Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Bridgetown Botanicals Bridgetown Botanicals   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinejust_curious
Cultivator
Male User Gallery

Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 7 months
Hard to see agar growth
    #23661033 - 09/20/16 09:02 AM (7 years, 5 months ago)

I made a transfer last Sunday from another dish that was made from live tissue. I was attempting to isolate to where there was even growth from the best sectors. The problem is, it has near perfect even growth across the dish, and if some is faster then the rest, I can't see it. BUT, even though the growth is even, there isn't any rhizo at all. The mycelium looks weak. It's just a smooth surface all across the plate as far as I can tell. I almost can't even see the difference between my agar and the mycelium. Is this normal or did I take a bad strain?

I have read a few posts where the mycelium was weak on agar, but really grew out in the grains.

Even if I wanted to take another transfer to another dish, I wouldn't know where to begin because it all looks the exact same, smooth, and no rhizo. Basically a white layer but isn't bacterial.

Is this normal? I wish I could take pics for you guys, but I am using pasty plates and can't get a good photo. I hope I'm explaining it good enough.

Extras: Filter Print Post Top
OfflinePeteyboy
SpaceWalker
Male User Gallery


Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 6 months
Re: Hard to see agar growth [Re: just_curious]
    #23661053 - 09/20/16 09:17 AM (7 years, 5 months ago)

I remember reading somehwere if you made the agar with alot of nutrients it tends to make the mycellium take on a more tomentose form. Weaker nutrients encourage rhizomorphic mycellium.

You could make up a new batch with less potatoe flakes and honey and it might encourage some rhizimorphic development.


--------------------


*TRADE LIST* https://www.shroomery.org/forums/showflat.php/Number/23855008 *TRADE LIST*



Edited by Peteyboy (09/20/16 09:17 AM)

Extras: Filter Print Post Top
Offlinejust_curious
Cultivator
Male User Gallery

Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 7 months
Re: Hard to see agar growth [Re: Peteyboy]
    #23661099 - 09/20/16 09:34 AM (7 years, 5 months ago)

I was thinking that maybe I've been using the same recipe for so long that it was getting used to it. Tomentose was the name I was looking for. I could not remember it for the life of me. If I took an isolated strain from an agar dish made from a clone, and transferred it to a new agar dish, and made a new agar recipe with less nutes, would I even get rhizo growth at that point. Like, you you take pure tomentose mycelium and still pull rhizo froth off of it...especially when you got that growth through isolating?

Extras: Filter Print Post Top
OfflinePeteyboy
SpaceWalker
Male User Gallery


Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 6 months
Re: Hard to see agar growth [Re: just_curious]
    #23661108 - 09/20/16 09:37 AM (7 years, 5 months ago)

I'm no TC by any means brotha, but yes im pretty sure tomentose myc can and will turn to rhizo on agar with less nutes even if from a clone.
  I could be wrong, I'm sure someone much more knowledgeable will chime in shortly!


--------------------


*TRADE LIST* https://www.shroomery.org/forums/showflat.php/Number/23855008 *TRADE LIST*



Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Hard to see agar growth [Re: just_curious]
    #23661142 - 09/20/16 09:52 AM (7 years, 5 months ago)

Hey just_curious, This has been mentioned a bit around the boards lately. It's good practice to rotate agar recipes as well as spawn substrates when working with the same cultures, or in general for that matter.  The variation in nutrients helps keep mycelium strong and healthy. It's also interesting to see how cultures respond to different agar media.  And, as mentioned above, decrease the nutrient content of your agar...I use around 2% and rotate between MEA DFA and oatmeal yeast agar.

Edited by Mycolorado (09/20/16 09:53 AM)

Extras: Filter Print Post Top
Offlinejust_curious
Cultivator
Male User Gallery

Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 7 months
Re: Hard to see agar growth [Re: Mycolorado]
    #23661166 - 09/20/16 09:59 AM (7 years, 5 months ago)

Yeah, I am going to make some DFA. I was just wondering if I should even do another transfer because of how weak the mycelium is. I understood to switch it up, I just didn't realize it would effect it so quickly. The shitty thing is, all my pasty plates are current filled with my standard recipe. I wonder if I could crush up dog food, sprinkle some in each pasty plate I have prepared, and then re-PC so it melts and absorbs into the agar instead of throwing everything out....does anybody know if that would work and be okay?

Edited by just_curious (09/20/16 11:42 AM)

Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Hard to see agar growth [Re: just_curious]
    #23661601 - 09/20/16 12:28 PM (7 years, 5 months ago)

Quote:

just_curious said:
Yeah, I am going to make some DFA. I was just wondering if I should even do another transfer because of how weak the mycelium is. I understood to switch it up, I just didn't realize it would effect it so quickly. The shitty thing is, all my pasty plates are current filled with my standard recipe. I wonder if I could crush up dog food, sprinkle some in each pasty plate I have prepared, and then re-PC so it melts and absorbs into the agar instead of throwing everything out....does anybody know if that would work and be okay?




I'd just wait until you're ready to make new plates.  Adding dog food to your already made plates and re-pc'ing them sounds like a pain in the ass, not to mention you'd really be boosting the nutrient content of your plates which is also appealing to contaminants.


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 4 months
Re: Hard to see agar growth [Re: Mycolorado]
    #23661726 - 09/20/16 01:16 PM (7 years, 5 months ago)

Your clear-looking myc will turn white soon enough. 

Normal.

All of my plates are tomentos but they are still pretty aggressive.  I just pick the thickest/fastest/healthiest looking part.  I'm sure if you look hard enough you will find it.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

Extras: Filter Print Post Top
Offlinejust_curious
Cultivator
Male User Gallery

Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 7 months
Re: Hard to see agar growth [Re: Kenetic]
    #23661834 - 09/20/16 01:49 PM (7 years, 5 months ago)

Well right now I'm wondering if I should do another transfer, or if I should tiger drop to an oats jar and use that to noc up 10 more jars.

Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 4 months
Re: Hard to see agar growth [Re: just_curious]
    #23661852 - 09/20/16 01:56 PM (7 years, 5 months ago)

why not both?


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

Extras: Filter Print Post Top
Offlinejust_curious
Cultivator
Male User Gallery

Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 7 months
Re: Hard to see agar growth [Re: Kenetic]
    #23661885 - 09/20/16 02:06 PM (7 years, 5 months ago)

Because I don't have any other agar prepared and I feel like it's just getting weaker and weaker. If it's already looking this weak, how's it going to be if I transfer more to the same agar recipe. I wonder if it could be as simple as cutting out a quarter of the agar out of the dish, fill the gap with water, PC again so it all melts together, and then let it cool and harden. That way I just diluted the agar to the point where it has less nutes...if that makes sense.

Also, I have a LC oyster syringe and I want to expand it. I could do a SFD and SHIP on a pint jar, make my honey water, PC it, and then inject the jar with the syringe I have already to expand it right?

I have the agar dish in my fridge that I transferred from to make the plate above with the tomentose myc. I want to turn that into LC as well, I'm just trying to figure out the best TEK to do so. Should I just cut out a piece from the dish and add it to a pint with honey water and a SFD & SHIP?

Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 4 months
Re: Hard to see agar growth [Re: just_curious]
    #23661916 - 09/20/16 02:14 PM (7 years, 5 months ago)

If you're gonna go through all that why not just make some new agar? 

I would not recommend spores directly to lc.

As far as how to make your lc, I can't answer that as I don't use lc


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

Extras: Filter Print Post Top
Offlinejust_curious
Cultivator
Male User Gallery

Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 7 months
Re: Hard to see agar growth [Re: Kenetic]
    #23661925 - 09/20/16 02:16 PM (7 years, 5 months ago)

Making a quick LC jar isn't like doing a recipe for agar. But that's not the issue why I can't make agar. My problem is I don't have anymore dishes available and since I have to order my agar online, I really don't want to be wasteful.

Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 4 months
Re: Hard to see agar growth [Re: just_curious]
    #23662011 - 09/20/16 02:42 PM (7 years, 5 months ago)

Well, it seems like your options are limited then.  If your short-term goal is to expand your plate into usable spawn, then do that.  Then you can go from there.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

Extras: Filter Print Post Top
InvisibleJacobStorm
psychedelic cartel
I'm a teapot


Registered: 07/21/15
Posts: 1,499
Re: Hard to see agar growth [Re: Kenetic]
    #23662089 - 09/20/16 03:08 PM (7 years, 5 months ago)

I agree with kenetic. What do you really have too loose? :shrug: if your master jar from that plate doesnt go well couldnt you keep that agar plate in the fridge too try more transfers?


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.

Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: Hard to see agar growth [Re: JacobStorm]
    #23662121 - 09/20/16 03:23 PM (7 years, 5 months ago)

Don't worry about your mycelium, it's not going to shrivel up and die because it's been grown on the same medium a few times...It most likely looks weak as the medium is nutrient-rich.  When mycelium eats nutritious substrates, it moves very slowly as it doesn't have to hurry up and find food.  As mentioned above, just take a transfer and dump the rest on some grain as Kenetic and JS have suggested....sounds like your TC resulted in an isolate from your first post, so you know what you have to look forward to...I guess I'm just confused as to the issue at hand...


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

Edited by Mycolorado (09/20/16 03:43 PM)

Extras: Filter Print Post Top
Offlinejust_curious
Cultivator
Male User Gallery

Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 7 months
Re: Hard to see agar growth [Re: Mycolorado]
    #23662454 - 09/20/16 05:24 PM (7 years, 5 months ago)

Well at first I was just worried why it looked so weak considering I pulled it from a good strain in an attempt to begin isolating. I guess now the issue is wondering if I should continue of transferring because I already made all my pasty plates for this project and they all are the same recipe. I'm going to go ahead and transfer a piece anyways, make LC from a piece, and then tiger drop the rest to some grain. That way I'll have agar continuing (but on plates with lots of nutes) I'll have a master grain, and I'll have a master LC. I just wish I had other glad mini rounds to make a new recipe for the transfers.

Extras: Filter Print Post Top
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Hard to see agar growth [Re: just_curious]
    #23662509 - 09/20/16 05:50 PM (7 years, 5 months ago)

I just learned today that you can possibly solve your problem by removing honey from your agar recipe next time.
Just cooked up 10 new pasty plates today and realized I forgot honey while I was cleaning up so decided to do some research.

I honestly wouldnt worry too much about your mycelium. Ime it will change its look from sub to sub. Mine is usually whispy looking when using pastywhites PDA recipe but once I transfer to grains its a whole different ballgame.

Regarding your LC. I have used honey water with success as well, but word on the streets is that grain soak water or agar mixture sans agar works best.

Good luck man


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself

Extras: Filter Print Post Top
Invisibler.lutece
gave Columbia her wings.
 User Gallery

Registered: 09/06/15
Posts: 745
Loc: ∅
Re: Hard to see agar growth [Re: ComebackKid]
    #23662855 - 09/20/16 07:36 PM (7 years, 5 months ago)

You also said that this was your first transfer from the original tissue, yeah? Even cloned tissue contains several strains. I would try doing another transfer or two until you're 100% sure you have a single-sector isolate before you think about ditching it; it does sometimes take a few isolations for rhizo to show up, even in varied nutrient media.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.

Extras: Filter Print Post Top
Offlinejust_curious
Cultivator
Male User Gallery

Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 7 months
Re: Hard to see agar growth [Re: r.lutece]
    #23662900 - 09/20/16 07:49 PM (7 years, 5 months ago)

Well it was like transfer, transfer, transfer, ect. Grow, LT to agar, transfer 10 more times, another grow, etc. until I would up with this 6 grows later. I started with 1 MS syringe back in March. I'm not sure if at every grow it gets closer, but from what I can tell right now, it's all tomentose and no easily identifiable sectors. It all looks the exact same and grew at the same rate. It is a perfect circle. I will TRY to take a pic for you guys tomorrow.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Bridgetown Botanicals Bridgetown Botanicals   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* how long for agar growth? Anonymous 2,231 2 12/07/02 09:53 AM
by rhizo
* How to make agar 2 agar transfer? esp 6,692 7 01/08/04 11:08 AM
by YidakiMan
* Agar question................ THEBOSS 732 4 02/04/04 09:57 PM
by bluhoney
* transferring agar meatman 2,494 3 10/02/02 01:13 PM
by tripndicular
* agar question muse_sick 710 7 05/17/04 12:22 AM
by ATWAR
* using agar? downforpot 2,217 6 12/02/01 07:00 PM
by Anno
* Agar questions-I may have goofed blahblahblah 3,297 6 01/11/02 02:58 PM
by ar393
* Agar Questions cantara 5,224 6 08/09/01 06:22 AM
by ar393

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
644 topic views. 16 members, 157 guests and 39 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.006 seconds on 12 queries.