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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Last pic that slimy grain looks bacterial.
and about uploading pics:
https://www.shroomery.org/5286/How-do-I-put-pictures-in-my-post
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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just_curious
Cultivator


Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 6 months
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Re: First time grower facing a decision [Re: Tira]
#23676700 - 09/25/16 12:52 PM (7 years, 4 months ago) |
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2.5cc for grain inoculation, yikes! It's unadvised to even use MS to noc up grains, but if you do, you want to use the least amount of solution as possible...probably like .5cc.
I suggest you start working with agar, as you may see contam'd jars here soon. With the extra moisture that you put in the jar with 2.5cc, also the fact that you don't know for sure that the syringe is clean, and any bacteria that could have remained in your grains after prep, there is a moderate probability. At least when working with agar, you can spot any bacterial spots, you can transfer until it's perfectly clean, and drop a whole wedge inside your jar via "tiger drop". All while maintaining the original moisture content inside of your jars.
Good luck though, I hope you see growth soon.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First time grower facing a decision [Re: just_curious]
#23676722 - 09/25/16 01:01 PM (7 years, 4 months ago) |
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who uploads to photobucket anymore?? geezzz just upload to shroomery most people dont click outside links.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First time grower facing a decision [Re: mushboy]
#23677802 - 09/25/16 07:53 PM (7 years, 4 months ago) |
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None of those are working correctly.
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: First time grower facing a decision [Re: Inocuole]
#23677961 - 09/25/16 08:35 PM (7 years, 4 months ago) |
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Quote:
Space Coast Slayer said:

FTFY
In the future use this to upload your images: https://www.shroomery.org/forums/upload.php?action=upload
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herrenvolk


Registered: 05/14/16
Posts: 222
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Quote:
Space Coast Slayer said: I inoculated through a self healing injection port about an inch off center of the lid and then shook the jars per the teks
WOAAAAAAH! You forgot to say "my friend"! YOU'RE GONNA GET BUSTED!!! AHHHHHHHHHHHHHHHHHHHHHHH!!!!!!!! YOU'RE DOOOMED!!!!!!!!!!!!
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: First time grower facing a decision [Re: herrenvolk]
#23678244 - 09/25/16 10:19 PM (7 years, 4 months ago) |
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He stopped doing that a while back. That reminds me. My dog needs to check on the tubs she's growing.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Mush Freak
Myco Psycho



Registered: 12/29/11
Posts: 154
Last seen: 5 years, 9 days
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Re: First time grower facing a decision [Re: NumeroEno]
#23678452 - 09/25/16 11:56 PM (7 years, 4 months ago) |
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If you want to see growth as soon as possible, as you are injecting the MS into the jar, tilt the jar at a 45 degree angle so the needle is pointing directly at the glass. As the spores/liquid are aspirated out of the needle they will hit the glass and run down the sides. Dont shake your grain after inoculation. If you do this you will see the spores growing up the side of the jar. Remember if you shoot your MS load down the middle of the jar, its all colonizing in the middle of the jar and can be very hard to see.
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 Mono-tubs are LIFE
  
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PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
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Re: First time grower facing a decision [Re: Mush Freak]
#23678873 - 09/26/16 06:22 AM (7 years, 4 months ago) |
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when you shake after inoculating, it spreads the spores out more, decreasing the probability they will find a suitable "mate". It looks like your grains are prepped well and your myc looks ok (from what I can see, except that last pic doesn't look promising). Give it like 4 more days and take more pics. My GT and TC grew so s l o w.....
P.S. I live in your area, just be glad your a/c didn't go out in June when we had that heat wave.
-------------------- I would like to acquire anything I don’t have
Edited by PortabellaFella 1 (09/26/16 06:30 AM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Yeah that last pic I wouldn't trust. Already looking bacterial on the bottom.
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Space Coast Slayer
Total Fucking Noob

Registered: 09/19/16
Posts: 158
Loc: I come from the water.
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Re: First time grower facing a decision [Re: herrenvolk]
#23679428 - 09/26/16 10:45 AM (7 years, 4 months ago) |
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Quote:
herrenvolk said:
Quote:
Space Coast Slayer said: I inoculated through a self healing injection port about an inch off center of the lid and then shook the jars per the teks
WOAAAAAAH! You forgot to say "my friend"! YOU'RE GONNA GET BUSTED!!! AHHHHHHHHHHHHHHHHHHHHHHH!!!!!!!! YOU'RE DOOOMED!!!!!!!!!!!!
-------------------- Everything I post is pure fiction and all images were stolen from google.
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Space Coast Slayer
Total Fucking Noob

Registered: 09/19/16
Posts: 158
Loc: I come from the water.
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2 of my jars are doing great from what my untrained eye can tell but two of them are languishing behind..
The two that are doing well have colonized 30-40% of what I can see against the glass but not in one spot.. That's around the entire outside of the jar.. So there's no telling how much has colonized within where I can't see.. It's all tomentose growth with no rhizomorphic growth that I can see, not sure if that makes a diff..
Should I go ahead and give those two a shake anyway even though they might be past 30%? Are there any complications that may arise from not shaking? I've seen arguements for both, but most eventually concur on the shake..
-------------------- Everything I post is pure fiction and all images were stolen from google.
Edited by Space Coast Slayer (09/28/16 06:11 PM)
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PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
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Yes shake around 30%, I'd shake the other ones too but that's just me.
-------------------- I would like to acquire anything I don’t have
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Space Coast Slayer
Total Fucking Noob

Registered: 09/19/16
Posts: 158
Loc: I come from the water.
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The two good jars look like this on all sides

The two lagging jars are displaying about 10% colonization against the glass.. Why would they lag behind in the same conditions as the good ones? Only logical answer is contamination, no?
-------------------- Everything I post is pure fiction and all images were stolen from google.
Edited by Space Coast Slayer (09/28/16 08:28 PM)
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PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
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I had 9 jars, all inoculated at the same time. Each one finished at a different time. Contam, genetics, too wet, too dry...... Could be a host of different reasons. As long as they look healthy with no odd colors, slime or cottonball like growth, it should be ok. Multispore syringes...... Now we know why the successful growers here do MS->grow->clone with agar->and isolate.
Edit: the growth could also be in the center and just showing a little on the glass
-------------------- I would like to acquire anything I don’t have
Edited by PortabellaFella 1 (09/29/16 07:31 AM)
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Space Coast Slayer
Total Fucking Noob

Registered: 09/19/16
Posts: 158
Loc: I come from the water.
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Just had a quick question.. Do you guys think one 6500k cfl bulb is enough light for two trays in this 106 quart SGFC? Seems like it illuminates it well upon testing..
Edit: I know it looks like Stevie Wonder laid out my grid for the holes
-------------------- Everything I post is pure fiction and all images were stolen from google.
Edited by Space Coast Slayer (10/03/16 04:24 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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thats a big ass sgfc,
and yes. plenty of light!
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First time grower facing a decision [Re: mushboy]
#23704224 - 10/03/16 04:30 PM (7 years, 3 months ago) |
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just realized,trays in a sgfc? ive done it but with much taller chambers.
you'll need about 2inches of perlite 2-3inches of spawn/sub in a tray 4-8inches for mushies...
looks like you might run out of room.
ive been doing trays in mono tubs. im diggin it.
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Space Coast Slayer
Total Fucking Noob

Registered: 09/19/16
Posts: 158
Loc: I come from the water.
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Re: First time grower facing a decision [Re: mushboy]
#23704234 - 10/03/16 04:34 PM (7 years, 3 months ago) |
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Yeah its a little big but its perfect size to hold two trays.. Im colonizing rye berries and plan to spawn to manure based substrate using the trays pictured...
Can you fruit a tray in a SGFC? I naturally assumed yes but did not think to ask someone who actually knows wtf they are doing..
-------------------- Everything I post is pure fiction and all images were stolen from google.
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Space Coast Slayer
Total Fucking Noob

Registered: 09/19/16
Posts: 158
Loc: I come from the water.
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Re: First time grower facing a decision [Re: mushboy]
#23704244 - 10/03/16 04:37 PM (7 years, 3 months ago) |
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Quote:
mushboy said: just realized,trays in a sgfc? ive done it but with much taller chambers.
you'll need about 2inches of perlite 2-3inches of spawn/sub in a tray 4-8inches for mushies...
looks like you might run out of room.
ive been doing trays in mono tubs. im diggin it.
I gotcha.. The chamber is 13" tall... 2" of perlite plus 3" deep trays leaves 8"... Hopefully it will suffice.. Thanks for the insight sir..
-------------------- Everything I post is pure fiction and all images were stolen from google.
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