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OfflineTira
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Excess water in no-pour agar plates.
    #23664363 - 09/21/16 08:23 AM (7 years, 4 months ago)

Whatever I did, I couldn't get rid of the excess water in my agar plates after PCing.

 


I even tried putting folded paper towels and wrapping them with foil two times before putting them into PC but didn't do anything for me.  Paper towel came out soaking wet and there was water droplets on the lids.  And I did raise the temperature of my pressure cooker slowly and left them to cool overnight in the PC.


I know condensation usually is not a problem but these water droplets later drop on the agar and causes the mycelium to drift around on agar even when the plates are carefully handled.

Looks like this:


 


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OfflineBurberrySpores
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23664525 - 09/21/16 09:49 AM (7 years, 4 months ago)

When you take the plates into your SAB, do your sterile procedure, and when you open your lids, just tip the container and dump out the water 


I have been doing it this way for a wwwhile.  No problems


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Re: Excess water in no-pour agar plates. [Re: BurberrySpores]
    #23664534 - 09/21/16 09:53 AM (7 years, 4 months ago)

I don't get what you mean by excess water? All I have is agar on my plates and condensation if I leave them somewhere a little warm, is this just pasty plate related?


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: BurberrySpores]
    #23664540 - 09/21/16 09:56 AM (7 years, 4 months ago)

Quote:

BurberrySpores said:
When you take the plates into your SAB, do your sterile procedure, and when you open your lids, just tip the container and dump out the water 


I have been doing it this way for a wwwhile.  No problems





That may actually work, good idea.  But isn't there a way to avoid condensation in the first place?  I mean pouring the water out of plates is an extra step and even it sounds safe it does add risk of contamination to the process.


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
    #23664545 - 09/21/16 09:58 AM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
I don't get what you mean by excess water? All I have is agar on my plates and condensation if I leave them somewhere a little warm, is this just pasty plate related?




Look at the first two pictures in the OP

In the first pic you can see the water droplets on lids and in the second pic you can see condensation on the walls of the plates.  That condensation later drips down on agar and then I have water on my agar that drifts spores and mycelium all over.


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OfflineBurberrySpores
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23664559 - 09/21/16 10:04 AM (7 years, 4 months ago)

Quote:

Tira said:
Quote:

BurberrySpores said:
When you take the plates into your SAB, do your sterile procedure, and when you open your lids, just tip the container and dump out the water 


I have been doing it this way for a wwwhile.  No problems





That may actually work, good idea.  But isn't there a way to avoid condensation in the first place?  I mean pouring the water out of plates is an extra step and even it sounds safe it does add risk of contamination to the process.




There's always going to be the excess water, condensation, when doing no pours IME

When you open the lids in the SAB, just tip the container to the side and the water will pour right out.

Its so easy guys... :wellhellothere:


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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23664563 - 09/21/16 10:05 AM (7 years, 4 months ago)

So condensation then, it washes the mycelium off the agar?. How much are you handling the plates for it to drift it all about. I've not had myc move from the agar like you said, is your agar not too runny if the myc is drifting around?


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
    #23664567 - 09/21/16 10:07 AM (7 years, 4 months ago)

No agar is solid, it's just the water that drifts on it when handled for observation.


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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23664575 - 09/21/16 10:09 AM (7 years, 4 months ago)

Tira said:
Quote:

I have water on my agar that drifts spores and mycelium all over.




I'm just going off this


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InvisibleNumeroEno
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
    #23664583 - 09/21/16 10:12 AM (7 years, 4 months ago)

There's no way to prevent condensation in no pour plates, but you can leave them out on the shelf for a while and most of the condensation will dry out/get reabsorbed. If condensation is a problem for you, you might consider pouring petris. It's not as hard or intimidating as it seems, especially if you get the right bottle. Letting your agar cool in the bottle for a while before pouring and then stacking your plates after the pour virtually eliminates condensation on all but the top couple plates.


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Re: Excess water in no-pour agar plates. [Re: NumeroEno]
    #23664595 - 09/21/16 10:17 AM (7 years, 4 months ago)

Quote:

NumeroEno said:
There's no way to prevent condensation in no pour plates, but you can leave them out on the shelf for a while and most of the condensation will dry out/get reabsorbed. If condensation is a problem for you, you might consider pouring petris. It's not as hard or intimidating as it seems, especially if you get the right bottle. Letting your agar cool in the bottle for a while before pouring and then stacking your plates after the pour virtually eliminates condensation on all but the top couple plates.



:whathesaid:
I bought this bad boy media pouring borosilicate bottle


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
    #23664614 - 09/21/16 10:23 AM (7 years, 4 months ago)

I was actually thinking about switching to pour agar but was waiting to build a flowhood.  I don't know why but I just can't convince myself that I could do that successfully in a SAB.

You make it sound easy, maybe I should give it a try.

(After making a new SAB next week ofc, my current SAB is about 50-60 quarts nowhere near enough space in there. Gonna make a 120 quart one)


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InvisibleNumeroEno
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23664626 - 09/21/16 10:30 AM (7 years, 4 months ago)

Quote:

Tira said:
I was actually thinking about switching to pour agar but was waiting to build a flowhood.  I don't know why but I just can't convince myself that I could do that successfully in a SAB.

You make it sound easy, maybe I should give it a try.

(After making a new SAB next week ofc, my current SAB is about 50-60 quarts nowhere near enough space in there. Gonna make a 120 quart one)




I poured plates in my SAB for over a year before I built a flowhood. It's pretty easy honestly. Definitely easier than g2ging into a half gallon jar inside a SAB.


--------------------

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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23664628 - 09/21/16 10:31 AM (7 years, 4 months ago)

I've had good success with my 80 litre SAB don't know what that is in quarts off hand, but its plenty big enough I've so far only had 4 plates go wrong and im crap lol, two where my very first attempt and I was woefully unequipped.


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Re: Excess water in no-pour agar plates. [Re: NumeroEno]
    #23664642 - 09/21/16 10:35 AM (7 years, 4 months ago)

I've thought about getting a pyrex media bottle for pouring plates, but I still have this thing and it's awesome. I modified the spout a little bit by reaming out the tip to make it bigger, but this thing has kicked ass for almost 2 years now. Picked it up at world market for 10 bucks.



--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
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Offlinespacechildo
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Re: Excess water in no-pour agar plates. [Re: NumeroEno]
    #23664668 - 09/21/16 10:48 AM (7 years, 4 months ago)

that looks a lot like one of my favorite bongs through the years eno :super:

lazy mans tek for removing condensation:

1 leave the plates alone for 3 days after PC'ing.
2 nothing, you're done


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
    #23664717 - 09/21/16 11:07 AM (7 years, 4 months ago)

Quote:

spacechildo said:
that looks a lot like one of my favorite bongs through the years eno :super:

lazy mans tek for removing condensation:

1 leave the plates alone for 3 days after PC'ing.
2 nothing, you're done




my kinda tek .) will definitely try that. thanks a lot.

Quote:

mrmazdarx9 said:
I've had good success with my 80 litre SAB don't know what that is in quarts off hand, but its plenty big enough I've so far only had 4 plates go wrong and im crap lol, two where my very first attempt and I was woefully unequipped.




quarts and litre are almost the same as far as I know

thanks a lot guys, you really encouraged me, I think I'm gonna try pour agar soon .)


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Re: Excess water in no-pour agar plates. [Re: NumeroEno]
    #23664733 - 09/21/16 11:17 AM (7 years, 4 months ago)

Quote:

NumeroEno said:
I've thought about getting a pyrex media bottle for pouring plates, but I still have this thing and it's awesome. I modified the spout a little bit by reaming out the tip to make it bigger, but this thing has kicked ass for almost 2 years now. Picked it up at world market for 10 bucks.





That is cool to be fair


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23664772 - 09/21/16 11:34 AM (7 years, 4 months ago)

btw what do you think of these plates, can I do transfers from them? All growth on agar has discoloration beneath them which can be seen clearly from the bottom of the plates.  Is that bacteria? (these plates were made from a syringe that had a very high chance of being contaminated I made the syringes two years ago with zero sterile technique

 

Above is the only proper growth I have on agar that wasn't messed up by water drifting around. 

 

This one is pretty messy but I think there's a clear patch of mycelium at 8 o'clock and maybe at 5 o'clock?

 

This is the next best plate but it doesn't look very promising.

What do you guys think?


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OfflineLa Flama Blanca
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23664853 - 09/21/16 12:02 PM (7 years, 4 months ago)

Quote:

Tira said:
btw what do you think of these plates, can I do transfers from them? All growth on agar has discoloration beneath them which can be seen clearly from the bottom of the plates.  Is that bacteria? (these plates were made from a syringe that had a very high chance of being contaminated I made the syringes two years ago with zero sterile technique

 

Above is the only proper growth I have on agar that wasn't messed up by water drifting around. 

 

This one is pretty messy but I think there's a clear patch of mycelium at 8 o'clock and maybe at 5 o'clock?

 

This is the next best plate but it doesn't look very promising.

What do you guys think?




it helps to know what kind of spores germinated on those plates....not all myc growth is supposed to be white everytime. for example, PS. mexicana


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: La Flama Blanca]
    #23664946 - 09/21/16 12:38 PM (7 years, 4 months ago)

Quote:

La Flama Blanca said:


it helps to know what kind of spores germinated on those plates....not all myc growth is supposed to be white everytime. for example, PS. mexicana




I'm so used to cubes I didn't feel the need to say they were cubes .) planning on a galindoi grow soon, hope that'll change that

these are cubes, golden teacher


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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23665217 - 09/21/16 02:22 PM (7 years, 4 months ago)

in that case, personally i would not use. the contam on that plate is most likely bacteria but could be mold...either way, the contam on that plate is basically holding hands with the myc. isolating away from it would be more trouble for me than it'd be worth. you should also consider doing your cleaning transfers a couple days sooner, until you're sure the growth will be clean, otherwise you give the contams time to "out run" the cube myc.

i used to have the problem a lot though. i started vigorously shaking the plates showing excess moisture/condensation, so that the water would "clump up" in a pool, then dump that pool of water out. you'll still have a few excess drops of water in there, but they shouldn't be a problem really if your technique is good.


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23665736 - 09/21/16 05:12 PM (7 years, 4 months ago)

Quote:

Tira said:

 







this one actually looks kinda promising to me, the patch at 8 o'clock seems to have no bacteria under it.


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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23665848 - 09/21/16 05:54 PM (7 years, 4 months ago)

i'm very skeptical, why are there knots in the middle if that's healthy myc?
and the fuzzy weird look makes me suspect its mold, those 2 things combined tells me you should start over.

YOu could of course get lucky but I wouldn't bother, the chances are too small for this to be good IMO!


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: spacechildo]
    #23666942 - 09/22/16 12:43 AM (7 years, 4 months ago)

Quote:

spacechildo said:
i'm very skeptical, why are there knots in the middle if that's healthy myc?
and the fuzzy weird look makes me suspect its mold, those 2 things combined tells me you should start over.

YOu could of course get lucky but I wouldn't bother, the chances are too small for this to be good IMO!




I thought the yellow discoloration in the middle was caused by bacteria and those knot looking things were mycelium that couldn't grow because of bacteria.  Was I wrong?


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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23667602 - 09/22/16 10:16 AM (7 years, 4 months ago)

Quote:

Tira said:
Quote:

spacechildo said:
i'm very skeptical, why are there knots in the middle if that's healthy myc?
and the fuzzy weird look makes me suspect its mold, those 2 things combined tells me you should start over.

YOu could of course get lucky but I wouldn't bother, the chances are too small for this to be good IMO!




I thought the yellow discoloration in the middle was caused by bacteria and those knot looking things were mycelium that couldn't grow because of bacteria.  Was I wrong?




thats likely right, but does it really matter? that plate is gross, why do you so desperately want to work with it? if you feel so compelled to try and save it, you could do a hot pour ontop of it. RR has a good explanation of the tek to do it. essentially you pour hot agar on top of it, and watch the plate closely for a couple days, then as soon as you see myc growth on top of the new surface, ideally within a couple hours of the growth you gently scrape it off and put it on a new plate. requires too much babysitting imo tho.... i have better things to do than battle 1 heavily contaminated plate.


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OfflineTira
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Re: Excess water in no-pour agar plates. [Re: La Flama Blanca]
    #23667610 - 09/22/16 10:22 AM (7 years, 4 months ago)

Quote:

La Flama Blanca said:

thats likely right, but does it really matter? that plate is gross, why do you so desperately want to work with it? if you feel so compelled to try and save it, you could do a hot pour ontop of it. RR has a good explanation of the tek to do it. essentially you pour hot agar on top of it, and watch the plate closely for a couple days, then as soon as you see myc growth on top of the new surface, ideally within a couple hours of the growth you gently scrape it off and put it on a new plate. requires too much babysitting imo tho.... i have better things to do than battle 1 heavily contaminated plate.




like paul stamets' agar sandwich but without a transfer; interesting, thanks for sharing that.

I have other agar plates going on but these are my first plates and I really want to work on agar and get experience.  so why not do transfers from that plate while waiting for growth on others .)


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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23667656 - 09/22/16 10:48 AM (7 years, 4 months ago)

Its all practice at the end of the day


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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23668111 - 09/22/16 01:28 PM (7 years, 4 months ago)

Quote:

Tira said:
Quote:

La Flama Blanca said:

thats likely right, but does it really matter? that plate is gross, why do you so desperately want to work with it? if you feel so compelled to try and save it, you could do a hot pour ontop of it. RR has a good explanation of the tek to do it. essentially you pour hot agar on top of it, and watch the plate closely for a couple days, then as soon as you see myc growth on top of the new surface, ideally within a couple hours of the growth you gently scrape it off and put it on a new plate. requires too much babysitting imo tho.... i have better things to do than battle 1 heavily contaminated plate.




like paul stamets' agar sandwich but without a transfer; interesting, thanks for sharing that.

I have other agar plates going on but these are my first plates and I really want to work on agar and get experience.  so why not do transfers from that plate while waiting for growth on others .)




up to you man. i think most people, though, would recommend not bothering with advanced techniques like those until you've gotten some solid experience working with agar. building the foundation of the house before adding in the AC and whatnot. but by all means, dive in as deep as you want. :thumbup:


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