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OfflineTrippy_clayts
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From an agar isolate how many g2g transfers are possible from a half pint WBS
    #23667359 - 09/22/16 07:58 AM (7 years, 4 months ago)

Recently I made an agar isolate from a clone and then transferred to another petri,from there I cut out my wedge and put into a very small amount (about 1/4 pint of Wbs.following that I transferred to about 1lb of Wbs in a wbs bag,it is growing quite quickly and producing strong myc.would it be smart to use that as a master wbs bag? If so how many more 1lb bag would I be able to further inoculate without weakening or diluting the isolate?


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i wish everybody would just eat shrooms and understand...its that easy


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OfflineMorel Guy
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Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: Trippy_clayts]
    #23667390 - 09/22/16 08:19 AM (7 years, 4 months ago)

I am also curious. 

A slant should of been made so a Petri dish could be grown at any time to start master spawns.  I don't know how many master spawn can be replicated from master to master.


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"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found


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Invisibleblindingleaf
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Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: Morel Guy]
    #23667410 - 09/22/16 08:34 AM (7 years, 4 months ago)

that bag doesn't look 100% healthy.

if ur going to use bags, go for at least 4# of grain. u can already fit 1# in a standard sized qt jar.

people usually stop after G3.  for example, ur one pint jar (G1)>1# bag(G2)>X amount of bags (G3)>spawn these to bulk


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineTrippy_clayts
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Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: blindingleaf]
    #23667595 - 09/22/16 10:13 AM (7 years, 4 months ago)

The bag is not yet fully colonized blindingleaf I did the transfer 3 days ago


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i wish everybody would just eat shrooms and understand...its that easy


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OfflineTrippy_clayts
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Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: Trippy_clayts]
    #23667599 - 09/22/16 10:15 AM (7 years, 4 months ago)

Okay morel guy,i shall now start studying slants! Thanks for your input


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i wish everybody would just eat shrooms and understand...its that easy


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InvisibleNumeroEno
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Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: Trippy_clayts]
    #23667615 - 09/22/16 10:24 AM (7 years, 4 months ago)

The way I do it is I drop a wedge into a pint of millet, then g2g that pint into 4-8 more, depending on how full it is, then use each of those pints to inoculate a ~5 quart spawn bag. I've never weighed the bags but I'd guesstimate them to be about 5lbs.


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineTrippy_clayts
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Re: From an agar isolate how many g2g transfers are possible from a half pint WBS [Re: NumeroEno]
    #23675781 - 09/25/16 03:19 AM (7 years, 4 months ago)

right on numero.thanks man


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i wish everybody would just eat shrooms and understand...its that easy


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