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Offlinejust_curious
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Exceeding the time for PCing honey water for LC
    #23665431 - 09/21/16 03:32 PM (7 years, 4 months ago)

So I'm looking at creamcorns TEK to make a LC (https://www.shroomery.org/forums/showflat.php/Cat/0/Number/5874305)and it says PC for 15 minutes at 15psi, do not exceed 15 minutes.

Then I'm also looking at the grocery store agar TEK (https://www.shroomery.org/forums/showflat.php/Number/7081785) that I will be placing in pasty plates and it says PC at 15psi for 20-25 minutes.

I have 2 honey water jars and 10 pasty plates that I just prepped and ready to PC. I figured that I would just PC them for the same amount of time, but this is my first time making LC solution. The whole "do not exceed" is throwing me off. I'm wondering to myself what the harm could be, and thinking maybe the sugar Caramalizes lol. But, at the same time, usually I just roll with the recipe and PC times from pasty's easy agar TEK and PC for 40-45 minutes.

If I PC my honey water for 20-25 minutes, will it do any harm. And, if I only PC my agar for 20-25 minutes instead of my usual 40-45 minutes, am I setting myself up for failure?

Thanks for your input.


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OfflineTira
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Re: Exceeding the time for PCing honey water for LC [Re: just_curious]
    #23665450 - 09/21/16 03:40 PM (7 years, 4 months ago)

Yes, sugar does caramelize.  Just follow the tek.

Why are you doing LC anyway? Why not GLC?


--------------------
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Offlinejust_curious
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Re: Exceeding the time for PCing honey water for LC [Re: Tira]
    #23665471 - 09/21/16 03:46 PM (7 years, 4 months ago)

I'm expanding one of my oyster LC syringes that I had, and putting a piece of colonized agar to the other for my cubes. I'm just now diving into it.


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OfflineTira
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Re: Exceeding the time for PCing honey water for LC [Re: just_curious]
    #23665486 - 09/21/16 03:50 PM (7 years, 4 months ago)

Personally I'd go with GLC by making a small jar, letting it colonize, then making GLC from it.  That way you can see if there's any contamination in there before using it.

But if you're going to go with regular LC, don't overdo PC; and read more before you start so you're sure of what you're doing .) Good luck.


--------------------
Useful Links for Beginners

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Agar for guaranteed spawn,
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OfflineMushierage
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Re: Exceeding the time for PCing honey water for LC [Re: Tira]
    #23665533 - 09/21/16 04:07 PM (7 years, 4 months ago)

If it's oyster, it honestly probably doesn't even really matter. 

Oyster mycelium can overcome most contaminations by...you guessed it, eating it.  Nom nom nom.

But yeah, this forum is for cultivating actives anyway. 


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Offlinejust_curious
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Re: Exceeding the time for PCing honey water for LC [Re: Tira]
    #23665573 - 09/21/16 04:20 PM (7 years, 4 months ago)

I've read multiple different threads with multiple time difference. I did 4 grams of honey to 96 ml of water. So my honey is 4% of its total weight. I'm raising the heat in my PC without the weight on, and as soon as the  steam starts coming I'll put the weight on. That way I brought the temp up slowly. I'm going for 20 minutes. Hopefully that will be the happy-medium time where the honey didn't caramelize and the agar PC'ed a good enough amount.


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Offlinejust_curious
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Re: Exceeding the time for PCing honey water for LC [Re: just_curious]
    #23665581 - 09/21/16 04:23 PM (7 years, 4 months ago)

Quote:

Mushierage said:
If it's oyster, it honestly probably doesn't even really matter. 

Oyster mycelium can overcome most contaminations by...you guessed it, eating it.  Nom nom nom.

But yeah, this forum is for cultivating actives anyway. 




Good thing this question was directed for the work with cubes then, huh.


Quote:

just_curious said:
I'm expanding one of my oyster LC syringes that I had, and putting a piece of colonized agar to the other for my cubes. I'm just now diving into it.




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OfflineMushierage
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Re: Exceeding the time for PCing honey water for LC [Re: just_curious]
    #23665598 - 09/21/16 04:26 PM (7 years, 4 months ago)

Uh huh.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Offlinejust_curious
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Re: Exceeding the time for PCing honey water for LC [Re: Mushierage]
    #23665632 - 09/21/16 04:35 PM (7 years, 4 months ago)

Quote:

Mushierage said:
Uh huh.



:grin:

Quote:

Tira said:
Personally I'd go with GLC by making a small jar, letting it colonize, then making GLC from it.  That way you can see if there's any contamination in there before using it.

But if you're going to go with regular LC, don't overdo PC; and read more before you start so you're sure of what you're doing .) Good luck.




Maybe I have a wrong way of thinking, but I feel like with GLC, you're also assuming that it's completely bacteria free.


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OfflineTira
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Re: Exceeding the time for PCing honey water for LC [Re: just_curious]
    #23665659 - 09/21/16 04:42 PM (7 years, 4 months ago)

Quote:

just_curious said:
Maybe I have a wrong way of thinking, but I feel like with GLC, you're also assuming that it's completely bacteria free.




Except you can observe bacterial growth in grain jars and can't observe it in honeywater LC.

So, no; you're not assuming, you'll know for sure if you know how to look for bacteria.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


Edited by Tira (09/21/16 04:43 PM)


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InvisibleInocuole
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Re: Exceeding the time for PCing honey water for LC [Re: Tira]
    #23665683 - 09/21/16 04:51 PM (7 years, 4 months ago)

Quote:

Tira said:
Why are you doing LC anyway? Why not GLC?




Because soaking up all the bacteria present in grains and injecting them into something else is a bit of a piss poor effort when there are cleaner ways to expand myc.


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OfflineTira
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Re: Exceeding the time for PCing honey water for LC [Re: Inocuole]
    #23665730 - 09/21/16 05:10 PM (7 years, 4 months ago)

Quote:

Inocuole said:
Because soaking up all the bacteria present in grains and injecting them into something else is a bit of a piss poor effort when there are cleaner ways to expand myc.




So, are you saying that, one can't achieve clean grain spawn no matter what?


--------------------
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Offlinejust_curious
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Re: Exceeding the time for PCing honey water for LC [Re: Tira]
    #23665797 - 09/21/16 05:35 PM (7 years, 4 months ago)

No, but there is typically SOME bacteria present in a colonized jar of grain. Whereas I can pull clean myc from agar and know that it is that much closer to being more effective than the GLC.


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Offlinejust_curious
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Re: Exceeding the time for PCing honey water for LC [Re: just_curious]
    #23665803 - 09/21/16 05:37 PM (7 years, 4 months ago)

I can clean up agar...I can't clean up GLC to put to more GLC.


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OfflineTira
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Re: Exceeding the time for PCing honey water for LC [Re: just_curious]
    #23666988 - 09/22/16 01:36 AM (7 years, 4 months ago)

Quote:

just_curious said:
No, but there is typically SOME bacteria present in a colonized jar of grain. Whereas I can pull clean myc from agar and know that it is that much closer to being more effective than the GLC.



Quote:

just_curious said:
I can clean up agar...I can't clean up GLC to put to more GLC.




I'm ofcourse not comparing agar to GLC here.  I'm comparing LC to GLC and you also can't clean up LC.

Anyway, if Inocuole says it's a bad idea, it must be a bad idea; I don't have half the experience he has.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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