Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offline1st
Mystic
Male


Registered: 01/22/16
Posts: 36
Last seen: 8 months, 13 days
Bad monotub growth? First grow
    #23666235 - 09/21/16 08:15 PM (7 years, 4 months ago)

Hi there,

Ive been having a crack at growing and have used the V-Tek Grass seed method.

Originally I went for MS inoculation -  500ml RGS with 2cc from a syringe I made myself.

I am using a glove box (tote with holes) and isopropyl combined with flame sterilisation for the syringe and spore scraper (flathead bit)

I also innoced 5 containers (250ml of RGS in 500 ml containers) with 1cc each.

They colonised to about 60% each and then i shook them and then they colonised about 90% again and at which point i used 5 of the 250ml to mix with 50/50 and the single 500ml to mix with 50/50

I have also started to try WBS and injected 5cc of MS into a 1 litre jar filled 1/2. Its been over a week with no grow but i am not sure if it is due to low temp or not, proobably.

Here are some pics so you can see. Please let me know what you think of the myc growth on the monos. Thank you :smile: Your feedback is appreciated.

Small containers



My 50/50


Monos x 2




Extras: Filter Print Post Top
Offlinetump
ban the undead
 User Gallery


Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
Re: Bad monotub growth? First grow [Re: 1st]
    #23666265 - 09/21/16 08:27 PM (7 years, 4 months ago)

It doesn't look awful


Extras: Filter Print Post Top
Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot


Registered: 07/06/16
Posts: 988
Re: Bad monotub growth? First grow [Re: tump]
    #23666293 - 09/21/16 08:34 PM (7 years, 4 months ago)

Some quick questions:

A) Did you wait until the jars were fully colonized before you spawned them to your bulk substrate?

B) Did you wipe your syringes before or after you flamed the needle?

C) Did you follow Damion5050's coir tek or did you use 50/50 (Coir/Vermiculite)? (Damion5050 is the username - not the recipe...)

1 week is not long enough to worry about spore growth. Spore germination can sometimes take longer than only 1 week.

Thanks!


Extras: Filter Print Post Top
Offline1st
Mystic
Male


Registered: 01/22/16
Posts: 36
Last seen: 8 months, 13 days
Re: Bad monotub growth? First grow [Re: morty422]
    #23666351 - 09/21/16 08:54 PM (7 years, 4 months ago)

Quote:

morty422 said:
Some quick questions:

A) Did you wait until the jars were fully colonized before you spawned them to your bulk substrate?

B) Did you wipe your syringes before or after you flamed the needle?

C) Did you follow Damion5050's coir tek or did you use 50/50 (Coir/Vermiculite)? (Damion5050 is the username - not the recipe...)

1 week is not long enough to worry about spore growth. Spore germination can sometimes take longer than only 1 week.

Thanks!




Hey thank you for your reply.

Yep I waited about25 days for them to colonise after shaking once and then once more just prior to adding to 50/50

With the syringe, I wiped them with alcohol then flamed and then shot out a drop or two to cool the tip and then inoculated, i didn't wipe after flaming

I'm pretty sure i followed the coir tek, just got a coir brick (1/3) and 2 litres of verm and 4 litres of water. It was a bit past FC so i added another litre of verm to even it out. Let it sit for a week because the jars hadnt colonise. The 2nd tub i used a bucket coir tek with the same recipe and let it sit in there with the boiling water about 1 hr, then stirred then let sit for 4 then stirred again and put in a storage container for two days then spawned the 5 jars into it.

I'll wait as long as i have to, hopefully i can up the temps in this room and see what happens, i'll give it another 15-20 days before fruiting. Hopefully she'll be right :laugh:.


Extras: Filter Print Post Top
Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot


Registered: 07/06/16
Posts: 988
Re: Bad monotub growth? First grow [Re: 1st]
    #23666835 - 09/21/16 11:48 PM (7 years, 4 months ago)

Damion's Tek is measured in quarts...however- litres are extremely similar-so I don't think the harm is there in any way.

Your tubs do not look terrible. I am sort of young at indoor cultivation-and I've had my fair share of "OMG-This looks contaminated" and many others have pointed me in the right direction.

Here is what you need to know about inoculating grain jars from syringe:

-The syringe is most likely not 'perfectly clean' so there may be some bacteria riding along in the solution with the spores. This does not mean that your tub is going to turn green immediately and start to sprout patches of trich-trees... This just means that the overall efficiency of the spawn and substrate in general may be diminished. You might see lower yields than our comrades who have used agar to get rid of said bacteria.

-Multi-spore (syringe) grows contain a very diverse amount of genetics... There are an extremely large amount of sexy-times going on in those jars and you never really know what might come out of them.
If you spawn an entire tub (or mini-tub) with a MS syringe, you may deal with some of these issues: Uneven flushing patterns, Various different types of mutations, Bacterial spawn, Aborts regardless of conditions, etc...
With MS- there is far too many variables to create a hypothesis on why things happen, unless the problem is happening with EVERY SINGLE FRUITING BODY in the tub. (For example - the entire tub has massive amounts of 'fuzzy feet' = You might need more fresh air exchange... etc...)
---------------------------------------------
As of right now, your growth does not look terrible... It looks exactly like the growth I have seen on my own MS tubs...

Make sure to remember that FAE is the MAIN thing that fruiting bodies need. Humidity and Light are secondary.

As far as the sterility during spawn and fruiting is concerned - I'll give you a quote from RR that I feel is important:

"When it comes to spawning a bulk substrate, who gives a shit if you pick your nose while working? It doesn't matter. If the grains were properly prepared and colonized in sterile conditions to full colonization, you can spawn to bulk outdoors if you want to. .....However, you can bet your ass the spawn wasn't produced outdoors in the open air."

*Neither spawning or fruiting are sterile events-it's all in the cleanliness of your spawn that matters.*

And with coir - Any contaminates you see in your tub-have come from your spawn. Coir is one of the substrates that just won't contam by itself. Coir is used as a bedding for many different animals and they shit and piss on it all day. It never contams.

SIDE NOTE:
Coir is just as nutritious as manure in many ways...To say that it contains no beneficial nutrients is complete and utter bullshit...

:cheers:

I hope the best in your future grows!


Extras: Filter Print Post Top
Offline1st
Mystic
Male


Registered: 01/22/16
Posts: 36
Last seen: 8 months, 13 days
Re: Bad monotub growth? First grow [Re: morty422]
    #23669113 - 09/22/16 07:18 PM (7 years, 4 months ago)

Thanks man, very valuable info, much appreciated. I'll post the results eventually hehe :smile:

Peace


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* Best bulk substrate and casing? mycologist 11,591 8 09/09/01 04:47 PM
by Shdwstr
* Marijuana Seed Substrate
( 1 2 all )
Eightball 4,791 34 09/26/01 09:49 PM
by Jared
* fav. strain to grow Rashiki 2,890 11 10/03/01 02:19 PM
by Fried Brains
* There is no such thing as a good or bad way to gro Smallputrid 441 2 05/14/01 01:16 PM
by Smallputrid
* Outdoor grow with spent bulk substrate question Indian 1,771 11 05/18/18 10:29 AM
by grainbrain
* Using RICE MILK to grow mycelium/mushrooms??????
( 1 2 all )
jokerGD 4,883 24 03/05/18 10:19 AM
by Shroomarican
* Wedge of light growth on agar
( 1 2 all )
Parafaragaramus 1,187 27 03/05/18 09:28 PM
by Mr.Kaption
* Signs Of Growth.. have Questions indicaz 1,600 2 05/02/02 08:13 PM
by indicaz

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
344 topic views. 27 members, 190 guests and 43 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.008 seconds on 14 queries.