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Offlinecatsfred7
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Registered: 09/01/16
Posts: 17
Last seen: 6 years, 11 months
How do I sterilize needle properly for inoculation?
    #23665768 - 09/21/16 05:22 PM (7 years, 4 months ago)

Last time I sterilized a spore syringe needle it turned the needle black from the flame from my lighter.

Does it matter if I wash this black stuff off ? I used a lighter or would an alcohol burner be better
Or does it not matter?

So let's say I use a bic lighter and the needle turns black. Should i wipe the black off with bleach after the needle turns red? And do this between every cake in shooting up?


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Invisiblerjstim
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Registered: 09/09/16
Posts: 103
Re: How do I sterilize needle properly for inoculation? [Re: catsfred7]
    #23665784 - 09/21/16 05:29 PM (7 years, 4 months ago)

I wouldn't wipe it off. It should theoretically be the most sterile immediately after the flame comes off.


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OfflineLeftfield420
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Registered: 02/26/16
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Re: How do I sterilize needle properly for inoculation? [Re: catsfred7]
    #23665817 - 09/21/16 05:42 PM (7 years, 4 months ago)

Butane torch lighter.....works like a charm....heat til needle is orange then cool....man use the search engine


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OfflineMushierage
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Registered: 06/29/16
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Re: How do I sterilize needle properly for inoculation? [Re: Leftfield420]
    #23665911 - 09/21/16 06:19 PM (7 years, 4 months ago)

Yes, try using something that burns clean, like an alcohol lamp or a blowtorch.  If you absolutely have to use a cigarette lighter, use the absolute tip of the flame heat up your syringe, so most of the gas burns away and doesn't leave a bunch of carbon on your tip. 

Same goes for agar scalpels/knives.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Offlinespacechildo
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Registered: 01/24/13
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Re: How do I sterilize needle properly for inoculation? [Re: catsfred7]
    #23665979 - 09/21/16 06:48 PM (7 years, 4 months ago)

soot doesnt hurt anything, lighters are fine for syringes but if you start dealing with scalpels, loops etc get a proper torch!


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InvisibleInocuole
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Re: How do I sterilize needle properly for inoculation? [Re: spacechildo]
    #23665999 - 09/21/16 06:55 PM (7 years, 4 months ago)

These two questions you recently posted were simple enough to be in a Q&A thread vs needing their own threads.


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