|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Trippy_clayts
in need of mentor



Registered: 07/29/16
Posts: 39
Loc: hawaii
Last seen: 6 years, 2 months
|
From an agar isolate how many g2g transfers are possible from a half pint WBS
#23667359 - 09/22/16 07:58 AM (7 years, 4 months ago) |
|
|
Recently I made an agar isolate from a clone and then transferred to another petri,from there I cut out my wedge and put into a very small amount (about 1/4 pint of Wbs.following that I transferred to about 1lb of Wbs in a wbs bag,it is growing quite quickly and producing strong myc.would it be smart to use that as a master wbs bag? If so how many more 1lb bag would I be able to further inoculate without weakening or diluting the isolate?
-------------------- i wish everybody would just eat shrooms and understand...its that easy
|
Morel Guy
Stranger


Registered: 01/23/13
Posts: 15,577
Last seen: 4 years, 1 month
|
Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: Trippy_clayts]
#23667390 - 09/22/16 08:19 AM (7 years, 4 months ago) |
|
|
I am also curious.
A slant should of been made so a Petri dish could be grown at any time to start master spawns. I don't know how many master spawn can be replicated from master to master.
-------------------- "in sterquiliniis invenitur in stercore invenitur" In filth it will be found in dung it will be found
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: Morel Guy]
#23667410 - 09/22/16 08:34 AM (7 years, 4 months ago) |
|
|
that bag doesn't look 100% healthy.
if ur going to use bags, go for at least 4# of grain. u can already fit 1# in a standard sized qt jar.
people usually stop after G3. for example, ur one pint jar (G1)>1# bag(G2)>X amount of bags (G3)>spawn these to bulk
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
Trippy_clayts
in need of mentor



Registered: 07/29/16
Posts: 39
Loc: hawaii
Last seen: 6 years, 2 months
|
Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: blindingleaf]
#23667595 - 09/22/16 10:13 AM (7 years, 4 months ago) |
|
|
The bag is not yet fully colonized blindingleaf I did the transfer 3 days ago
-------------------- i wish everybody would just eat shrooms and understand...its that easy
|
Trippy_clayts
in need of mentor



Registered: 07/29/16
Posts: 39
Loc: hawaii
Last seen: 6 years, 2 months
|
Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: Trippy_clayts]
#23667599 - 09/22/16 10:15 AM (7 years, 4 months ago) |
|
|
Okay morel guy,i shall now start studying slants! Thanks for your input
-------------------- i wish everybody would just eat shrooms and understand...its that easy
|
NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
|
Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: Trippy_clayts]
#23667615 - 09/22/16 10:24 AM (7 years, 4 months ago) |
|
|
The way I do it is I drop a wedge into a pint of millet, then g2g that pint into 4-8 more, depending on how full it is, then use each of those pints to inoculate a ~5 quart spawn bag. I've never weighed the bags but I'd guesstimate them to be about 5lbs.
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
|
Trippy_clayts
in need of mentor



Registered: 07/29/16
Posts: 39
Loc: hawaii
Last seen: 6 years, 2 months
|
Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: NumeroEno]
#23675781 - 09/25/16 03:19 AM (7 years, 4 months ago) |
|
|
right on numero.thanks man
-------------------- i wish everybody would just eat shrooms and understand...its that easy
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 372 topic views. 27 members, 169 guests and 55 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|