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catsfred7
Stranger
Registered: 09/01/16
Posts: 17
Last seen: 7 years, 13 days
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Microfiber tape before or after sterilization?
#23665897 - 09/21/16 06:13 PM (7 years, 4 months ago) |
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Do I put the microfiber tape on top my inoculation holes before or after sterilization in a pot with lid?
Will the tape still be on after all the heat?
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Microfiber tape before or after sterilization? [Re: catsfred7]
#23665926 - 09/21/16 06:27 PM (7 years, 4 months ago) |
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I put them on after.
However, I always put aluminum foil over my jars as I PC them, so no nasties fall on the top. I leave the jars inside the PC til cool, and then transfer directly to the SAB. I then remove the foil, flame sterilize my syringe outside of the SAB and carefully inoculate a jar. I flame sterilize between each jar as well.
Then while the jar is still inside, I cut a piece of micropore tape off the roll I already have ready and put it on.
Some people may do it differently, but this is how I do it and it works well. (Also use a 1/4" GE hole stuffed with poly. For a more "sterile" procedure, use sterile filter discs and self healing injection ports instead. But I'm cheap like that.)
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Microfiber tape before or after sterilization? [Re: Mushierage]
#23665931 - 09/21/16 06:28 PM (7 years, 4 months ago) |
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Sterile filter discs?
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Microfiber tape before or after sterilization? [Re: Inocuole]
#23665960 - 09/21/16 06:42 PM (7 years, 4 months ago) |
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Synthetic I mean of course.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Microfiber tape before or after sterilization? [Re: catsfred7]
#23665982 - 09/21/16 06:49 PM (7 years, 4 months ago) |
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Quote:
catsfred7 said: Do I put the microfiber tape on top my inoculation holes before or after sterilization in a pot with lid?
Will the tape still be on after all the heat?
before. the entire point of it is to keep moisture that accumulates between the lid and the foil from entering the jar.
putting it on afterwards is completely pointless and without merit.
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