |
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Why wouldnt you pasteurize your poo man? That shits going to go bad soon more then likely
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aghabheegy
Stranger
Registered: 09/22/16
Posts: 14
Last seen: 2 months, 2 hours
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Re: Bulk substrate colonization - seems to be taking way too long [Re: Boogieman47]
#23669253 - 09/22/16 07:55 PM (7 years, 4 months ago) |
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Just running a test... i've had success without pasteurization before, wanted to see the difference... might be regretting it soon...
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Bulk substrate colonization - seems to be taking way too long [Re: Boogieman47] 1
#23669257 - 09/22/16 07:56 PM (7 years, 4 months ago) |
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if you don't pasteurize it it's already bad. you should toss it out and start over without glaring mistakes. there's resources in my signature for how to make bulk substrates that grow mushrooms. save experimentation for after you have some experience and can change a single variable at a time with a proven culture
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Bulk substrate colonization - seems to be taking way too long [Re: bodhisatta]
#23669265 - 09/22/16 07:58 PM (7 years, 4 months ago) |
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Bodhi knows man and you should check out those links just for more knowledge
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Bulk substrate colonization - seems to be taking way too long [Re: aghabheegy]
#23669274 - 09/22/16 08:02 PM (7 years, 4 months ago) |
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Quote:
aghabheegy said: Just running a test... i've had success without pasteurization before, wanted to see the difference... might be regretting it soon...
Anybody could've told you the results of this test without you having to waste materials..
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Bulk substrate colonization - seems to be taking way too long [Re: Inocuole]
#23669346 - 09/22/16 08:26 PM (7 years, 4 months ago) |
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hello OP, welcome to the forum!
bodhi's advice is spot-on. it makes it much easier to debug and figure out what went wrong when there are less glaring problems, might as well set yourself up for success
also, since the density of substrates is going to vary so greatly and with water content, we usually discuss spawn and substrate measurements in terms of volume rather than weight, and use field capacity as a reference for water content (err on the dry side)
you have had success without pasteurization before? with coir or with manure? id love to hear more about the specific context you are referring to, out of curiosity 
even if you won the mycology lottery and managed to get some fruits off of unpasteurized bulk substrate in the past, it is certainly not something you could count on
in any case, you are in the right place to learn cubensis culture to your heart's content
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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aghabheegy
Stranger
Registered: 09/22/16
Posts: 14
Last seen: 2 months, 2 hours
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Re: Bulk substrate colonization - seems to be taking way too long [Re: c10h12n2o]
#23669483 - 09/22/16 09:16 PM (7 years, 4 months ago) |
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Thanks for all the responses, i will definitely check out the posts...
What about the pasteurized batch? Any advice on that? I definitely had that close to field capacity (only a drop or two when squeezing a handful)... Is it normal to not have it fully colonized at close to two weeks after introduction of colonized grain?
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Bulk substrate colonization - seems to be taking way too long [Re: aghabheegy]
#23669942 - 09/23/16 01:43 AM (7 years, 4 months ago) |
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how did you pasteurize it, specifically?
its not terribly unusual for it to take that long, what race/variety are you working with? i give stuff up to a month before i decide to move onto faster projects (most take less than 10 days with a high spawn rate, so i dont wait too long unless its something i am particularly invested in
they usually colonize faster as the colonies become larger, and if they arent it often indicates contam problems, either now or down the road
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Bulk substrate colonization - seems to be taking way too long [Re: c10h12n2o]
#23670087 - 09/23/16 05:22 AM (7 years, 4 months ago) |
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Maybe, excluding contamed sub, your sub is just too dense? I alway mix hpoo with straw or verm at least to fluff it up a bit. And I have no idea what you were trying to accomplish by testing an unpasteurized poo sub.
11 days and that progress? Your sub is fucked.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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aghabheegy
Stranger
Registered: 09/22/16
Posts: 14
Last seen: 2 months, 2 hours
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Re: Bulk substrate colonization - seems to be taking way too long [Re: Kenetic]
#24186782 - 03/23/17 05:31 PM (6 years, 10 months ago) |
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So, that batch died and went to hell. I have since had some decent luck with bulk grows, but still learning.
I am having some weird issues with a new grow, will post in separate thread. Thanks again for all the advice.
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