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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Excess water in no-pour agar plates.
#23664363 - 09/21/16 08:23 AM (7 years, 4 months ago) |
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Whatever I did, I couldn't get rid of the excess water in my agar plates after PCing.

I even tried putting folded paper towels and wrapping them with foil two times before putting them into PC but didn't do anything for me. Paper towel came out soaking wet and there was water droplets on the lids. And I did raise the temperature of my pressure cooker slowly and left them to cool overnight in the PC.
I know condensation usually is not a problem but these water droplets later drop on the agar and causes the mycelium to drift around on agar even when the plates are carefully handled.
Looks like this:
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BurberrySpores
72 zones



Registered: 08/05/14
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Re: Excess water in no-pour agar plates. [Re: Tira]
#23664525 - 09/21/16 09:49 AM (7 years, 4 months ago) |
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When you take the plates into your SAB, do your sterile procedure, and when you open your lids, just tip the container and dump out the water
I have been doing it this way for a wwwhile. No problems
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mrmazdarx9
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Registered: 05/15/16
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Re: Excess water in no-pour agar plates. [Re: BurberrySpores]
#23664534 - 09/21/16 09:53 AM (7 years, 4 months ago) |
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I don't get what you mean by excess water? All I have is agar on my plates and condensation if I leave them somewhere a little warm, is this just pasty plate related?
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Excess water in no-pour agar plates. [Re: BurberrySpores]
#23664540 - 09/21/16 09:56 AM (7 years, 4 months ago) |
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Quote:
BurberrySpores said: When you take the plates into your SAB, do your sterile procedure, and when you open your lids, just tip the container and dump out the water
I have been doing it this way for a wwwhile. No problems
That may actually work, good idea. But isn't there a way to avoid condensation in the first place? I mean pouring the water out of plates is an extra step and even it sounds safe it does add risk of contamination to the process.
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
#23664545 - 09/21/16 09:58 AM (7 years, 4 months ago) |
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Quote:
mrmazdarx9 said: I don't get what you mean by excess water? All I have is agar on my plates and condensation if I leave them somewhere a little warm, is this just pasty plate related?
Look at the first two pictures in the OP
In the first pic you can see the water droplets on lids and in the second pic you can see condensation on the walls of the plates. That condensation later drips down on agar and then I have water on my agar that drifts spores and mycelium all over.
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BurberrySpores
72 zones



Registered: 08/05/14
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Re: Excess water in no-pour agar plates. [Re: Tira]
#23664559 - 09/21/16 10:04 AM (7 years, 4 months ago) |
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Quote:
Tira said:
Quote:
BurberrySpores said: When you take the plates into your SAB, do your sterile procedure, and when you open your lids, just tip the container and dump out the water
I have been doing it this way for a wwwhile. No problems
That may actually work, good idea. But isn't there a way to avoid condensation in the first place? I mean pouring the water out of plates is an extra step and even it sounds safe it does add risk of contamination to the process.
There's always going to be the excess water, condensation, when doing no pours IME
When you open the lids in the SAB, just tip the container to the side and the water will pour right out.
Its so easy guys...
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mrmazdarx9
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Re: Excess water in no-pour agar plates. [Re: Tira]
#23664563 - 09/21/16 10:05 AM (7 years, 4 months ago) |
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So condensation then, it washes the mycelium off the agar?. How much are you handling the plates for it to drift it all about. I've not had myc move from the agar like you said, is your agar not too runny if the myc is drifting around?
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
#23664567 - 09/21/16 10:07 AM (7 years, 4 months ago) |
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No agar is solid, it's just the water that drifts on it when handled for observation.
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mrmazdarx9
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Registered: 05/15/16
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Re: Excess water in no-pour agar plates. [Re: Tira]
#23664575 - 09/21/16 10:09 AM (7 years, 4 months ago) |
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Tira said:
Quote:
I have water on my agar that drifts spores and mycelium all over.
I'm just going off this
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
#23664583 - 09/21/16 10:12 AM (7 years, 4 months ago) |
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There's no way to prevent condensation in no pour plates, but you can leave them out on the shelf for a while and most of the condensation will dry out/get reabsorbed. If condensation is a problem for you, you might consider pouring petris. It's not as hard or intimidating as it seems, especially if you get the right bottle. Letting your agar cool in the bottle for a while before pouring and then stacking your plates after the pour virtually eliminates condensation on all but the top couple plates.
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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mrmazdarx9
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Re: Excess water in no-pour agar plates. [Re: NumeroEno]
#23664595 - 09/21/16 10:17 AM (7 years, 4 months ago) |
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Quote:
NumeroEno said: There's no way to prevent condensation in no pour plates, but you can leave them out on the shelf for a while and most of the condensation will dry out/get reabsorbed. If condensation is a problem for you, you might consider pouring petris. It's not as hard or intimidating as it seems, especially if you get the right bottle. Letting your agar cool in the bottle for a while before pouring and then stacking your plates after the pour virtually eliminates condensation on all but the top couple plates.
 I bought this bad boy media pouring borosilicate bottle
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
#23664614 - 09/21/16 10:23 AM (7 years, 4 months ago) |
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I was actually thinking about switching to pour agar but was waiting to build a flowhood. I don't know why but I just can't convince myself that I could do that successfully in a SAB.
You make it sound easy, maybe I should give it a try.
(After making a new SAB next week ofc, my current SAB is about 50-60 quarts nowhere near enough space in there. Gonna make a 120 quart one)
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Excess water in no-pour agar plates. [Re: Tira]
#23664626 - 09/21/16 10:30 AM (7 years, 4 months ago) |
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Quote:
Tira said: I was actually thinking about switching to pour agar but was waiting to build a flowhood. I don't know why but I just can't convince myself that I could do that successfully in a SAB.
You make it sound easy, maybe I should give it a try.
(After making a new SAB next week ofc, my current SAB is about 50-60 quarts nowhere near enough space in there. Gonna make a 120 quart one)
I poured plates in my SAB for over a year before I built a flowhood. It's pretty easy honestly. Definitely easier than g2ging into a half gallon jar inside a SAB.
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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mrmazdarx9
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Re: Excess water in no-pour agar plates. [Re: Tira]
#23664628 - 09/21/16 10:31 AM (7 years, 4 months ago) |
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I've had good success with my 80 litre SAB don't know what that is in quarts off hand, but its plenty big enough I've so far only had 4 plates go wrong and im crap lol, two where my very first attempt and I was woefully unequipped.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Excess water in no-pour agar plates. [Re: NumeroEno]
#23664642 - 09/21/16 10:35 AM (7 years, 4 months ago) |
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I've thought about getting a pyrex media bottle for pouring plates, but I still have this thing and it's awesome. I modified the spout a little bit by reaming out the tip to make it bigger, but this thing has kicked ass for almost 2 years now. Picked it up at world market for 10 bucks.
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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spacechildo
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Re: Excess water in no-pour agar plates. [Re: NumeroEno]
#23664668 - 09/21/16 10:48 AM (7 years, 4 months ago) |
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that looks a lot like one of my favorite bongs through the years eno 
lazy mans tek for removing condensation:
1 leave the plates alone for 3 days after PC'ing. 2 nothing, you're done
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Excess water in no-pour agar plates. [Re: mrmazdarx9]
#23664717 - 09/21/16 11:07 AM (7 years, 4 months ago) |
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Quote:
spacechildo said: that looks a lot like one of my favorite bongs through the years eno 
lazy mans tek for removing condensation:
1 leave the plates alone for 3 days after PC'ing. 2 nothing, you're done
my kinda tek .) will definitely try that. thanks a lot.
Quote:
mrmazdarx9 said: I've had good success with my 80 litre SAB don't know what that is in quarts off hand, but its plenty big enough I've so far only had 4 plates go wrong and im crap lol, two where my very first attempt and I was woefully unequipped.
quarts and litre are almost the same as far as I know
thanks a lot guys, you really encouraged me, I think I'm gonna try pour agar soon .)
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mrmazdarx9
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Re: Excess water in no-pour agar plates. [Re: NumeroEno]
#23664733 - 09/21/16 11:17 AM (7 years, 4 months ago) |
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Quote:
NumeroEno said: I've thought about getting a pyrex media bottle for pouring plates, but I still have this thing and it's awesome. I modified the spout a little bit by reaming out the tip to make it bigger, but this thing has kicked ass for almost 2 years now. Picked it up at world market for 10 bucks.

That is cool to be fair
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Excess water in no-pour agar plates. [Re: Tira]
#23664772 - 09/21/16 11:34 AM (7 years, 4 months ago) |
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btw what do you think of these plates, can I do transfers from them? All growth on agar has discoloration beneath them which can be seen clearly from the bottom of the plates. Is that bacteria? (these plates were made from a syringe that had a very high chance of being contaminated I made the syringes two years ago with zero sterile technique

Above is the only proper growth I have on agar that wasn't messed up by water drifting around.

This one is pretty messy but I think there's a clear patch of mycelium at 8 o'clock and maybe at 5 o'clock?

This is the next best plate but it doesn't look very promising.
What do you guys think?
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La Flama Blanca
Stranger


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Re: Excess water in no-pour agar plates. [Re: Tira]
#23664853 - 09/21/16 12:02 PM (7 years, 4 months ago) |
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Quote:
Tira said: btw what do you think of these plates, can I do transfers from them? All growth on agar has discoloration beneath them which can be seen clearly from the bottom of the plates. Is that bacteria? (these plates were made from a syringe that had a very high chance of being contaminated I made the syringes two years ago with zero sterile technique

Above is the only proper growth I have on agar that wasn't messed up by water drifting around.

This one is pretty messy but I think there's a clear patch of mycelium at 8 o'clock and maybe at 5 o'clock?

This is the next best plate but it doesn't look very promising.
What do you guys think?
it helps to know what kind of spores germinated on those plates....not all myc growth is supposed to be white everytime. for example, PS. mexicana
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