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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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First PE: Tubs, Remove Blobs Before Casing?
#23668647 - 09/22/16 04:23 PM (7 years, 4 months ago) |
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good evening lovely people, i am a PE virgin and hope some of you hookers can talk me through it, im nervous... i just want my first time to be special, ya know?
lol... so i have been messing around with PE from spores on agar for around 2 months now, and its been frustratingly wimpy and slow to colonize. I have had a few decent rhizo sectors, but nothing consistent or terribly organized, hopefully they will stabilize soon
so ive made LIs and spawn at 3 different points in the series. The first tub colonized quickly except for two areas about the size of a quarter, which was extremely slow to colonize. 26 days after spawning, those same patches STILL arent colonized (probably bacterial?). i used 4 mycology quarts spawn on this tub, but on all subsequent tubs i have been using large spawn bags at 5+ quarts
Ive been ready to case this tub for over 2 weeks, but kept waiting for those spots to finish.
so the second one was spawned 13 days ago and looks great. its been like this a few days, but i am only now getting the opportunity to case & fruit it

my plan was to go ahead and case them both and hope for the best. First one looks pretty gnarly to me, but i have never grown PE before so i dont know how abnormal this is. looks like blobs with some contams to my inexperienced eye, idk.
I was also considering cloning that big blob, even though there are not really any desirable characteristics expressed. I suspect the conditions had a lot to do with the blob's formation, and hope that maybe i could isolate some aggressive strains (or at least a decently organized culture) after a few transfers. does this make any sense?
what do yall think? and if the answer is to case and fruit both, should i remove anything from that first tub before casing? and would it be safe to fruit it in my main fruiting room, or should i isolate it at the expense of optimal conditions?
thanks in advance
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: c10h12n2o]
#23668673 - 09/22/16 04:35 PM (7 years, 4 months ago) |
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Those blobs will turn out to be fatties leave them alone you can still put a light casing on that one very thin... the other one case normal 1/4" or so and fruit check out mycologist217's penis envy grow he had a ton of blobs on two tubs that turned out nice
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: Boogieman47]
#23668698 - 09/22/16 04:50 PM (7 years, 4 months ago) |
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will do, thanks buddy!!
do you think its worth going ahead and cloning that big blob? not to use it as a clone culture, but to further isolate it? anything rhizo or aggressive would be much nicer than the wimpy PE plates im working with 8 transfers into isolation from diluted spores
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Boogieman47
Let's boogie


Registered: 03/05/16
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: c10h12n2o]
#23668703 - 09/22/16 04:53 PM (7 years, 4 months ago) |
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Actually blobs can push out some killer fruits man as far as clones go just because it was a mutant doesnt mean it dont have good genetics in it... i always take atleast two clones per tub
Edited by Boogieman47 (09/22/16 04:54 PM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: Boogieman47]
#23668747 - 09/22/16 05:08 PM (7 years, 4 months ago) |
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thats what i was thinking, especially with further isolation, there could be a lot of variety in a blob
i will go ahead and clone that big one to some plates, and lightly case the rest of them, thanks for the advice 
if i can get a PE clone culture that looks like this after a few transfers i will be stoked 
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Boogieman47
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Registered: 03/05/16
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: c10h12n2o]
#23668764 - 09/22/16 05:14 PM (7 years, 4 months ago) |
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May even after the first id let that thing grow up but thats on you this was right from the clone
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: Boogieman47]
#23668789 - 09/22/16 05:21 PM (7 years, 4 months ago) |
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wow thats nice! what race is that? not a PE blob was it?
i have a bunch of much better looking PE tubs coming in behind it, i will be happy if i get anything at all out of that first one (including a decent culture), looks pretty gnarly to me.... in any case i'll gladly sacrifice a potentially giant fruit in tub #1 if it gets me closer to a more aggressive PE culture sooner
when i made that first one i was still using a pasteurization thermometer that i later learned was off by a good bit lol... things have certainly improved since i switched to waterproof probes
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Boogieman47
Let's boogie


Registered: 03/05/16
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: c10h12n2o]
#23668803 - 09/22/16 05:26 PM (7 years, 4 months ago) |
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https://www.shroomery.org/forums/showflat.php/Number/23374414/page/6 check this thread out.. that was a pe clone not a blob i think that one was a short stubby one from a cluster ...
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: Boogieman47]
#23703809 - 10/03/16 02:08 PM (7 years, 3 months ago) |
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thanks again for the advice buddy!! 
UPDATE:
ok so this has been fun. i cloned the first blob, the tub that had the blobs continued to put out some massive blobs. I harvested one that seemed ready (VERY heavy), and several more properly formed fruits have started to come in

there is a small cluster that looks great, which has been tempting me hard. I am considering cloning a pin from that cluster

the other tub has been colonizing the casing, no pins yet. tub with fruits never really colonized the casing (fruited both at casing). Strangely, first tub smells kinda funky but seems to be doing fine. Id read that PE likes a wetter than normal casing, and it seems to be true
probably about to harvest the rest of the blobs, since they seem to be getting a bit squishy
i finally have some really good looking PE cultures on agar, nice and rhizo/aggressive, where the cultures i used for these tubs were super wispy and slow. i really look forward to working with the clone cultures, isolates, and even just the more aggressive, rhizo, "limited" cultures i have now
any feedback/advice is much appreciated!!
warm regards
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Edmunter
Mr



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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: c10h12n2o]
#23773067 - 10/26/16 11:53 AM (7 years, 3 months ago) |
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So now you've done this grow would you advise doing a smaller grow first to get the genetics to have a ripping mono tub later?
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Boogieman47
Let's boogie


Registered: 03/05/16
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: Edmunter]
#23773080 - 10/26/16 11:57 AM (7 years, 3 months ago) |
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Too be honest you may have needed more fae to have had better results?? Im not sure i have had some shitty tubs of ms pe and then have had some really good ones so it could just of been the genetics i also have never had blobs on ms grows only on my clones...
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enlightenment
alchemist


Registered: 08/09/09
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: Edmunter]
#23773082 - 10/26/16 11:58 AM (7 years, 3 months ago) |
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Quote:
Edmunter said: So now you've done this grow would you advise doing a smaller grow first to get the genetics to have a ripping mono tub later?
That's what I always do.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: enlightenment]
#23773201 - 10/26/16 12:49 PM (7 years, 3 months ago) |
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yes, for sure, though for my purpose a mono was suited. the whole point of this tub was to take a bunch of spore swabs for the second round of my giveaway thread, and to get some fruits to clone so that i can work towards more aggressive cultures
this has been my first PE tub, did 2 at a time, the one pictured has the usual PE phenotype, but the other one was looked NOTHING like them, tall skinny with widely opened caps lol.... damn MS
the really crazy part is how much i have harvested off of the pictured tub!!! almost 2kg wet so far! it has fruited VERY unevenly, with fruits on it constantly since it first pinned :/
got a lot more weight off of it than i expected considering how unevenly it fruited. I have a few grain masters made from more limited MS cultures (further isolated) and some clone cultures, i would expect them to do much better
@noob47 i actually had TONS of FAE, like more than any grow ive ever done, i think it was just MS PE luck of the draw. At the exact same time in the same room i had a clone tub of AA+ that i was trying to provide the absolute max possible FAE/evap and water, and it produced my biggest yield and fruit yet (3498 grams and 296 grams respectively)
  
that was 1 flush from 1 tub! same fruiting room at the same time as that first PE tub i did
btw the blobs only showed up before i cased, after casing most of them turned into monsters
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: c10h12n2o]
#23773407 - 10/26/16 01:59 PM (7 years, 3 months ago) |
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Oh right on .. i think i have been having an issue with drying out due to the ac always blasting .. ya pe ms is really a crap shoot i hate it .. how many clones did you take ?
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: Boogieman47]
#23773440 - 10/26/16 02:08 PM (7 years, 3 months ago) |
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for those tubs i had a fan running on a timer, a vornado humidifier, and a heater that blows air, all in the same room lol.... had to mist every hour or so. it damn sure put some weight on that 1 tub though
i took dozens of clones lol... and never seen so much contamination!! some kind of bright red bacteria on almost every one of them. i bet it has something to do with the extra moist casing layer i had heard PE likes... im gonna take some more today and use gentamicin and chloramphenicol agar, since i was making some anyway for the last plate before some slants
i cant wait to have some better cultures to work with, these are very slow and linear compared to everything else i am working with
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: c10h12n2o]
#23773666 - 10/26/16 03:26 PM (7 years, 3 months ago) |
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Ya thats like that lipstick mold ive only seen that in grain weird haha i was having problems cloning also took three weeks to get a descent growth ... rip the mushroom in half and take your sample if you have not i was cutting the stem then taking a sample and tbat was no bueno ...
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kingkc
enthusiast


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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: Boogieman47]
#23773772 - 10/26/16 04:00 PM (7 years, 3 months ago) |
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Those look to have encountered some mutant inducing shroud of incandescent radiation. looks good otherwise..peace
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wtfcrazymofo
foil hater



Registered: 07/26/15
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Re: First PE: Tubs, Remove Blobs Before Casing? [Re: c10h12n2o]
#23773988 - 10/26/16 05:05 PM (7 years, 3 months ago) |
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Quote:
c10h12n2o said: thanks again for the advice buddy!! 
... Wow you sure hook it up with the high fives. I can't wait to see you pop that cherry. Keep cloning.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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