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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
Re: Excess water in no-pour agar plates. [Re: La Flama Blanca]
    #23664946 - 09/21/16 12:38 PM (7 years, 4 months ago)

Quote:

La Flama Blanca said:


it helps to know what kind of spores germinated on those plates....not all myc growth is supposed to be white everytime. for example, PS. mexicana




I'm so used to cubes I didn't feel the need to say they were cubes .) planning on a galindoi grow soon, hope that'll change that

these are cubes, golden teacher


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OfflineLa Flama Blanca
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23665217 - 09/21/16 02:22 PM (7 years, 4 months ago)

in that case, personally i would not use. the contam on that plate is most likely bacteria but could be mold...either way, the contam on that plate is basically holding hands with the myc. isolating away from it would be more trouble for me than it'd be worth. you should also consider doing your cleaning transfers a couple days sooner, until you're sure the growth will be clean, otherwise you give the contams time to "out run" the cube myc.

i used to have the problem a lot though. i started vigorously shaking the plates showing excess moisture/condensation, so that the water would "clump up" in a pool, then dump that pool of water out. you'll still have a few excess drops of water in there, but they shouldn't be a problem really if your technique is good.


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23665736 - 09/21/16 05:12 PM (7 years, 4 months ago)

Quote:

Tira said:

 







this one actually looks kinda promising to me, the patch at 8 o'clock seems to have no bacteria under it.


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Offlinespacechildo
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23665848 - 09/21/16 05:54 PM (7 years, 4 months ago)

i'm very skeptical, why are there knots in the middle if that's healthy myc?
and the fuzzy weird look makes me suspect its mold, those 2 things combined tells me you should start over.

YOu could of course get lucky but I wouldn't bother, the chances are too small for this to be good IMO!


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
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Re: Excess water in no-pour agar plates. [Re: spacechildo]
    #23666942 - 09/22/16 12:43 AM (7 years, 4 months ago)

Quote:

spacechildo said:
i'm very skeptical, why are there knots in the middle if that's healthy myc?
and the fuzzy weird look makes me suspect its mold, those 2 things combined tells me you should start over.

YOu could of course get lucky but I wouldn't bother, the chances are too small for this to be good IMO!




I thought the yellow discoloration in the middle was caused by bacteria and those knot looking things were mycelium that couldn't grow because of bacteria.  Was I wrong?


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OfflineLa Flama Blanca
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23667602 - 09/22/16 10:16 AM (7 years, 4 months ago)

Quote:

Tira said:
Quote:

spacechildo said:
i'm very skeptical, why are there knots in the middle if that's healthy myc?
and the fuzzy weird look makes me suspect its mold, those 2 things combined tells me you should start over.

YOu could of course get lucky but I wouldn't bother, the chances are too small for this to be good IMO!




I thought the yellow discoloration in the middle was caused by bacteria and those knot looking things were mycelium that couldn't grow because of bacteria.  Was I wrong?




thats likely right, but does it really matter? that plate is gross, why do you so desperately want to work with it? if you feel so compelled to try and save it, you could do a hot pour ontop of it. RR has a good explanation of the tek to do it. essentially you pour hot agar on top of it, and watch the plate closely for a couple days, then as soon as you see myc growth on top of the new surface, ideally within a couple hours of the growth you gently scrape it off and put it on a new plate. requires too much babysitting imo tho.... i have better things to do than battle 1 heavily contaminated plate.


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OfflineTira
I'm a teapot


Registered: 11/20/10
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Re: Excess water in no-pour agar plates. [Re: La Flama Blanca]
    #23667610 - 09/22/16 10:22 AM (7 years, 4 months ago)

Quote:

La Flama Blanca said:

thats likely right, but does it really matter? that plate is gross, why do you so desperately want to work with it? if you feel so compelled to try and save it, you could do a hot pour ontop of it. RR has a good explanation of the tek to do it. essentially you pour hot agar on top of it, and watch the plate closely for a couple days, then as soon as you see myc growth on top of the new surface, ideally within a couple hours of the growth you gently scrape it off and put it on a new plate. requires too much babysitting imo tho.... i have better things to do than battle 1 heavily contaminated plate.




like paul stamets' agar sandwich but without a transfer; interesting, thanks for sharing that.

I have other agar plates going on but these are my first plates and I really want to work on agar and get experience.  so why not do transfers from that plate while waiting for growth on others .)


--------------------
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Frank''s Teks          Agar

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Agar for guaranteed spawn,
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Offlinemrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23667656 - 09/22/16 10:48 AM (7 years, 4 months ago)

Its all practice at the end of the day


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OfflineLa Flama Blanca
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Re: Excess water in no-pour agar plates. [Re: Tira]
    #23668111 - 09/22/16 01:28 PM (7 years, 4 months ago)

Quote:

Tira said:
Quote:

La Flama Blanca said:

thats likely right, but does it really matter? that plate is gross, why do you so desperately want to work with it? if you feel so compelled to try and save it, you could do a hot pour ontop of it. RR has a good explanation of the tek to do it. essentially you pour hot agar on top of it, and watch the plate closely for a couple days, then as soon as you see myc growth on top of the new surface, ideally within a couple hours of the growth you gently scrape it off and put it on a new plate. requires too much babysitting imo tho.... i have better things to do than battle 1 heavily contaminated plate.




like paul stamets' agar sandwich but without a transfer; interesting, thanks for sharing that.

I have other agar plates going on but these are my first plates and I really want to work on agar and get experience.  so why not do transfers from that plate while waiting for growth on others .)




up to you man. i think most people, though, would recommend not bothering with advanced techniques like those until you've gotten some solid experience working with agar. building the foundation of the house before adding in the AC and whatnot. but by all means, dive in as deep as you want. :thumbup:


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