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OfflineRubiks Cubensis
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Registered: 09/07/16
Posts: 21
Last seen: 5 months, 14 days
First try week 5 question
    #23664763 - 09/21/16 11:30 AM (7 years, 4 months ago)

I followed the PF tek to an OCD T. Inoculated 12 jars (1/2 pt. wide mouth Kerr) with B+ cubes (well shaken spore solution) in a SAB all to an OCD T. Jars well sterilized. 1/4 in. verm and .25 cc per hole with 4 holes. Used BRF and room temp was 76 minimum at night and 83 max in the day. Never even needed to post a question because all of them have already been asked (probably even this one).

The only difficult part was getting spores (wont mention why but you can guess). Exhausted every sponsor to no avail. Finally had to resort to one on one networking and got spores after 6 weeks of hard work. If anyone else has this problem I recommend making friends via the front page of the internet.

No growth or contams as week 4 approached. Finally one jar showed growth. Now at week 5 that 1 jar is approaching full colonization. Nothing at all in the other 11. Still being patient.

My question: Is this unusual (1 out of 12) or not really? No contams at all in the other 11. Considering inoculating another round of jars. Looks like I may have one lonely cake in my PMP soon (more like a RMP with all the high end bells and whistles I decided to go with). I decided to wait till week 7 to "give up" and maybe week 12 to dump them if no progress. Is this a good plan?

Thanks for all the wisdom on this board!


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The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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OfflineDr Greenthumb
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Re: First try week 5 question [Re: Rubiks Cubensis]
    #23664800 - 09/21/16 11:43 AM (7 years, 4 months ago)

5 weeks and only 1 jar has any growth? That seems pretty unusual, but I haven't had a grow in over a year.

You mentioned an alternate means of obtaining a spore syringe, how did you obtain it and how much do you trust it?

I'd also just switch to a SGFC if you're doing cakes, much more simpler.


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Offlinemrmazdarx9
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Re: First try week 5 question [Re: Dr Greenthumb]
    #23664939 - 09/21/16 12:36 PM (7 years, 4 months ago)

Did you squirt the needle against the glass so you could see the solution coming out?


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OfflineTira
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Registered: 11/20/10
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Re: First try week 5 question [Re: mrmazdarx9]
    #23664985 - 09/21/16 12:52 PM (7 years, 4 months ago)

5 weeks is a long time for no growth, even if you inoculate the cakes right in the center, you should see growth in 5 weeks.  Guess it's the spore solution.  Didn't understand why you couldn't get from sponsors, guess you have your reasons.

If you have more syringes it's time to make new jars and if your only jar is fully colonized you can fruit it and get some spore prints and make some spore syringes that you can trust .)


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OfflineRubiks Cubensis
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Registered: 09/07/16
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Re: First try week 5 question [Re: Dr Greenthumb]
    #23664992 - 09/21/16 12:54 PM (7 years, 4 months ago)

I found the most highly rated person on that site and contacted them directly. Very trusted source there. Used encrypted chat and bitcoin. Arrived in a few days. I like the low maintenance with the PMP. I have air stones coming up through geolite. RH stays at a constant 99% no problem.


--------------------
The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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OfflineRubiks Cubensis
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Re: First try week 5 question [Re: mrmazdarx9]
    #23665000 - 09/21/16 12:57 PM (7 years, 4 months ago)

Sure did. Very OCD about it all. Flame and shake between each jar. The only corner I cut was using masking tape and left the hole with no micropore tape for GE.


--------------------
The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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Offlinemrmazdarx9
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Re: First try week 5 question [Re: Rubiks Cubensis]
    #23665001 - 09/21/16 12:57 PM (7 years, 4 months ago)

Quote:

Rubiks Cubensis said:
I found the most highly rated person on that site and contacted them directly. Very trusted source there. Used encrypted chat and bitcoin. Arrived in a few days. I like the low maintenance with the PMP. I have air stones coming up through geolite. RH stays at a constant 99% no problem.



None of that has anything to do with colonisation that's for fruiting and outdated, fruiting just needs sgfc.


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OfflineRubiks Cubensis
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Re: First try week 5 question [Re: mrmazdarx9]
    #23665099 - 09/21/16 01:38 PM (7 years, 4 months ago)

Correct but someone brought up the SGFC. I did a search and whether its outdated appears still up for debate by some. The arguments appear to be that the SHFC is cheaper but messier and requires more maintenance. I guess if it fails to fruit I will switch. I want to be able to be away for 3-4 days if needed and money was not really a consideration for me.


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The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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Offlinemrmazdarx9
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Re: First try week 5 question [Re: Rubiks Cubensis]
    #23665109 - 09/21/16 01:42 PM (7 years, 4 months ago)

No I thought you were saying you had your jars colonising in a pmp set up that's why I said its for fruiting lol


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OfflineTheStormsEye
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Re: First try week 5 question [Re: mrmazdarx9]
    #23665215 - 09/21/16 02:21 PM (7 years, 4 months ago)

Got any pics bruh..?
you should try out the sgfc for sure. it works well when done correctly
and it will help you learn keys components of the whole process
as well as learning about the life cycle of your fungi.


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              All mushrooms are edible.. some only once


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InvisibleInocuole
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Re: First try week 5 question [Re: Rubiks Cubensis]
    #23665234 - 09/21/16 02:28 PM (7 years, 4 months ago)

Quote:

Rubiks Cubensis said:
I guess if it fails to fruit I will switch.




:lol:  It's a little late by then..


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OfflineRubiks Cubensis
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Re: First try week 5 question [Re: Inocuole]
    #23703534 - 10/03/16 12:59 PM (7 years, 3 months ago)

Update: Now in week 7. Previously only 1/12 was showing signs of colonization. Now 3 more are starting to finally show signs at week 7. So 1/12 is %95 colonized (but also pinning). 3/12 just starting and 8/12 no growth but no contams. Looks like patience was the answer. May take 12 weeks at this rate.

Should I go ahead and dunk/birth the one that is already pinning at only %95 colonization or should I wait a week?

Pics of the 3 stages and my PMP:




--------------------
The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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OfflineMushierage
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Re: First try week 5 question [Re: Rubiks Cubensis]
    #23703549 - 10/03/16 01:06 PM (7 years, 3 months ago)

That is some awfully coarse vermiculite you are using there bud.  Perhaps that is not helping it colonize quicker.

The first jar is ready to birth it looks like, assuming that top layer is dry verm.  There is an invitro pin on it.


--------------------
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InvisiblemushboyMDiscord
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Re: First try week 5 question [Re: Mushierage]
    #23703559 - 10/03/16 01:09 PM (7 years, 3 months ago)

ditch the pmp thing. get a sgfc.

and those jars look dry as fuck.
try to dunk n fruit what you can but
start over. sorry.:crazy2:


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OfflineRubiks Cubensis
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Registered: 09/07/16
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Re: First try week 5 question [Re: Mushierage]
    #23703623 - 10/03/16 01:26 PM (7 years, 3 months ago)

Thanks, I used the exact brand I saw listed in a PF Tek somewhere. Maybe that is the issue. Got a better brand recommendation? Here is what I am using:


--------------------
The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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OfflineRubiks Cubensis
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Re: First try week 5 question [Re: mushboy]
    #23703642 - 10/03/16 01:30 PM (7 years, 3 months ago)

Thanks for the input. May go the SGFC route if this does not work out. I am sometimes on the road 3-4 days and wanted something as self sustaining as possible and with less mess... learning.


--------------------
The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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Offlinemrmazdarx9
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Re: First try week 5 question [Re: Rubiks Cubensis]
    #23704147 - 10/03/16 04:03 PM (7 years, 3 months ago)

Fine verm is better faster colonisation. Just chuck it in a blender next time get it fine but not powdered


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COCA GROWERS come here and share your knowledge
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:penis: need some supplies in the UK check Here or PM me:penis:
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If you use "SWIM" you should DROWN


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OfflineRubiks Cubensis
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Re: First try week 5 question [Re: mrmazdarx9]
    #23704628 - 10/03/16 06:40 PM (7 years, 3 months ago)

Thanks all for the advice. I read a lot about fine v.s. Medium Verm and went with Medium. Or at least I thought I did. Based on that picture above do I appear to have medium or Coarse? I got scared away from fine based on stories of it getting to compacted. Now I have it so coarse it seems to have dried up like a desert in my 30% humidity room. Now I have the FC (PMP) running and the humidity jumped up to 65% in the room.


--------------------
The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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OfflineRubiks Cubensis
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Registered: 09/07/16
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Re: First try week 5 question [Re: Rubiks Cubensis]
    #23705746 - 10/04/16 04:52 AM (7 years, 3 months ago)

I think what I will just do is blend up 50% into fine vermiculite and use the other 50% as medium coarse. When in doubt choose a middle way option.


--------------------
The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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OfflineRubiks Cubensis
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Registered: 09/07/16
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Re: First try week 5 question [Re: Rubiks Cubensis]
    #23791252 - 11/01/16 12:52 PM (7 years, 2 months ago)

Update: I previously reported that I thought i had a problem with this grow because it was going so slow 1 out of 12 at week 5. I am now almost at week 11. Yesterday I had 8 out of 12 showing growth. Another one just showed the first spot of growth this morning. I have feeling those last 3 may come around. Hopefully by week 12 or 13. Meanwhile my first cake of the batch is dead after 5 flushes.I just now put #2 and #3 in the FC. Never expected such a staggered slow flow.

What is the "world record" time between inoculation and colonization? At this pace if my last cake comes in and takes 3 weeks - 4 weeks to colonize (after first sign of growth) as most have I could have my final cake taking almost 4 months to colonize.

Never expected this.


--------------------
The nature of dualistic language is such that anything stated is both true and not true at the same time. Including this statement.
-Rubik's Cubensis



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